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- PDB-9mog: Structure of the distal part of the bacteriophage T4 tail -

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Basic information

Entry
Database: PDB / ID: 9mog
TitleStructure of the distal part of the bacteriophage T4 tail
Components
  • (unassigned peptide ...) x 5
  • gp10, baseplate wedge protein
  • gp11, baseplate wedge protein
  • gp12, short tail fiber protein
  • gp18, tail sheath protein
  • gp19, tail tube protein
  • gp25, baseplate wedge protein
  • gp27, baseplate hub protein
  • gp29, tape measure protein
  • gp48, baseplate protein
  • gp5, baseplate central spike protein
  • gp5.4, tip of the central spike
  • gp53, baseplate wedge protein
  • gp54, baseplate protein
  • gp6, baseplate wedge protein
  • gp7, baseplate wedge protein
  • gp8, baseplate wedge protein
  • gp9, baseplate wedge tail fiber connector
KeywordsVIRAL PROTEIN / bacteriophage T4 / contractile tail / baseplate
Function / homology
Function and homology information


virus tail, sheath / symbiont genome ejection through host cell envelope, contractile tail mechanism / symbiont entry into host cell via disruption of host cell wall peptidoglycan / virus tail, baseplate / viral tail assembly / virus tail, fiber / symbiont entry into host cell via disruption of host cell envelope / viral release from host cell / peptidoglycan catabolic process / virion component ...virus tail, sheath / symbiont genome ejection through host cell envelope, contractile tail mechanism / symbiont entry into host cell via disruption of host cell wall peptidoglycan / virus tail, baseplate / viral tail assembly / virus tail, fiber / symbiont entry into host cell via disruption of host cell envelope / viral release from host cell / peptidoglycan catabolic process / virion component / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / entry receptor-mediated virion attachment to host cell / defense response to bacterium / symbiont entry into host cell / structural molecule activity / metal ion binding / identical protein binding
Similarity search - Function
Bacteriophage T4, Gp12 / Baseplate tail-tube protein gp48, T4-like virus / Short tail fibre protein, C-terminal / Short tail fibre protein, C-terminal superfamily / Phage short tail fibre protein gp12, middle domain / Tail-tube assembly protein / Short tail fibre protein receptor-binding domain / Baseplate wedge protein gp53, bacteriophage T4 / Baseplate wedge protein gp7 / : ...Bacteriophage T4, Gp12 / Baseplate tail-tube protein gp48, T4-like virus / Short tail fibre protein, C-terminal / Short tail fibre protein, C-terminal superfamily / Phage short tail fibre protein gp12, middle domain / Tail-tube assembly protein / Short tail fibre protein receptor-binding domain / Baseplate wedge protein gp53, bacteriophage T4 / Baseplate wedge protein gp7 / : / : / : / Base plate wedge protein 53 / Baseplate wedge protein gp7, domain V / Baseplate wedge protein gp7, helical domain / Baseplate wedge protein gp7, domain VI / Bacteriophage T4, Gp27, baseplate hub, C-terminal / Bacteriophage T4, Gp27, baseplate hub, N-terminal / Gp27, baseplate hub, domain 3 / Gp27, baseplate hub, domain 4 / Gp27, baseplate hub, domain 2 / Baseplate structural protein, domain 2 / Baseplate structural protein, domain 1 / Baseplate structural protein Gp11 / Bacteriophage T4, Gp11, C-terminal finger domain / Baseplate structural protein Gp11, N-terminal domain superfamily / Baseplate structural protein Gp11 superfamily / Baseplate structural protein Gp11, C-terminal domain / GP11 baseplate wedge protein / Baseplate wedge protein gp10 / Baseplate wedge protein gp6 / Phage tail collar domain superfamily / : / : / : / : / : / : / Baseplate wedge protein gp6-like, helical domain / Baseplate structural protein gp6, C-terminal domain I / Baseplate structural protein gp6, C-terminal domain II / Baseplate wedge protein gp6, domain II / Baseplate structural protein gp6, C-terminal domain III / Baseplate wedge protein gp10 domain 3 / : / Tail sheath protein Gp18-like domain I / Phage tail collar domain / Baseplate structural protein Gp9 C-terminal domain superfamily / Phage Tail Collar Domain / Bacteriophage T4, Gp8 / Bacteriophage T4, Gp8 superfamily / Bacteriophage T4, Gp8 / Gp5, C-terminal / Gp5 C-terminal repeat (3 copies) / : / Baseplate structural protein Gp10, C-terminal domain / Baseplate structural protein Gp9/Gp10 / Baseplate structural protein Gp9/Gp10 middle domain superfamily / Gp9-like superfamily / : / Bacteriophage T4 gp9/10-like protein, N-terminal / Bacteriophage T4 gp9/10-like protein, C-terminal / Protein Gp5, N-terminal OB-fold domain / Pre-baseplate central spike protein Gp5 / Gp5 N-terminal OB domain / : / Tail sheath protein Gp18 domain III N-terminal region / IraD/Gp25-like / PAAR motif / Baseplate wedge protein gp25 / PAAR motif / Phage tail sheath protein, beta-sandwich domain / Phage tail sheath protein beta-sandwich domain / Bacteriophage T4, Gp19, tail tube / T4-like virus tail tube protein gp19 / : / Tail sheath protein, subtilisin-like domain / Phage tail sheath protein subtilisin-like domain / Tail sheath protein, C-terminal domain / Phage tail sheath C-terminal domain / T4-type lysozyme / : / Glycoside hydrolase, family 24 / Phage lysozyme / Lysozyme domain superfamily / Fibronectin type III / Fibronectin type III superfamily / Lysozyme-like domain superfamily / Immunoglobulin-like fold / Peptidase S1, PA clan, chymotrypsin-like fold
Similarity search - Domain/homology
Pre-baseplate central spike protein Gp5 / Tail tube protein / Phospholipase / Baseplate tail tube cap / Tail sheath protein / Baseplate wedge subunit / Baseplate wedge subunit and tail pin / Baseplate wedge subunit / Baseplate wedge tail fiber connector / Baseplate hub subunit ...Pre-baseplate central spike protein Gp5 / Tail tube protein / Phospholipase / Baseplate tail tube cap / Tail sheath protein / Baseplate wedge subunit / Baseplate wedge subunit and tail pin / Baseplate wedge subunit / Baseplate wedge tail fiber connector / Baseplate hub subunit / Baseplate wedge subunit / Baseplate wedge protein gp10 / Short tail fiber protein gp12 / Tape measure protein / Baseplate tail-tube junction protein gp54 / Baseplate wedge protein gp6 / Baseplate wedge protein gp7
Similarity search - Component
Biological speciesEscherichia phage T4 (virus)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsFokine, A. / Zhu, J. / Klose, T. / Vago, F. / Arnaud, C. / Wang, Z. / Khare, B. / Rossmann, M.G. / Chen, Z. / Sun, L. ...Fokine, A. / Zhu, J. / Klose, T. / Vago, F. / Arnaud, C. / Wang, Z. / Khare, B. / Rossmann, M.G. / Chen, Z. / Sun, L. / Fang, Q. / Kuhn, R. / Rao, V.B.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI081726 United States
CitationJournal: To Be Published
Title: In situ Atomic Structure of Phage T4 Head-Tail Connector Complex Shows Molecular Gates Open for Genome Ejection and DNA Pushed into the Tail Tube
Authors: Fokine, A. / Zhu, J. / Klose, T. / Vago, F. / Arnaud, C. / Wang, Z. / Khare, B. / Rossmann, M.G. / Chen, Z. / Sun, L. / Fang, Q. / Kuhn, R. / Rao, V.B.
History
DepositionDec 26, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 28, 2026Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: Mask / Part number: 1 / Data content type: Mask / Provider: repository / Type: Initial release
Revision 1.0Jan 28, 2026Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

