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- EMDB-48324: Structure of the bacteriophage T4 portal-neck-tail complex -

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Basic information

Entry
Database: EMDB / ID: EMD-48324
TitleStructure of the bacteriophage T4 portal-neck-tail complex
Map dataComposite map of the bacteriophage T4 portal-neck-tail complex
Sample
  • Virus: Escherichia phage T4 (virus)
    • Protein or peptide: x 31 types
  • Ligand: x 1 types
Keywordsbacteriophage T4 / portal vertex / phage neck / phage tail / contractile sheath / tail tube / baseplate / VIRUS / VIRAL PROTEIN
Function / homology
Function and homology information


virus tail, sheath / symbiont genome ejection through host cell envelope, contractile tail mechanism / viral capsid, decoration / symbiont entry into host cell via disruption of host cell wall peptidoglycan / T=13 icosahedral viral capsid / viral genome packaging / virus tail, baseplate / viral tail assembly / virus tail, fiber / symbiont entry into host cell via disruption of host cell envelope ...virus tail, sheath / symbiont genome ejection through host cell envelope, contractile tail mechanism / viral capsid, decoration / symbiont entry into host cell via disruption of host cell wall peptidoglycan / T=13 icosahedral viral capsid / viral genome packaging / virus tail, baseplate / viral tail assembly / virus tail, fiber / symbiont entry into host cell via disruption of host cell envelope / viral release from host cell / peptidoglycan catabolic process / virion component / cell wall macromolecule catabolic process / viral capsid / lysozyme / lysozyme activity / killing of cells of another organism / entry receptor-mediated virion attachment to host cell / defense response to bacterium / symbiont entry into host cell / structural molecule activity / metal ion binding / identical protein binding
Similarity search - Function
Bacteriophage T4, Gp14, neck protein / Virus neck protein / Portal protein Gp20 / Bacteriophage T4-like portal protein (Gp20) / Highly immunogenic outer capsid protein / Bacteriophage T4, Gp12 / Baseplate tail-tube protein gp48, T4-like virus / Short tail fibre protein, C-terminal / Small outer capsid protein / Myoviridae tail sheath stabiliser ...Bacteriophage T4, Gp14, neck protein / Virus neck protein / Portal protein Gp20 / Bacteriophage T4-like portal protein (Gp20) / Highly immunogenic outer capsid protein / Bacteriophage T4, Gp12 / Baseplate tail-tube protein gp48, T4-like virus / Short tail fibre protein, C-terminal / Small outer capsid protein / Myoviridae tail sheath stabiliser / Small outer capsid protein superfamily / Myoviridae tail sheath stabiliser superfamily / Short tail fibre protein, C-terminal superfamily / Phage short tail fibre protein gp12, middle domain / Tail-tube assembly protein / Short tail fibre protein receptor-binding domain / T4-like virus Myoviridae tail sheath stabiliser / Small outer capsid protein / Baseplate wedge protein gp53, bacteriophage T4 / Baseplate wedge protein gp7 / : / : / : / Base plate wedge protein 53 / Baseplate wedge protein gp7, domain V / Baseplate wedge protein gp7, helical domain / Baseplate wedge protein gp7, domain VI / Bacteriophage T4, Gp27, baseplate hub, C-terminal / Bacteriophage T4, Gp27, baseplate hub, N-terminal / Gp27, baseplate hub, domain 3 / Gp27, baseplate hub, domain 4 / Gp27, baseplate hub, domain 2 / Baseplate structural protein, domain 2 / Baseplate structural protein, domain 1 / Baseplate structural protein Gp11 / Bacteriophage T4, Gp11, C-terminal finger domain / Baseplate structural protein Gp11, N-terminal domain superfamily / Baseplate structural protein Gp11 superfamily / Major capsid protein, Myoviridae / Baseplate structural protein Gp11, C-terminal domain / : / GP11 baseplate wedge protein / Tail sheath protein Gp18-like domain I / Major capsid protein Gp23 / Baseplate wedge protein gp10 / Baseplate wedge protein gp6 / Phage tail collar domain superfamily / : / : / : / : / : / : / Baseplate wedge protein gp6-like, helical domain / Baseplate structural protein gp6, C-terminal domain I / Baseplate structural protein gp6, C-terminal domain II / Baseplate wedge protein gp6, domain II / Baseplate structural protein gp6, C-terminal domain III / Baseplate wedge protein gp10 domain 3 / Capsid protein, T4-like bacteriophage-like / Phage tail collar domain / Baseplate structural protein Gp9 C-terminal domain superfamily / Phage Tail Collar Domain / Bacteriophage T4, Gp8 / Bacteriophage T4, Gp8 superfamily / Bacteriophage T4, Gp8 / Gp5, C-terminal / Gp5 C-terminal repeat (3 copies) / : / Baseplate structural protein Gp10, C-terminal domain / Baseplate structural protein Gp9/Gp10 / Baseplate structural protein Gp9/Gp10 middle domain superfamily / Gp9-like superfamily / : / Bacteriophage T4 gp9/10-like protein, N-terminal / Bacteriophage T4 gp9/10-like protein, C-terminal / Protein Gp5, N-terminal OB-fold domain / Pre-baseplate central spike protein Gp5 / Gp5 N-terminal OB domain / : / Tail sheath protein Gp18 domain III N-terminal region / PAAR motif / PAAR motif / IraD/Gp25-like / Phage tail sheath protein, beta-sandwich domain / Baseplate wedge protein gp25 / Phage tail sheath protein beta-sandwich domain / Bacteriophage T4, Gp19, tail tube / T4-like virus tail tube protein gp19 / PKD domain / : / Tail sheath protein, subtilisin-like domain / Phage tail sheath protein subtilisin-like domain / Tail sheath protein, C-terminal domain / Phage tail sheath C-terminal domain / PKD domain / PKD domain superfamily / PKD/Chitinase domain / Repeats in polycystic kidney disease 1 (PKD1) and other proteins / Fibritin C-terminal
Similarity search - Domain/homology
Tail completion and sheath stabilizer protein / Pre-baseplate central spike protein Gp5 / Tail tube protein / Small outer capsid protein / Phospholipase / Baseplate tail tube cap / Tail sheath protein / Baseplate wedge subunit / Highly immunogenic outer capsid protein / Portal protein ...Tail completion and sheath stabilizer protein / Pre-baseplate central spike protein Gp5 / Tail tube protein / Small outer capsid protein / Phospholipase / Baseplate tail tube cap / Tail sheath protein / Baseplate wedge subunit / Highly immunogenic outer capsid protein / Portal protein / Baseplate wedge subunit and tail pin / Baseplate wedge subunit / Baseplate wedge tail fiber connector / Tail sheath stabilizer and completion protein / Baseplate hub subunit / Baseplate wedge subunit / Major capsid protein / Fibritin / Baseplate wedge protein gp10 / Short tail fiber protein gp12 / Neck protein gp13 / Neck protein gp14 / Tape measure protein / Baseplate tail-tube junction protein gp54 / Baseplate wedge protein gp6 / Baseplate wedge protein gp7
Similarity search - Component
Biological speciesEscherichia phage T4 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsFokine A / Zhu J / Klose T / Vago F / Arnaud C / Wang Z / Khare B / Rossmann MG / Chen Z / Sun L ...Fokine A / Zhu J / Klose T / Vago F / Arnaud C / Wang Z / Khare B / Rossmann MG / Chen Z / Sun L / Fang Q / Kuhn R / Rao VB
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI081726 United States
CitationJournal: To Be Published
Title: In situ Atomic Structure of Phage T4 Head-Tail Connector Complex Shows Molecular Gates Open for Genome Ejection and DNA Pushed into the Tail Tube
Authors: Fokine A / Zhu J / Klose T / Vago F / Arnaud C / Wang Z / Khare B / Rossmann MG / Chen Z / Sun L / Fang Q / Kuhn R / Rao VB
History
DepositionDec 17, 2024-
Header (metadata) releaseDec 24, 2025-
Map releaseDec 24, 2025-
UpdateDec 24, 2025-
Current statusDec 24, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_48324.png

