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- EMDB-66682: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Ch... -

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Basic information

Entry
Database: EMDB / ID: EMD-66682
TitleCryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Rrp12-A1
Map datalocal resolution filtered map using Relion
Sample
  • Complex: pre-40S ribosome, state Rrp12-A1
    • Protein or peptide: x 30 types
    • RNA: x 1 types
  • Ligand: x 3 types
Keywords90S pre-ribosome / Rrp12 / Enp1 / ribosome biogenesis / RIBOSOME
Function / homology
Function and homology information


rRNA (guanine-N7)-methylation / rRNA (guanine) methyltransferase activity / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / U3 snoRNA binding / snoRNA binding / preribosome, small subunit precursor / 90S preribosome / ribosomal small subunit export from nucleus / cytosolic ribosome / methyltransferase activity ...rRNA (guanine-N7)-methylation / rRNA (guanine) methyltransferase activity / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / U3 snoRNA binding / snoRNA binding / preribosome, small subunit precursor / 90S preribosome / ribosomal small subunit export from nucleus / cytosolic ribosome / methyltransferase activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / protein tag activity / rRNA processing / ribosomal small subunit assembly / ribosome biogenesis / ribosomal small subunit biogenesis / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / methylation / cytoplasmic translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / GTPase activity / GTP binding / nucleolus / RNA binding / nucleus / cytoplasm / cytosol
Similarity search - Function
Uncharacterised domain NUC173 / : / : / Ribosomal RNA-processing protein 12, HEAT repeats / RRP12-like, N-terminal HEAT repeats / 18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23, C-terminal / 18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23-like / Methyltransferase involved in Williams-Beuren syndrome / Low temperature viability protein Ltv1 / Low temperature viability protein ...Uncharacterised domain NUC173 / : / : / Ribosomal RNA-processing protein 12, HEAT repeats / RRP12-like, N-terminal HEAT repeats / 18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23, C-terminal / 18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23-like / Methyltransferase involved in Williams-Beuren syndrome / Low temperature viability protein Ltv1 / Low temperature viability protein / Methyltransferase type 11 / Tsr1, G-like domain / Methyltransferase domain / Bystin / Bystin / : / : / Eukaryotic type KH-domain (KH-domain type I) / Ribosome biogenesis protein BMS1/TSR1, C-terminal / AARP2CN / Bms1/Tsr1-type G domain / Ribosome biogenesis protein Bms1/Tsr1 / 40S ribosome biogenesis protein Tsr1 and BMS1 C-terminal / AARP2CN (NUC121) domain / Bms1-type guanine nucleotide-binding (G) domain profile. / AARP2CN (NUC121) domain / Protein of unknown function (DUF663) / K Homology domain, type 1 superfamily / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / S27a-like superfamily / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S8e subdomain, eukaryotes / : / Ribosomal protein S7e signature. / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S19A/S15e / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / Ribosomal protein S6, eukaryotic / 40S ribosomal protein S1/3, eukaryotes / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / : / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S28e conserved site / Ribosomal protein S28e signature. / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S6e signature. / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S8e / Ribosomal protein S27 / Ribosomal protein S24e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S24e / Ribosomal protein S27 / Ribosomal protein S28e / Ribosomal protein S28e / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal S3Ae family / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / 50S ribosomal protein L30e-like / K Homology domain / K homology RNA-binding domain / :
Similarity search - Domain/homology
40S ribosomal protein s23-like protein / 40S ribosomal protein S6 / 40S ribosomal protein S8 / Bms1-type G domain-containing protein / 40S ribosomal protein S13-like protein / Uncharacterized protein / 40S ribosomal protein s15-like protein / 40S ribosomal protein s9-like protein / 40S ribosomal protein S4 / Ribosomal protein s27-like protein ...40S ribosomal protein s23-like protein / 40S ribosomal protein S6 / 40S ribosomal protein S8 / Bms1-type G domain-containing protein / 40S ribosomal protein S13-like protein / Uncharacterized protein / 40S ribosomal protein s15-like protein / 40S ribosomal protein s9-like protein / 40S ribosomal protein S4 / Ribosomal protein s27-like protein / 40S ribosomal protein S25 / 40S ribosomal protein s5-like protein / 40S ribosomal protein S27a-like protein / Pre-rRNA-processing protein PNO1 / Bystin-like protein / Putative ribosomal protein / Small ribosomal subunit protein eS1 / 40S ribosomal protein S7 / 40S ribosomal protein S28-like protein / 40S ribosomal protein S16-like protein / Methyltransferase-like protein / Low-temperature viability protein / 40S ribosomal protein S11-like protein / 40S ribosomal protein S19-like protein / 40S ribosomal protein S14-like protein / 40S ribosomal protein S12 / 40S ribosomal protein S22-like protein / Small ribosomal subunit protein eS24
Similarity search - Component
Biological speciesThermochaetoides thermophila (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsLau B / Li Y / Zhu J / Fischer P / Hong X / Yuan R / Beckmann R / Hurt E / Cheng J
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Cell Rep / Year: 2026
Title: Nucleoplasmic checkpoint of the 40S ribosomal decoding center maturation.
Authors: Benjamin Lau / Yi Li / Jingyi Zhu / Xianwen Ye / Paulina Fischer / Xiaying Hong / Rui Yuan / Roland Beckmann / Ed Hurt / Jingdong Cheng /
Abstract: The decoding center (DC) is a key ribosomal structure for accurate translation, assembled in a multi-step process that starts on nucleolar pre-ribosomes and ends in the cytoplasm. While late ...The decoding center (DC) is a key ribosomal structure for accurate translation, assembled in a multi-step process that starts on nucleolar pre-ribosomes and ends in the cytoplasm. While late cytoplasmic steps and their checkpoint mechanisms are well characterized, the regulation of early nucleoplasmic DC assembly is unclear. Here, we show that the essential assembly factor Rrp12 plays a central coordinating role. Using Chaetomium thermophilum and cryo-electron microscopy analyses of fifteen pre-40S intermediates, we demonstrate that Rrp12 C terminus truncation: (1) inhibits release of the Utp14-Dhr1 pair, (2) displaces Tsr1, (3) promotes premature stabilization of h28, and (4) prevents h44 formation. These defects impair final 18S rRNA processing and prematurely activate the quality control kinase Rio1. Our results reveal a nucleoplasmic checkpoint during DC formation and establish Rrp12 as a critical regulator ensuring accurate assembly and orderly ribosome maturation.
History
DepositionOct 22, 2025-
Header (metadata) releaseJun 24, 2026-
Map releaseJun 24, 2026-
UpdateJun 24, 2026-
Current statusJun 24, 2026Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_66682.png