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Assembly

Deposited unit
S0: gp12, short tail fiber protein
S1: gp12, short tail fiber protein
S2: gp12, short tail fiber protein
S3: gp12, short tail fiber protein
S4: gp12, short tail fiber protein
S5: gp12, short tail fiber protein
S6: gp12, short tail fiber protein
S7: gp12, short tail fiber protein
S8: gp12, short tail fiber protein
SA: gp12, short tail fiber protein
SO: gp12, short tail fiber protein
SP: gp12, short tail fiber protein
SQ: gp12, short tail fiber protein
Sv: gp12, short tail fiber protein
Sw: gp12, short tail fiber protein
Sx: gp12, short tail fiber protein
Sy: gp12, short tail fiber protein
Sz: gp12, short tail fiber protein
T1: gp9, baseplate wedge tail fiber connector
T2: gp9, baseplate wedge tail fiber connector
T3: gp9, baseplate wedge tail fiber connector
T4: gp9, baseplate wedge tail fiber connector
T5: gp9, baseplate wedge tail fiber connector
T6: gp9, baseplate wedge tail fiber connector
T7: gp9, baseplate wedge tail fiber connector
T8: gp9, baseplate wedge tail fiber connector
T9: gp9, baseplate wedge tail fiber connector
TF: gp9, baseplate wedge tail fiber connector
TG: gp9, baseplate wedge tail fiber connector
TH: gp9, baseplate wedge tail fiber connector
Td: gp9, baseplate wedge tail fiber connector
Te: gp9, baseplate wedge tail fiber connector
Tf: gp9, baseplate wedge tail fiber connector
Tg: gp9, baseplate wedge tail fiber connector
Th: gp9, baseplate wedge tail fiber connector
Ti: gp9, baseplate wedge tail fiber connector
UL: gp11, baseplate wedge protein
UM: gp11, baseplate wedge protein
UN: gp11, baseplate wedge protein
Up: gp11, baseplate wedge protein
Uq: gp11, baseplate wedge protein
Ur: gp11, baseplate wedge protein
Us: gp11, baseplate wedge protein
Ut: gp11, baseplate wedge protein
Uu: gp11, baseplate wedge protein
VL: gp11, baseplate wedge protein
VM: gp11, baseplate wedge protein
VN: gp11, baseplate wedge protein
Vp: gp11, baseplate wedge protein
Vq: gp11, baseplate wedge protein
Vr: gp11, baseplate wedge protein
Vs: gp11, baseplate wedge protein
Vt: gp11, baseplate wedge protein
Vu: gp11, baseplate wedge protein
WI: gp10, baseplate wedge protein
WJ: gp10, baseplate wedge protein
WK: gp10, baseplate wedge protein
Wj: gp10, baseplate wedge protein
Wk: gp10, baseplate wedge protein
Wl: gp10, baseplate wedge protein
Wm: gp10, baseplate wedge protein
Wn: gp10, baseplate wedge protein
Wo: gp10, baseplate wedge protein
XI: gp10, baseplate wedge protein
XJ: gp10, baseplate wedge protein
XK: gp10, baseplate wedge protein
Xj: gp10, baseplate wedge protein
Xk: gp10, baseplate wedge protein
Xl: gp10, baseplate wedge protein
Xm: gp10, baseplate wedge protein
Xn: gp10, baseplate wedge protein
Xo: gp10, baseplate wedge protein
YC: gp7, baseplate wedge protein
YX: gp7, baseplate wedge protein
YY: gp7, baseplate wedge protein
Yc: gp7, baseplate wedge protein
Yx: gp7, baseplate wedge protein
Yy: gp7, baseplate wedge protein
ZD: gp8, baseplate wedge protein
ZE: gp8, baseplate wedge protein
ZZ: gp8, baseplate wedge protein
Za: gp8, baseplate wedge protein
Zb: gp8, baseplate wedge protein
Zc: gp8, baseplate wedge protein
aD: gp8, baseplate wedge protein
aE: gp8, baseplate wedge protein
aZ: gp8, baseplate wedge protein
aa: gp8, baseplate wedge protein
ab: gp8, baseplate wedge protein
ac: gp8, baseplate wedge protein
b0: gp18, tail sheath protein
b3: gp18, tail sheath protein
b4: gp18, tail sheath protein
b5: gp18, tail sheath protein
b6: gp18, tail sheath protein
b7: gp18, tail sheath protein
b8: gp18, tail sheath protein
b9: gp18, tail sheath protein
bN: gp18, tail sheath protein