Downloads & links

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Map

FileDownload / File: emd_48324.map.gz / Format: CCP4 / Size: 652.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite map of the bacteriophage T4 portal-neck-tail complex
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.62 Å/pix.
x 1030 pix.
= 1664.48 Å		1.62 Å/pix.
x 407 pix.
= 657.712 Å		1.62 Å/pix.
x 408 pix.
= 659.328 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 1.616 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.012
Minimum - Maximum0.0 - 0.0963556
Average (Standard dev.)0.0012228369 (±0.0033948196)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-4-5-295
Dimensions4074081030
Spacing4084071030
CellA: 659.328 Å / B: 657.71204 Å / C: 1664.4801 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Escherichia phage T4

EntireName: Escherichia phage T4 (virus)
Components
  • Virus: Escherichia phage T4 (virus)
    • Protein or peptide: gp14, neck protein
    • Protein or peptide: gp18, tail sheath protein
    • Protein or peptide: gp19, tail tube protein
    • Protein or peptide: gpwac, fibritin
    • Protein or peptide: gp20, portal protein
    • Protein or peptide: gp23, major capsid protein
    • Protein or peptide: gp13, neck protein
    • Protein or peptide: gp15, tail terminator protein
    • Protein or peptide: gp3, tube terminator protein
    • Protein or peptide: Hoc, highly immunogenic outer capsid protein
    • Protein or peptide: Soc, small outer capsid protein
    • Protein or peptide: gp12, short tail fiber protein
    • Protein or peptide: gp9, baseplate wedge tail fiber connector
    • Protein or peptide: gp11, baseplate wedge protein
    • Protein or peptide: gp10, baseplate wedge protein
    • Protein or peptide: gp7, baseplate wedge protein
    • Protein or peptide: gp8, baseplate wedge protein
    • Protein or peptide: gp6, baseplate wedge protein
    • Protein or peptide: gp5, baseplate central spike protein
    • Protein or peptide: gp5.4, tip of the central spike
    • Protein or peptide: gp27, baseplate hub protein
    • Protein or peptide: gp29, tape measure protein
    • Protein or peptide: gp53, baseplate wedge protein
    • Protein or peptide: gp25, baseplate protein
    • Protein or peptide: gp48, baseplate protein
    • Protein or peptide: gp54, baseplate protein
    • Protein or peptide: unassigned peptide A
    • Protein or peptide: unassigned peptide B
    • Protein or peptide: unassigned peptide C
    • Protein or peptide: unassigned peptide D
    • Protein or peptide: unassigned peptide E
  • Ligand: ZINC ION

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Supramolecule #1: Escherichia phage T4

SupramoleculeName: Escherichia phage T4 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1-#31 / NCBI-ID: 2681598 / Sci species name: Escherichia phage T4 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Escherichia coli (E. coli) / Strain: B

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Macromolecule #1: gp14, neck protein

MacromoleculeName: gp14, neck protein / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 29.597836 KDa
SequenceString: MATYDKNLFA KLENRTGYSQ TNETEILNPY VNFNHYKNSQ ILADVLVAES IQMRGVECYY VPREYVSPDL IFGEDLKNKF TKAWKFAAY LNSFEGYEGA KSFFSNFGMQ VQDEVTLSIN PNLFKHQVNG KEPKEGDLIY FPMDNSLFEI NWVEPYDPFY Q LGQNAIRK ...String:
MATYDKNLFA KLENRTGYSQ TNETEILNPY VNFNHYKNSQ ILADVLVAES IQMRGVECYY VPREYVSPDL IFGEDLKNKF TKAWKFAAY LNSFEGYEGA KSFFSNFGMQ VQDEVTLSIN PNLFKHQVNG KEPKEGDLIY FPMDNSLFEI NWVEPYDPFY Q LGQNAIRK ITAGKFIYSG EEINPVLQKN EGINIPEFSE LELNAVRNLN GIHDINIDQY AEVDQINSEA KEYVEPYVVV NN RGKSFES SPFDNDFMD

UniProtKB: Neck protein gp14

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Macromolecule #2: gp18, tail sheath protein

