登録情報 データベース : EMDB / ID : EMD-7027 構造の表示 ダウンロードとリンクタイトル Apo KLP10A in complex with a microtubule マップデータMap locally filtered and sharpened with a B-factor of -130 A^2 詳細 試料複合体 : apo-KLP10A neck-motor in complex with a microtubule複合体 : apo-KLP10A neck-motorタンパク質・ペプチド : Kinesin-like protein Klp10A細胞器官・細胞要素 : microtubuleタンパク質・ペプチド : Tubulin alpha-1B chainタンパク質・ペプチド : Tubulin beta chainリガンド : GUANOSINE-5'-TRIPHOSPHATEリガンド : MAGNESIUM IONリガンド : GUANOSINE-5'-DIPHOSPHATEリガンド : TAXOL 詳細 キーワード kinesin 13 / microtubule / tubulin / depolymerization / MOTOR PROTEIN-STRUCTURAL PROTEIN complex機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
establishment of mitotic spindle asymmetry / establishment of meiotic spindle orientation / plus-end specific microtubule depolymerization / asymmetric protein localization involved in cell fate determination / cortical microtubule / meiotic spindle pole / mitotic spindle astral microtubule / centriole assembly / COPI-dependent Golgi-to-ER retrograde traffic / Kinesins ... establishment of mitotic spindle asymmetry / establishment of meiotic spindle orientation / plus-end specific microtubule depolymerization / asymmetric protein localization involved in cell fate determination / cortical microtubule / meiotic spindle pole / mitotic spindle astral microtubule / centriole assembly / COPI-dependent Golgi-to-ER retrograde traffic / Kinesins / mitotic chromosome movement towards spindle pole / kinetochore microtubule / meiotic spindle organization / spindle assembly involved in female meiosis I / non-motile cilium assembly / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Carboxyterminal post-translational modifications of tubulin / RHOH GTPase cycle / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / PKR-mediated signaling / The role of GTSE1 in G2/M progression after G2 checkpoint / Aggrephagy / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Separation of Sister Chromatids / plus-end-directed microtubule motor activity / RHO GTPases activate IQGAPs / RHO GTPases Activate Formins / meiotic spindle / Recruitment of NuMA to mitotic centrosomes / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / COPI-mediated anterograde transport / microtubule depolymerization / microtubule motor activity / kinesin complex / spindle organization / microtubule-based movement / mitotic spindle pole / cytoskeletal motor activity / centrosome duplication / chromosome, centromeric region / mitotic spindle organization / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / structural constituent of cytoskeleton / spindle pole / microtubule cytoskeleton organization / spindle / microtubule cytoskeleton / mitotic cell cycle / microtubule binding / microtubule / cell division / GTPase activity / centrosome / GTP binding / ATP hydrolysis activity / ATP binding / metal ion binding / cytoplasm 類似検索 - 分子機能 Kinesin-like protein / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain / Kinesin motor domain profile. / Kinesin motor, catalytic domain. ATPase. / Kinesin motor domain / Kinesin motor domain superfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin ... Kinesin-like protein / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain / Kinesin motor domain profile. / Kinesin motor, catalytic domain. ATPase. / Kinesin motor domain / Kinesin motor domain superfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性 Tubulin beta chain / Tubulin beta chain / Tubulin alpha-1B chain / Kinesin-like protein Klp10A 類似検索 - 構成要素生物種 Drosophila melanogaster (キイロショウジョウバエ) / Sus scrofa (ブタ)手法 らせん対称体再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.78 Å 詳細 データ登録者Benoit MPMH / Asenjo AB 資金援助 米国, 1件 詳細 詳細を隠すOrganization Grant number 国 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM113164 米国
引用ジャーナル : Nat Commun / 年 : 2018タイトル : Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s.著者 : Matthieu P M H Benoit / Ana B Asenjo / Hernando Sosa / 要旨 : Kinesin-13s constitute a distinct group within the kinesin superfamily of motor proteins that promote microtubule depolymerization and lack motile activity. The molecular mechanism by which kinesin- ... Kinesin-13s constitute a distinct group within the kinesin superfamily of motor proteins that promote microtubule depolymerization and lack motile activity. The molecular mechanism by which kinesin-13s depolymerize microtubules and are adapted to perform a seemingly very different activity from other kinesins is still unclear. To address this issue, here we report the near atomic resolution cryo-electron microscopy (cryo-EM) structures of Drosophila melanogaster kinesin-13 KLP10A protein constructs bound to curved or straight tubulin in different nucleotide states. These structures show how nucleotide induced conformational changes near the catalytic site are coupled with movement of the kinesin-13-specific loop-2 to induce tubulin curvature leading to microtubule depolymerization. The data highlight a modular structure that allows similar kinesin core motor-domains to be used for different functions, such as motility or microtubule depolymerization. 履歴 登録 2017年9月14日 - ヘッダ(付随情報) 公開 2018年2月14日 - マップ公開 2018年5月2日 - 更新 2024年3月13日 - 現状 2024年3月13日 処理サイト : RCSB / 状態 : 公開
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