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- PDB-4js5: Crystal structure of E. coli Exonuclease I in complex with a dT13... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4js5 | ||||||
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Title | Crystal structure of E. coli Exonuclease I in complex with a dT13 oligonucleotide | ||||||
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![]() | HYDROLASE/DNA / DNA repair / exonuclease / processive / DnaQ superfamily / 3'-5' ssDNA exonuclease / HYDROLASE / HYDROLASE-DNA complex | ||||||
Function / homology | ![]() exodeoxyribonuclease I / single-stranded DNA 3'-5' DNA exonuclease activity / DNA catabolic process / 5'-deoxyribose-5-phosphate lyase activity / single-stranded DNA binding / 3'-5'-RNA exonuclease activity / DNA repair / magnesium ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bell, C.E. | ||||||
![]() | ![]() Title: Crystal structures of Escherichia coli exonuclease I in complex with single-stranded DNA provide insights into the mechanism of processive digestion. Authors: Korada, S.K. / Johns, T.D. / Smith, C.E. / Jones, N.D. / McCabe, K.A. / Bell, C.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 205.4 KB | Display | ![]() |
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PDB format | ![]() | 164.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 466.9 KB | Display | ![]() |
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Full document | ![]() | 474.3 KB | Display | |
Data in XML | ![]() | 32.8 KB | Display | |
Data in CIF | ![]() | 44.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4jrpC ![]() 4jrqC ![]() 4js4C ![]() 1fxxS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: DNA chain | Mass: 3909.549 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: chemical synthesis #2: Protein | Mass: 54848.891 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: N-terminal 6His-thrombin tab / Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.65 Å3/Da / Density % sol: 73.55 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 5% 2-propanol, 25 % glycerol, 1.2 M ammonium sulfate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX225HE / Detector: CCD / Date: Nov 16, 2012 |
Radiation | Monochromator: Kohzu HLD-4 double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97931 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→49 Å / Num. all: 26653 / Num. obs: 26653 / % possible obs: 99.9 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 11 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 9.6 |
Reflection shell | Resolution: 3.5→3.69 Å / Redundancy: 10.1 % / Rmerge(I) obs: 0.786 / Mean I/σ(I) obs: 2 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1FXX Resolution: 3.5→49 Å / Cor.coef. Fo:Fc: 0.93 / Cor.coef. Fo:Fc free: 0.909 / SU B: 61.309 / SU ML: 0.789 / Cross valid method: THROUGHOUT / σ(F): 1 / σ(I): 1 / ESU R Free: 0.67 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 196.357 Å2
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Refinement step | Cycle: LAST / Resolution: 3.5→49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.5→3.591 Å / Total num. of bins used: 20
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