MacromoleculeName: gp18, tail sheath protein / type: protein_or_peptide / ID: 2 / Number of copies: 138 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 71.348625 KDa
SequenceString: MTLLSPGIEL KETTVQSTVV NNSTGTAALA GKFQWGPAFQ IKQVTNEVDL VNTFGQPTAE TADYFMSAMN FLQYGNDLRV VRAVDRDTA KNSSPIAGNI EYTISTPGSN YAVGDKITVK YVSDDIETEG KITEVDADGK IKKINIPTAK IIAKAKEVGE Y PTLGSNWT ...String:
MTLLSPGIEL KETTVQSTVV NNSTGTAALA GKFQWGPAFQ IKQVTNEVDL VNTFGQPTAE TADYFMSAMN FLQYGNDLRV VRAVDRDTA KNSSPIAGNI EYTISTPGSN YAVGDKITVK YVSDDIETEG KITEVDADGK IKKINIPTAK IIAKAKEVGE Y PTLGSNWT AEISSSSSGL AAVITLGKII TDSGILLAEI ENAEAAMTAV DFQANLKKYG IPGVVALYPG ELGDKIEIEI VS KADYAKG ASALLPIYPG GGTRASTAKA VFGYGPQTDS QYAIIVRRND AIVQSVVLST KRGGKDIYDS NIYIDDFFAK GGS EYIFAT AQNWPEGFSG ILTLSGGLSS NAEVTAGDLM EAWDFFADRE SVDVQLFIAG SCAGESLETA STVQKHVVSI GDVR QDCLV LCSPPRETVV GIPVTRAVDN LVNWRTAAGS YTDNNFNISS TYAAIDGNYK YQYDKYNDVN RWVPLAADIA GLCAR TDNV SQTWMSPAGY NRGQILNVIK LAIETRQAQR DRLYQEAINP VTGTGGDGYV LYGDKTATSV PSPFDRINVR RLFNML KTN IGRSSKYRLF ELNNAFTRSS FRTETAQYLQ GIKALGGIYE YRVVCDTTNN TPSVIDRNEF VATFYIQPAR SINYITL NF VATATGADFD ELTGLAG

UniProtKB: Tail sheath protein

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Macromolecule #3: gp19, tail tube protein

MacromoleculeName: gp19, tail tube protein / type: protein_or_peptide / ID: 3 / Number of copies: 126 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 18.479613 KDa
SequenceString:
MFVDDVTRAF ESGDFARPNL FQVEISYLGQ NFTFQCKATA LPAGIVEKIP VGFMNRKINV AGDRTFDDWT VTVMNDEAHD ARQKFVDWQ SIAAGQGNEI TGGKPAEYKK SAIVRQYARD AKTVTKEIEI KGLWPTNVGE LQLDWDSNNE IQTFEVTLAL D YWE

UniProtKB: Tail tube protein

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Macromolecule #4: gpwac, fibritin

MacromoleculeName: gpwac, fibritin / type: protein_or_peptide / ID: 4 / Number of copies: 36 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 51.909164 KDa
SequenceString: MTDIVLNDLP FVDGPPAEGQ SRISWIKNGE EILGADTQYG SEGSMNRPTV SVLRNVEVLD KNIGILKTSL ETANSDIKTI QGILDVSGD IEALAQIGIN KKDISDLKTL TSEHTEILNG TNNTVDSILA DIGPFNAEAN SVYRTIRNDL LWIKRELGQY T GQDINGLP ...String:
MTDIVLNDLP FVDGPPAEGQ SRISWIKNGE EILGADTQYG SEGSMNRPTV SVLRNVEVLD KNIGILKTSL ETANSDIKTI QGILDVSGD IEALAQIGIN KKDISDLKTL TSEHTEILNG TNNTVDSILA DIGPFNAEAN SVYRTIRNDL LWIKRELGQY T GQDINGLP VVGNPSSGMK HRIINNTDVI TSQGIRLSEL ETKFIESDVG SLTIEVGNLR EELGPKPPSF SQNVYSRLNE ID TKQTTVE SDISAIKTSI GYPGNNSIIT SVNTNTDNIA SINLELNQSG GIKQRLTVIE TSIGSDDIPS SIKGQIKDNT TSI ESLNGI VGENTSSGLR ANVSWLNQIV GTDSSGGQPS PPGSLLNRVS TIETSVSGLN NAVQNLQVEI GNNSAGIKGQ VVAL NTLVN GTNPNGSTVE ERGLTNSIKA NETNIASVTQ EVNTAKGNIS SLQGDVQALQ EAGYIPEAPR DGQAYVRKDG EWVFL STFL SPA

UniProtKB: Fibritin

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Macromolecule #5: gp20, portal protein

MacromoleculeName: gp20, portal protein / type: protein_or_peptide / ID: 5 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 61.117082 KDa
SequenceString: MKFNVLSLFA PWAKMDERNF KDQEKEDLVS ITAPKLDDGA REFEVSSNEA ASPYNAAFQT IFGSYEPGMK TTRELIDTYR NLMNNYEVD NAVSEIVSDA IVYEDDTEVV ALNLDKSKFS PKIKNMMLDE FSDVLNHLSF QRKGSDHFRR WYVDSRIFFH K IIDPKRPK ...String:
MKFNVLSLFA PWAKMDERNF KDQEKEDLVS ITAPKLDDGA REFEVSSNEA ASPYNAAFQT IFGSYEPGMK TTRELIDTYR NLMNNYEVD NAVSEIVSDA IVYEDDTEVV ALNLDKSKFS PKIKNMMLDE FSDVLNHLSF QRKGSDHFRR WYVDSRIFFH K IIDPKRPK EGIKELRRLD PRQVQYVREI ITETEAGTKI VKGYKEYFIY DTAHESYACD GRMYEAGTKI KIPKAAVVYA HS GLVDCCG KNIIGYLHRA VKPANQLKLL EDAVVIYRIT RAPDRRVWYV DTGNMPARKA AEHMQHVMNT MKNRVVYDAS TGK IKNQQH NMSMTEDYWL QRRDGKAVTE VDTLPGADNT GNMEDIRWFR QALYMALRVP LSRIPQDQQG GVMFDSGTSI TRDE LTFAK FIRELQHKFE EVFLDPLKTN LLLKGIITED EWNDEINNIK IEFHRDSYFA ELKEAEILER RINMLTMAEP FIGKY ISHR TAMKDILQMT DEEIEQEAKQ IEEESKEARF QDPDQEQEDF

UniProtKB: Portal protein

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Macromolecule #6: gp23, major capsid protein

