SASDAC6: PsrP functional binding region (Functional binding region (187-38...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDAC6

試料

PsrP functional binding region

• Functional binding region (187-385) of the pneumococcal serine-rich repeat protein (protein), PsrP BR(187-385), Streptococcus pneumoniae

機能・相同性

分子機能:

Gram-positive-bacterium-type cell wall / symbiont-mediated perturbation of host defense response / single-species biofilm formation / cell adhesion / cell surface / DNA binding / extracellular region

ドメイン・相同性:

Serine-rich repeat adhesion glycoprotein / : / LPXTG cell wall anchor motif / Gram-positive cocci surface proteins LPxTG motif profile. / LPXTG cell wall anchor domain

構成要素:

Pneumococcal serine-rich repeat protein

• 生物種: Streptococcus pneumoniae (肺炎レンサ球菌)
• 引用: ジャーナル: Sci Rep / 年: 2016; タイトル: The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.; 著者: Tim Schulte / Cecilia Mikaelsson / Audrey Beaussart / Alexey Kikhney / Maya Deshmukh / Sebastian Wolniak / Anuj Pathak / Christine Ebel / Jonas Löfling / Federico Fogolari / Birgitta Henriques-Normark / Yves F Dufrêne / Dmitri Svergun / Per-Åke Nygren / Adnane Achour / ; 要旨: The major human pathogen Streptococcus pneumoniae is a leading cause of disease and death worldwide. Pneumococcal biofilm formation within the nasopharynx leads to long-term colonization and persistence within the host. We have previously demonstrated that the capsular surface-associated pneumococcal serine rich repeat protein (PsrP), key factor for biofilm formation, binds to keratin-10 (KRT10) through its microbial surface component recognizing adhesive matrix molecule (MSCRAMM)-related globular binding region domain (BR187-385). Here, we show that BR187-385 also binds to DNA, as demonstrated by electrophoretic mobility shift assays and size exclusion chromatography. Further, heterologous expression of BR187-378 or the longer BR120-378 construct on the surface of a Gram-positive model host bacterium resulted in the formation of cellular aggregates that was significantly enhanced in the presence of DNA. Crystal structure analyses revealed the formation of BR187-385 homo-dimers via an intermolecular β-sheet, resulting in a positively charged concave surface, shaped to accommodate the acidic helical DNA structure. Furthermore, small angle X-ray scattering and circular dichroism studies indicate that the aggregate-enhancing N-terminal region of BR120-166 adopts an extended, non-globular structure. Altogether, our results suggest that PsrP adheres to extracellular DNA in the biofilm matrix and thus promotes pneumococcal biofilm formation.

登録者

• Al Kikhney (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #565: ; タイプ: mix / ソフトウェア: EOM (1.3) / ダミー原子の半径: 1.90 A / カイ2乗値: 0.974169; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #566: ; タイプ: mix / ソフトウェア: EOM (1.4) / ダミー原子の半径: 1.90 A / カイ2乗値: 0.974169; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: PsrP functional binding region / 試料濃度: 2.9 mg/ml
• バッファ: 名称: PBS / pH: 7.4 / コメント: Phosphate buffered saline / 組成: Glycerol 5.000 %

要素 #37

名称: PsrP BR(187-385) / タイプ: protein
記述: Functional binding region (187-385) of the pneumococcal serine-rich repeat protein
分子量: 22.12 / 分子数: 1 / 由来: Streptococcus pneumoniae / 参照: UniProt: A0A0H2URK1
配列:

HHHHHHSGNT IVNGAPAINA SLNIAKSETK VYTGEGVDSV YRVPIYYKLK VTNDGSKLTF TYTVTYVNPK TNDLGNISSM RPGYSIYNSG TSTQTMLTLG SDLGKPSGVK NYITDKNGRQ VLSYNTSTMT TQGSGYTWGN GAQMNGFFAK KGYGLTSSWT VPITGTDTSF TFTPYAARTD RIGINYFNGG GKVVESSTTS QSLSQ

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.09919 Å / スペクトロメータ・検出器間距離: 2.43 mm
• 検出器: 名称: Pilatus 1M

スキャン

タイトル: PsrP functional binding region / 測定日: 2013年2月27日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /

	Min	Max
Q	0.0716	4.4209

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 429 /

	Min	Max
Q	0.2387	3.952
P(R) point	49	477
R	0	7.8

結果

カーブのタイプ: single_conc / Standard: BSA

	実験値	Standard	Porod
分子量	18 kDa	17 kDa	23.6 kDa
体積	-	-	37.7 nm3

	P(R)	Guinier
前方散乱 I0	18.64	18.511
慣性半径, Rg	2.11 nm	2.039 nm

	Min	Max
D	-	7.8
Guinier point	40	108