EMDB-48459: Structure of the distal part of the bacteriophage T4 tail

Basic information

Entry

Database: EMDB / ID: EMD-48459

• Title: Structure of the distal part of the bacteriophage T4 tail
• Map data: 3-fold-symmetric reconstruction focused on the bacteriophage T4 baseplate

Sample

• Virus: Escherichia phage T4 (virus)
  • Protein or peptide: x 9 types
• Protein or peptide: x 13 types
• Ligand: x 1 types

• Keywords: bacteriophage T4 / contractile tail / baseplate / VIRAL PROTEIN

Function / homology

Function:

virus tail, sheath / symbiont genome ejection through host cell envelope, contractile tail mechanism / symbiont entry into host cell via disruption of host cell wall peptidoglycan / virus tail, baseplate / viral tail assembly / virus tail, fiber / symbiont entry into host cell via disruption of host cell envelope / viral release from host cell / peptidoglycan catabolic process / virion component / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / entry receptor-mediated virion attachment to host cell / defense response to bacterium / symbiont entry into host cell / structural molecule activity / metal ion binding / identical protein binding

Domain/homology:

Bacteriophage T4, Gp12 / Baseplate tail-tube protein gp48, T4-like virus / Short tail fibre protein, C-terminal / Short tail fibre protein, C-terminal superfamily / Phage short tail fibre protein gp12, middle domain / Tail-tube assembly protein / Short tail fibre protein receptor-binding domain / Baseplate wedge protein gp53, bacteriophage T4 / Baseplate wedge protein gp7 / : / : / : / Base plate wedge protein 53 / Baseplate wedge protein gp7, domain V / Baseplate wedge protein gp7, helical domain / Baseplate wedge protein gp7, domain VI / Bacteriophage T4, Gp27, baseplate hub, C-terminal / Bacteriophage T4, Gp27, baseplate hub, N-terminal / Gp27, baseplate hub, domain 3 / Gp27, baseplate hub, domain 4 / Gp27, baseplate hub, domain 2 / Baseplate structural protein, domain 2 / Baseplate structural protein, domain 1 / Baseplate structural protein Gp11 / Bacteriophage T4, Gp11, C-terminal finger domain / Baseplate structural protein Gp11, N-terminal domain superfamily / Baseplate structural protein Gp11 superfamily / Baseplate structural protein Gp11, C-terminal domain / GP11 baseplate wedge protein / Baseplate wedge protein gp10 / Baseplate wedge protein gp6 / Phage tail collar domain superfamily / : / : / : / : / : / : / Baseplate wedge protein gp6-like, helical domain / Baseplate structural protein gp6, C-terminal domain I / Baseplate structural protein gp6, C-terminal domain II / Baseplate wedge protein gp6, domain II / Baseplate structural protein gp6, C-terminal domain III / Baseplate wedge protein gp10 domain 3 / : / Tail sheath protein Gp18-like domain I / Phage tail collar domain / Baseplate structural protein Gp9 C-terminal domain superfamily / Phage Tail Collar Domain / Bacteriophage T4, Gp8 / Bacteriophage T4, Gp8 superfamily / Bacteriophage T4, Gp8 / Gp5, C-terminal / Gp5 C-terminal repeat (3 copies) / : / Baseplate structural protein Gp10, C-terminal domain / Baseplate structural protein Gp9/Gp10 / Baseplate structural protein Gp9/Gp10 middle domain superfamily / Gp9-like superfamily / : / Bacteriophage T4 gp9/10-like protein, N-terminal / Bacteriophage T4 gp9/10-like protein, C-terminal / Protein Gp5, N-terminal OB-fold domain / Pre-baseplate central spike protein Gp5 / Gp5 N-terminal OB domain / : / Tail sheath protein Gp18 domain III N-terminal region / IraD/Gp25-like / PAAR motif / Baseplate wedge protein gp25 / PAAR motif / Phage tail sheath protein, beta-sandwich domain / Phage tail sheath protein beta-sandwich domain / Bacteriophage T4, Gp19, tail tube / T4-like virus tail tube protein gp19 / : / Tail sheath protein, subtilisin-like domain / Phage tail sheath protein subtilisin-like domain / Tail sheath protein, C-terminal domain / Phage tail sheath C-terminal domain / T4-type lysozyme / : / Glycoside hydrolase, family 24 / Phage lysozyme / Lysozyme domain superfamily / Fibronectin type III / Fibronectin type III superfamily / Lysozyme-like domain superfamily / Immunoglobulin-like fold / Peptidase S1, PA clan, chymotrypsin-like fold