bO: gp18, tail sheath protein
bP: gp18, tail sheath protein
bQ: gp18, tail sheath protein
bR: gp18, tail sheath protein
bY: gp18, tail sheath protein
cA: gp18, tail sheath protein
cB: gp18, tail sheath protein
cC: gp18, tail sheath protein
cD: gp18, tail sheath protein
cE: gp18, tail sheath protein
cF: gp18, tail sheath protein
cG: gp18, tail sheath protein
cH: gp18, tail sheath protein
cI: gp18, tail sheath protein
cJ: gp18, tail sheath protein
cK: gp18, tail sheath protein
cL: gp18, tail sheath protein
cM: gp18, tail sheath protein
cN: gp18, tail sheath protein
cO: gp18, tail sheath protein
cP: gp18, tail sheath protein
dS: gp18, tail sheath protein
dT: gp18, tail sheath protein
dU: gp18, tail sheath protein
dV: gp18, tail sheath protein
dW: gp18, tail sheath protein
dZ: gp18, tail sheath protein
eB: gp6, baseplate wedge protein
eU: gp6, baseplate wedge protein
eW: gp6, baseplate wedge protein
f9: gp6, baseplate wedge protein
fA: gp6, baseplate wedge protein
fB: gp6, baseplate wedge protein
fR: gp6, baseplate wedge protein
fS: gp6, baseplate wedge protein
fU: gp6, baseplate wedge protein
fW: gp6, baseplate wedge protein
fr: gp6, baseplate wedge protein
fs: gp6, baseplate wedge protein
gE: gp5, baseplate central spike protein
gG: gp5, baseplate central spike protein
gH: gp5, baseplate central spike protein
gd: gp5.4, tip of the central spike
h1: gp19, tail tube protein
h2: gp19, tail tube protein
hI: gp19, tail tube protein
hJ: gp19, tail tube protein
hK: gp19, tail tube protein
hL: gp19, tail tube protein
hM: gp19, tail tube protein
hX: gp19, tail tube protein
he: gp19, tail tube protein
hf: gp19, tail tube protein
hg: gp19, tail tube protein
hh: gp19, tail tube protein
hi: gp19, tail tube protein
hj: gp19, tail tube protein
hk: gp19, tail tube protein
hl: gp19, tail tube protein
hm: gp19, tail tube protein
hn: gp19, tail tube protein
ho: gp19, tail tube protein
hp: gp19, tail tube protein
hq: gp19, tail tube protein
hr: gp19, tail tube protein
hs: gp19, tail tube protein
ht: gp19, tail tube protein
hu: gp19, tail tube protein
hv: gp19, tail tube protein
hw: gp19, tail tube protein
hx: gp19, tail tube protein
hy: gp19, tail tube protein
hz: gp19, tail tube protein
iC: gp27, baseplate hub protein
iD: gp27, baseplate hub protein
iF: gp27, baseplate hub protein
ja: gp29, tape measure protein
jb: gp29, tape measure protein
jc: gp29, tape measure protein
k3: gp53, baseplate wedge protein
k4: gp53, baseplate wedge protein
k7: gp53, baseplate wedge protein
k8: gp53, baseplate wedge protein
kV: gp53, baseplate wedge protein
lV: gp53, baseplate wedge protein
m1: gp25, baseplate wedge protein
m2: gp25, baseplate wedge protein
m5: gp25, baseplate wedge protein
m6: gp25, baseplate wedge protein
mT: gp25, baseplate wedge protein
nT: gp25, baseplate wedge protein
o0: gp48, baseplate protein
o1: gp48, baseplate protein
o2: gp48, baseplate protein
o6: gp48, baseplate protein
pA: gp48, baseplate protein
pB: gp48, baseplate protein
q3: gp54, baseplate protein
q4: gp54, baseplate protein
q5: gp54, baseplate protein
q7: gp54, baseplate protein
q8: gp54, baseplate protein
q9: gp54, baseplate protein
rc: unassigned peptide A
rd: unassigned peptide A
re: unassigned peptide A
rf: unassigned peptide A
rg: unassigned peptide A
rh: unassigned peptide A
sQ: unassigned peptide B
sT: unassigned peptide B
sU: unassigned peptide B
tS: unassigned peptide C
tX: unassigned peptide C
tY: unassigned peptide C
uR: unassigned peptide D
uV: unassigned peptide D
uW: unassigned peptide D
vZ: unassigned peptide E
va: unassigned peptide E
vb: unassigned peptide E
hetero molecules


