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基本情報
登録情報 | データベース: PDB / ID: 6g79 | |||||||||
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タイトル | Coupling specificity of heterotrimeric Go to the serotonin 5-HT1B receptor | |||||||||
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![]() | MEMBRANE PROTEIN / G-protein coupled receptor / 5-HT1B / Mini-Go / serotonin | |||||||||
機能・相同性 | ![]() guanyl nucleotide binding / voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels / negative regulation of gamma-aminobutyric acid secretion / serotonergic synapse / G protein-coupled serotonin receptor complex / adenylate cyclase-inhibiting serotonin receptor signaling pathway / dopamine neurotransmitter receptor activity, coupled via Gs / regulation of behavior / negative regulation of synaptic transmission, GABAergic / response to mineralocorticoid ...guanyl nucleotide binding / voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels / negative regulation of gamma-aminobutyric acid secretion / serotonergic synapse / G protein-coupled serotonin receptor complex / adenylate cyclase-inhibiting serotonin receptor signaling pathway / dopamine neurotransmitter receptor activity, coupled via Gs / regulation of behavior / negative regulation of synaptic transmission, GABAergic / response to mineralocorticoid / Serotonin receptors / negative regulation of serotonin secretion / drinking behavior / cellular response to temperature stimulus / bone remodeling / serotonin binding / negative regulation of synaptic transmission, glutamatergic / vasoconstriction / G protein-coupled serotonin receptor activity / : / G protein-coupled receptor internalization / vesicle docking involved in exocytosis / neurotransmitter receptor activity / G protein-coupled dopamine receptor signaling pathway / regulation of heart contraction / regulation of synaptic vesicle exocytosis / regulation of dopamine secretion / cellular response to alkaloid / calyx of Held / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / mu-type opioid receptor binding / corticotropin-releasing hormone receptor 1 binding / negative regulation of insulin secretion / G protein-coupled serotonin receptor binding / adenylate cyclase-activating adrenergic receptor signaling pathway / positive regulation of vascular associated smooth muscle cell proliferation / presynaptic modulation of chemical synaptic transmission / adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway / response to cocaine / locomotory behavior / muscle contraction / G-protein beta/gamma-subunit complex binding / Olfactory Signaling Pathway / Activation of the phototransduction cascade / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / G-protein activation / G protein-coupled acetylcholine receptor signaling pathway / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Prostacyclin signalling through prostacyclin receptor / Glucagon signaling in metabolic regulation / G beta:gamma signalling through CDC42 / ADP signalling through P2Y purinoceptor 12 / G beta:gamma signalling through BTK / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Sensory perception of sweet, bitter, and umami (glutamate) taste / photoreceptor disc membrane / Adrenaline,noradrenaline inhibits insulin secretion / Glucagon-type ligand receptors / Vasopressin regulates renal water homeostasis via Aquaporins / G alpha (z) signalling events / cellular response to catecholamine stimulus / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / sensory perception of taste / ADP signalling through P2Y purinoceptor 1 / adenylate cyclase-activating dopamine receptor signaling pathway / G beta:gamma signalling through PI3Kgamma / cellular response to prostaglandin E stimulus / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / GPER1 signaling / G-protein beta-subunit binding / Inactivation, recovery and regulation of the phototransduction cascade / heterotrimeric G-protein complex / G alpha (12/13) signalling events / extracellular vesicle / signaling receptor complex adaptor activity / cellular response to xenobiotic stimulus / Thrombin signalling through proteinase activated receptors (PARs) / GTPase binding / retina development in camera-type eye / presynaptic membrane / phospholipase C-activating G protein-coupled receptor signaling pathway / Ca2+ pathway / G alpha (i) signalling events / cell body / fibroblast proliferation / G alpha (s) signalling events / G alpha (q) signalling events / chemical synaptic transmission / response to ethanol / cell population proliferation / Ras protein signal transduction / Extra-nuclear estrogen signaling / G protein-coupled receptor signaling pathway / lysosomal membrane / GTPase activity 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.78 Å | |||||||||
![]() | Garcia-Nafria, J. / Nehme, R. / Edwards, P. / Tate, C.G. | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Cryo-EM structure of the serotonin 5-HT receptor coupled to heterotrimeric G. 著者: Javier García-Nafría / Rony Nehmé / Patricia C Edwards / Christopher G Tate / ![]() 要旨: G-protein-coupled receptors (GPCRs) form the largest family of receptors encoded by the human genome (around 800 genes). They transduce signals by coupling to a small number of heterotrimeric G ...G-protein-coupled receptors (GPCRs) form the largest family of receptors encoded by the human genome (around 800 genes). They transduce signals by coupling to a small number of heterotrimeric G proteins (16 genes encoding different α-subunits). Each human cell contains several GPCRs and G proteins. The structural determinants of coupling of G to four different GPCRs have been elucidated, but the molecular details of how the other G-protein classes couple to GPCRs are unknown. Here we present the cryo-electron microscopy structure of the serotonin 5-HT receptor (5-HTR) bound to the agonist donitriptan and coupled to an engineered G heterotrimer. In this complex, 5-HTR is in an active state; the intracellular domain of the receptor is in a similar conformation to that observed for the β-adrenoceptor (βAR) or the adenosine A receptor (AR) in complex with G. In contrast to the complexes with G, the gap between the receptor and the Gβ-subunit in the G-5-HTR complex precludes molecular contacts, and the interface between the Gα-subunit of G and the receptor is considerably smaller. These differences are likely to be caused by the differences in the interactions with the C terminus of the G α-subunit. The molecular variations between the interfaces of G and G in complex with GPCRs may contribute substantially to both the specificity of coupling and the kinetics of signalling. | |||||||||
履歴 |
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構造の表示
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構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 170.8 KB | 表示 | ![]() |
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-検証レポート
文書・要旨 | ![]() | 859.2 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 859.4 KB | 表示 | |
XML形式データ | ![]() | 30 KB | 表示 | |
CIF形式データ | ![]() | 45.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 4358MC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | |
電子顕微鏡画像生データ | ![]() Data size: 8.0 TB / Data #1: Unaligned movies [micrographs - multiframe] Data #2: Extracted particles [picked particles - multiframe - processed]) |
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リンク
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集合体
登録構造単位 | ![]()
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要素
#1: タンパク質 | 分子量: 37285.734 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
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#2: タンパク質 | 分子量: 7845.078 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
#3: タンパク質 | 分子量: 25155.727 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
#4: タンパク質 | 分子量: 41273.375 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() |
#5: 化合物 | ChemComp-EP5 / |
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 |
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由来(組換発現) |
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緩衝液 | pH: 7.5 | ||||||||||||||||||||||||||||||||||||
緩衝液成分 |
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試料 | 濃度: 2.2 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||||||||||||||||||||
試料支持 | グリッドの材料: GOLD / グリッドのサイズ: 300 divisions/in. / グリッドのタイプ: Quantifoil R1.2/1.3 | ||||||||||||||||||||||||||||||||||||
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 277.15 K |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / Cs: 2.7 mm / C2レンズ絞り径: 50 µm |
試料ホルダ | 凍結剤: NITROGEN 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
撮影 | 平均露光時間: 60 sec. / 電子線照射量: 30 e/Å2 / 検出モード: COUNTING フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 実像数: 5737 |
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解析
EMソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||||||
対称性 | 点対称性: C1 (非対称) | ||||||||||||||||||||||||||||||||||||||||
3次元再構成 | 解像度: 3.78 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 730118 / 対称性のタイプ: POINT |