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- EMDB-49872: Ciliary tip central pair -

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Basic information

Entry
Database: EMDB / ID: EMD-49872
TitleCiliary tip central pair
Map data
Sample
  • Organelle or cellular component: Ciliary tip central pair
    • Protein or peptide: x 10 types
  • Ligand: x 3 types
Keywordscilia / microtubules / central pair / PROTEIN BINDING
Function / homology
Function and homology information


actomyosin structure organization / motile cilium / regulation of cytoskeleton organization / axoneme / microtubule-based process / structural constituent of cytoskeleton / microtubule binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / cytoskeleton ...actomyosin structure organization / motile cilium / regulation of cytoskeleton organization / axoneme / microtubule-based process / structural constituent of cytoskeleton / microtubule binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / cytoskeleton / hydrolase activity / GTPase activity / GTP binding / metal ion binding / nucleus / cytoplasm
Similarity search - Function
LSDAT, prokaryote / SLOG in TRPM, prokaryote / : / CH-like domain in sperm protein / CH-like domain in sperm protein / : / Dpy-30 motif / Dpy-30 motif / Calponin homology domain / CH domain superfamily ...LSDAT, prokaryote / SLOG in TRPM, prokaryote / : / CH-like domain in sperm protein / CH-like domain in sperm protein / : / Dpy-30 motif / Dpy-30 motif / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / C2 domain / C2 domain / C2 domain profile. / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / C2 domain superfamily / Armadillo-type fold
Similarity search - Domain/homology
Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Tubulin alpha chain / Tubulin beta chain / Sperm flagellar protein / Dpy-30 motif protein / C2 domain protein / Cation channel family protein
Similarity search - Component
Biological speciesTetrahymena thermophila CU428 (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsLegal TL / Bui KH
Funding support Canada, 2 items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR)PJT-156354 Canada
Natural Sciences and Engineering Research Council (NSERC, Canada)RGPIN-2022-04774 Canada
CitationJournal: Curr Biol / Year: 2025
Title: Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1.
Authors: Thibault Legal / Ewa Joachimiak / Mireya Parra / Wang Peng / Amanda Tam / Corbin Black / Mayukh Guha / Chau Anh Nguyen / Avrin Ghanaeian / Melissa Valente-Paterno / Gary Brouhard / Jacek ...Authors: Thibault Legal / Ewa Joachimiak / Mireya Parra / Wang Peng / Amanda Tam / Corbin Black / Mayukh Guha / Chau Anh Nguyen / Avrin Ghanaeian / Melissa Valente-Paterno / Gary Brouhard / Jacek Gaertig / Dorota Wloga / Khanh Huy Bui /
Abstract: Motile cilia are unique organelles with the ability to move autonomously. The force generated by beating cilia propels cells and moves fluids. The ciliary skeleton is made of peripheral doublet ...Motile cilia are unique organelles with the ability to move autonomously. The force generated by beating cilia propels cells and moves fluids. The ciliary skeleton is made of peripheral doublet microtubules and a central pair (CP) with a distinct structure at the tip. In this study, we present a high-resolution structure of the CP in the ciliary tip of the ciliate Tetrahymena thermophila and identify several tip proteins that bind and form unique patterns on both microtubules of the tip CP. Two of those proteins that contain tubulin polymerization-promoting protein (TPPP)-like domains, TLP1 and TLP2, bind to high curvature regions of the microtubule. TLP2, which contains two TPPP-like domains, is an unusually long protein that wraps laterally around half a microtubule and forms the bridge between the two microtubules. Moreover, we found that the conserved protein SPEF1 binds to both microtubule seams and crosslinked the two microtubules. In vitro, human SPEF1 binds to the microtubule seam as visualized by cryoelectron tomography and subtomogram averaging. Single-molecule microtubule dynamics assays indicate that SPEF1 stabilizes microtubules in vitro. Together, these data show that the proteins in the tip CP maintain stable microtubule structures and play important roles in maintaining the integrity of the axoneme.
History
DepositionMar 21, 2025-
Header (metadata) releaseJul 30, 2025-
Map releaseJul 30, 2025-
UpdateJul 30, 2025-
Current statusJul 30, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_49872.png