MacromoleculeName: gp23, major capsid protein / type: protein_or_peptide / ID: 6 / Number of copies: 30 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 56.074242 KDa
SequenceString: MTIKTKAELL NKWKPLLEGE GLPEIANSKQ AIIAKIFENQ EKDFQTAPEY KDEKIAQAFG SFLTEAEIGG DHGYNATNIA AGQTSGAVT QIGPAVMGMV RRAIPNLIAF DICGVQPMNS PTGQVFALRA VYGKDPVAAG AKEAFHPMYG PDAMFSGQGA A KKFPALAA ...String:
MTIKTKAELL NKWKPLLEGE GLPEIANSKQ AIIAKIFENQ EKDFQTAPEY KDEKIAQAFG SFLTEAEIGG DHGYNATNIA AGQTSGAVT QIGPAVMGMV RRAIPNLIAF DICGVQPMNS PTGQVFALRA VYGKDPVAAG AKEAFHPMYG PDAMFSGQGA A KKFPALAA STQTTVGDIY THFFQETGTV YLQASVQVTI DAGATDAAKL DAEIKKQMEA GALVEIAEGM ATSIAELQEG FN GSTDNPW NEMGFRIDKQ VIEAKSRQLK AAYSIELAQD LRAVHGMDAD AELSGILATE IMLEINREVV DWINYSAQVG KSG MTLTPG SKAGVFDFQD PIDIRGARWA GESFKALLFQ IDKEAVEIAR QTGRGEGNFI IASRNVVNVL ASVDTGISYA AQGL ATGFS TDTTKSVFAG VLGGKYRVYI DQYAKQDYFT VGYKGPNEMD AGIYYAPYVA LTPLRGSDPK NFQPVMGFKT RYGIG INPF AESAAQAPAS RIQSGMPSIL NSLGKNAYFR RVYVKGI

UniProtKB: Major capsid protein

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Macromolecule #7: gp13, neck protein

MacromoleculeName: gp13, neck protein / type: protein_or_peptide / ID: 7 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 34.772211 KDa
SequenceString: MSGYNPQNPK ELKDVILRRL GAPIINVELT PDQIYDCIQR ALELYGEYHF DGLNKGFHVF YVGDDEERYK TGVFDLRGSN VFAVTRILR TNIGSITSMD GNATYPWFTD FLLGMAGING GMGTSCNRFY GPNAFGADLG YFTQLTSYMG MMQDMLSPIP D FWFNSANE ...String:
MSGYNPQNPK ELKDVILRRL GAPIINVELT PDQIYDCIQR ALELYGEYHF DGLNKGFHVF YVGDDEERYK TGVFDLRGSN VFAVTRILR TNIGSITSMD GNATYPWFTD FLLGMAGING GMGTSCNRFY GPNAFGADLG YFTQLTSYMG MMQDMLSPIP D FWFNSANE QLKVMGNFQK YDLIIVESWT KSYIDTNKMV GNTVGYGTVG PQDSWSLSER YNNPDHNLVG RVVGQDPNVK QG AYNNRWV KDYATALAKE LNGQILARHQ GMMLPGGVTI DGQRLIEEAR LEKEALREEL YLLDPPFGIL VG

UniProtKB: Neck protein gp13

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Macromolecule #8: gp15, tail terminator protein

MacromoleculeName: gp15, tail terminator protein / type: protein_or_peptide / ID: 8 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 31.587486 KDa
SequenceString: MFGYFYNSSF RRYATLMGDL FSNIQIKRQL ESGDKFIRVP ITYASKEHFM MKLNKWTSIN SQEDVAKVET ILPRINLHLV DFSYNAPFK TNILNQNLLQ KGATSVVSQY NPSPIKMIYE LSIFTRYEDD MFQIVEQILP YFQPHFNTTM YEQFGNDIPF K RDIKIVLM ...String:
MFGYFYNSSF RRYATLMGDL FSNIQIKRQL ESGDKFIRVP ITYASKEHFM MKLNKWTSIN SQEDVAKVET ILPRINLHLV DFSYNAPFK TNILNQNLLQ KGATSVVSQY NPSPIKMIYE LSIFTRYEDD MFQIVEQILP YFQPHFNTTM YEQFGNDIPF K RDIKIVLM SAAIDEAIDG DNLSRRRIEW SLTFEVNGWM YPPVDDAEGL IRTTYTDFHA NTRDLPDGEG VFESVDSEVV PR DIDPEDW DGTVKQTFTS NVNRPTPPEP PGPRT

UniProtKB: Tail sheath stabilizer and completion protein

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Macromolecule #9: gp3, tube terminator protein

MacromoleculeName: gp3, tube terminator protein / type: protein_or_peptide / ID: 9 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 19.723143 KDa
SequenceString:
MSQALQQIFN QANTTNFVVS IPHSNTTSAF TLNAQSVPIP GIRIPVTDTV TGPFGLGRAQ RPGVTFEYDP LIVRFIVDEE LKSWIGMYE WMLGTSNYLT GENTAQKTGP EYITLYILDN SKTEIVMSIN FYKPWVSDLS EVEFSYTEDS DPALVCTATI P YTYFQVEK DGKIIAEV

UniProtKB: Tail completion and sheath stabilizer protein

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Macromolecule #10: Hoc, highly immunogenic outer capsid protein

MacromoleculeName: Hoc, highly immunogenic outer capsid protein / type: protein_or_peptide / ID: 10 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 40.416547 KDa
SequenceString: MTFTVDITPK TPTGVIDETK QFTATPSGQT GGGTITYAWS VDNVPQDGAE ATFSYVLKGP AGQKTIKVVA TNTLSEGGPE TAEATTTIT VKNKTQTTTL AVTPASPAAG VIGTPVQFTA ALASQPDGAS ATYQWYVDDS QVGGETNSTF SYTPTTSGVK R IKCVAQVT ...String:
MTFTVDITPK TPTGVIDETK QFTATPSGQT GGGTITYAWS VDNVPQDGAE ATFSYVLKGP AGQKTIKVVA TNTLSEGGPE TAEATTTIT VKNKTQTTTL AVTPASPAAG VIGTPVQFTA ALASQPDGAS ATYQWYVDDS QVGGETNSTF SYTPTTSGVK R IKCVAQVT ATDYDALSVT SNEVSLTVNK KTMNPQVTLT PPSINVQQDA SATFTANVTG APEEAQITYS WKKDSSPVEG ST NVYTVDT SSVGSQTIEV TATVTAADYN PVTVTKTGNV TVTAKVAPEP EGELPYVHPL PHRSSAYIWC GWWVMDEIQK MTE EGKDWK TDDPDSKYYL HRYTLQKMMK DYPEVDVQES RNGYIIHKTA LETGIIYTYP

UniProtKB: Highly immunogenic outer capsid protein

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Macromolecule #11: Soc, small outer capsid protein

MacromoleculeName: Soc, small outer capsid protein / type: protein_or_peptide / ID: 11 / Number of copies: 10 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 9.129147 KDa
SequenceString:
MASTRGYVNI KTFEQKLDGN KKIEGKEISV AFPLYSDVHK ISGAHYQTFP SEKAAYSTVY EENQRTEWIA ANEDLWKVTG

UniProtKB: Small outer capsid protein

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Macromolecule #12: gp12, short tail fiber protein