Downloads & links

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Map

FileDownload / File: emd_66682.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationlocal resolution filtered map using Relion
Projections & slices

Image control

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AxesZ (Sec.)Y (Row.)X (Col.)
1.15 Å/pix.
x 360 pix.
= 412.56 Å		1.15 Å/pix.
x 360 pix.
= 412.56 Å		1.15 Å/pix.
x 360 pix.
= 412.56 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.146 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.26426908 - 0.47864985
Average (Standard dev.)0.00035942037 (±0.008061986)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 412.56 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: multibody refined map focusing on 40S head and...

Fileemd_66682_additional_1.map
Annotationmultibody refined map focusing on 40S head and local resolution filtered using deepEMhancer
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Additional map: consensus refined map without post processing

Fileemd_66682_additional_2.map
Annotationconsensus refined map without post processing
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Additional map: multibody refined map focusing on 40S body and...

Fileemd_66682_additional_3.map
Annotationmultibody refined map focusing on 40S body and local resolution filtered using deepEMhancer
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Additional map: local resolution filtered map using deepEMhancer

Fileemd_66682_additional_4.map
Annotationlocal resolution filtered map using deepEMhancer
Projections & Slices
AxesZYX

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Half map: half map

Fileemd_66682_half_map_1.map
Annotationhalf map
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Half map: half map

Fileemd_66682_half_map_2.map
Annotationhalf map
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Sample components

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Entire : pre-40S ribosome, state Rrp12-A1

EntireName: pre-40S ribosome, state Rrp12-A1
Components
  • Complex: pre-40S ribosome, state Rrp12-A1
    • Protein or peptide: Small ribosomal subunit protein eS1
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: 40S ribosomal protein s5-like protein
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein s9-like protein
    • Protein or peptide: 40S ribosomal protein S11-like protein
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S13-like protein
    • Protein or peptide: 40S ribosomal protein S14-like protein
    • Protein or peptide: 40S ribosomal protein s15-like protein
    • Protein or peptide: 40S ribosomal protein S16-like protein
    • Protein or peptide: Putative ribosomal protein
    • Protein or peptide: 40S ribosomal protein S19-like protein
    • Protein or peptide: 40S ribosomal protein S22-like protein
    • Protein or peptide: 40S ribosomal protein s23-like protein
    • Protein or peptide: Small ribosomal subunit protein eS24
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: Ribosomal protein s27-like protein
    • Protein or peptide: 40S ribosomal protein S28-like protein
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: 40S ribosomal protein S27a-like protein
    • RNA: 18S rRNA
    • Protein or peptide: Trm112
    • Protein or peptide: Methyltransferase-like protein
    • Protein or peptide: Bms1-type G domain-containing protein
    • Protein or peptide: Pre-rRNA-processing protein PNO1
    • Protein or peptide: Bystin-like protein
    • Protein or peptide: Putative low-temperature viability protein
    • Protein or peptide: Ribosomal RNA-processing protein 12-like conserved domain-containing protein
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: water

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Supramolecule #1: pre-40S ribosome, state Rrp12-A1

SupramoleculeName: pre-40S ribosome, state Rrp12-A1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#31
Source (natural)Organism: Thermochaetoides thermophila (fungus)

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Macromolecule #1: Small ribosomal subunit protein eS1

MacromoleculeName: Small ribosomal subunit protein eS1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 29.245158 KDa
SequenceString: MAVGKNKRLS KGKKGLKKKV QDPFTRKDWY NIKAPAPFAV RDVGKTLVNR TTGLKNANDA LKGRIFEVCL ADLQKDEDHA FRKIKLRVD EVQGKNCLTN FHGLDFTTDK LRSLVRKWQT LIEANVTVTT TDHYLLRLFA IAFTKRRPNQ IKKTTYAQSS Q IRAIRRKM ...String:
MAVGKNKRLS KGKKGLKKKV QDPFTRKDWY NIKAPAPFAV RDVGKTLVNR TTGLKNANDA LKGRIFEVCL ADLQKDEDHA FRKIKLRVD EVQGKNCLTN FHGLDFTTDK LRSLVRKWQT LIEANVTVTT TDHYLLRLFA IAFTKRRPNQ IKKTTYAQSS Q IRAIRRKM VEIIQREAAS CTLHQLVSKL IPEVIGREIE KATQGIYPLQ NVHIRKVKLL KQPKFDLGAL MALHGESSEE AG QKVEREF REQVLESV