Downloads & links

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Map

FileDownload / File: emd_49872.map.gz / Format: CCP4 / Size: 1.2 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.37 Å/pix.
x 680 pix.
= 931.6 Å		1.37 Å/pix.
x 680 pix.
= 931.6 Å		1.37 Å/pix.
x 680 pix.
= 931.6 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.37 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.17
Minimum - Maximum-0.0067419284 - 2.7976015
Average (Standard dev.)0.005696163 (±0.056240927)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions680680680
Spacing680680680
CellA=B=C: 931.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_49872_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_49872_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Ciliary tip central pair

EntireName: Ciliary tip central pair
Components
  • Organelle or cellular component: Ciliary tip central pair
    • Protein or peptide: Tubulin alpha chain
    • Protein or peptide: Tubulin beta chain
    • Protein or peptide: Sperm flagellar protein
    • Protein or peptide: TLP1
    • Protein or peptide: C2 domain protein
    • Protein or peptide: CFAP213
    • Protein or peptide: TLP2
    • Protein or peptide: Cation channel family protein
    • Protein or peptide: Dpy-30 motif protein
    • Protein or peptide: Unknown
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: GUANOSINE-5'-DIPHOSPHATE

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Supramolecule #1: Ciliary tip central pair

SupramoleculeName: Ciliary tip central pair / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#10
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)

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Macromolecule #1: Tubulin alpha chain

MacromoleculeName: Tubulin alpha chain / type: protein_or_peptide / ID: 1 / Number of copies: 39 / Enantiomer: LEVO
EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 49.639035 KDa
SequenceString: MREVISIHVG QGGIQVGNAC WELFCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRAVFLDL EPTVIDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLCL DRIRKLADNC TGLQGFLVFN SVGGGTGSGL GSLLLERLSV D YGKKSKLG ...String:
MREVISIHVG QGGIQVGNAC WELFCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRAVFLDL EPTVIDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLCL DRIRKLADNC TGLQGFLVFN SVGGGTGSGL GSLLLERLSV D YGKKSKLG FTIYPSPQVS TAVVEPYNSI LSTHSLLEHT DVAVMLDNEA IYDICRRNLD IERPTYTNLN RLIAQVISSL TA SLRFDGA LNVDITEFQT NLVPYPRIHF MLSSYAPIIS AEKAYHEQLS VAEITNSAFE PANMMAKCDP RHGKYMACSM MYR GDVVPK DVNASIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVMRAVC MISNSTAIAE VFSRLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GIETAEGEGE EEGY

UniProtKB: Tubulin alpha chain

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Macromolecule #2: Tubulin beta chain

MacromoleculeName: Tubulin beta chain / type: protein_or_peptide / ID: 2 / Number of copies: 39 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 49.617676 KDa
SequenceString: MREIVHIQGG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGRYV PRAILMDLEP GTMDSVRAGP FGQLFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAEGCDC LQGFQITHSL GGGTGSGMGT LLISKVREEY P DRIMETFS ...String:
MREIVHIQGG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGRYV PRAILMDLEP GTMDSVRAGP FGQLFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAEGCDC LQGFQITHSL GGGTGSGMGT LLISKVREEY P DRIMETFS VVPSPKVSDT VVEPYNATLS VHQLVENADE CMVIDNEALY DICFRTLKLT TPTYGDLNHL VSAAMSGVTC CL RFPGQLN SDLRKLAVNL IPFPRLHFFM IGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMCAADPRH GRYLTASALF RGR MSTKEV DEQMLNVQNK NSSYFVEWIP NNIKSSICDI PPKGLKMAVT FVGNSTAIQE MFKRVAEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FEEEEGEN

UniProtKB: Tubulin beta chain

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Macromolecule #3: Sperm flagellar protein

MacromoleculeName: Sperm flagellar protein / type: protein_or_peptide / ID: 3 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 24.702463 KDa
SequenceString: MDAPPLNEEE LNQIYNWIDG IPLSRPKKNI TRDFADGCML AEVISHYCPK LVEVHNYSQA HSVTQKLYNW NTLNQRVLKK MGFQISKND IDCIINAVPD AVERVLKVCQ IKLDKYLDDQ KKKKDQQQYI ENAQMMQNGN KLQLGNANNP YQYAEFLNPK E VSQMLFQR ...String:
MDAPPLNEEE LNQIYNWIDG IPLSRPKKNI TRDFADGCML AEVISHYCPK LVEVHNYSQA HSVTQKLYNW NTLNQRVLKK MGFQISKND IDCIINAVPD AVERVLKVCQ IKLDKYLDDQ KKKKDQQQYI ENAQMMQNGN KLQLGNANNP YQYAEFLNPK E VSQMLFQR DTTINELRAT IEILELKIKK MDQLVKLKDS KIQSLINKLS QAGLS