MacromoleculeName: gp12, short tail fiber protein / type: protein_or_peptide / ID: 12 / Number of copies: 18 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 56.253926 KDa
SequenceString: MSNNTYQHVS NESRYVKFDP TDTNFPPEIT DVQAAIAAIS PAGVNGVPDA SSTTKGILFI PTEQEVIDGT NNTKAVTPAT LATRLSYPN ATETVYGLTR YSTNDEAIAG VNNESSITPA KFTVALNNAF ETRVSTESSN GVIKISSLPQ ALAGADDTTA M TPLKTQQL ...String:
MSNNTYQHVS NESRYVKFDP TDTNFPPEIT DVQAAIAAIS PAGVNGVPDA SSTTKGILFI PTEQEVIDGT NNTKAVTPAT LATRLSYPN ATETVYGLTR YSTNDEAIAG VNNESSITPA KFTVALNNAF ETRVSTESSN GVIKISSLPQ ALAGADDTTA M TPLKTQQL AIKLIAQIAP SETTATESDQ GVVQLATVAQ VRQGTLREGY AISPYTFMNS SSTEEYKGVI KLGTQSEVNS NN ASVAVTG ATLNGRGSTT SMRGVVKLTT TAGSQSGGDA SSALAWNADV IQQRGGQIIY GTLRIEDTFT IANGGANITG TVR MTGGYI QGNRIVTQNE IDRTIPVGAI MMWAADSLPS DAWRFCHGGT VSASDCPLYA SRIGTRYGGN PSNPGLPDMR GLFV RGSGR GSHLTNPNVN GNDQFGKPRL GVGCTGGYVG EVQIQQMSYH KHAGGFGEHD DLGAFGNTRR SNFVGTRKGL DWDNR SYFT NDGYEIDPES QRNSKYTLNR PELIGNETRP WNISLNYIIK VKE

UniProtKB: Short tail fiber protein gp12

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Macromolecule #13: gp9, baseplate wedge tail fiber connector

MacromoleculeName: gp9, baseplate wedge tail fiber connector / type: protein_or_peptide / ID: 13 / Number of copies: 18 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 31.024725 KDa
SequenceString: MFIQEPKKLI DTGEIGNAST GDILFDGGNK INSDFNAIYN AFGDQRKMAV ANGTGADGQI IHATGYYQKH SITEYATPVK VGTRHDIDT STVGVKVIIE RGELGDCVEF INSNGSISVT NPLTIQAIDS IKGVSGNLVV TSPYSKVTLR CISSDNSTSV W NYSIESMF ...String:
MFIQEPKKLI DTGEIGNAST GDILFDGGNK INSDFNAIYN AFGDQRKMAV ANGTGADGQI IHATGYYQKH SITEYATPVK VGTRHDIDT STVGVKVIIE RGELGDCVEF INSNGSISVT NPLTIQAIDS IKGVSGNLVV TSPYSKVTLR CISSDNSTSV W NYSIESMF GQKESPAEGT WNISTSGSVD IPLFHRTEYN MAKLLVTCQS VDGRKIKTAE INILVDTVNS EVISSEYAVM RV GNETEED EIANIAFSIK ENYVTATISS STVGMRAAVK VIATQKIGVA Q

UniProtKB: Baseplate wedge tail fiber connector

+
Macromolecule #14: gp11, baseplate wedge protein

MacromoleculeName: gp11, baseplate wedge protein / type: protein_or_peptide / ID: 14 / Number of copies: 18 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 23.725523 KDa
SequenceString: MSLLNNKAGV ISRLADFLGF RPKTGDIDVM NRQSVGSVTI SQLAKGFYEP NIESAINDVH NFSIKDVGTI ITNKTGVSPE GVSQTDYWA FSGTVTDDSL PPGSPITVLV FGLPVSATTG MTAIEFVAKV RVALQEAIAS FTAINSYKDH PTDGSKLEVT Y LDNQKHVL ...String:
MSLLNNKAGV ISRLADFLGF RPKTGDIDVM NRQSVGSVTI SQLAKGFYEP NIESAINDVH NFSIKDVGTI ITNKTGVSPE GVSQTDYWA FSGTVTDDSL PPGSPITVLV FGLPVSATTG MTAIEFVAKV RVALQEAIAS FTAINSYKDH PTDGSKLEVT Y LDNQKHVL STYSTYGITI SQEIISESKP GYGTWNLLGA QTVTLDNQQT PTVFYHFERT A

UniProtKB: Baseplate wedge subunit and tail pin

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Macromolecule #15: gp10, baseplate wedge protein

MacromoleculeName: gp10, baseplate wedge protein / type: protein_or_peptide / ID: 15 / Number of copies: 18 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 66.28168 KDa
SequenceString: MKQNINIGNV VDDGTGDYLR KGGIKINENF DELYYELGDG DVPYSAGAWK TYNASSGQTL TAEWGKSYAI NTSSGRVTIN LPKGTVNDY NKVIRARDVF ATWNVNPVTL VAASGDTIKG SAVPVEINVR FSDLELVYCA PGRWEYVKNK QIDKITSSDI S NVARKEFL ...String:
MKQNINIGNV VDDGTGDYLR KGGIKINENF DELYYELGDG DVPYSAGAWK TYNASSGQTL TAEWGKSYAI NTSSGRVTIN LPKGTVNDY NKVIRARDVF ATWNVNPVTL VAASGDTIKG SAVPVEINVR FSDLELVYCA PGRWEYVKNK QIDKITSSDI S NVARKEFL VEVQGQTDFL DVFRGTSYNV NNIRVKHRGN ELYYGDVFSE NSDFGSPGEN EGELVPLDGF NIRLRQPCNI GD TVQIETF MDGVSQWRSS YTRRQIRLLD SKLTSKTSLE GSIYVTDLST MKSIPFSAFG LIPGEPINPN SLEVRFNGIL QEL AGTVGM PLFHCVGADS DDEVECSVLG GTWEQSHTDY SVETDENGIP EILHFDSVFE HGDIINITWF NNDLGTLLTK DEII DETDN LYVSQGPGVD ISGDVNLTDF DKIGWPNVEA VQSYQRAFNA VSNIFDTIYP IGTIYENAVN PNNPVTYMGF GSWKL FGQG KVLVGWNEDI SDPNFALNNN DLDSGGNPSH TAGGTGGSTS VTLENANLPA TETDEEVLIV DENGSVIVGG CQYDPD ESG PIYTKYREAK ASTNSTHTPP TSITNIQPYI TVYRWIRIA