Component:

Pre-baseplate central spike protein Gp5 / Tail tube protein / Phospholipase / Baseplate tail tube cap / Tail sheath protein / Baseplate wedge subunit / Baseplate wedge subunit and tail pin / Baseplate wedge subunit / Baseplate wedge tail fiber connector / Baseplate hub subunit / Baseplate wedge subunit / Baseplate wedge protein gp10 / Short tail fiber protein gp12 / Tape measure protein / Baseplate tail-tube junction protein gp54 / Baseplate wedge protein gp6 / Baseplate wedge protein gp7

• Biological species: Escherichia phage T4 (virus)
• Method: single particle reconstruction / cryo EM / Resolution: 3.6 Å
• Authors: Fokine A / Zhu J / Klose T / Vago F / Arnaud C / Wang Z / Khare B / Rossmann MG / Chen Z / Sun L / Fang Q / Kuhn R / Rao VB

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	AI081726	United States

• Citation: Journal: To Be Published; Title: In situ Atomic Structure of Phage T4 Head-Tail Connector Complex Shows Molecular Gates Open for Genome Ejection and DNA Pushed into the Tail Tube; Authors: Fokine A / Zhu J / Klose T / Vago F / Arnaud C / Wang Z / Khare B / Rossmann MG / Chen Z / Sun L / Fang Q / Kuhn R / Rao VB

History

Deposition	Dec 26, 2024	-
Header (metadata) release	Jan 28, 2026	-
Map release	Jan 28, 2026	-
Update	Jan 28, 2026	-
Current status	Jan 28, 2026	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_48459.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: 3-fold-symmetric reconstruction focused on the bacteriophage T4 baseplate
• Voxel size: X=Y=Z: 1.616 Å

Density

Contour Level	By AUTHOR: 0.015
Minimum - Maximum	-0.07217898 - 0.10107589
Average (Standard dev.)	0.000031823056 (±0.005151396)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 646.4 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_48459_msk_1.map

Half map: #1

• File: emd_48459_half_map_1.map

Half map: #2

• File: emd_48459_half_map_2.map

Sample components

Entire : Escherichia phage T4

• Entire: Name: Escherichia phage T4 (virus)

Components

• Virus: Escherichia phage T4 (virus)
  • Protein or peptide: gp12, short tail fiber protein
  • Protein or peptide: gp9, baseplate wedge tail fiber connector
  • Protein or peptide: gp11, baseplate wedge protein
  • Protein or peptide: gp10, baseplate wedge protein
  • Protein or peptide: gp7, baseplate wedge protein
  • Protein or peptide: gp8, baseplate wedge protein
  • Protein or peptide: gp18, tail sheath protein
  • Protein or peptide: gp6, baseplate wedge protein
  • Protein or peptide: gp5, baseplate central spike protein
• Protein or peptide: gp5.4, tip of the central spike
• Protein or peptide: gp19, tail tube protein
• Protein or peptide: gp27, baseplate hub protein
• Protein or peptide: gp29, tape measure protein
• Protein or peptide: gp53, baseplate wedge protein
• Protein or peptide: gp25, baseplate wedge protein
• Protein or peptide: gp48, baseplate protein
• Protein or peptide: gp54, baseplate protein
• Protein or peptide: unassigned peptide A
• Protein or peptide: unassigned peptide B
• Protein or peptide: unassigned peptide C
• Protein or peptide: unassigned peptide D
• Protein or peptide: unassigned peptide E
• Ligand: ZINC ION