UniProtKB: Sperm flagellar protein

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Macromolecule #4: TLP1

MacromoleculeName: TLP1 / type: protein_or_peptide / ID: 4 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 88.103156 KDa
SequenceString: MSEENNENQE NQYNDDDQYN HNEENEDNEQ QEQVDYANHD NDENQEHDPA NEEEQGEQNE NVNNDNNNNY DEEEDGKRNE LDDYLSGGG SNRNDKQDQQ SSSKRGSNFG VINKPDDNEN YLRNKDILDN FFKQYQGEVQ NYFNEFQQKQ IQDSQENGNN N FYSKQNNQ ...String:
MSEENNENQE NQYNDDDQYN HNEENEDNEQ QEQVDYANHD NDENQEHDPA NEEEQGEQNE NVNNDNNNNY DEEEDGKRNE LDDYLSGGG SNRNDKQDQQ SSSKRGSNFG VINKPDDNEN YLRNKDILDN FFKQYQGEVQ NYFNEFQQKQ IQDSQENGNN N FYSKQNNQ QSQKEVSIVA SDVPLVLRIS FYYDNITFHP TKNDQEELFN KPNSYKSIMD QLQKFFAVRR NIRNFDPNET KV TYSDFVE FSYFFIENND DLFLTQQGKE ELWIQQQQES QLEKELRENI DKYENMLKKT TNPQEKLIIQ NTIENLKQQL TLL LNKDQG KKGKNLTKEQ IRMKNLKEAF NFYCKQQNVT GVAFTFDRIV HEQNVINLAC FMLFLKQFNI LNKNKYVTKR EIQI MFKKY ALNYKELDLD HFKIMIEKVA IAFYKEEENL SNIDRVEKLY QYIEIDNIHK FRSKFGLLNQ PFNIQVKDGF RLLPF DQGR DIVLKKVDPV VRQKVKQFQE FKKSQQALQI MQLDQKNINQ SKLAPRSMEN NRRSTPNLQQ SSVYSQPGGI GSYANK KIK NQIGKLDAGG KVSWQQLEQL SFSDMKNLNN GQFRPTDLFN ENDDEEDKIY LAEYNLQEDR QRKIQEQMVN KKGQENY GQ IKEPVRPSLR YLQKHDPNYG KNGYSPSVGN LHRVHASAPE YYSPQNHMNQ KQLAINQSYI QRAQQVDQDQ RMKEQAMM Q KIMNNHDLKV QRGINAMNKR K

UniProtKB: Uncharacterized protein

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Macromolecule #5: C2 domain protein

MacromoleculeName: C2 domain protein / type: protein_or_peptide / ID: 5 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 57.465531 KDa
SequenceString: MPQKVSITLK NIEVKRKEQK FKSMHPWAVV MFDGQKKTTQ PVQNGDLQPE FNQTWVFQRN SDVNITVSIY KKEYIYDALK NEKEIDLLI GKGKLNILSV LKKAIQQPQE IMVDLVYDFD NKLAAQAYLT IHIQQGADLE EYRNKEQIKQ IKSKYESCLT V TNLQLSLD ...String:
MPQKVSITLK NIEVKRKEQK FKSMHPWAVV MFDGQKKTTQ PVQNGDLQPE FNQTWVFQRN SDVNITVSIY KKEYIYDALK NEKEIDLLI GKGKLNILSV LKKAIQQPQE IMVDLVYDFD NKLAAQAYLT IHIQQGADLE EYRNKEQIKQ IKSKYESCLT V TNLQLSLD SFKPEAYYYL KIRNSNGSHK TTPQNGGASM MFQGDFDIYF RGQKIVYIDV QEVLKDEILD FITADDLVGI GT LDFEKVD INQSQQRAVI RHKGKQIGSV QCQASYEIFK QEQPEEHASM IRQSNDELLE QIKNLEKKDK FWLSPERYQL DLK LKKKQI LDRRQMLMKN RSSAPQLGNE SYLFMKPDER NIVQKPDIVI STTTVKLPKL KTNNNVEVPT QYSKQYFRDI HGVG FSTVE SSSSLQRSVP KFSIPEQKRF SDPKDIFPGP GRYGTSGVAN DLYLPKKFQK ITAISEVRTT KQERKERVQI DTGLK KPID RQQQQTTQNP K

