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- SASDAC6: PsrP functional binding region (Functional binding region (187-38... -

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Basic information

Entry
Database: SASBDB / ID: SASDAC6
SamplePsrP functional binding region
  • Functional binding region (187-385) of the pneumococcal serine-rich repeat protein (protein), PsrP BR(187-385), Streptococcus pneumoniae
Function / homology
Function and homology information


Gram-positive-bacterium-type cell wall / symbiont-mediated perturbation of host defense response / single-species biofilm formation / cell adhesion / cell surface / DNA binding / extracellular region
Similarity search - Function
Serine-rich repeat adhesion glycoprotein / LPXTG cell wall anchor motif / Gram-positive cocci surface proteins LPxTG motif profile. / LPXTG cell wall anchor domain
Similarity search - Domain/homology
Pneumococcal serine-rich repeat protein
Similarity search - Component
Biological speciesStreptococcus pneumoniae (bacteria)
CitationJournal: Sci Rep / Year: 2016
Title: The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.
Authors: Tim Schulte / Cecilia Mikaelsson / Audrey Beaussart / Alexey Kikhney / Maya Deshmukh / Sebastian Wolniak / Anuj Pathak / Christine Ebel / Jonas Löfling / Federico Fogolari / Birgitta ...Authors: Tim Schulte / Cecilia Mikaelsson / Audrey Beaussart / Alexey Kikhney / Maya Deshmukh / Sebastian Wolniak / Anuj Pathak / Christine Ebel / Jonas Löfling / Federico Fogolari / Birgitta Henriques-Normark / Yves F Dufrêne / Dmitri Svergun / Per-Åke Nygren / Adnane Achour /
Abstract: The major human pathogen Streptococcus pneumoniae is a leading cause of disease and death worldwide. Pneumococcal biofilm formation within the nasopharynx leads to long-term colonization and ...The major human pathogen Streptococcus pneumoniae is a leading cause of disease and death worldwide. Pneumococcal biofilm formation within the nasopharynx leads to long-term colonization and persistence within the host. We have previously demonstrated that the capsular surface-associated pneumococcal serine rich repeat protein (PsrP), key factor for biofilm formation, binds to keratin-10 (KRT10) through its microbial surface component recognizing adhesive matrix molecule (MSCRAMM)-related globular binding region domain (BR187-385). Here, we show that BR187-385 also binds to DNA, as demonstrated by electrophoretic mobility shift assays and size exclusion chromatography. Further, heterologous expression of BR187-378 or the longer BR120-378 construct on the surface of a Gram-positive model host bacterium resulted in the formation of cellular aggregates that was significantly enhanced in the presence of DNA. Crystal structure analyses revealed the formation of BR187-385 homo-dimers via an intermolecular β-sheet, resulting in a positively charged concave surface, shaped to accommodate the acidic helical DNA structure. Furthermore, small angle X-ray scattering and circular dichroism studies indicate that the aggregate-enhancing N-terminal region of BR120-166 adopts an extended, non-globular structure. Altogether, our results suggest that PsrP adheres to extracellular DNA in the biofilm matrix and thus promotes pneumococcal biofilm formation.
Contact author
  • Al Kikhney (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #565
Type: mix / Software: EOM (1.3) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.974169
Search similar-shape structures of this assembly by Omokage search (details)
Model #566
Type: mix / Software: EOM (1.4) / Radius of dummy atoms: 1.90 A / Chi-square value: 0.974169
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: PsrP functional binding region / Specimen concentration: 2.9 mg/ml
BufferName: PBS / pH: 7.4 / Comment: Phosphate buffered saline / Composition: Glycerol 5.000 %
Entity #37Name: PsrP BR(187-385) / Type: protein
Description: Functional binding region (187-385) of the pneumococcal serine-rich repeat protein
Formula weight: 22.12 / Num. of mol.: 1 / Source: Streptococcus pneumoniae / References: UniProt: A0A0H2URK1
Sequence: HHHHHHSGNT IVNGAPAINA SLNIAKSETK VYTGEGVDSV YRVPIYYKLK VTNDGSKLTF TYTVTYVNPK TNDLGNISSM RPGYSIYNSG TSTQTMLTLG SDLGKPSGVK NYITDKNGRQ VLSYNTSTMT TQGSGYTWGN GAQMNGFFAK KGYGLTSSWT VPITGTDTSF ...Sequence:
HHHHHHSGNT IVNGAPAINA SLNIAKSETK VYTGEGVDSV YRVPIYYKLK VTNDGSKLTF TYTVTYVNPK TNDLGNISSM RPGYSIYNSG TSTQTMLTLG SDLGKPSGVK NYITDKNGRQ VLSYNTSTMT TQGSGYTWGN GAQMNGFFAK KGYGLTSSWT VPITGTDTSF TFTPYAARTD RIGINYFNGG GKVVESSTTS QSLSQ

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / : France / Type of source: X-ray synchrotron / Wavelength: 0.09919 Å / Dist. spec. to detc.: 2.43 mm
DetectorName: Pilatus 1M
Scan
Title: PsrP functional binding region / Measurement date: Feb 27, 2013 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.0716 4.4209
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 429 /
MinMax
Q0.2387 3.952
P(R) point49 477
R0 7.8
Result
Type of curve: single_conc / Standard: BSA
ExperimentalStandardPorod
MW18 kDa17 kDa23.6 kDa
Volume--37.7 nm3

P(R)Guinier
Forward scattering, I018.64 18.511
Radius of gyration, Rg2.11 nm2.039 nm

MinMax
D-7.8
Guinier point40 108

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