Supramolecule #1: Escherichia phage T4

• Supramolecule: Name: Escherichia phage T4 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1-#9 / NCBI-ID: 2681598 / Sci species name: Escherichia phage T4 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No
• Host (natural): Organism: Escherichia coli (E. coli) / Strain: B

Macromolecule #1: gp12, short tail fiber protein

• Macromolecule: Name: gp12, short tail fiber protein / type: protein_or_peptide / ID: 1 / Number of copies: 18 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 56.253926 KDa

Sequence

String:

MSNNTYQHVS NESRYVKFDP TDTNFPPEIT DVQAAIAAIS PAGVNGVPDA SSTTKGILFI PTEQEVIDGT NNTKAVTPAT LATRLSYPN ATETVYGLTR YSTNDEAIAG VNNESSITPA KFTVALNNAF ETRVSTESSN GVIKISSLPQ ALAGADDTTA M TPLKTQQL AIKLIAQIAP SETTATESDQ GVVQLATVAQ VRQGTLREGY AISPYTFMNS SSTEEYKGVI KLGTQSEVNS NN ASVAVTG ATLNGRGSTT SMRGVVKLTT TAGSQSGGDA SSALAWNADV IQQRGGQIIY GTLRIEDTFT IANGGANITG TVR MTGGYI QGNRIVTQNE IDRTIPVGAI MMWAADSLPS DAWRFCHGGT VSASDCPLYA SRIGTRYGGN PSNPGLPDMR GLFV RGSGR GSHLTNPNVN GNDQFGKPRL GVGCTGGYVG EVQIQQMSYH KHAGGFGEHD DLGAFGNTRR SNFVGTRKGL DWDNR SYFT NDGYEIDPES QRNSKYTLNR PELIGNETRP WNISLNYIIK VKE

UniProtKB: Short tail fiber protein gp12

Macromolecule #2: gp9, baseplate wedge tail fiber connector

• Macromolecule: Name: gp9, baseplate wedge tail fiber connector / type: protein_or_peptide / ID: 2 / Number of copies: 18 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 31.024725 KDa

Sequence

String:

MFIQEPKKLI DTGEIGNAST GDILFDGGNK INSDFNAIYN AFGDQRKMAV ANGTGADGQI IHATGYYQKH SITEYATPVK VGTRHDIDT STVGVKVIIE RGELGDCVEF INSNGSISVT NPLTIQAIDS IKGVSGNLVV TSPYSKVTLR CISSDNSTSV W NYSIESMF GQKESPAEGT WNISTSGSVD IPLFHRTEYN MAKLLVTCQS VDGRKIKTAE INILVDTVNS EVISSEYAVM RV GNETEED EIANIAFSIK ENYVTATISS STVGMRAAVK VIATQKIGVA Q

UniProtKB: Baseplate wedge tail fiber connector

Macromolecule #3: gp11, baseplate wedge protein

• Macromolecule: Name: gp11, baseplate wedge protein / type: protein_or_peptide / ID: 3 / Number of copies: 18 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 23.725523 KDa

Sequence

String:

MSLLNNKAGV ISRLADFLGF RPKTGDIDVM NRQSVGSVTI SQLAKGFYEP NIESAINDVH NFSIKDVGTI ITNKTGVSPE GVSQTDYWA FSGTVTDDSL PPGSPITVLV FGLPVSATTG MTAIEFVAKV RVALQEAIAS FTAINSYKDH PTDGSKLEVT Y LDNQKHVL STYSTYGITI SQEIISESKP GYGTWNLLGA QTVTLDNQQT PTVFYHFERT A

