Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Nucleoplasmic checkpoint of the 40S ribosomal decoding center maturation.
Journal, issue, pages	Cell Rep, Vol. 45, Issue 6, Page 117545, Year 2026
Publish date	Jun 11, 2026
Authors	Benjamin Lau / Yi Li / Jingyi Zhu / Xianwen Ye / Paulina Fischer / Xiaying Hong / Rui Yuan / Roland Beckmann / Ed Hurt / Jingdong Cheng /
PubMed Abstract	The decoding center (DC) is a key ribosomal structure for accurate translation, assembled in a multi-step process that starts on nucleolar pre-ribosomes and ends in the cytoplasm. While late ...The decoding center (DC) is a key ribosomal structure for accurate translation, assembled in a multi-step process that starts on nucleolar pre-ribosomes and ends in the cytoplasm. While late cytoplasmic steps and their checkpoint mechanisms are well characterized, the regulation of early nucleoplasmic DC assembly is unclear. Here, we show that the essential assembly factor Rrp12 plays a central coordinating role. Using Chaetomium thermophilum and cryo-electron microscopy analyses of fifteen pre-40S intermediates, we demonstrate that Rrp12 C terminus truncation: (1) inhibits release of the Utp14-Dhr1 pair, (2) displaces Tsr1, (3) promotes premature stabilization of h28, and (4) prevents h44 formation. These defects impair final 18S rRNA processing and prematurely activate the quality control kinase Rio1. Our results reveal a nucleoplasmic checkpoint during DC formation and establish Rrp12 as a critical regulator ensuring accurate assembly and orderly ribosome maturation.
External links	Cell Rep / PubMed:42275223
Methods	EM (single particle)
Resolution	2.8 - 4.9 Å
Structure data	66677 9xa7 EMDB-66677, PDB-9xa7: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state A Method: EM (single particle) / Resolution: 3.2 Å 66678 9xa8 EMDB-66678, PDB-9xa8: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state B1 Method: EM (single particle) / Resolution: 2.9 Å 66679 9xa9 EMDB-66679, PDB-9xa9: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Tsr1-1 Method: EM (single particle) / Resolution: 3.3 Å 66680 9xaa EMDB-66680, PDB-9xaa: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Tsr1-2 Method: EM (single particle) / Resolution: 3.1 Å 66681 9xab EMDB-66681, PDB-9xab: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Tsr1-3 Method: EM (single particle) / Resolution: 3.2 Å 66682 9xac EMDB-66682, PDB-9xac: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Rrp12-A1 Method: EM (single particle) / Resolution: 3.3 Å 66683 9xad EMDB-66683, PDB-9xad: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Rrp12-A2 Method: EM (single particle) / Resolution: 3.0 Å 66684 9xae EMDB-66684, PDB-9xae: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 WT) from Chaetomium thermophilum, state Rio2-C Method: EM (single particle) / Resolution: 3.0 Å 66685 9xaf EMDB-66685, PDB-9xaf: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state a Method: EM (single particle) / Resolution: 3.0 Å 66686 9xag EMDB-66686, PDB-9xag: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state B1 Method: EM (single particle) / Resolution: 2.8 Å 66687 9xah EMDB-66687, PDB-9xah: Cryo-EM structure of the 90S pre-ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state B1* Method: EM (single particle) / Resolution: 3.1 Å 66688 9xai EMDB-66688, PDB-9xai: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state Tsr1-1* Method: EM (single particle) / Resolution: 4.1 Å 66689 9xaj EMDB-66689, PDB-9xaj: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state Rrp12-A1* Method: EM (single particle) / Resolution: 3.1 Å 66690 9xak EMDB-66690, PDB-9xak: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 Mut) from Chaetomium thermophilum, state Rrp12-A1 Method: EM (single particle) / Resolution: 3.3 Å EMDB-66691*: Cryo-EM structure of the pre-40S ribosome (Enp1-Rrp12 Mut ) from Chaetomium thermophilum, state Rio2-C Method: EM (single particle) / Resolution: 4.9 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-HOH: WATER
Source	thermochaetoides thermophila (fungus)
Keywords	RIBOSOME / 90S pre-ribosome / Rrp12 / Enp1 / ribosome biogenesis / 90S / pre-40S ribosome