|Entry||Database: EMDB / ID: 1701|
|Title||The molecular mechanism of the multi-tasking kinesin-8 motor|
|Keywords||Molecular motor / kinesin 8 / ruby-helix|
|Sample||Microtubule complexed with motor domain of Kif18A (no nucleotide state)|
|Source||Homo sapiens / human / |
Bos taurus / mammal / Cattle /
|Map data||10A reconstruction of a 15pf microtubule decorated with the motor domain of Kif18A (kinesin 8) in the Apo-state|
|Method||helical reconstruction, at 10.1 Å resolution|
|Authors||Peters C / Brejc K / Belmont L / Bodey A / Lee Y / Yu M / Ramchandani S / Guo J / Lichtsteiner S / Wood KW / Sakowicz R / Hartman J / Moores C|
|Citation||EMBO J., 2010, 29, 3437-3447|
|Date||Deposition: Feb 9, 2010 / Header (metadata) release: Mar 10, 2010 / Map release: Oct 1, 2010 / Last update: Sep 9, 2011|
Downloads & links
|File||emd_1701.map.gz (map file in CCP4 format, 128001 KB)|
|Projections & slices|
Images are generated by Spider package.
|Voxel size||X=Y=Z: 1.4 Å|
CCP4 map header:
-Entire Microtubule complexed with motor domain of Kif18A (no nucleotide ...
|Entire||Name: Microtubule complexed with motor domain of Kif18A (no nucleotide state)|
Number of components: 3
-Component #1: protein, Motor domain of Kif18A
|Protein||Name: Motor domain of Kif18A / a.k.a: Motor domain of Kif18A / Details: Contains the N-terminal 355 aa / Recombinant expression: Yes|
|Source||Species: Homo sapiens / human /|
|Source (engineered)||Expression System: Escherichia coli rosetta de3 / bacteria / image: Escherichia coli|
|Source (natural)||Location in cell: Cytoplasm|
-Component #2: protein, Alpha tubulin
|Protein||Name: Alpha tubulin / a.k.a: Alpha tubulin / Recombinant expression: No|
|Source||Species: Bos taurus / mammal / Cattle /|
|Source (natural)||Location in cell: Cytoplasm / Organ or tissue: brain|
-Component #3: protein, Beta tubulin
|Protein||Name: Beta tubulin / a.k.a: Beta tubulin / Recombinant expression: No|
|Source||Species: Bos taurus / mammal / Cattle /|
|Source (natural)||Location in cell: Cytoplasm / Organ or tissue: Brain|
|Helical parameters||Hand: RIGHT HANDED|
|Sample solution||Buffer solution: 80mM PIPES, 150mM NaCl, 7mM MgCl2, 1mM EGTA, 1mM beta-mercaptoethanol|
|Support film||400 mesh copper grid|
|Vitrification||Instrument: HOMEMADE PLUNGER / Cryogen name: ETHANE / Method: Blotted for 1 sec before plunging|
Time resolved state: Vitrified after incubating with Apyrase
Details: Vitrification instrument: Manual plunger
-Electron microscopy imaging
Model: Tecnai F20 / Image courtesy: FEI Company
|Imaging||Microscope: FEI TECNAI F20 / Details: Low dose|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Electron dose: 10 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Magnification: 50000 X (nominal), 50000 X (calibrated)|
Astigmatism: Objective lense stigmatism was corrected at 150,000 times magnification
Cs: 2 mm / Imaging mode: OTHER / Defocus: 920 - 3300 nm
|Specimen Holder||Holder: cryo-holder / Model: GATAN LIQUID NITROGEN|
|Camera||Detector: KODAK SO-163 FILM|
|Image acquisition||Number of digital images: 50 / Scanner: ZEISS SCAI / Sampling size: 7 microns / Bit depth: 12|
|Processing||Method: helical reconstruction|
|3D reconstruction||Algorithm: Helical processing / Software: Ruby-Helix / CTF correction: Phase flipping, Wiener|
Details: Fourier Bessel Synthesis using data from 25 microtubules (36000 asymmetric units)
Resolution: 10.1 Å / Resolution method: FSC 0.5
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