UniProtKB: Baseplate wedge subunit and tail pin

Macromolecule #4: gp10, baseplate wedge protein

• Macromolecule: Name: gp10, baseplate wedge protein / type: protein_or_peptide / ID: 4 / Number of copies: 18 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 66.28168 KDa

Sequence

String:

MKQNINIGNV VDDGTGDYLR KGGIKINENF DELYYELGDG DVPYSAGAWK TYNASSGQTL TAEWGKSYAI NTSSGRVTIN LPKGTVNDY NKVIRARDVF ATWNVNPVTL VAASGDTIKG SAVPVEINVR FSDLELVYCA PGRWEYVKNK QIDKITSSDI S NVARKEFL VEVQGQTDFL DVFRGTSYNV NNIRVKHRGN ELYYGDVFSE NSDFGSPGEN EGELVPLDGF NIRLRQPCNI GD TVQIETF MDGVSQWRSS YTRRQIRLLD SKLTSKTSLE GSIYVTDLST MKSIPFSAFG LIPGEPINPN SLEVRFNGIL QEL AGTVGM PLFHCVGADS DDEVECSVLG GTWEQSHTDY SVETDENGIP EILHFDSVFE HGDIINITWF NNDLGTLLTK DEII DETDN LYVSQGPGVD ISGDVNLTDF DKIGWPNVEA VQSYQRAFNA VSNIFDTIYP IGTIYENAVN PNNPVTYMGF GSWKL FGQG KVLVGWNEDI SDPNFALNNN DLDSGGNPSH TAGGTGGSTS VTLENANLPA TETDEEVLIV DENGSVIVGG CQYDPD ESG PIYTKYREAK ASTNSTHTPP TSITNIQPYI TVYRWIRIA

UniProtKB: Baseplate wedge protein gp10

Macromolecule #5: gp7, baseplate wedge protein

• Macromolecule: Name: gp7, baseplate wedge protein / type: protein_or_peptide / ID: 5 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 119.336516 KDa

Sequence

String:

MTVKAPSVTS LRISKLSANQ VQVRWDDVGA NFYYFVEIAE TKTNSGENLP SNQYRWINLG YTANNSFFFD DADPLTTYII RVATAAQDF EQSDWIYTEE FETFATNAYT FQNMIEMQLA NKFIQEKFTL NNSDYVNFNN DTIMAALMNE SFQFSPSYVD V SSISNFII GENEYHEIQG SIQQVCKDIN RVYLMESEGI LYLFERYQPV VKVSNDKGQT WKAVKLFNDR VGYPLSKTVY YQ SANTTYV LGYDKIFYGR KSTDVRWSAD DVRFSSQDIT FAKLGDQLHL GFDVEIFATY ATLPANVYRI AEAITCTDDY IYV VARDKV RYIKTSNALI DFDPLSPTYS ERLFEPDTMT ITGNPKAVCY KMDSICDKVF ALIIGEVETL NANPRTSKII DSAD KGIYV LNHDEKTWKR VFGNTEEERR RIQPGYANMS TDGKLVSLSS SNFKFLSDNV VNDPETAAKY QLIGAVKYEF PREWL ADKH YHMMAFIADE TSDWETFTPQ PMKYYAEPFF NWSKKSNTRC WINNSDRAVV VYADLKYTKV IENIPETSPD RLVHEY WDD GDCTIVMPNV KFTGFKKYAS GMLFYKASGE IISYYDFNYR VRDTVEIIWK PTEVFLKAFL QNQEHETPWS PEEERGL AD PDLRPLIGTM MPDSYLLQDS NFEAFCEAYI QYLSDGYGTQ YNNLRNLIRN QYPREEHAWE YLWSEIYKRN IYLNADKR D AVARFFESRS YDFYSTKGIE ASYKFLFKVL YNEEVEIEIE SGAGTEYDII VQSDSLTEDL VGQTIYTATG RCNVTYIER SYSNGKLQWT VTIHNLLGRL IAGQEVKAER LPSFEGEIIR GVKGKDLLQN NIDYINRSRS YYVMKIKSNL PSSRWKSDVI RFVHPVGFG FIAITLLTMF INVGLTLKHT ETIINKYKNY KWDSGLPTEY ADRIAKLTPT GEIEHDSVTG EAIYEPGPMA G VKYPLPDD YNAENNNSIF QGQLPSERRK LMSPLFDASG TTFAQFRDLV NKRLKDNIGN PRDPENPTQV KIDE

