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- EMDB-48458: Structure of the bacteriophage T4 portal-neck-tail connector complex -

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Basic information

Entry
Database: EMDB / ID: EMD-48458
TitleStructure of the bacteriophage T4 portal-neck-tail connector complex
Map dataAsymmetric cryo-EM reconstruction focused on the neck region of T4 virion
Sample
  • Virus: Escherichia phage T4 (virus)
    • Protein or peptide: x 11 types
Keywordsbacteriophage T4 / portal vertex / phage neck / tail terminator protein / tube terminator protein / asymmetric phage reconstruction / DNA retention / neck stopper protein / head completion proteins / VIRAL PROTEIN
Function / homology
Function and homology information


virus tail, sheath / symbiont genome ejection through host cell envelope, contractile tail mechanism / viral capsid, decoration / T=13 icosahedral viral capsid / viral genome packaging / viral release from host cell / virion component / viral capsid / structural molecule activity
Similarity search - Function
Bacteriophage T4, Gp14, neck protein / Virus neck protein / Portal protein Gp20 / Bacteriophage T4-like portal protein (Gp20) / Highly immunogenic outer capsid protein / Small outer capsid protein / Myoviridae tail sheath stabiliser / Small outer capsid protein superfamily / Myoviridae tail sheath stabiliser superfamily / T4-like virus Myoviridae tail sheath stabiliser ...Bacteriophage T4, Gp14, neck protein / Virus neck protein / Portal protein Gp20 / Bacteriophage T4-like portal protein (Gp20) / Highly immunogenic outer capsid protein / Small outer capsid protein / Myoviridae tail sheath stabiliser / Small outer capsid protein superfamily / Myoviridae tail sheath stabiliser superfamily / T4-like virus Myoviridae tail sheath stabiliser / Small outer capsid protein / Major capsid protein, Myoviridae / Major capsid protein Gp23 / : / Tail sheath protein Gp18-like domain I / Capsid protein, T4-like bacteriophage-like / : / Tail sheath protein Gp18 domain III N-terminal region / Phage tail sheath protein, beta-sandwich domain / Phage tail sheath protein beta-sandwich domain / Bacteriophage T4, Gp19, tail tube / T4-like virus tail tube protein gp19 / PKD domain / : / Tail sheath protein, subtilisin-like domain / Phage tail sheath protein subtilisin-like domain / Tail sheath protein, C-terminal domain / Phage tail sheath C-terminal domain / PKD domain / PKD domain superfamily / PKD/Chitinase domain / Repeats in polycystic kidney disease 1 (PKD1) and other proteins / Fibritin C-terminal / Fibritin C-terminal region / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
Tail completion and sheath stabilizer protein / Tail tube protein / Small outer capsid protein / Tail sheath protein / Highly immunogenic outer capsid protein / Portal protein / Tail sheath stabilizer and completion protein / Major capsid protein / Fibritin / Neck protein gp13 / Neck protein gp14
Similarity search - Component
Biological speciesEscherichia phage T4 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsFokine A / Zhu J / Klose T / Vago F / Arnaud C / Wang Z / Khare B / Rossmann MG / Chen Z / Sun L ...Fokine A / Zhu J / Klose T / Vago F / Arnaud C / Wang Z / Khare B / Rossmann MG / Chen Z / Sun L / Fang Q / Kuhn R / Rao VB
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI081726 United States
CitationJournal: To Be Published
Title: In situ Atomic Structure of Phage T4 Head-Tail Connector Complex Shows Molecular Gates Open for Genome Ejection and DNA Pushed into the Tail Tube
Authors: Fokine A / Zhu J / Klose T / Vago F / Arnaud C / Wang Z / Khare B / Rossmann MG / Chen Z / Sun L / Fang Q / Kuhn R / Rao VB
History
DepositionDec 26, 2024-
Header (metadata) releaseJan 28, 2026-
Map releaseJan 28, 2026-
UpdateJan 28, 2026-
Current statusJan 28, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_48458.png

Downloads & links

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Map

FileDownload / File: emd_48458.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationAsymmetric cryo-EM reconstruction focused on the neck region of T4 virion
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.62 Å/pix.
x 400 pix.
= 646.4 Å		1.62 Å/pix.
x 400 pix.
= 646.4 Å		1.62 Å/pix.
x 400 pix.
= 646.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.616 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.015
Minimum - Maximum-0.05914059 - 0.08999042
Average (Standard dev.)-0.00001981072 (±0.0061150473)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 646.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_48458_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map B

Fileemd_48458_half_map_1.map
AnnotationHalf map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map A

Fileemd_48458_half_map_2.map
AnnotationHalf map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Escherichia phage T4

EntireName: Escherichia phage T4 (virus)
Components
  • Virus: Escherichia phage T4 (virus)
    • Protein or peptide: gp14, neck protein
    • Protein or peptide: gp18, tail sheath protein
    • Protein or peptide: gp19, tail tube protein
    • Protein or peptide: gpwac, fibritin
    • Protein or peptide: gp20, portal protein
    • Protein or peptide: gp23, major capsid protein
    • Protein or peptide: gp13, neck protein
    • Protein or peptide: gp15, tail terminator protein
    • Protein or peptide: gp3, tube terminator protein
    • Protein or peptide: Hoc, highly immunogenic outer capsid protein
    • Protein or peptide: Soc, small outer capsid protein

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Supramolecule #1: Escherichia phage T4

SupramoleculeName: Escherichia phage T4 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 2681598 / Sci species name: Escherichia phage T4 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Escherichia coli (E. coli) / Strain: B

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Macromolecule #1: gp14, neck protein

MacromoleculeName: gp14, neck protein / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 29.597836 KDa
SequenceString: MATYDKNLFA KLENRTGYSQ TNETEILNPY VNFNHYKNSQ ILADVLVAES IQMRGVECYY VPREYVSPDL IFGEDLKNKF TKAWKFAAY LNSFEGYEGA KSFFSNFGMQ VQDEVTLSIN PNLFKHQVNG KEPKEGDLIY FPMDNSLFEI NWVEPYDPFY Q LGQNAIRK ...String:
MATYDKNLFA KLENRTGYSQ TNETEILNPY VNFNHYKNSQ ILADVLVAES IQMRGVECYY VPREYVSPDL IFGEDLKNKF TKAWKFAAY LNSFEGYEGA KSFFSNFGMQ VQDEVTLSIN PNLFKHQVNG KEPKEGDLIY FPMDNSLFEI NWVEPYDPFY Q LGQNAIRK ITAGKFIYSG EEINPVLQKN EGINIPEFSE LELNAVRNLN GIHDINIDQY AEVDQINSEA KEYVEPYVVV NN RGKSFES SPFDNDFMD

UniProtKB: Neck protein gp14

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Macromolecule #2: gp18, tail sheath protein

MacromoleculeName: gp18, tail sheath protein / type: protein_or_peptide / ID: 2 / Number of copies: 30 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 71.348625 KDa
SequenceString: MTLLSPGIEL KETTVQSTVV NNSTGTAALA GKFQWGPAFQ IKQVTNEVDL VNTFGQPTAE TADYFMSAMN FLQYGNDLRV VRAVDRDTA KNSSPIAGNI EYTISTPGSN YAVGDKITVK YVSDDIETEG KITEVDADGK IKKINIPTAK IIAKAKEVGE Y PTLGSNWT ...String:
MTLLSPGIEL KETTVQSTVV NNSTGTAALA GKFQWGPAFQ IKQVTNEVDL VNTFGQPTAE TADYFMSAMN FLQYGNDLRV VRAVDRDTA KNSSPIAGNI EYTISTPGSN YAVGDKITVK YVSDDIETEG KITEVDADGK IKKINIPTAK IIAKAKEVGE Y PTLGSNWT AEISSSSSGL AAVITLGKII TDSGILLAEI ENAEAAMTAV DFQANLKKYG IPGVVALYPG ELGDKIEIEI VS KADYAKG ASALLPIYPG GGTRASTAKA VFGYGPQTDS QYAIIVRRND AIVQSVVLST KRGGKDIYDS NIYIDDFFAK GGS EYIFAT AQNWPEGFSG ILTLSGGLSS NAEVTAGDLM EAWDFFADRE SVDVQLFIAG SCAGESLETA STVQKHVVSI GDVR QDCLV LCSPPRETVV GIPVTRAVDN LVNWRTAAGS YTDNNFNISS TYAAIDGNYK YQYDKYNDVN RWVPLAADIA GLCAR TDNV SQTWMSPAGY NRGQILNVIK LAIETRQAQR DRLYQEAINP VTGTGGDGYV LYGDKTATSV PSPFDRINVR RLFNML KTN IGRSSKYRLF ELNNAFTRSS FRTETAQYLQ GIKALGGIYE YRVVCDTTNN TPSVIDRNEF VATFYIQPAR SINYITL NF VATATGADFD ELTGLAG

UniProtKB: Tail sheath protein

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Macromolecule #3: gp19, tail tube protein

MacromoleculeName: gp19, tail tube protein / type: protein_or_peptide / ID: 3 / Number of copies: 30 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 18.479613 KDa
SequenceString:
MFVDDVTRAF ESGDFARPNL FQVEISYLGQ NFTFQCKATA LPAGIVEKIP VGFMNRKINV AGDRTFDDWT VTVMNDEAHD ARQKFVDWQ SIAAGQGNEI TGGKPAEYKK SAIVRQYARD AKTVTKEIEI KGLWPTNVGE LQLDWDSNNE IQTFEVTLAL D YWE

UniProtKB: Tail tube protein

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Macromolecule #4: gpwac, fibritin

MacromoleculeName: gpwac, fibritin / type: protein_or_peptide / ID: 4 / Number of copies: 36 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 51.909164 KDa
SequenceString: MTDIVLNDLP FVDGPPAEGQ SRISWIKNGE EILGADTQYG SEGSMNRPTV SVLRNVEVLD KNIGILKTSL ETANSDIKTI QGILDVSGD IEALAQIGIN KKDISDLKTL TSEHTEILNG TNNTVDSILA DIGPFNAEAN SVYRTIRNDL LWIKRELGQY T GQDINGLP ...String:
MTDIVLNDLP FVDGPPAEGQ SRISWIKNGE EILGADTQYG SEGSMNRPTV SVLRNVEVLD KNIGILKTSL ETANSDIKTI QGILDVSGD IEALAQIGIN KKDISDLKTL TSEHTEILNG TNNTVDSILA DIGPFNAEAN SVYRTIRNDL LWIKRELGQY T GQDINGLP VVGNPSSGMK HRIINNTDVI TSQGIRLSEL ETKFIESDVG SLTIEVGNLR EELGPKPPSF SQNVYSRLNE ID TKQTTVE SDISAIKTSI GYPGNNSIIT SVNTNTDNIA SINLELNQSG GIKQRLTVIE TSIGSDDIPS SIKGQIKDNT TSI ESLNGI VGENTSSGLR ANVSWLNQIV GTDSSGGQPS PPGSLLNRVS TIETSVSGLN NAVQNLQVEI GNNSAGIKGQ VVAL NTLVN GTNPNGSTVE ERGLTNSIKA NETNIASVTQ EVNTAKGNIS SLQGDVQALQ EAGYIPEAPR DGQAYVRKDG EWVFL STFL SPA

UniProtKB: Fibritin

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Macromolecule #5: gp20, portal protein

MacromoleculeName: gp20, portal protein / type: protein_or_peptide / ID: 5 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 61.117082 KDa
SequenceString: MKFNVLSLFA PWAKMDERNF KDQEKEDLVS ITAPKLDDGA REFEVSSNEA ASPYNAAFQT IFGSYEPGMK TTRELIDTYR NLMNNYEVD NAVSEIVSDA IVYEDDTEVV ALNLDKSKFS PKIKNMMLDE FSDVLNHLSF QRKGSDHFRR WYVDSRIFFH K IIDPKRPK ...String:
MKFNVLSLFA PWAKMDERNF KDQEKEDLVS ITAPKLDDGA REFEVSSNEA ASPYNAAFQT IFGSYEPGMK TTRELIDTYR NLMNNYEVD NAVSEIVSDA IVYEDDTEVV ALNLDKSKFS PKIKNMMLDE FSDVLNHLSF QRKGSDHFRR WYVDSRIFFH K IIDPKRPK EGIKELRRLD PRQVQYVREI ITETEAGTKI VKGYKEYFIY DTAHESYACD GRMYEAGTKI KIPKAAVVYA HS GLVDCCG KNIIGYLHRA VKPANQLKLL EDAVVIYRIT RAPDRRVWYV DTGNMPARKA AEHMQHVMNT MKNRVVYDAS TGK IKNQQH NMSMTEDYWL QRRDGKAVTE VDTLPGADNT GNMEDIRWFR QALYMALRVP LSRIPQDQQG GVMFDSGTSI TRDE LTFAK FIRELQHKFE EVFLDPLKTN LLLKGIITED EWNDEINNIK IEFHRDSYFA ELKEAEILER RINMLTMAEP FIGKY ISHR TAMKDILQMT DEEIEQEAKQ IEEESKEARF QDPDQEQEDF

UniProtKB: Portal protein

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Macromolecule #6: gp23, major capsid protein

MacromoleculeName: gp23, major capsid protein / type: protein_or_peptide / ID: 6 / Number of copies: 30 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 56.074242 KDa
SequenceString: MTIKTKAELL NKWKPLLEGE GLPEIANSKQ AIIAKIFENQ EKDFQTAPEY KDEKIAQAFG SFLTEAEIGG DHGYNATNIA AGQTSGAVT QIGPAVMGMV RRAIPNLIAF DICGVQPMNS PTGQVFALRA VYGKDPVAAG AKEAFHPMYG PDAMFSGQGA A KKFPALAA ...String:
MTIKTKAELL NKWKPLLEGE GLPEIANSKQ AIIAKIFENQ EKDFQTAPEY KDEKIAQAFG SFLTEAEIGG DHGYNATNIA AGQTSGAVT QIGPAVMGMV RRAIPNLIAF DICGVQPMNS PTGQVFALRA VYGKDPVAAG AKEAFHPMYG PDAMFSGQGA A KKFPALAA STQTTVGDIY THFFQETGTV YLQASVQVTI DAGATDAAKL DAEIKKQMEA GALVEIAEGM ATSIAELQEG FN GSTDNPW NEMGFRIDKQ VIEAKSRQLK AAYSIELAQD LRAVHGMDAD AELSGILATE IMLEINREVV DWINYSAQVG KSG MTLTPG SKAGVFDFQD PIDIRGARWA GESFKALLFQ IDKEAVEIAR QTGRGEGNFI IASRNVVNVL ASVDTGISYA AQGL ATGFS TDTTKSVFAG VLGGKYRVYI DQYAKQDYFT VGYKGPNEMD AGIYYAPYVA LTPLRGSDPK NFQPVMGFKT RYGIG INPF AESAAQAPAS RIQSGMPSIL NSLGKNAYFR RVYVKGI

UniProtKB: Major capsid protein

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Macromolecule #7: gp13, neck protein

MacromoleculeName: gp13, neck protein / type: protein_or_peptide / ID: 7 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 34.772211 KDa
SequenceString: MSGYNPQNPK ELKDVILRRL GAPIINVELT PDQIYDCIQR ALELYGEYHF DGLNKGFHVF YVGDDEERYK TGVFDLRGSN VFAVTRILR TNIGSITSMD GNATYPWFTD FLLGMAGING GMGTSCNRFY GPNAFGADLG YFTQLTSYMG MMQDMLSPIP D FWFNSANE ...String:
MSGYNPQNPK ELKDVILRRL GAPIINVELT PDQIYDCIQR ALELYGEYHF DGLNKGFHVF YVGDDEERYK TGVFDLRGSN VFAVTRILR TNIGSITSMD GNATYPWFTD FLLGMAGING GMGTSCNRFY GPNAFGADLG YFTQLTSYMG MMQDMLSPIP D FWFNSANE QLKVMGNFQK YDLIIVESWT KSYIDTNKMV GNTVGYGTVG PQDSWSLSER YNNPDHNLVG RVVGQDPNVK QG AYNNRWV KDYATALAKE LNGQILARHQ GMMLPGGVTI DGQRLIEEAR LEKEALREEL YLLDPPFGIL VG

UniProtKB: Neck protein gp13

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Macromolecule #8: gp15, tail terminator protein

MacromoleculeName: gp15, tail terminator protein / type: protein_or_peptide / ID: 8 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 31.587486 KDa
SequenceString: MFGYFYNSSF RRYATLMGDL FSNIQIKRQL ESGDKFIRVP ITYASKEHFM MKLNKWTSIN SQEDVAKVET ILPRINLHLV DFSYNAPFK TNILNQNLLQ KGATSVVSQY NPSPIKMIYE LSIFTRYEDD MFQIVEQILP YFQPHFNTTM YEQFGNDIPF K RDIKIVLM ...String:
MFGYFYNSSF RRYATLMGDL FSNIQIKRQL ESGDKFIRVP ITYASKEHFM MKLNKWTSIN SQEDVAKVET ILPRINLHLV DFSYNAPFK TNILNQNLLQ KGATSVVSQY NPSPIKMIYE LSIFTRYEDD MFQIVEQILP YFQPHFNTTM YEQFGNDIPF K RDIKIVLM SAAIDEAIDG DNLSRRRIEW SLTFEVNGWM YPPVDDAEGL IRTTYTDFHA NTRDLPDGEG VFESVDSEVV PR DIDPEDW DGTVKQTFTS NVNRPTPPEP PGPRT

UniProtKB: Tail sheath stabilizer and completion protein

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Macromolecule #9: gp3, tube terminator protein

MacromoleculeName: gp3, tube terminator protein / type: protein_or_peptide / ID: 9 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 19.723143 KDa
SequenceString:
MSQALQQIFN QANTTNFVVS IPHSNTTSAF TLNAQSVPIP GIRIPVTDTV TGPFGLGRAQ RPGVTFEYDP LIVRFIVDEE LKSWIGMYE WMLGTSNYLT GENTAQKTGP EYITLYILDN SKTEIVMSIN FYKPWVSDLS EVEFSYTEDS DPALVCTATI P YTYFQVEK DGKIIAEV

UniProtKB: Tail completion and sheath stabilizer protein

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Macromolecule #10: Hoc, highly immunogenic outer capsid protein

MacromoleculeName: Hoc, highly immunogenic outer capsid protein / type: protein_or_peptide / ID: 10 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 40.416547 KDa
SequenceString: MTFTVDITPK TPTGVIDETK QFTATPSGQT GGGTITYAWS VDNVPQDGAE ATFSYVLKGP AGQKTIKVVA TNTLSEGGPE TAEATTTIT VKNKTQTTTL AVTPASPAAG VIGTPVQFTA ALASQPDGAS ATYQWYVDDS QVGGETNSTF SYTPTTSGVK R IKCVAQVT ...String:
MTFTVDITPK TPTGVIDETK QFTATPSGQT GGGTITYAWS VDNVPQDGAE ATFSYVLKGP AGQKTIKVVA TNTLSEGGPE TAEATTTIT VKNKTQTTTL AVTPASPAAG VIGTPVQFTA ALASQPDGAS ATYQWYVDDS QVGGETNSTF SYTPTTSGVK R IKCVAQVT ATDYDALSVT SNEVSLTVNK KTMNPQVTLT PPSINVQQDA SATFTANVTG APEEAQITYS WKKDSSPVEG ST NVYTVDT SSVGSQTIEV TATVTAADYN PVTVTKTGNV TVTAKVAPEP EGELPYVHPL PHRSSAYIWC GWWVMDEIQK MTE EGKDWK TDDPDSKYYL HRYTLQKMMK DYPEVDVQES RNGYIIHKTA LETGIIYTYP

UniProtKB: Highly immunogenic outer capsid protein

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Macromolecule #11: Soc, small outer capsid protein

MacromoleculeName: Soc, small outer capsid protein / type: protein_or_peptide / ID: 11 / Number of copies: 10 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage T4 (virus)
Molecular weightTheoretical: 9.129147 KDa
SequenceString:
MASTRGYVNI KTFEQKLDGN KKIEGKEISV AFPLYSDVHK ISGAHYQTFP SEKAAYSTVY EENQRTEWIA ANEDLWKVTG

UniProtKB: Small outer capsid protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridModel: PELCO Ultrathin Carbon with Lacey Carbon / Mesh: 400 / Support film - Material: CARBON / Support film - topology: LACEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec.
Details: The grids were glow-discharged using a PELCO easyGlow system for 60s at 25mA.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
Details: Ted Pella lacey carbon 400 mesh grids (catalog No: 01824) were used..

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 38.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 69000
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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