UniProtKB: C2 domain protein

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Macromolecule #6: CFAP213

MacromoleculeName: CFAP213 / type: protein_or_peptide / ID: 6 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 37.99841 KDa
SequenceString: MYQNQYQQPQ QQYGYNQGQQ GQQYSQAYQQ QPYQQQQGSP YQQQQNYPQG YGAQQQAYQQ QQQGYAQQQG YPAQNQQYYQ EDYDSLQQY QDNFNSVQPR TRLEREAMKD KESIEKTRIN QRVGYETRNT DVKQLLHNPD PKSTLFIPEN QRFDKDFSVF D KQQRDQRF ...String:
MYQNQYQQPQ QQYGYNQGQQ GQQYSQAYQQ QPYQQQQGSP YQQQQNYPQG YGAQQQAYQQ QQQGYAQQQG YPAQNQQYYQ EDYDSLQQY QDNFNSVQPR TRLEREAMKD KESIEKTRIN QRVGYETRNT DVKQLLHNPD PKSTLFIPEN QRFDKDFSVF D KQQRDQRF ATKEVALEKH RIEALERESK RWEQMENQVN KEQVKRQFQA EVLKAGKRNT NGMPFNPITL EYEKSSAGDS LK KRDEMAK VRGYVRAENL DTRSNCGYNI LTGEQRIGVE YLVPNHLRDD YKTKVDLKNE FYSIKYKGEQ QNQQNQNKYY

UniProtKB: Uncharacterized protein

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Macromolecule #7: TLP2

MacromoleculeName: TLP2 / type: protein_or_peptide / ID: 7 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 205.367016 KDa
SequenceString: MQIGEDIYNF LQVFNLINPT EGKKISDDKY SIGKNTTNQL ENGQIFAKII RQIAKIQAQS QKRPEQPFPD LDSLKEMNSP AARLYNWNV LQECFRKLQI NLETETKSLI IAGDQDQLSD FIKDIIANVA IYMPELFGKN KGKSKVKQNE YKDEIEVTKV D INKDLTKC ...String:
MQIGEDIYNF LQVFNLINPT EGKKISDDKY SIGKNTTNQL ENGQIFAKII RQIAKIQAQS QKRPEQPFPD LDSLKEMNSP AARLYNWNV LQECFRKLQI NLETETKSLI IAGDQDQLSD FIKDIIANVA IYMPELFGKN KGKSKVKQNE YKDEIEVTKV D INKDLTKC KSCLEFFVVL LSRHLSLPPQ QTASLFTHNN KYLAHLFAKG VKGLFDPIIF FYQEVYQSIP LLLQLFLEDP TK KSMHFSM NCLKPGLVSK SYEVATWAAR LFSKLALEFS ESNLLTVSWD WFVGENGGLN TTLLGLKRHP DMKDLVVQIL LQF ARYNFV ELFTIHMKKA QPDPKDLVGT YLVLLKPLTA TPSACDEILN AGILDEWIDF ALDGSTDEYK NTIDMRTVSL TLLV EVWML FPFKIEDSEY KSNAVLQQLG KACKDKSQSL IVCSLTLLFQ LLIYFGTQKS QFAPNVYRTL TLSIIENHQN VVVRE FIMN NFITVYETLE SIPLNILIEP LVRQIQVTDN VSYFFNVFDF NFFAYISKNE KLQLKNAIQL LDLFFKIYLN NTIFAN VSS VPILNIVNRF IDSETLQEFV IKFVKVALAM FYASEKKKRP KEKVMPLYNN KSAIGQPNLS PGEIEQELIQ AQKRALI VE MIKSIVTINC YELNEKIKPL VAHTNIQIKQ FTKQNNKGMQ SILTLFGNPD TILEKYEKEY IEHQQQLKQE EKERREQE Q NDSLLQDFEG SPKNQGDVLK SIEDNLKKYG IGLKERQTKL TKAEATRLAL LRNPKADPKI LKKLQEIKVN YENREEKKK LEVVKQEQET QKEKEVLRKQ LMRRSLEQGV SNFNQRDAEG VLLFQFGSRE KQIKRENKTG LPMIQYIDLD KEEQRDKDTI NILLRRYNK ILKNVFVKYS NTGFSAQFSN KNVNSFDAIK EHNSLISIPE MYRFVKDYEL SDKLSKEEHQ TLVRIVSQEQ A KKKNKQEV SRDQPEQNKQ IDQKKKLPPA TQSQQNMFLK GSNENKWNFN QNEVKDVKSF DFQGFLDYIV QFAGYIYSKP PE DLRHLPL SASLQKVFDH MREITSKKGQ STALFDDYDN LFFGETEVIK QFNKKLEENP DYPLPQGYKK VKDREIKFEY ALD PKGILP MKESFKYAYL VLNDLFQEQL QFSIIEPKSA TKEVVKAQPL IGITSDVESH YFDMYKSPQV LEQKRVKKPQ MVQS QSVDI LGPWGKPDST KTLPSTLNVK SKYGNQRVLD IDNYRKLDLG LKLAVSTIGY RDRYTAEQCA YCLDDIIKSI ETGDS NQAF RNKKVENKVI KEKKKEEEAQ EEAKKKYEEK RLKDHDFVMS RAKEIIEQNS DKDKQKKEKE LQLKKQKEEE QKKKAD DMS SFWKEKKEVF DQQKQKLIEE QKKIQEEQLR RMQEEKEKKE KEFQEFNKKQ IEKQQEEFKK QKEKEQQEAE KQRNLEE YQ KKLKEKLHMH IVKTDQERIE IEKVSNVKIK ELFQKDDVQS VYKTNEFKLS QLFFYLKKYT YREISRQNIA DNEISYKT F NWFCYRFNIY PEIISNPKDI LLIYRSVTRN KQVIDHKPIG LSEEEFKEAM LRISIKGKKI FNKFSEQLQK GINLNESEM AKIADDENKE SNQEEFQENK SVKSSKTEAL ERMKNVIDYY GNIDEANSHT LEALIYYLGL PNDKLGINEA LKNVMEQGAV PDGKLKEAM KQKIVKDPNE ILRYNPPQNK VKFGRSSQVG HNQSQMANNS QVGSQNGLAE NQENQDQEEN EQEQN