UniProtKB: Baseplate wedge protein gp7

Macromolecule #6: gp8, baseplate wedge protein

• Macromolecule: Name: gp8, baseplate wedge protein / type: protein_or_peptide / ID: 6 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 38.041668 KDa

Sequence

String:

MNDSSVIYRA IVTSKFRTEK MLNFYNSIGS GPDKNTIFIT FGRSEPWSSN ENEVGFAPPY PTDSVLGVTD MWTHMMGTVK VLPSMLDAV IPRRDWGDTR YPDPYTFRIN DIVVCNSAPY NATESGAGWL VYRCLDVPDT GMCSIASLTD KDECLKLGGK W TPSARSMT PPEGRGDAEG TIEPGDGYVW EYLFEIPPDV SINRCTNEYI VVPWPEELKE DPTRWGYEDN LTWQQDDFGL IY RVKANTI RFKAYLDSVY FPEAALPGNK GFRQISIITN PLEAKAHPND PNVKAEKDYY DPEDLMRHSG EMIYMENRPP IIM AMDQTE EINILFTF

UniProtKB: Baseplate wedge subunit

Macromolecule #7: gp18, tail sheath protein

• Macromolecule: Name: gp18, tail sheath protein / type: protein_or_peptide / ID: 7 / Number of copies: 36 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 71.348625 KDa

Sequence

String:

MTLLSPGIEL KETTVQSTVV NNSTGTAALA GKFQWGPAFQ IKQVTNEVDL VNTFGQPTAE TADYFMSAMN FLQYGNDLRV VRAVDRDTA KNSSPIAGNI EYTISTPGSN YAVGDKITVK YVSDDIETEG KITEVDADGK IKKINIPTAK IIAKAKEVGE Y PTLGSNWT AEISSSSSGL AAVITLGKII TDSGILLAEI ENAEAAMTAV DFQANLKKYG IPGVVALYPG ELGDKIEIEI VS KADYAKG ASALLPIYPG GGTRASTAKA VFGYGPQTDS QYAIIVRRND AIVQSVVLST KRGGKDIYDS NIYIDDFFAK GGS EYIFAT AQNWPEGFSG ILTLSGGLSS NAEVTAGDLM EAWDFFADRE SVDVQLFIAG SCAGESLETA STVQKHVVSI GDVR QDCLV LCSPPRETVV GIPVTRAVDN LVNWRTAAGS YTDNNFNISS TYAAIDGNYK YQYDKYNDVN RWVPLAADIA GLCAR TDNV SQTWMSPAGY NRGQILNVIK LAIETRQAQR DRLYQEAINP VTGTGGDGYV LYGDKTATSV PSPFDRINVR RLFNML KTN IGRSSKYRLF ELNNAFTRSS FRTETAQYLQ GIKALGGIYE YRVVCDTTNN TPSVIDRNEF VATFYIQPAR SINYITL NF VATATGADFD ELTGLAG

UniProtKB: Tail sheath protein

Macromolecule #8: gp6, baseplate wedge protein

• Macromolecule: Name: gp6, baseplate wedge protein / type: protein_or_peptide / ID: 8 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 74.492641 KDa

Sequence

String:

MANTPVNYQL TRTANAIPEI FVGGTFAEIK QNLIEWLNGQ NEFLDYDFEG SRLNVLCDLL AYNTLYIQQF GNAAVYESFM RTANLRSSV VQAAQDNGYL PTSKSAAQTE IMLTCTDALN RNYITIPRGT RFLAYAKDTS VNPYNFVSRE DVIAIRDKNN Q YFPRLKLA QGRIVRTEII YDKLTPIIIY DKNIDRNQVK LYVDGAEWIN WTRKSMVHAG STSTIYYMRE TIDGNTEFYF GE GEISVNA SEGALTANYI GGLKPTQNST IVIEYISTNG ADANGAVGFS YADTLTNITV ININENPNDD PDFVGADGGG DPE DIERIR ELGTIKRETQ QRCVTATDYD TFVSERFGSI IQAVQTFTDS TKPGYAFIAA KPKSGLYLTT VQREDIKNYL KDYN LAPIT PSIISPNYLF IKTNLKVTYA LNKLQESEQW LEGQIIDKID RYYTEDVEIF NSSFAKSKML TYVDDADHSV IGSSA TIQM VREVQNFYKT PEAGIKYNNQ IKDRSMESNT FSFNSGRKVV NPDTGLEEDV LYDVRIVSTD RDSKGIGKVI IGPFAS GDV TENENIQPYT GNDFNKLANS DGRDKYYVIG EINYPADVIY WNIAKINLTS EKFEVQTIEL YSDPTDDVIF TRDGSLI VF ENDLRPQYLT IDLEPISQ

UniProtKB: Baseplate wedge protein gp6

Macromolecule #9: gp5, baseplate central spike protein

• Macromolecule: Name: gp5, baseplate central spike protein / type: protein_or_peptide / ID: 9 / Number of copies: 3 / Enantiomer: LEVO / EC number: lysozyme
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 63.183723 KDa

Sequence

String:

MEMISNNLNW FVGVVEDRMD PLKLGRVRVR VVGLHPPQRA QGDVMGIPTE KLPWMSVIQP ITSAAMSGIG GSVTGPVEGT RVYGHFLDK WKTNGIVLGT YGGIVREKPN RLEGFSDPTG QYPRRLGNDT NVLNQGGEVG YDSSSNVIQD SNLDTAINPD D RPLSEIPT DDNPNMSMAE MLRRDEGLRL KVYWDTEGYP TIGIGHLIMK QPVRDMAQIN KVLSKQVGRE ITGNPGSITM EE ATTLFER DLADMQRDIK SHSKVGPVWQ AVNRSRQMAL ENMAFQMGVG GVAKFNTMLT AMLAGDWEKA YKAGRDSLWY QQT KGRASR VTMIILTGNL ESYGVEVKTP ARSLSAMAAT VAKSSDPADP PIPNDSRILF KEPVSSYKGE YPYVHTMETE SGHI QEFDD TPGQERYRLV HPTGTYEEVS PSGRRTRKTV DNLYDITNAD GNFLVAGDKK TNVGGSEIYY NMDNRLHQID GSNTI FVRG DETKTVEGNG TILVKGNVTI IVEGNADITV KGDATTLVEG NQTNTVNGNL SWKVAGTVDW DVGGDWTEKM ASMSSI SSG QYTIDGSRID IG

UniProtKB: Pre-baseplate central spike protein Gp5

Macromolecule #10: gp5.4, tip of the central spike

• Macromolecule: Name: gp5.4, tip of the central spike / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 10.233663 KDa

Sequence

String:

MSGLSYDKCV TAGHEAWPPT VVNATQSKVF TGGIAVLVAG DPITEHTEIK KPYETHGGVT QPRTSKVYVT GKKAVQMADP ISCGDTVAQ ASSKVFIK

UniProtKB: Phospholipase

Macromolecule #11: gp19, tail tube protein

• Macromolecule: Name: gp19, tail tube protein / type: protein_or_peptide / ID: 11 / Number of copies: 30 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 18.479613 KDa

Sequence

String:

MFVDDVTRAF ESGDFARPNL FQVEISYLGQ NFTFQCKATA LPAGIVEKIP VGFMNRKINV AGDRTFDDWT VTVMNDEAHD ARQKFVDWQ SIAAGQGNEI TGGKPAEYKK SAIVRQYARD AKTVTKEIEI KGLWPTNVGE LQLDWDSNNE IQTFEVTLAL D YWE

UniProtKB: Tail tube protein

Macromolecule #12: gp27, baseplate hub protein

• Macromolecule: Name: gp27, baseplate hub protein / type: protein_or_peptide / ID: 12 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 44.431062 KDa

Sequence

String:

MSMLQRPGYP NLSVKLFDSY DAWSNNRFVE LAATITTLTM RDSLYGRNEG MLQFYDSKNI HTKMDGNEII QISVANANDI NNVKTRIYG CKHFSVSVDS KGDNIIAIEL GTIHSIENLK FGRPFFPDAG ESIKEMLGVI YQDRTLLTPA INAINAYVPD I PWTSTFEN YLSYVREVAL AVGSDKFVFV WQDIMGVNMM DYDMMINQEP YPMIVGEPSL IGQFIQELKY PLAYDFVWLT KS NPHKRDP MKNATIYAHS FLDSSIPMIT TGKGENSIVV SRSGAYSEMT YRNGYEEAIR LQTMAQYDGY AKCSTIGNFN LTP GVKIIF NDSKNQFKTE FYVDEVIHEL SNNNSVTHLY MFTNATKLET IDPVKVKNEF KSDTTTEESS SSNKQ

UniProtKB: Baseplate hub subunit

Macromolecule #13: gp29, tape measure protein

• Macromolecule: Name: gp29, tape measure protein / type: protein_or_peptide / ID: 13 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 64.485539 KDa

Sequence

String:

MKKPQEMQTM RRKVISDNKP TQEAAKSASN TLSGLNDIST KLDDAQAASE LIAQTVEEKS NEIIGAIDNV ESAVSDTSAG SELIAETVE IGNNINKEIG ESLGSKLDKL TSLLEQKIQT AGIQQTGTSL ATVESAIPVK VVEDDTAESV GPLLPAPEAV N NDPDADFF PTPQPVEPKQ ESPEEKQKKE AFNLKLSQAL DKLTKTVDFG FKKSISITDK ISSMLFKYTV SAAIEAAKMT AM ILAVVVG IDLLMIHFKY WSDKFSKAWD LFSTDFTKFS SETGTWGPLL QSIFDSIDKI KQLWEAGDWG GLTVAIVEGL GKV LFNLGE LIQLGMAKLS AAILRVIPGM KDTADEVEGR ALENFQNSTG ASLNKEDQEK VANYQDKRMN GDLGPIAEGL DKIS NWKTR ASNWIRGVDN KEALTTDEER AAEEEKLKQL SPEERKNALM KANEARAAMI RFEKYADSAD MSKDSTVKSV EAAYE DLKK RMDDPDLNNS PAVKKELAAR FSKIDATYQE LKKNQPNAKP ETSAKSPEAK QVQVIEKNKA QQAPVQQASP SINNTN NVI KKNTVVHNMT PVTSTTAPGV FDATGVN

UniProtKB: Tape measure protein

Macromolecule #14: gp53, baseplate wedge protein

• Macromolecule: Name: gp53, baseplate wedge protein / type: protein_or_peptide / ID: 14 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 22.990885 KDa

Sequence

String:

MLFTFFDPIE YAAKTVNKNA PTIPMTDIFR NYKDYFKRAL AGYRLRTYYI KGSPRPEELA NAIYGNPQLY WVLLMCNDNY DPYYGWITS QEAAYQASIQ KYKNVGGDQI VYHVNENGEK FYNLISYDDN PYVWYDKGDK ARKYPQYEGA LAAVDTYEAA V LENEKLRQ IKIIAKSDIN SFMNDLIRIM EKSYGNDK