UniProtKB: Baseplate wedge protein gp10

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Macromolecule #16: gp7, baseplate wedge protein

MacromoleculeName: gp7, baseplate wedge protein / type: protein_or_peptide / ID: 16 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 119.336516 KDa
SequenceString: MTVKAPSVTS LRISKLSANQ VQVRWDDVGA NFYYFVEIAE TKTNSGENLP SNQYRWINLG YTANNSFFFD DADPLTTYII RVATAAQDF EQSDWIYTEE FETFATNAYT FQNMIEMQLA NKFIQEKFTL NNSDYVNFNN DTIMAALMNE SFQFSPSYVD V SSISNFII ...String:
MTVKAPSVTS LRISKLSANQ VQVRWDDVGA NFYYFVEIAE TKTNSGENLP SNQYRWINLG YTANNSFFFD DADPLTTYII RVATAAQDF EQSDWIYTEE FETFATNAYT FQNMIEMQLA NKFIQEKFTL NNSDYVNFNN DTIMAALMNE SFQFSPSYVD V SSISNFII GENEYHEIQG SIQQVCKDIN RVYLMESEGI LYLFERYQPV VKVSNDKGQT WKAVKLFNDR VGYPLSKTVY YQ SANTTYV LGYDKIFYGR KSTDVRWSAD DVRFSSQDIT FAKLGDQLHL GFDVEIFATY ATLPANVYRI AEAITCTDDY IYV VARDKV RYIKTSNALI DFDPLSPTYS ERLFEPDTMT ITGNPKAVCY KMDSICDKVF ALIIGEVETL NANPRTSKII DSAD KGIYV LNHDEKTWKR VFGNTEEERR RIQPGYANMS TDGKLVSLSS SNFKFLSDNV VNDPETAAKY QLIGAVKYEF PREWL ADKH YHMMAFIADE TSDWETFTPQ PMKYYAEPFF NWSKKSNTRC WINNSDRAVV VYADLKYTKV IENIPETSPD RLVHEY WDD GDCTIVMPNV KFTGFKKYAS GMLFYKASGE IISYYDFNYR VRDTVEIIWK PTEVFLKAFL QNQEHETPWS PEEERGL AD PDLRPLIGTM MPDSYLLQDS NFEAFCEAYI QYLSDGYGTQ YNNLRNLIRN QYPREEHAWE YLWSEIYKRN IYLNADKR D AVARFFESRS YDFYSTKGIE ASYKFLFKVL YNEEVEIEIE SGAGTEYDII VQSDSLTEDL VGQTIYTATG RCNVTYIER SYSNGKLQWT VTIHNLLGRL IAGQEVKAER LPSFEGEIIR GVKGKDLLQN NIDYINRSRS YYVMKIKSNL PSSRWKSDVI RFVHPVGFG FIAITLLTMF INVGLTLKHT ETIINKYKNY KWDSGLPTEY ADRIAKLTPT GEIEHDSVTG EAIYEPGPMA G VKYPLPDD YNAENNNSIF QGQLPSERRK LMSPLFDASG TTFAQFRDLV NKRLKDNIGN PRDPENPTQV KIDE

UniProtKB: Baseplate wedge protein gp7

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Macromolecule #17: gp8, baseplate wedge protein

MacromoleculeName: gp8, baseplate wedge protein / type: protein_or_peptide / ID: 17 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 38.041668 KDa
SequenceString: MNDSSVIYRA IVTSKFRTEK MLNFYNSIGS GPDKNTIFIT FGRSEPWSSN ENEVGFAPPY PTDSVLGVTD MWTHMMGTVK VLPSMLDAV IPRRDWGDTR YPDPYTFRIN DIVVCNSAPY NATESGAGWL VYRCLDVPDT GMCSIASLTD KDECLKLGGK W TPSARSMT ...String:
MNDSSVIYRA IVTSKFRTEK MLNFYNSIGS GPDKNTIFIT FGRSEPWSSN ENEVGFAPPY PTDSVLGVTD MWTHMMGTVK VLPSMLDAV IPRRDWGDTR YPDPYTFRIN DIVVCNSAPY NATESGAGWL VYRCLDVPDT GMCSIASLTD KDECLKLGGK W TPSARSMT PPEGRGDAEG TIEPGDGYVW EYLFEIPPDV SINRCTNEYI VVPWPEELKE DPTRWGYEDN LTWQQDDFGL IY RVKANTI RFKAYLDSVY FPEAALPGNK GFRQISIITN PLEAKAHPND PNVKAEKDYY DPEDLMRHSG EMIYMENRPP IIM AMDQTE EINILFTF

UniProtKB: Baseplate wedge subunit

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Macromolecule #18: gp6, baseplate wedge protein

MacromoleculeName: gp6, baseplate wedge protein / type: protein_or_peptide / ID: 18 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 74.492641 KDa
SequenceString: MANTPVNYQL TRTANAIPEI FVGGTFAEIK QNLIEWLNGQ NEFLDYDFEG SRLNVLCDLL AYNTLYIQQF GNAAVYESFM RTANLRSSV VQAAQDNGYL PTSKSAAQTE IMLTCTDALN RNYITIPRGT RFLAYAKDTS VNPYNFVSRE DVIAIRDKNN Q YFPRLKLA ...String:
MANTPVNYQL TRTANAIPEI FVGGTFAEIK QNLIEWLNGQ NEFLDYDFEG SRLNVLCDLL AYNTLYIQQF GNAAVYESFM RTANLRSSV VQAAQDNGYL PTSKSAAQTE IMLTCTDALN RNYITIPRGT RFLAYAKDTS VNPYNFVSRE DVIAIRDKNN Q YFPRLKLA QGRIVRTEII YDKLTPIIIY DKNIDRNQVK LYVDGAEWIN WTRKSMVHAG STSTIYYMRE TIDGNTEFYF GE GEISVNA SEGALTANYI GGLKPTQNST IVIEYISTNG ADANGAVGFS YADTLTNITV ININENPNDD PDFVGADGGG DPE DIERIR ELGTIKRETQ QRCVTATDYD TFVSERFGSI IQAVQTFTDS TKPGYAFIAA KPKSGLYLTT VQREDIKNYL KDYN LAPIT PSIISPNYLF IKTNLKVTYA LNKLQESEQW LEGQIIDKID RYYTEDVEIF NSSFAKSKML TYVDDADHSV IGSSA TIQM VREVQNFYKT PEAGIKYNNQ IKDRSMESNT FSFNSGRKVV NPDTGLEEDV LYDVRIVSTD RDSKGIGKVI IGPFAS GDV TENENIQPYT GNDFNKLANS DGRDKYYVIG EINYPADVIY WNIAKINLTS EKFEVQTIEL YSDPTDDVIF TRDGSLI VF ENDLRPQYLT IDLEPISQ

