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- SASDA89: Complex between ovine GM-CSF and GM-CSF/IL-2 inhibition factor -

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Basic information

Entry
Database: SASBDB / ID: SASDA89
SampleComplex between ovine GM-CSF and GM-CSF/IL-2 inhibition factor
  • GM-CSF/IL-2 inhibition factor (protein), Orf virus
  • Granulocyte-macrophage colony-stimulating factor (protein), Ovis aries
Function / homology
Function and homology information


granulocyte macrophage colony-stimulating factor receptor binding / neutrophil differentiation / positive regulation of interleukin-23 production / positive regulation of macrophage derived foam cell differentiation / myeloid dendritic cell differentiation / granulocyte macrophage colony-stimulating factor receptor complex / granulocyte-macrophage colony-stimulating factor signaling pathway / positive regulation of leukocyte proliferation / cellular response to granulocyte macrophage colony-stimulating factor stimulus / positive regulation of podosome assembly ...granulocyte macrophage colony-stimulating factor receptor binding / neutrophil differentiation / positive regulation of interleukin-23 production / positive regulation of macrophage derived foam cell differentiation / myeloid dendritic cell differentiation / granulocyte macrophage colony-stimulating factor receptor complex / granulocyte-macrophage colony-stimulating factor signaling pathway / positive regulation of leukocyte proliferation / cellular response to granulocyte macrophage colony-stimulating factor stimulus / positive regulation of podosome assembly / monocyte differentiation / cell surface receptor signaling pathway via JAK-STAT / macrophage differentiation / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / embryonic placenta development / positive regulation of tyrosine phosphorylation of STAT protein / cytokine activity / growth factor activity / cell population proliferation / immune response / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / extracellular space
Similarity search - Function
Poxvirus chemokine inhibitor superfamily / Granulocyte-macrophage colony-stimulating factor / Granulocyte-macrophage colony-stimulating factor / Granulocyte-macrophage colony-stimulating factor signature. / Granulocyte-macrophage colony-simulating factor (GM-CSF) / Four-helical cytokine-like, core
Similarity search - Domain/homology
Granulocyte-macrophage colony-stimulating factor / GM-CSF/IL-2 inhibition factor
Similarity search - Component
Biological speciesOrf virus
Ovis aries (sheep)
CitationDate: 2016 Nov 7
Title: Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF
Authors: Felix J / Kandiah E / De Munck S / Bloch Y / van Zundert G / Pauwels K / Dansercoer A / Novanska K / Read R / Bonvin A / Vergauwen B / Verstraete K / Gutsche I
Contact author
  • Jan Felix

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #353
Type: mix / Software: FoXS / Radius of dummy atoms: 1.90 A / Chi-square value: 183.782981843
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Complex between ovine GM-CSF and GM-CSF/IL-2 inhibition factor
Purity method: SEC-SAXS / Specimen concentration: 9.7 mg/ml / Entity id: 209 / 210
BufferName: HEPES / Concentration: 20.00 mM / pH: 7.4 / Composition: 150 mM NaCl
Entity #209Type: protein / Description: GM-CSF/IL-2 inhibition factor / Formula weight: 29.924 / Num. of mol.: 4 / Source: Orf virus / References: UniProt: Q9J5U5
Sequence: MACLRVFLAV LALCGSVHSA QWIGERDFCT AHAQDVFARL QVWMRIDRNV TAADNSSACA LAIETPPSNF DADVYVAAAG INVSVSAINC GFFNMRQVET TYNTARRQMY VYMDSWDPWV IDDPQPLFSQ EYENETLPYL LEVLELARLY IRVGCTVPGE QPFEVIPGID ...Sequence:
MACLRVFLAV LALCGSVHSA QWIGERDFCT AHAQDVFARL QVWMRIDRNV TAADNSSACA LAIETPPSNF DADVYVAAAG INVSVSAINC GFFNMRQVET TYNTARRQMY VYMDSWDPWV IDDPQPLFSQ EYENETLPYL LEVLELARLY IRVGCTVPGE QPFEVIPGID YPHTGMEFLQ HVLRPNRRFA PAKLHMDLEV DHRCVSAVHV KAFLQDACSA RKARTPLYFA GHGCNHPDRR PKNPVPRPQH VSSPISRKCS MQTAR
Entity #210Type: protein
Description: Granulocyte-macrophage colony-stimulating factor
Formula weight: 14.411 / Num. of mol.: 2 / Source: Ovis aries / References: UniProt: P28773
Sequence:
APTRQPSPVT RPWQHVDAIK EALSLLNDST DTAAVMDETV EVVSEMFDSQ EPTCLQTRLE LYKQGLRGSL TSLTGSLTMM ASHYKKHCPP TQETSCETQI ITFKSFKENL KDFLFIIPFD CWEPVQK

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Experimental information

BeamInstrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.82 mm
DetectorName: AVIEX / Type: CCD
Scan
Title: Complex between ovine GM-CSF and GIF / Measurement date: Sep 10, 2014 / Cell temperature: 20 °C / Number of frames: 16 / Unit: 1/nm /
MinMax
Q0.0761 5.4962
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 464 /
MinMax
Q0.0157228 0.250391
P(R) point1 464
R0 124
Result
Type of curve: other
Comments: Modeling of SAXS data: The crystal structure of the GIF:oGM-CSF complex was used as an input for the Allosmod-FoXS web server to model missing loops, N- and C-termini and N-linked ...Comments: Modeling of SAXS data: The crystal structure of the GIF:oGM-CSF complex was used as an input for the Allosmod-FoXS web server to model missing loops, N- and C-termini and N-linked glycosylation. During each Allosmod-FoXS run, data up to a scattering angle of 0.5 Å-1 was used. Fits to the experimental SAXS data were calculated using the FoXS software.
ExperimentalStandardPorod
MW153 kDa153 kDa144 kDa
Volume--231 nm3

P(R)GuinierGuinier error
Forward scattering, I00.1494 0.151381 0.0001
Radius of gyration, Rg3.77 nm3.79 nm0.13

MinMax
D-12.4
Guinier point17 47

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