+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6xyw | ||||||
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タイトル | Structure of the plant mitochondrial ribosome | ||||||
要素 |
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キーワード | RIBOSOME / Mitochondria / Plant | ||||||
機能・相同性 | 機能・相同性情報 embryo sac central cell differentiation / endosperm cellularization / gametophyte development / pollen tube development / polar nucleus fusion / endosperm development / regulation of starch biosynthetic process / pollen germination / double fertilization forming a zygote and endosperm / embryo sac development ...embryo sac central cell differentiation / endosperm cellularization / gametophyte development / pollen tube development / polar nucleus fusion / endosperm development / regulation of starch biosynthetic process / pollen germination / double fertilization forming a zygote and endosperm / embryo sac development / 3-hydroxyisobutyryl-CoA hydrolase activity / karyogamy / pollen development / nucleus localization / regulation of programmed cell death / embryo development ending in seed dormancy / 加水分解酵素; エステル加水分解酵素; 3価のアルコールのエステル加水分解酵素 / cell communication / vacuole organization / plant-type vacuole / mitochondrial large ribosomal subunit / mitochondrial ribosome / mitochondrial small ribosomal subunit / mitochondrial membrane organization / mitochondrial translation / plastid / translation elongation factor activity / aminopeptidase activity / cytosolic ribosome / response to cold / chloroplast / trans-Golgi network / ribosomal small subunit biogenesis / large ribosomal subunit / regulation of translation / small ribosomal subunit / cytosolic small ribosomal subunit / regulation of gene expression / cytosolic large ribosomal subunit / transcription cis-regulatory region binding / rRNA binding / endosome / ribosome / structural constituent of ribosome / mitochondrial matrix / ribonucleoprotein complex / translation / copper ion binding / mRNA binding / Golgi apparatus / mitochondrion / RNA binding / nucleus / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | Arabidopsis thaliana (シロイヌナズナ) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.86 Å | ||||||
データ登録者 | Soufari, H. / Waltz, F. / Bochler, A. / Giege, P. / Hashem, Y. | ||||||
資金援助 | フランス, 1件
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引用 | ジャーナル: Nat Plants / 年: 2020 タイトル: Cryo-EM structure of the RNA-rich plant mitochondrial ribosome. 著者: Florent Waltz / Heddy Soufari / Anthony Bochler / Philippe Giegé / Yaser Hashem / 要旨: The vast majority of eukaryotic cells contain mitochondria, essential powerhouses and metabolic hubs. These organelles have a bacterial origin and were acquired during an early endosymbiosis event. ...The vast majority of eukaryotic cells contain mitochondria, essential powerhouses and metabolic hubs. These organelles have a bacterial origin and were acquired during an early endosymbiosis event. Mitochondria possess specialized gene expression systems composed of various molecular machines, including the mitochondrial ribosomes (mitoribosomes). Mitoribosomes are in charge of translating the few essential mRNAs still encoded by mitochondrial genomes. While chloroplast ribosomes strongly resemble those of bacteria, mitoribosomes have diverged significantly during evolution and present strikingly different structures across eukaryotic species. In contrast to animals and trypanosomatids, plant mitoribosomes have unusually expanded ribosomal RNAs and have conserved the short 5S rRNA, which is usually missing in mitoribosomes. We have previously characterized the composition of the plant mitoribosome, revealing a dozen plant-specific proteins in addition to the common conserved mitoribosomal proteins. In spite of the tremendous recent advances in the field, plant mitoribosomes remained elusive to high-resolution structural investigations and the plant-specific ribosomal features of unknown structures. Here, we present a cryo-electron microscopy study of the plant 78S mitoribosome from cauliflower at near-atomic resolution. We show that most of the plant-specific ribosomal proteins are pentatricopeptide repeat proteins (PPRs) that deeply interact with the plant-specific rRNA expansion segments. These additional rRNA segments and proteins reshape the overall structure of the plant mitochondrial ribosome, and we discuss their involvement in the membrane association and mRNA recruitment prior to translation initiation. Finally, our structure unveils an rRNA-constructive phase of mitoribosome evolution across eukaryotes. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6xyw.cif.gz | 4.2 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6xyw.ent.gz | 表示 | PDB形式 | |
PDBx/mmJSON形式 | 6xyw.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 6xyw_validation.pdf.gz | 1.8 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 6xyw_full_validation.pdf.gz | 2 MB | 表示 | |
XML形式データ | 6xyw_validation.xml.gz | 327.7 KB | 表示 | |
CIF形式データ | 6xyw_validation.cif.gz | 577.2 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/xy/6xyw ftp://data.pdbj.org/pub/pdb/validation_reports/xy/6xyw | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
+60S ribosomal protein ... , 3種, 3分子 AaAeAm
+タンパク質 , 49種, 49分子 AbAfAiAjAnAoApAsAtAxAyABACAEAFAGAIAJAKAMANAPBlBmBxBzBABPBFBd...
+50S ribosomal protein ... , 8種, 8分子 AcAdAkAqArAuAwAh
+Ribosomal protein ... , 11種, 11分子 ADAgAlAvAABbBfBaBcBsBk
+Mitochondrial ... , 2種, 2分子 AHBw
+Uncharacterized ... , 2種, 2分子 ALBu
+Pentatricopeptide repeat-containing protein ... , 3種, 3分子 AOAQBG
+タンパク質・ペプチド , 2種, 2分子 ARBO
+RNA鎖 , 3種, 3分子 132
+30S ribosomal protein ... , 3種, 3分子 BhBtBo
+40S ribosomal protein ... , 3種, 3分子 BiBrBg
+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: The large mitoribosomal subunit of the Plant mitoribosome タイプ: RIBOSOME Entity ID: #1, #10-#19, #2, #20-#29, #3, #30-#39, #4, #40-#49, #5, #50-#59, #6, #60-#69, #7, #70-#79, #8, #80-#89, #9 由来: NATURAL |
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分子量 | 実験値: NO |
由来(天然) | 生物種: Arabidopsis thaliana (シロイヌナズナ) |
緩衝液 | pH: 7.4 |
試料 | 濃度: 2 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 277 K |
-電子顕微鏡撮影
実験機器 | モデル: Talos Arctica / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TALOS ARCTICA |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 200 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD / Cs: 2.7 mm / C2レンズ絞り径: 30 µm |
撮影 | 平均露光時間: 1 sec. / 電子線照射量: 3 e/Å2 フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 撮影したグリッド数: 1 |
-解析
EMソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||
3次元再構成 | 解像度: 3.86 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 65280 / クラス平均像の数: 4 / 対称性のタイプ: POINT |