Theoretical massNumber of molelcules
Total (without water)9,609,007226
Polymers9,608,615220
Non-polymers3926
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein , 17 types, 202 molecules S0S1S2S3S4S5S6S7S8SASOSPSQSvSwSxSySzT1T2T3T4T5T6T7T8T9TFTGTH...

#1: Protein
gp12, short tail fiber protein / Gene product 12 / gp12


Mass: 56253.926 Da / Num. of mol.: 18 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: P10930
#2: Protein
gp9, baseplate wedge tail fiber connector


Mass: 31024.725 Da / Num. of mol.: 18 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9SWG4
#3: Protein
gp11, baseplate wedge protein


Mass: 23725.523 Da / Num. of mol.: 18 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9SW18
#4: Protein
gp10, baseplate wedge protein / Gene product 10 / gp10


Mass: 66281.680 Da / Num. of mol.: 18 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: P10928
#5: Protein
gp7, baseplate wedge protein / Gene product 7 / gp7


Mass: 119336.516 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: P19061
#6: Protein
gp8, baseplate wedge protein


Mass: 38041.668 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9SVZ9
#7: Protein ...
gp18, tail sheath protein


Mass: 71348.625 Da / Num. of mol.: 36 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9SVW9
#8: Protein
gp6, baseplate wedge protein / Gene product 6 / gp6


Mass: 74492.641 Da / Num. of mol.: 12 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: P19060
#9: Protein gp5, baseplate central spike protein / Pre-Gp5 / Peptidoglycan hydrolase gp5


Mass: 63183.723 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9SVH6, lysozyme
#10: Protein gp5.4, tip of the central spike


Mass: 10233.663 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9SVS3
#11: Protein ...
gp19, tail tube protein


Mass: 18479.613 Da / Num. of mol.: 30 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9SVI2
#12: Protein gp27, baseplate hub protein


Mass: 44431.062 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9XHP4
#13: Protein gp29, tape measure protein / TMP / Gene product 29 / gp29 / Tail length regulator


Mass: 64485.539 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: P13337
#14: Protein
gp53, baseplate wedge protein


Mass: 22990.885 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9XI21
#15: Protein
gp25, baseplate wedge protein


Mass: 15111.101 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9SW44
#16: Protein
gp48, baseplate protein


Mass: 39770.332 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: A0A7S9SVT9
#17: Protein
gp54, baseplate protein / Gene product 54 / gp54


Mass: 35001.188 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus) / References: UniProt: P13341

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Unassigned peptide ... , 5 types, 18 molecules rcrdrerfrgrhsQsTsUtStXtYuRuVuWvZvavb

#18: Protein/peptide
unassigned peptide A


Mass: 2145.636 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus)
#19: Protein/peptide unassigned peptide B


Mass: 1549.902 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus)
#20: Protein/peptide unassigned peptide C


Mass: 869.063 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus)
#21: Protein/peptide unassigned peptide D


Mass: 613.749 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus)
#22: Protein/peptide unassigned peptide E


Mass: 783.958 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Escherichia phage T4 (virus)

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Non-polymers , 1 types, 6 molecules

#23: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Zn

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Escherichia phage T4 / Type: VIRUS / Entity ID: #1-#9 / Source: NATURAL
Source (natural)Organism: Escherichia phage T4 (virus)
Details of virusEmpty: NO / Enveloped: NO / Isolate: STRAIN / Type: VIRION
Natural hostOrganism: Escherichia coli / Strain: B
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid type: PELCO Ultrathin Carbon with Lacey Carbon
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K
Details: Ted Pella lacey carbon 400 mesh grids (catalog No: 01824) were used.

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 600 nm
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingElectron dose: 38 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM softwareName: PHENIX / Version: 1.21.1_5286 / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C3 (3 fold cyclic)
3D reconstructionResolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 113800 / Symmetry type: POINT

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