ジャーナル: Nat Struct Mol Biol / 年: 2010 タイトル: Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus. 著者: Xiangan Liu / Qinfen Zhang / Kazuyoshi Murata / Matthew L Baker / Matthew B Sullivan / Caroline Fu / Matthew T Dougherty / Michael F Schmid / Marcia S Osburne / Sallie W Chisholm / Wah Chiu / 要旨: Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of ...Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatches are accommodated among five of the gene products at the portal vertex. Reconstructions of infectious and empty particles show a conformational change of the 'valve' density in the nozzle, an orientation difference in the tail fibers, a disordering of the C terminus of the portal protein and the disappearance of the core proteins. In addition, cryo-electron tomography of P-SSP7 infecting Prochlorococcus showed the same tail-fiber conformation as that in empty particles. Our observations suggest a mechanism whereby, upon binding to the host cell, the tail fibers induce a cascade of structural alterations of the portal vertex complex that triggers DNA release.
履歴
登録
2010年4月30日
登録サイト: PDBE / 処理サイト: PDBE
改定 1.0
2010年6月16日
Provider: repository / タイプ: Initial release
改定 1.1
2013年3月20日
Group: Other / Refinement description / Version format compliance
改定 1.2
2018年10月3日
Group: Data collection / カテゴリ: em_software / Item: _em_software.image_processing_id
改定 1.3
2019年10月23日
Group: Data collection / Other / カテゴリ: cell / Item: _cell.Z_PDB
A: T7-LIKE CAPSID PROTEIN B: T7-LIKE CAPSID PROTEIN C: T7-LIKE CAPSID PROTEIN D: T7-LIKE CAPSID PROTEIN E: T7-LIKE CAPSID PROTEIN F: T7-LIKE CAPSID PROTEIN G: T7-LIKE CAPSID PROTEIN
A: T7-LIKE CAPSID PROTEIN B: T7-LIKE CAPSID PROTEIN C: T7-LIKE CAPSID PROTEIN D: T7-LIKE CAPSID PROTEIN E: T7-LIKE CAPSID PROTEIN F: T7-LIKE CAPSID PROTEIN G: T7-LIKE CAPSID PROTEIN
A: T7-LIKE CAPSID PROTEIN B: T7-LIKE CAPSID PROTEIN C: T7-LIKE CAPSID PROTEIN D: T7-LIKE CAPSID PROTEIN E: T7-LIKE CAPSID PROTEIN F: T7-LIKE CAPSID PROTEIN G: T7-LIKE CAPSID PROTEIN
x 5
icosahedral pentamer
1.38 MDa, 35 ポリマー
分子量 (理論値)
分子数
合計 (水以外)
1,382,297
35
ポリマ-
1,382,297
35
非ポリマー
0
0
水
0
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
A: T7-LIKE CAPSID PROTEIN B: T7-LIKE CAPSID PROTEIN C: T7-LIKE CAPSID PROTEIN D: T7-LIKE CAPSID PROTEIN E: T7-LIKE CAPSID PROTEIN F: T7-LIKE CAPSID PROTEIN G: T7-LIKE CAPSID PROTEIN
モード: BRIGHT FIELD / 倍率(公称値): 60000 X / 最大 デフォーカス(公称値): 3000 nm / 最小 デフォーカス(公称値): 600 nm / Cs: 4.1 mm
試料ホルダ
温度: 101 K
撮影
電子線照射量: 20 e/Å2 / フィルム・検出器のモデル: KODAK SO-163 FILM
画像スキャン
デジタル画像の数: 1059
放射波長
相対比: 1
-
解析
EMソフトウェア
名称: MPSA / カテゴリ: 3次元再構成
CTF補正
詳細: INDIVIDUAL MICROGRAPH
対称性
点対称性: I (正20面体型対称)
3次元再構成
手法: CROSS-COMMON LINES / 解像度: 4.6 Å / 粒子像の数: 36000 / ピクセルサイズ(公称値): 1.27 Å / ピクセルサイズ(実測値): 1.17 Å / 倍率補正: 2D CRYSTAL 詳細: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1713. 対称性のタイプ: POINT