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #2: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 29.800764 KDa
SequenceString: MARGPKKHQK RLSAPSHWLL DKLSGAYAPR PSTGPHKLRD CMPLIVFVRN RLKYALNYRE TKAIMMQRLV KVDGKVRTDI TYPAGFMDV ITIEKTGENF RLIYDTKGRF TVHRITDEEA KYKLGKVKRV QLGRGGVPFL VTHDARTIRY PDPLIKVNDT V KIDLETGK ...String:
MARGPKKHQK RLSAPSHWLL DKLSGAYAPR PSTGPHKLRD CMPLIVFVRN RLKYALNYRE TKAIMMQRLV KVDGKVRTDI TYPAGFMDV ITIEKTGENF RLIYDTKGRF TVHRITDEEA KYKLGKVKRV QLGRGGVPFL VTHDARTIRY PDPLIKVNDT V KIDLETGK ITDFIKFDTG ALAMITGGRN MGRVGVITHR ERHDGGFGIV HLKDALDNTF ATRESNVFVI GSEKPWISLP KG KGVKLTI AEERDQRRAR ALAAAGH

UniProtKB: 40S ribosomal protein S4

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Macromolecule #3: 40S ribosomal protein s5-like protein

MacromoleculeName: 40S ribosomal protein s5-like protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 23.679225 KDa
SequenceString: MSEGEVEVAQ PQYDVLPKEV LAEVGSVKLF NRWSYDDIEI RDISLTDYIQ IRAPVYIPHS AGRYAVKRFR KANCPIIERL TNSLMMHGR NNGKKLMAVR IVAHAFEIIH LMTDQNPIQV AVDAIVNCGP REDSTRIGSA GTVRRQAVDV SPLRRVNQAI A LLTTGARE ...String:
MSEGEVEVAQ PQYDVLPKEV LAEVGSVKLF NRWSYDDIEI RDISLTDYIQ IRAPVYIPHS AGRYAVKRFR KANCPIIERL TNSLMMHGR NNGKKLMAVR IVAHAFEIIH LMTDQNPIQV AVDAIVNCGP REDSTRIGSA GTVRRQAVDV SPLRRVNQAI A LLTTGARE AAFRNVKTIA ECLAEELINA AKGSSNSYAI KKKDELERVA KSNR

UniProtKB: 40S ribosomal protein s5-like protein

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Macromolecule #4: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 27.490139 KDa
SequenceString: MKLNISHPAN GSQKLIEVED ERKLRHFYDK RMGAEVAGDP LGPEWKGYIL RITGGNDKQG FPMKQGVIAP NRVRLLLSEG HSCYRPRRD GERKRKSVRG CIVGPDLSVL ALSIVKQGEQ DIPGLTDVVH PKRLGPKRAT KIRRFFSLSK DDDVRKYVIR R EVQPKGEG ...String:
MKLNISHPAN GSQKLIEVED ERKLRHFYDK RMGAEVAGDP LGPEWKGYIL RITGGNDKQG FPMKQGVIAP NRVRLLLSEG HSCYRPRRD GERKRKSVRG CIVGPDLSVL ALSIVKQGEQ DIPGLTDVVH PKRLGPKRAT KIRRFFSLSK DDDVRKYVIR R EVQPKGEG KKPYTKAPRI QRLVTPQRLQ HKRHRIALKR RQQEKVKEEA AEYAQILAKR VAEAKAQKAD LRKRRASSLH K

UniProtKB: 40S ribosomal protein S6

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Macromolecule #5: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 23.08465 KDa
SequenceString: MSAPSLNKIA ANSPSRQNPS DLERAIAGAL YDLETNTADL KAALRPLQFV SAREIEVGHG KKAIVIFVPV PALQGFHRVQ QRLTRELEK KFSDRHVLIL AARRILPKPK RSARSRNTLK QKRPRSRTLT AVHDAILTDL VYPVEIVGKR LRTKEDGSKV L KVILDEKE ...String:
MSAPSLNKIA ANSPSRQNPS DLERAIAGAL YDLETNTADL KAALRPLQFV SAREIEVGHG KKAIVIFVPV PALQGFHRVQ QRLTRELEK KFSDRHVLIL AARRILPKPK RSARSRNTLK QKRPRSRTLT AVHDAILTDL VYPVEIVGKR LRTKEDGSKV L KVILDEKE RGGVDYRLDT YSEVYRRLTG RNVTFEFPQT TITDY

UniProtKB: 40S ribosomal protein S7

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Macromolecule #6: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 23.102416 KDa
SequenceString: MGISRDSRHK RSHTGAKRAF YRKKRAFELG RQPANTRIGP KRIHIVRTRG GNHKYRALRL DSGNFAWGSE GCTRKTRIIG VVYHPSNNE LVRTNTLTKS AVVQIDAAPF RQWYEAHYGQ PLGRRRQQKQ GQVVEEVKKS KSVEKKQAAR FAAHGKVDPA L EKQFEAGR ...String:
MGISRDSRHK RSHTGAKRAF YRKKRAFELG RQPANTRIGP KRIHIVRTRG GNHKYRALRL DSGNFAWGSE GCTRKTRIIG VVYHPSNNE LVRTNTLTKS AVVQIDAAPF RQWYEAHYGQ PLGRRRQQKQ GQVVEEVKKS KSVEKKQAAR FAAHGKVDPA L EKQFEAGR LYAIISSRPG QSGRADGYIL EGEELAFYQR KLHK