UniProtKB: Baseplate wedge protein gp6

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Macromolecule #19: gp5, baseplate central spike protein

MacromoleculeName: gp5, baseplate central spike protein / type: protein_or_peptide / ID: 19 / Number of copies: 3 / Enantiomer: LEVO / EC number: lysozyme
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 63.183723 KDa
SequenceString: MEMISNNLNW FVGVVEDRMD PLKLGRVRVR VVGLHPPQRA QGDVMGIPTE KLPWMSVIQP ITSAAMSGIG GSVTGPVEGT RVYGHFLDK WKTNGIVLGT YGGIVREKPN RLEGFSDPTG QYPRRLGNDT NVLNQGGEVG YDSSSNVIQD SNLDTAINPD D RPLSEIPT ...String:
MEMISNNLNW FVGVVEDRMD PLKLGRVRVR VVGLHPPQRA QGDVMGIPTE KLPWMSVIQP ITSAAMSGIG GSVTGPVEGT RVYGHFLDK WKTNGIVLGT YGGIVREKPN RLEGFSDPTG QYPRRLGNDT NVLNQGGEVG YDSSSNVIQD SNLDTAINPD D RPLSEIPT DDNPNMSMAE MLRRDEGLRL KVYWDTEGYP TIGIGHLIMK QPVRDMAQIN KVLSKQVGRE ITGNPGSITM EE ATTLFER DLADMQRDIK SHSKVGPVWQ AVNRSRQMAL ENMAFQMGVG GVAKFNTMLT AMLAGDWEKA YKAGRDSLWY QQT KGRASR VTMIILTGNL ESYGVEVKTP ARSLSAMAAT VAKSSDPADP PIPNDSRILF KEPVSSYKGE YPYVHTMETE SGHI QEFDD TPGQERYRLV HPTGTYEEVS PSGRRTRKTV DNLYDITNAD GNFLVAGDKK TNVGGSEIYY NMDNRLHQID GSNTI FVRG DETKTVEGNG TILVKGNVTI IVEGNADITV KGDATTLVEG NQTNTVNGNL SWKVAGTVDW DVGGDWTEKM ASMSSI SSG QYTIDGSRID IG

UniProtKB: Pre-baseplate central spike protein Gp5

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Macromolecule #20: gp5.4, tip of the central spike

MacromoleculeName: gp5.4, tip of the central spike / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 10.233663 KDa
SequenceString:
MSGLSYDKCV TAGHEAWPPT VVNATQSKVF TGGIAVLVAG DPITEHTEIK KPYETHGGVT QPRTSKVYVT GKKAVQMADP ISCGDTVAQ ASSKVFIK

UniProtKB: Phospholipase

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Macromolecule #21: gp27, baseplate hub protein

MacromoleculeName: gp27, baseplate hub protein / type: protein_or_peptide / ID: 21 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 44.431062 KDa
SequenceString: MSMLQRPGYP NLSVKLFDSY DAWSNNRFVE LAATITTLTM RDSLYGRNEG MLQFYDSKNI HTKMDGNEII QISVANANDI NNVKTRIYG CKHFSVSVDS KGDNIIAIEL GTIHSIENLK FGRPFFPDAG ESIKEMLGVI YQDRTLLTPA INAINAYVPD I PWTSTFEN ...String:
MSMLQRPGYP NLSVKLFDSY DAWSNNRFVE LAATITTLTM RDSLYGRNEG MLQFYDSKNI HTKMDGNEII QISVANANDI NNVKTRIYG CKHFSVSVDS KGDNIIAIEL GTIHSIENLK FGRPFFPDAG ESIKEMLGVI YQDRTLLTPA INAINAYVPD I PWTSTFEN YLSYVREVAL AVGSDKFVFV WQDIMGVNMM DYDMMINQEP YPMIVGEPSL IGQFIQELKY PLAYDFVWLT KS NPHKRDP MKNATIYAHS FLDSSIPMIT TGKGENSIVV SRSGAYSEMT YRNGYEEAIR LQTMAQYDGY AKCSTIGNFN LTP GVKIIF NDSKNQFKTE FYVDEVIHEL SNNNSVTHLY MFTNATKLET IDPVKVKNEF KSDTTTEESS SSNKQ

UniProtKB: Baseplate hub subunit

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Macromolecule #22: gp29, tape measure protein

MacromoleculeName: gp29, tape measure protein / type: protein_or_peptide / ID: 22 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 64.485539 KDa
SequenceString: MKKPQEMQTM RRKVISDNKP TQEAAKSASN TLSGLNDIST KLDDAQAASE LIAQTVEEKS NEIIGAIDNV ESAVSDTSAG SELIAETVE IGNNINKEIG ESLGSKLDKL TSLLEQKIQT AGIQQTGTSL ATVESAIPVK VVEDDTAESV GPLLPAPEAV N NDPDADFF ...String:
MKKPQEMQTM RRKVISDNKP TQEAAKSASN TLSGLNDIST KLDDAQAASE LIAQTVEEKS NEIIGAIDNV ESAVSDTSAG SELIAETVE IGNNINKEIG ESLGSKLDKL TSLLEQKIQT AGIQQTGTSL ATVESAIPVK VVEDDTAESV GPLLPAPEAV N NDPDADFF PTPQPVEPKQ ESPEEKQKKE AFNLKLSQAL DKLTKTVDFG FKKSISITDK ISSMLFKYTV SAAIEAAKMT AM ILAVVVG IDLLMIHFKY WSDKFSKAWD LFSTDFTKFS SETGTWGPLL QSIFDSIDKI KQLWEAGDWG GLTVAIVEGL GKV LFNLGE LIQLGMAKLS AAILRVIPGM KDTADEVEGR ALENFQNSTG ASLNKEDQEK VANYQDKRMN GDLGPIAEGL DKIS NWKTR ASNWIRGVDN KEALTTDEER AAEEEKLKQL SPEERKNALM KANEARAAMI RFEKYADSAD MSKDSTVKSV EAAYE DLKK RMDDPDLNNS PAVKKELAAR FSKIDATYQE LKKNQPNAKP ETSAKSPEAK QVQVIEKNKA QQAPVQQASP SINNTN NVI KKNTVVHNMT PVTSTTAPGV FDATGVN

