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- PDB-7y22: CryoEM structure of Klebsiella phage Kp7 tail complex applied wit... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7y22 | ||||||
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Title | CryoEM structure of Klebsiella phage Kp7 tail complex applied with C6 symmetry | ||||||
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Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huang, L. / Xiang, Y. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and assembly of the Klebsiella pneumoniae phage tail fibers Authors: Huang, L. / Xiang, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 445 KB | Display | ![]() |
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PDB format | ![]() | 364.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 33583MC ![]() 7xy1C ![]() 7xycC ![]() 7y1cC ![]() 7y23C ![]() 7y3tC ![]() 7y5sC M: map data used to model this data C: citing same article ( |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 58339.309 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Protein | Mass: 27228.775 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #3: Protein | | Mass: 87904.539 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() #4: Protein | Mass: 36062.914 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: ![]() |
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Sample preparation
Component | Name: Klebsiella phage Kp7 / Type: VIRUS / Entity ID: all / Source: NATURAL |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() |
Details of virus | Empty: NO / Enveloped: NO / Isolate: SPECIES / Type: VIRION |
Natural host | Organism: Klebsiella pneumoniae ATCC 43816 |
Virus shell | Diameter: 600 nm / Triangulation number (T number): 7 |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() |
Specimen support | Grid material: COPPER / Grid type: Quantifoil R2/2 |
Vitrification![]() | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Microscopy | Model: FEI TALOS ARCTICA |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() ![]() |
Image recording | Electron dose: 50 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
Image scans | Width: 3710 / Height: 3710 |
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Processing
EM software | Name: RELION / Version: 3.0.8 / Category: 3D reconstruction![]() |
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CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
Symmetry | Point symmetry![]() ![]() |
3D reconstruction![]() | Resolution: 4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 26075 / Symmetry type: POINT |
Atomic model building | B value: 47 / Protocol: AB INITIO MODEL / Space: REAL |