- EMDB-5395: 9-fold symmetric rATcpn-beta in apo state -
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基本情報
登録情報
データベース: EMDB / ID: EMD-5395
タイトル
9-fold symmetric rATcpn-beta in apo state
マップデータ
Reconstruction of the 9-fold symmetric class of rATcpn-beta in apo state
試料
試料: rATcpn-beta in apo state
タンパク質・ペプチド: Group II chaperonin beta
キーワード
Group II chaperonin / thermosome
機能・相同性
機能・相同性情報
chaperonin-containing T-complex / ATP-dependent protein folding chaperone / unfolded protein binding / ATP hydrolysis activity / ATP binding / identical protein binding 類似検索 - 分子機能
ジャーナル: Protein Cell / 年: 2013 タイトル: Flexible interwoven termini determine the thermal stability of thermosomes. 著者: Kai Zhang / Li Wang / Yanxin Liu / Kwok-Yan Chan / Xiaoyun Pang / Klaus Schulten / Zhiyang Dong / Fei Sun / 要旨: Group II chaperonins, which assemble as double-ring complexes, assist in the refolding of nascent peptides or denatured proteins in an ATP-dependent manner. The molecular mechanism of group II ...Group II chaperonins, which assemble as double-ring complexes, assist in the refolding of nascent peptides or denatured proteins in an ATP-dependent manner. The molecular mechanism of group II chaperonin assembly and thermal stability is yet to be elucidated. Here, we selected the group II chaperonins (cpn-α and cpn-β), also called thermosomes, from Acidianus tengchongensis and investigated their assembly and thermal stability. We found that the binding of ATP or its analogs contributed to the successful assembly of thermosomes and enhanced their thermal stabilities. Cpn-β is more thermally stable than cpn-α, while the thermal stability of the hetero thermosome cpn-αβ is intermediate. Cryo-electron microscopy reconstructions of cpn-α and cpn-β revealed the interwoven densities of their non-conserved flexible N/C-termini around the equatorial planes. The deletion or swapping of their termini and pH-dependent thermal stability assays revealed the key role of the termini electrostatic interactions in the assembly and thermal stability of the thermosomes.