UniProtKB: 40S ribosomal protein S8

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Macromolecule #7: 40S ribosomal protein s9-like protein

MacromoleculeName: 40S ribosomal protein s9-like protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 22.029836 KDa
SequenceString:
MAPRKYSKTY KVPRRPYEAA RLDSELKLVG EYGLRNKREV WRVLLTLSKI RRAARILLTL DEKDPKRLFE GNALIRRLVR IGVLDESRM KLDYVLALKA EDFLERRLQT LVYKLGLAKS IHHARVLIRQ RHIRVGKQIV NVPSFMVRLD SQKHIDFALT S PFGGGRPG RVRRKKAKAA EGGEGGEEEE EE

UniProtKB: 40S ribosomal protein s9-like protein

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Macromolecule #8: 40S ribosomal protein S11-like protein

MacromoleculeName: 40S ribosomal protein S11-like protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 18.698182 KDa
SequenceString:
MATELTVQSE RAFLKQPHIF LNSKVKVKST RPGKGGRRWY KDVGLGFKTP KTAIEGHYID KKCPFTGMVS IRGRILTGRV VSTKMHRTI IIRREYLHYI PKYNRYEKRH KNLAAHVSPA FRVEEGDMVV VGQCRPLSKT VRFNVLRVLP RTGKSVKKFQ K F

UniProtKB: 40S ribosomal protein S11-like protein

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Macromolecule #9: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 15.961293 KDa
SequenceString:
MSDVEETTAP QEVEVSAEAG KGQMSVLDAL KGVLKLSLMH DGLARGLREA SKALDRRQAH MCVLNESCEE EAYKKLVIAL CQEHKIPLI KVPDGKQLGE WAGLCVLDRE GNARKVVNCS CVVVKDWGEE SQERTVLLNY FQQEA

UniProtKB: 40S ribosomal protein S12

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Macromolecule #10: 40S ribosomal protein S13-like protein

MacromoleculeName: 40S ribosomal protein S13-like protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 16.912891 KDa
SequenceString:
MGRLHSKGKG ISASAIPYSR NPPAWLKTTP EQVVEQICKL ARKGATPSQI GVILRDSHGI AQVKVVTGNK ILRILKSNGL APDIPEDLY FLIKKAVAVR KHLERNRKDK DSKFRLILIE SRIHRLARYY KTVGVLPPTW KYESSTASTL VA

UniProtKB: 40S ribosomal protein S13-like protein

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Macromolecule #11: 40S ribosomal protein S14-like protein

MacromoleculeName: 40S ribosomal protein S14-like protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 16.071464 KDa
SequenceString:
MPPKKTTRPA QENISLGPQV REGELVFGVA RIFASFNDTF VHVTDLSGRE TICRVTGGMK VKADRDESSP YAAMLAAQDV AARCKELGI TALHIKIRAT GGNGTKTPGP GAQSALRALA RSGMKIGRIE DVTPTPSDST RRKGGRRGRR L

UniProtKB: 40S ribosomal protein S14-like protein

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Macromolecule #12: 40S ribosomal protein s15-like protein

MacromoleculeName: 40S ribosomal protein s15-like protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 17.601604 KDa
SequenceString:
MADTEYNAEE AAELKKKRTF RKFSYRGIDL EALLDLDSDE LRNVLHARAR RKINRGLKRR PLGLIKKLRK AKQEAKPNEK PELVKTHLR DMIVVPEMIG SVIGIYSGKE FNQVEIKPEM VGHYLGEFSI TYKPVKHGRP GIGATHSSRF IPLK

UniProtKB: 40S ribosomal protein s15-like protein

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Macromolecule #13: 40S ribosomal protein S16-like protein

MacromoleculeName: 40S ribosomal protein S16-like protein / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 15.962771 KDa
SequenceString:
MASVQAVQVF GKKKNATAVA RCVQGKGLIK VNGKPLKLFA PEILRAKLYE PILILGTDKF ADVDIRIRVA GGGHTSQVYA VRQAIAKSI VAYYAKYVDE HSKNLLKQEL IQFDRSLLVA DPRRCEPKKF GGRGARARFQ KSYR

UniProtKB: 40S ribosomal protein S16-like protein

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Macromolecule #14: Putative ribosomal protein

MacromoleculeName: Putative ribosomal protein / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 17.826707 KDa
SequenceString:
MSLVSGEKTN FNHILRLLNT NVDGKQKVVY ALTQIKGVGR RYSNLVCKKA DVDLKKRAGE LTSEELERLV TIIQNPLAYK IPTWFLNRQ RDIVDGKDSQ ILANGVDSKL REDLERLKKI RAHRGLRHYW GLRVRGQHTK TTGRRGRTVG VSKKKGG

UniProtKB: Putative ribosomal protein

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Macromolecule #15: 40S ribosomal protein S19-like protein

MacromoleculeName: 40S ribosomal protein S19-like protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 17.151332 KDa
SequenceString:
MPGGVTVRDV DAHKFVAAYA AFLKRQGKLP VPGWVDTVKT GNAKELPPQD IDWWYIRAAS VARHVYLRKT VGVGRLRKVH GSAKNRGTR PSHHVDASGS VDRKIMQALE KIQVLEKDEE RGGRRITQQG QRDLDRIAQT VAEADQESEA EEEE

UniProtKB: 40S ribosomal protein S19-like protein

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Macromolecule #16: 40S ribosomal protein S22-like protein

MacromoleculeName: 40S ribosomal protein S22-like protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 14.905471 KDa
SequenceString:
MVRTSVLHDA LNSINNAEKM GKRQVMIRPS SKVIVKFLQV MQRHGYIGEF EEVDNHRSGK IVVQLNGRLN KCGVISPRYN VRLAELEKW VTKLLPARQF GYVILTTSAG IMDHEEARRK HVAGKIIGFF Y

UniProtKB: 40S ribosomal protein S22-like protein

+
Macromolecule #17: 40S ribosomal protein s23-like protein

MacromoleculeName: 40S ribosomal protein s23-like protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 15.934653 KDa
SequenceString:
MSGGKPRGLN AARKLRNNRR EQRWADLQYK KRALGTAYKS SPFGGSSHAK GIVLEKVGVE AKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VGLLALWKEK KEKPRS

UniProtKB: 40S ribosomal protein s23-like protein

+
Macromolecule #18: Small ribosomal subunit protein eS24

MacromoleculeName: Small ribosomal subunit protein eS24 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 15.535286 KDa
SequenceString:
MADTDSPVTL RTRKFIRNPL LSRKQMVVDI LHPGRPNISK DELREKLATM YKAQKDQVSV FGLRTQYGGG KTTGFALIYD SPEALKKFE PRYRLVRAGL AAKVEKASRQ QRKQRKNRLK TLRGTAKVKG AKAKKEK

UniProtKB: Small ribosomal subunit protein eS24

+
Macromolecule #19: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 10.932943 KDa
SequenceString:
MAPAAQSGAK KQKKKWSKGK VKDKAQHSVI LDKATSDKLY KDVQSYRLVT VATLVDRLKI NGALARKCLK DLEEKGLIRQ VVGHSKMKI YTRAVGAAAE

UniProtKB: 40S ribosomal protein S25

+
Macromolecule #20: Ribosomal protein s27-like protein

MacromoleculeName: Ribosomal protein s27-like protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 8.923572 KDa
SequenceString:
MVLAVDLLNP SPASEARKHK LKTLVPAPRS FFMDVKCPGC FTITTVFSHA QTVVICQGCT NVLCQPTGGK ARLTEGCSFR RK

UniProtKB: Ribosomal protein s27-like protein

+
Macromolecule #21: 40S ribosomal protein S28-like protein

MacromoleculeName: 40S ribosomal protein S28-like protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 7.74198 KDa
SequenceString:
MDSSKAPVKF VKVTRVLGRT GSRGGVTQVR VEFMDDTTRS IIRNVKGPVR ENDILVLLES EREARRLR

UniProtKB: 40S ribosomal protein S28-like protein

+
Macromolecule #22: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 6.917273 KDa
SequenceString:
MGKVHGSLAR AGKVKSQTPK VEKQEKPKTP KGRAKKRLLY TRRFVNITVV GGKRKMNPNP GN

+
Macromolecule #23: 40S ribosomal protein S27a-like protein

MacromoleculeName: 40S ribosomal protein S27a-like protein / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 17.73176 KDa
SequenceString:
MQIFVKTLTG KTITLEVEPS DTIDTVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKVYTTPK KIKHKRKKVK LAVLKYYKVD SDGKIERLRR ECPNESCGAG VFMASMQDRQ YCGRCHLTYV FDKSS

UniProtKB: 40S ribosomal protein S27a-like protein

+
Macromolecule #25: Trm112

MacromoleculeName: Trm112 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 14.186489 KDa
SequenceString:
MKVLTLNFLT CAVKACKTKP ESFPLHPKDA ELVSDDVEIN PQLLINLLPR LDWNALRITA TELGFPQLPE QPPTAEDLQS DEKVLKDLH TLLMETQIME GKLVCAACGH EYAIREGIAN FLLPSHLV

+
Macromolecule #26: Methyltransferase-like protein

MacromoleculeName: Methyltransferase-like protein / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 30.362834 KDa
SequenceString: MSRPEDTLAA DVHYNDAEAR KYTTSSRIQN IQAAMTRRAL ELLNLKSPSL ILDVGCGSGL SGEILSSVPP EEGGPHVWVG MDISASMLD VALQRDVEGD LLLSDIGQGV PFRPGTFDAA ISISAIQWLC NADTSETSPA GRLTRFFNGL YAALKRGGMA V CQFYPKND ...String:
MSRPEDTLAA DVHYNDAEAR KYTTSSRIQN IQAAMTRRAL ELLNLKSPSL ILDVGCGSGL SGEILSSVPP EEGGPHVWVG MDISASMLD VALQRDVEGD LLLSDIGQGV PFRPGTFDAA ISISAIQWLC NADTSETSPA GRLTRFFNGL YAALKRGGMA V CQFYPKND VQKQMITSAA IKAGFGAGLL EDDPGTKNVK VYLVLTVGST AVQAKGGDIT GVVEGMDNVE VLDNRKKAKP KS GKGEIKK GSKAWIIKKK EQMERKGKIV KRTSKYTGRS RGPKF

UniProtKB: Methyltransferase-like protein

+
Macromolecule #27: Bms1-type G domain-containing protein

MacromoleculeName: Bms1-type G domain-containing protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 93.76693 KDa
SequenceString: MPGPVSNGQH HHRPTTKTKH KPFKSRHATK RALRDAAKGR VPNEKGQRKT PHQQVMSKLD RRNQAKQRLL EKSREHAKEV SVFNGRDGA PRNVAVIPLC PDTDAVAAIK ALNDSIDIEA DVRDGVFRVP VDRFKQKLQY IALNRDLTAC LDACSVADFI V LVLSAHVE ...String:
MPGPVSNGQH HHRPTTKTKH KPFKSRHATK RALRDAAKGR VPNEKGQRKT PHQQVMSKLD RRNQAKQRLL EKSREHAKEV SVFNGRDGA PRNVAVIPLC PDTDAVAAIK ALNDSIDIEA DVRDGVFRVP VDRFKQKLQY IALNRDLTAC LDACSVADFI V LVLSAHVE VDELGELILR SVESQGMSTL FTVVQGLNRI EPQKQRLSVV ASLKSYITHF HPEQEKIYNL DNRQECSNLM RS LCSTTPK GVRWRDERSW MLIEDVKFGA SADEPSIVTG VVRGKGLKAD RLVQIGDWGT FQVEKITAAP LTVRKRKGDD TAM EDVEQI LDTPTEDRDG LEDLAPEEAV MRDDDEDEMM DAASTVTTSK KGVLLDDHRY YSEDEEEEEE RKLPKRVPKG TSKY QAAWF LDDDDISDSG TDLEDLDMED AQSEASEESA RPEDGIEGLA GADDAMTEAG PSEYPQSVAF EEPDEEEDMR ALEAY RKLK RTEAEDDREF PDEIELHPKV LARERLAKYR GLKSMRTSPW VEEEDRAFEP EEWQRLLRIP DYRASRIRAA REALVG GVA PGTRVNVYVK GLPGRARESY KPGQPLPLVS LLRHEHKRTA MNILINLPSD FPASIKSKSE MIVQYGARRF VINPLFS QP GSTPNDVHKY CRYLHPGQSA VATFMGPVTW GSVPALFFMR DPPTSNDPEA EEQTSASTLP LTLVATGTTL PPSTKRVI A KRAILTGHPY HIHKKIVTVR YMFFNREDVE WFKALPLWTK RGRSGYIKEP LGTHGYFKAT FDGRINPQDT IGVSLYKRV WPRNARPLEG ALLDEKEVAA KKGEDTEMVE

UniProtKB: Bms1-type G domain-containing protein

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Macromolecule #28: Pre-rRNA-processing protein PNO1

MacromoleculeName: Pre-rRNA-processing protein PNO1 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 28.715047 KDa
SequenceString: MPAPTALKQP PPAPEQQAAP AITNENEDEL LIDIQQAAAT LTDPNAAEPP EETMENEMAV DEEGRPRFAP GKNIDPIRRI ETRKIPIPP HRMSALKANW TKYPPLVDHC KLQVRMNIKE KRVELRSSKY TVSNEALQMG ADFVSAFAMG FDIDDAIALL R LDSLYIQS ...String:
MPAPTALKQP PPAPEQQAAP AITNENEDEL LIDIQQAAAT LTDPNAAEPP EETMENEMAV DEEGRPRFAP GKNIDPIRRI ETRKIPIPP HRMSALKANW TKYPPLVDHC KLQVRMNIKE KRVELRSSKY TVSNEALQMG ADFVSAFAMG FDIDDAIALL R LDSLYIQS FDIKDVRQTL GPDALSRAIG RIAGKDGKTK FAIENATKTR IVLAGSKVHI LGAFENIGMA RESIVSLVLG AQ PGKVYNN LRIIASRMKE RW

UniProtKB: Pre-rRNA-processing protein PNO1

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Macromolecule #29: Bystin-like protein

MacromoleculeName: Bystin-like protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 54.319605 KDa
SequenceString: MPKAIAASSH KRRHNPLEDD ILATGILKNR EGRPSKRANK KVAEEENYVD SKASRKILAM SRELMDEEEQ QLKNKQVTVA STAFDFDPS RMDHDEDDQE EFVNNEEWGD EDEDAGDNDN EVDAADLEIF NRFVQPTMKD DPLLTHGWDQ KPADGEEEKE E QTNLADLI ...String:
MPKAIAASSH KRRHNPLEDD ILATGILKNR EGRPSKRANK KVAEEENYVD SKASRKILAM SRELMDEEEQ QLKNKQVTVA STAFDFDPS RMDHDEDDQE EFVNNEEWGD EDEDAGDNDN EVDAADLEIF NRFVQPTMKD DPLLTHGWDQ KPADGEEEKE E QTNLADLI LQKIAEKEAM TGGQNGGNPI EEDYEIPPKV VEVFTKIGLI LARYKSGPLP KPFKVLPTIP HWEDIIQLTR PD LWTPNAC YAATRIFVSA KPQVVQRFME MIILERVRED IHENKKLNVH LFNCLKKALY KPAGFFKGFL FPLAASGTCT LRE AQIISA VLARISIPVL HSAAAIKTLC DIAAEQASQR AECVSATNYL LKVLLEKRYA LPWQCIDALV FHFLRYASMA REGD GAPKA LPVIFHQCLL VFAQRYRNDI TEDQREALLD LLLTHGHEKI APEIRKELLA GRGRGIPVQQ PQPTFDGDDT MLVDS

UniProtKB: Bystin-like protein

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Macromolecule #30: Putative low-temperature viability protein

MacromoleculeName: Putative low-temperature viability protein / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 49.784793 KDa
SequenceString: MAKGKWIDKK TATHFQLVYR PQNDPLIHDE NAPQMVLNPT QPPNSKGKGK GKTLSELASE LGDDVRSVRA NEGEAANYGI FFDDTEYDY MQHLREIGTG EGEAVFIEAN PVINRGKKGG KQKQSLEEAL RQLDLKNKSE ELLDPEILPN KNLQHVSYQA Q QDVPDEIA ...String:
MAKGKWIDKK TATHFQLVYR PQNDPLIHDE NAPQMVLNPT QPPNSKGKGK GKTLSELASE LGDDVRSVRA NEGEAANYGI FFDDTEYDY MQHLREIGTG EGEAVFIEAN PVINRGKKGG KQKQSLEEAL RQLDLKNKSE ELLDPEILPN KNLQHVSYQA Q QDVPDEIA GFQPDMDPRL REVLQALEDD AYVDEEGGEE IFQELAKDAR ELSQDEFEET FDDFEEDEGW ESDRTVKAEK KP EPAQTGD GDAQPASDTK PGQQTSGDTA EGEVPPEATS DAWMEEFKKF KSDQKAAERK SAKARAAPSE IQSSIWTVTT NGG RRKHRK GALTNPSTYS MTSSSLLRTE QLSILDARFE KLEEEYNAEY DEMASMSAVS TASTVQGPTR QDFDGLLDEF LDNY QMAGK KRVKKGKLSG VEQFDEIRRE LGPPRIPAKY LGKGKGKARV

UniProtKB: Low-temperature viability protein

+
Macromolecule #31: Ribosomal RNA-processing protein 12-like conserved domain-contain...

MacromoleculeName: Ribosomal RNA-processing protein 12-like conserved domain-containing protein
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 136.940438 KDa
SequenceString: MASLKEKLDK IRSPNLENQK QTAAVLEAVE AAFKERNTEP TPTAYFAALL SLLNNDNGKN ISHPVVYLLD VVTPFAPAPL LRAKFTQIL TLLAPVLSQP EADAPLIRSS IGCLEGLLLA QDAAGWEMSA AQIGPRRVVG GLLSFALDPR PKVRKRAQDA L RKILRNPP ...String:
MASLKEKLDK IRSPNLENQK QTAAVLEAVE AAFKERNTEP TPTAYFAALL SLLNNDNGKN ISHPVVYLLD VVTPFAPAPL LRAKFTQIL TLLAPVLSQP EADAPLIRSS IGCLEGLLLA QDAAGWEMSA AQIGPRRVVG GLLSFALDPR PKVRKRAQDA L RKILRNPP PSPSLDHPVA PMCAETAMMS LRAVFDKATK SRKEKKGSES AHDPELIHAL QLVKTIASGS GGWPSKKIEP LC ELLLGIA RHGNEHMSMA VFEIFEMIFE GMADEVASAK LPRLLEIIKE LRPAPNDVQL LPPWIAILSR AYDVAAQISP EET FQEILE PFNLVASYLE SEAKNIRISA SECLVSFLAN CVPPQVLVEP SIYDEKVIEK LVQTVEGLLT IKYQAAWLET FNVI GAMFD ALRWRSHPYL LGITKTIGEI RTNDSFTGKQ EADVVLGKAI RAMGPEAVLN VLPLNLIKPA RDQPGRAWML PLLRD YTTN TNLNHFKTEL VPLSAAMYQR VLEHGNAPKT MEVKIFETVV QQIWSILPGY CDLPLDLTEA FDTAFAEMLT TVLYEQ PEL RLDVCRALRA LVESNQAIVS AGDVEDPVIL CRVSKQTAQK NLEYLGERFA ADILAVLFNV YSTTTPQKRG PVLQTIN SF LSIIPKTRLM ETFDLVCEKL AQALQEPVQK PKNPNQEQVP STAHTLMDLV VTMSIYLPRE SFEALFKIAA LVVFKDDD P QIQKKAYKLI PRLAESEVGR AALEQRHEEV QQLLLSAADK VSSPARRERL AAIAALIPII PNTSLHFIPS ILSEVVIGC KEHNEKARTT AFDLLVLMGH KMVEANGSLI DNSKVPHMPK DAPKATASLE EYMTMVSAGL AGSTPHMISA TILAITRVLY EFREALNKE TLTDLVQTMD LFLTSNNREI VKSVLGFVKV CIISLPTDLM LPRLPTLIPN VLVWSHEHKG HFRAKVKHIF E RMIRRFGV ETVQKHTPEA DRKLITNIRK TKERAKRKKE AARNGAGDGS DAEVAGKRKS RFDNEYDQAL NSSDSESEAS DS DDSDIEM TGRRQKKKRE TGGTYILEDQ DEPLDLLDRN ALANISTTKP IKPKKKDDKK SKPKINADGK LIIGEDSDIE EKN DDGAMD LDNEESGVNA YVQALKTGAR RGQRGKLKWD RHRKGQNDDD SDESDNDMEV DEGAAAKALR DSRKGKKAGK AAKP VKGGI AAKVGRRGLG VEKKRGPSVL FGGRINKKGR

UniProtKB: Uncharacterized protein

+
Macromolecule #24: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 24 / Number of copies: 1
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 578.795562 KDa
SequenceString: UACCUGGUUG AUUCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUAUACAG CGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUCGUUUAU UUGAUAGUAC CUUACUACAU GGAUAACCGU GGUAAUUCUA G AGCUAAUA ...String:
UACCUGGUUG AUUCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUAUACAG CGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUCGUUUAU UUGAUAGUAC CUUACUACAU GGAUAACCGU GGUAAUUCUA G AGCUAAUA CAUGCUGAAA AUCCCGACUU CGGAAGGGAU GUAUUUAUUA GAUUAAAAAC CAAUGCCCUU CGGGGCUUUC UG GUGAUUC AUAAUAACUU CUCGAAUCGC ACGGCCUUGC GCCGGCGAUG GUUCAUUCAA AUUUCUGCCC UAUCAACUUU CGA CGGCUG GGUAUUGGCC AGCCGUGGUG ACAACGGGUA ACGGAGGGUU AGGGCUCGAC CCCGGAGAAG GAGCCUGAGA AACG GCUAC UACAUCCAAG GAAGGCAGCA GGCGCGCAAA UUACCCAAUC CCGACACGGG GAGGUAGUGA CAAUAAAUAC UGAUA CAGG GCUCUUUCGG GUCUUGUAAU UGGAAUGAGU ACAAUUUAAA UCCCUUAACG AGGAACAAUU GGAGGGCAAG UCUGGU GCC AGCAGCCGCG GUAAUUCCAG CUCCAAUAGC GUAUAUUAAA GUUGUUGAGG UUAAAAAGCU CGUAGUUGAA CCUUGGG CC UAGCCCGCCG GUCCGCCUCA CCGCGUGCAC UGGCUGGGCU GGGCCUUUCC UUCUGGAGAA CCGCAUGCCC UUCACUGG G UGUGUCGGGG AACCAGGACU UUUACUCUGA ACAAAUUAGA UCGCUUAAAG AAGGCCUAUG CUUGAAUACA UUAGCAUGG AAUAAUAGAA UAGGACGUGU GGUUCUAUUU UGUUGGUUUC UAGGACCGCC GUAAUGAUUA AUAGGGACAG UCGGGGGCGU CAGUAUUCA AUUGUCAGAG GUGAAAUUCU UGGAUUUAUU GAAGACUAAC AACUGCGAAA GCAUUCGCCA AGGAUGUUUU C AUUAAUCA GGAACGAAAG UUAGGGGAUC GAAGACGAUC AGAUACCGUC GUAGUCUUAA CCAUAAACUA UGCCGAUUAG GG AUCGGAC GGCGUUUAUU UUUGACCCGU UCGGCACCUU ACGAUAAAUC AAAAUGUUUG GGCUCCUGGG GGAGUAUGGU CGC AAGGCU GAAACUUAAA GAAAUUGACG GAAGGGCACC ACCAGGGGUG GAGCCUGCGG CUUAAUUUGA CUCAACACGG GGAA ACUCA CCAGGUCCAG ACACGAUGAG GAUUGACAGA UUGAGAGCUC UUUCUUGAUU UCGUGGGUGG UGGUGCAUGG CCGUU CUUA GUUGGUGGAG UGAUUUGUCU GCUUAAUUGC GAUAACGAAC GAGACCUUAA CCUGCUAAAU AGCCCGUAUU GCCUUG GCA GUACGCCGGC UUCUUAGAGG GACUAUCGGC UCAAGCCGAU GGAAGUUUGA GGCAAUAACA GGUCUGUGAU GCCCUUA GA UGUUCUGGGC CGCACGCGCG CUACACUGAC AGAGCCAGCG AGUACUCCCU UGGCCGAGAG GCCCGGGUAA UCUUGUUA A ACUCUGUCGU GCUGGGGAUA GAGCAUUGCA AUUAUUGCUC UUCAACGAGG AAUCCCUAGU AAGCGCAAGU CAUCAGCUU GCGUUGAUUA CGUCCCUGCC CUUUGUACAC ACCGCCCGUC GCUACUACCG AUUGAACGGC UCAGUGAGGC UUUCGGACUG GCCCAGAGA GGUCGGCAAC GACCACUCAG GGCCGGAAAG UUAUCCAAAC UCGGUCGUUU AGAGGAAGUA AAAGUCGUAA C AAGGUCUC CGUAGGUGAA CCUGCGGAGG GAUCAUUA

+
Macromolecule #32: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 32 / Number of copies: 2 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #33: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 33 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #34: water

MacromoleculeName: water / type: ligand / ID: 34 / Number of copies: 4 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correctionSoftware - Name: RELION / Details: Relion / Type: NONE
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6g18

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 52758
Initial angle assignmentType: OTHER / Software - Name: RELION / Details: Relion
Final angle assignmentType: OTHER / Software - Name: RELION / Details: Relion
FSC plot (resolution estimation)

-
Atomic model buiding 1

Initial modelPDB ID:

6rxu


Chain - Source name: PDB / Chain - Initial model type: experimental model
Output model

PDB-9xac:
Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Rrp12-A1

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