UniProtKB: Baseplate wedge subunit

Macromolecule #15: gp25, baseplate wedge protein

• Macromolecule: Name: gp25, baseplate wedge protein / type: protein_or_peptide / ID: 15 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 15.111101 KDa

Sequence

String:

MANINKLYSD IDPEMKMDWN KDVSRSLGLR SIKNSLLGII TTRKGSRPFD PEFGCDLSDQ LFENMTPLTA DTVERNIESA VRNYEPRID KLAVNVIPVY DDYTLIVEIR FSVIDNPDDI EQIKLQLASS NRV

UniProtKB: Baseplate wedge subunit

Macromolecule #16: gp48, baseplate protein

• Macromolecule: Name: gp48, baseplate protein / type: protein_or_peptide / ID: 16 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 39.770332 KDa

Sequence

String:

MAIVKEITAD LIKKSGEKIS AGQSTKSEVG TKTYTAQFPT GRASGNDTTE DFQVTDLYKN GLLFTAYNMS SRDSGSLRSM RSNYSSSSS SILRTARNTI SSTVSKLSNG LISNNNSGTI SKSPIANILL PRSKSDVDTS SHRFNDVQES LISRGGGTAT G VLSNIAST AVFGALESIT QGIMADNNEQ IYTTARSMYG GAENRTKVFT WDLTPRSTED LMAIINIYQY FNYFSYGETG KS QYAAEIK GYLDDWYRST LIEPLSPEDA AKNKTLFEKM TSSLTNVLVV SNPTVWMVKN FGATSKFDGK TEIFGPCQIQ SIR FDKTPN GNFNGLAIAP NLPSTFTLEI TMREIITLNR ASLYAGTF

UniProtKB: Baseplate tail tube cap

Macromolecule #17: gp54, baseplate protein

• Macromolecule: Name: gp54, baseplate protein / type: protein_or_peptide / ID: 17 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 35.001188 KDa

Sequence

String:

MYSLEEFNNQ AINADFQRNN MFSCVFATTP STKSSSLISS ISNFSYNNLG LNSDWLGLTQ GDINQGITTL ITAGTQKLIR KSGVSKYLI GAMSQRTVQS LLGSFTVGTY LIDFFNMAYN SSGLMIYSVK MPENRLSYET DWNYNSPNIR ITGRELDPLV I SFRMDSEA CNYRAMQDWV NSVQDPVTGL RALPQDVEAD IQVNLHSRNG LPHTAVMFTM HSISVSAPEL SYDGDNQITT FD VTFAYRV MQAGAVDRQR ALEWLESAAI NGIQSVLGNS GGVTGLSNSL SRLSRLGGTA GSISNINTMT GIVNSQSKIL GAI

UniProtKB: Baseplate tail-tube junction protein gp54

Macromolecule #18: unassigned peptide A

• Macromolecule: Name: unassigned peptide A / type: protein_or_peptide / ID: 18 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 2.145636 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #19: unassigned peptide B

• Macromolecule: Name: unassigned peptide B / type: protein_or_peptide / ID: 19 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 1.549902 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #20: unassigned peptide C

• Macromolecule: Name: unassigned peptide C / type: protein_or_peptide / ID: 20 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 869.063 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #21: unassigned peptide D

• Macromolecule: Name: unassigned peptide D / type: protein_or_peptide / ID: 21 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 613.749 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #22: unassigned peptide E

• Macromolecule: Name: unassigned peptide E / type: protein_or_peptide / ID: 22 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia phage T4 (virus)
• Molecular weight: Theoretical: 783.958 Da

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #23: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 23 / Number of copies: 6 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.5
• Grid: Model: PELCO Ultrathin Carbon with Lacey Carbon / Support film - Material: CARBON / Support film - topology: LACEY
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV; Details: Ted Pella lacey carbon 400 mesh grids (catalog No: 01824) were used..

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 38.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: NONE
• Final reconstruction: Applied symmetry - Point group: C₃ (3 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 113800
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD