+Open data
-Basic information
Entry | Database: PDB / ID: 6yhs | |||||||||
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Title | Acinetobacter baumannii ribosome-amikacin complex - 50S subunit | |||||||||
Components |
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Keywords | RIBOSOME / antibiotic / amikacin / translation | |||||||||
Function / homology | Function and homology information large ribosomal subunit / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / tRNA binding / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome ...large ribosomal subunit / transferase activity / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / tRNA binding / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / cytoplasm Similarity search - Function | |||||||||
Biological species | Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 (bacteria) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.7 Å | |||||||||
Authors | Nicholson, D. / Edwards, T.A. / O'Neill, A.J. / Ranson, N.A. | |||||||||
Funding support | United Kingdom, 2items
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Citation | Journal: Structure / Year: 2020 Title: Structure of the 70S Ribosome from the Human Pathogen Acinetobacter baumannii in Complex with Clinically Relevant Antibiotics. Authors: David Nicholson / Thomas A Edwards / Alex J O'Neill / Neil A Ranson / Abstract: Acinetobacter baumannii is a Gram-negative bacterium primarily associated with hospital-acquired, often multidrug-resistant (MDR) infections. The ribosome-targeting antibiotics amikacin and ...Acinetobacter baumannii is a Gram-negative bacterium primarily associated with hospital-acquired, often multidrug-resistant (MDR) infections. The ribosome-targeting antibiotics amikacin and tigecycline are among the limited arsenal of drugs available for treatment of such infections. We present high-resolution structures of the 70S ribosome from A. baumannii in complex with these antibiotics, as determined by cryoelectron microscopy. Comparison with the ribosomes of other bacteria reveals several unique structural features at functionally important sites, including around the exit of the polypeptide tunnel and the periphery of the subunit interface. The structures also reveal the mode and site of interaction of these drugs with the ribosome. This work paves the way for the design of new inhibitors of translation to address infections caused by MDR A. baumannii. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6yhs.cif.gz | 1.9 MB | Display | PDBx/mmCIF format |
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PDB format | pdb6yhs.ent.gz | 1.4 MB | Display | PDB format |
PDBx/mmJSON format | 6yhs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6yhs_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 6yhs_full_validation.pdf.gz | 1.5 MB | Display | |
Data in XML | 6yhs_validation.xml.gz | 123.3 KB | Display | |
Data in CIF | 6yhs_validation.cif.gz | 219.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yh/6yhs ftp://data.pdbj.org/pub/pdb/validation_reports/yh/6yhs | HTTPS FTP |
-Related structure data
Related structure data | 10809MC 6ypuC 6ys5C 6ysiC 6yt9C 6ytfC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | |
EM raw data | EMPIAR-10406 (Title: Motion-corrected micrographs and extracted particle images of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with amikacin Data size: 177.7 Data #1: Motion-corrected micrographs of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with amikacin [micrographs - single frame] Data #2: Extracted particle images of the 70S ribosome from the human pathogen Acinetobacter baumannii in complex with amikacin [picked particles - multiframe - processed]) |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-RNA chain , 3 types, 4 molecules 1568
#1: RNA chain | Mass: 940330.125 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 (bacteria) References: GenBank: 1188467441 |
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#2: RNA chain | Mass: 37608.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 (bacteria) References: GenBank: 1560725104 |
#3: RNA chain | Mass: 24346.498 Da / Num. of mol.: 2 / Source method: isolated from a natural source Details: E. coli fMet-tRNA from PDB 5AFI fitted into EM density - represents a mixture of tRNAs Source: (natural) Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 (bacteria) |
+50S ribosomal protein ... , 27 types, 27 molecules ABCDEFGHIJKLMNOPQRSTUVXYZab
-Non-polymers , 2 types, 161 molecules
#31: Chemical | ChemComp-MG / #32: Chemical | ChemComp-ZN / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Acinetobacter baumannii ribosome-amikacin complex - 50S subunit Type: RIBOSOME / Entity ID: #1-#30 / Source: NATURAL |
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Source (natural) | Organism: Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841 (bacteria) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal magnification: 130000 X / Nominal defocus max: 2700 nm / Nominal defocus min: 800 nm |
Image recording | Average exposure time: 10 sec. / Electron dose: 58 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
Image scans | Movie frames/image: 50 |
-Processing
Software |
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EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||
Symmetry | Point symmetry: C1 (asymmetric) | ||||||||||||||||||||||||||||
3D reconstruction | Resolution: 2.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 51958 Details: Multi-body refinement was carried out in RELION 3.0 to obtain the final '50S subunit' reconstruction. The mask used for this procedure is deposited with this entry. Symmetry type: POINT | ||||||||||||||||||||||||||||
Atomic model building | Protocol: RIGID BODY FIT / Space: REAL / Target criteria: correlation coefficient | ||||||||||||||||||||||||||||
Atomic model building | 3D fitting-ID: 1 / Source name: PDB / Type: experimental model
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Refinement | Cross valid method: NONE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.82 Å2 | ||||||||||||||||||||||||||||
Refine LS restraints |
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