+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDA89 |
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Sample | Complex between ovine GM-CSF and GM-CSF/IL-2 inhibition factor
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Function / homology | Function and homology information granulocyte macrophage colony-stimulating factor receptor binding / neutrophil differentiation / positive regulation of interleukin-23 production / positive regulation of macrophage derived foam cell differentiation / myeloid dendritic cell differentiation / granulocyte macrophage colony-stimulating factor receptor complex / granulocyte-macrophage colony-stimulating factor signaling pathway / positive regulation of leukocyte proliferation / cellular response to granulocyte macrophage colony-stimulating factor stimulus / positive regulation of podosome assembly ...granulocyte macrophage colony-stimulating factor receptor binding / neutrophil differentiation / positive regulation of interleukin-23 production / positive regulation of macrophage derived foam cell differentiation / myeloid dendritic cell differentiation / granulocyte macrophage colony-stimulating factor receptor complex / granulocyte-macrophage colony-stimulating factor signaling pathway / positive regulation of leukocyte proliferation / cellular response to granulocyte macrophage colony-stimulating factor stimulus / positive regulation of podosome assembly / monocyte differentiation / cell surface receptor signaling pathway via JAK-STAT / macrophage differentiation / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / embryonic placenta development / positive regulation of tyrosine phosphorylation of STAT protein / cytokine activity / growth factor activity / cell population proliferation / immune response / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / extracellular space Similarity search - Function |
Biological species | Orf virus Ovis aries (sheep) |
Citation | Date: 2016 Nov 7 Title: Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF Authors: Felix J / Kandiah E / De Munck S / Bloch Y / van Zundert G / Pauwels K / Dansercoer A / Novanska K / Read R / Bonvin A / Vergauwen B / Verstraete K / Gutsche I |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Data source
SASBDB page | SASDA89 |
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-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data |
-External links
Related items in Molecule of the Month |
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-Models
Model #353 | Type: mix / Software: FoXS / Radius of dummy atoms: 1.90 A / Chi-square value: 183.782981843 Search similar-shape structures of this assembly by Omokage search (details) |
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-Sample
Sample | Name: Complex between ovine GM-CSF and GM-CSF/IL-2 inhibition factor Purity method: SEC-SAXS / Specimen concentration: 9.7 mg/ml / Entity id: 209 / 210 |
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Buffer | Name: HEPES / Concentration: 20.00 mM / pH: 7.4 / Composition: 150 mM NaCl |
Entity #209 | Type: protein / Description: GM-CSF/IL-2 inhibition factor / Formula weight: 29.924 / Num. of mol.: 4 / Source: Orf virus / References: UniProt: Q9J5U5 Sequence: MACLRVFLAV LALCGSVHSA QWIGERDFCT AHAQDVFARL QVWMRIDRNV TAADNSSACA LAIETPPSNF DADVYVAAAG INVSVSAINC GFFNMRQVET TYNTARRQMY VYMDSWDPWV IDDPQPLFSQ EYENETLPYL LEVLELARLY IRVGCTVPGE QPFEVIPGID ...Sequence: MACLRVFLAV LALCGSVHSA QWIGERDFCT AHAQDVFARL QVWMRIDRNV TAADNSSACA LAIETPPSNF DADVYVAAAG INVSVSAINC GFFNMRQVET TYNTARRQMY VYMDSWDPWV IDDPQPLFSQ EYENETLPYL LEVLELARLY IRVGCTVPGE QPFEVIPGID YPHTGMEFLQ HVLRPNRRFA PAKLHMDLEV DHRCVSAVHV KAFLQDACSA RKARTPLYFA GHGCNHPDRR PKNPVPRPQH VSSPISRKCS MQTAR |
Entity #210 | Type: protein Description: Granulocyte-macrophage colony-stimulating factor Formula weight: 14.411 / Num. of mol.: 2 / Source: Ovis aries / References: UniProt: P28773 Sequence: APTRQPSPVT RPWQHVDAIK EALSLLNDST DTAAVMDETV EVVSEMFDSQ EPTCLQTRLE LYKQGLRGSL TSLTGSLTMM ASHYKKHCPP TQETSCETQI ITFKSFKENL KDFLFIIPFD CWEPVQK |
-Experimental information
Beam | Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.82 mm | |||||||||||||||||||||||||||||||||
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Detector | Name: AVIEX / Type: CCD | |||||||||||||||||||||||||||||||||
Scan | Title: Complex between ovine GM-CSF and GIF / Measurement date: Sep 10, 2014 / Cell temperature: 20 °C / Number of frames: 16 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 464 /
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Result | Type of curve: other Comments: Modeling of SAXS data: The crystal structure of the GIF:oGM-CSF complex was used as an input for the Allosmod-FoXS web server to model missing loops, N- and C-termini and N-linked ...Comments: Modeling of SAXS data: The crystal structure of the GIF:oGM-CSF complex was used as an input for the Allosmod-FoXS web server to model missing loops, N- and C-termini and N-linked glycosylation. During each Allosmod-FoXS run, data up to a scattering angle of 0.5 Å-1 was used. Fits to the experimental SAXS data were calculated using the FoXS software.
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