UniProtKB: Uncharacterized protein

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Macromolecule #8: Cation channel family protein

MacromoleculeName: Cation channel family protein / type: protein_or_peptide / ID: 8 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 39.902203 KDa
SequenceString: MSQKTIQSQE ELEENNKNQE EEENVPQQSN KQGSSENLRQ KNRSQENLKQ RKQSPNYYQS SMVQSGVGTS PTRKPNDKTF ITLQKKEEE SQVWKPYDFL FPAGRLAKLV KVFDVYFKNF PFSDLLNKLK LTEGYPIVNL IGAAQSNRGK FYAGLARACF N SEAIIVDS ...String:
MSQKTIQSQE ELEENNKNQE EEENVPQQSN KQGSSENLRQ KNRSQENLKQ RKQSPNYYQS SMVQSGVGTS PTRKPNDKTF ITLQKKEEE SQVWKPYDFL FPAGRLAKLV KVFDVYFKNF PFSDLLNKLK LTEGYPIVNL IGAAQSNRGK FYAGLARACF N SEAIIVDS AIETGLEPYV IRRDLKLVGV APENCVKYPK INSTYKDPYE LSNGHTHLFL VNDKDKTLQW SQETLFKIQL IL KLAEGKV SKKGPRCKIV NVLLADTPNY LDEVRLAVKY DLPIIVCKGS HICDEIIKNS KAQEEEKQWN LVDDEMQELL QKG HFYMLD SLDSEDIAQY IHFFLTFTPY

UniProtKB: Cation channel family protein

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Macromolecule #9: Dpy-30 motif protein

MacromoleculeName: Dpy-30 motif protein / type: protein_or_peptide / ID: 9 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 11.293362 KDa
SequenceString:
MAEKKKQQQS KGSSQGLQNI ADEDDEQNQN QDQDDGLLTG ASNQQGGGGS KFQQLPLRDY YESSVTQVLL EGLRELGAKR PENPIQFLG KFLLDRDPEK KK

UniProtKB: Dpy-30 motif protein

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Macromolecule #10: Unknown

MacromoleculeName: Unknown / type: protein_or_peptide / ID: 10 / Number of copies: 27 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote)
Molecular weightTheoretical: 3.252 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #11: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 11 / Number of copies: 39 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Macromolecule #12: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 12 / Number of copies: 39 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #13: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 13 / Number of copies: 39 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: EMDB MAP
EMDB ID:

40896

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 66174
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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