UniProtKB: Tape measure protein

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Macromolecule #23: gp53, baseplate wedge protein

MacromoleculeName: gp53, baseplate wedge protein / type: protein_or_peptide / ID: 23 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 22.990885 KDa
SequenceString: MLFTFFDPIE YAAKTVNKNA PTIPMTDIFR NYKDYFKRAL AGYRLRTYYI KGSPRPEELA NAIYGNPQLY WVLLMCNDNY DPYYGWITS QEAAYQASIQ KYKNVGGDQI VYHVNENGEK FYNLISYDDN PYVWYDKGDK ARKYPQYEGA LAAVDTYEAA V LENEKLRQ ...String:
MLFTFFDPIE YAAKTVNKNA PTIPMTDIFR NYKDYFKRAL AGYRLRTYYI KGSPRPEELA NAIYGNPQLY WVLLMCNDNY DPYYGWITS QEAAYQASIQ KYKNVGGDQI VYHVNENGEK FYNLISYDDN PYVWYDKGDK ARKYPQYEGA LAAVDTYEAA V LENEKLRQ IKIIAKSDIN SFMNDLIRIM EKSYGNDK

UniProtKB: Baseplate wedge subunit

+
Macromolecule #24: gp25, baseplate protein

MacromoleculeName: gp25, baseplate protein / type: protein_or_peptide / ID: 24 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 15.111101 KDa
SequenceString:
MANINKLYSD IDPEMKMDWN KDVSRSLGLR SIKNSLLGII TTRKGSRPFD PEFGCDLSDQ LFENMTPLTA DTVERNIESA VRNYEPRID KLAVNVIPVY DDYTLIVEIR FSVIDNPDDI EQIKLQLASS NRV

UniProtKB: Baseplate wedge subunit

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Macromolecule #25: gp48, baseplate protein

MacromoleculeName: gp48, baseplate protein / type: protein_or_peptide / ID: 25 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 39.770332 KDa
SequenceString: MAIVKEITAD LIKKSGEKIS AGQSTKSEVG TKTYTAQFPT GRASGNDTTE DFQVTDLYKN GLLFTAYNMS SRDSGSLRSM RSNYSSSSS SILRTARNTI SSTVSKLSNG LISNNNSGTI SKSPIANILL PRSKSDVDTS SHRFNDVQES LISRGGGTAT G VLSNIAST ...String:
MAIVKEITAD LIKKSGEKIS AGQSTKSEVG TKTYTAQFPT GRASGNDTTE DFQVTDLYKN GLLFTAYNMS SRDSGSLRSM RSNYSSSSS SILRTARNTI SSTVSKLSNG LISNNNSGTI SKSPIANILL PRSKSDVDTS SHRFNDVQES LISRGGGTAT G VLSNIAST AVFGALESIT QGIMADNNEQ IYTTARSMYG GAENRTKVFT WDLTPRSTED LMAIINIYQY FNYFSYGETG KS QYAAEIK GYLDDWYRST LIEPLSPEDA AKNKTLFEKM TSSLTNVLVV SNPTVWMVKN FGATSKFDGK TEIFGPCQIQ SIR FDKTPN GNFNGLAIAP NLPSTFTLEI TMREIITLNR ASLYAGTF

UniProtKB: Baseplate tail tube cap

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Macromolecule #26: gp54, baseplate protein

MacromoleculeName: gp54, baseplate protein / type: protein_or_peptide / ID: 26 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 35.001188 KDa
SequenceString: MYSLEEFNNQ AINADFQRNN MFSCVFATTP STKSSSLISS ISNFSYNNLG LNSDWLGLTQ GDINQGITTL ITAGTQKLIR KSGVSKYLI GAMSQRTVQS LLGSFTVGTY LIDFFNMAYN SSGLMIYSVK MPENRLSYET DWNYNSPNIR ITGRELDPLV I SFRMDSEA ...String:
MYSLEEFNNQ AINADFQRNN MFSCVFATTP STKSSSLISS ISNFSYNNLG LNSDWLGLTQ GDINQGITTL ITAGTQKLIR KSGVSKYLI GAMSQRTVQS LLGSFTVGTY LIDFFNMAYN SSGLMIYSVK MPENRLSYET DWNYNSPNIR ITGRELDPLV I SFRMDSEA CNYRAMQDWV NSVQDPVTGL RALPQDVEAD IQVNLHSRNG LPHTAVMFTM HSISVSAPEL SYDGDNQITT FD VTFAYRV MQAGAVDRQR ALEWLESAAI NGIQSVLGNS GGVTGLSNSL SRLSRLGGTA GSISNINTMT GIVNSQSKIL GAI

UniProtKB: Baseplate tail-tube junction protein gp54

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Macromolecule #27: unassigned peptide A

MacromoleculeName: unassigned peptide A / type: protein_or_peptide / ID: 27 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 2.145636 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #28: unassigned peptide B

MacromoleculeName: unassigned peptide B / type: protein_or_peptide / ID: 28 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 1.549902 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

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Macromolecule #29: unassigned peptide C

MacromoleculeName: unassigned peptide C / type: protein_or_peptide / ID: 29 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 869.063 Da
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #30: unassigned peptide D

MacromoleculeName: unassigned peptide D / type: protein_or_peptide / ID: 30 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 613.749 Da
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #31: unassigned peptide E

MacromoleculeName: unassigned peptide E / type: protein_or_peptide / ID: 31 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 783.958 Da
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #32: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 32 / Number of copies: 6 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridModel: PELCO Ultrathin Carbon with Lacey Carbon / Support film - Material: CARBON / Support film - topology: LACEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
Details: Ted Pella lacey carbon 400 mesh grids (catalog No: 01824) were used..

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 38.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF
Details: This is the composite map based on the asymmetric reconstruction focused on the phage neck, the 3-fold-symmetric reconstruction focused on the middle part of the tail, and the 3-fold- ...Details: This is the composite map based on the asymmetric reconstruction focused on the phage neck, the 3-fold-symmetric reconstruction focused on the middle part of the tail, and the 3-fold-symmetric reconstruction focused on the baseplate. The resolutions of these reconstructions are 3.8A, 3.4A and 3.6A, respectively. (The number of particles is given for the asymmetric reconstruction focused on the phage neck.)
Number images used: 69000
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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Atomic model buiding 1

DetailsThe structures were fitted into the composite map using ChimeraX.
Output model

PDB-9mkb:
Structure of the bacteriophage T4 portal-neck-tail complex

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Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

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