Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of the ciliary tip central pair reveals the unique role of the microtubule-seam binding protein SPEF1.
Journal, issue, pages	Curr Biol, Vol. 35, Issue 14, Page 3404-33417.e6, Year 2025
Publish date	Jul 21, 2025
Authors	Thibault Legal / Ewa Joachimiak / Mireya Parra / Wang Peng / Amanda Tam / Corbin Black / Mayukh Guha / Chau Anh Nguyen / Avrin Ghanaeian / Melissa Valente-Paterno / Gary Brouhard / Jacek Gaertig / Dorota Wloga / Khanh Huy Bui /
PubMed Abstract	Motile cilia are unique organelles with the ability to move autonomously. The force generated by beating cilia propels cells and moves fluids. The ciliary skeleton is made of peripheral doublet ...Motile cilia are unique organelles with the ability to move autonomously. The force generated by beating cilia propels cells and moves fluids. The ciliary skeleton is made of peripheral doublet microtubules and a central pair (CP) with a distinct structure at the tip. In this study, we present a high-resolution structure of the CP in the ciliary tip of the ciliate Tetrahymena thermophila and identify several tip proteins that bind and form unique patterns on both microtubules of the tip CP. Two of those proteins that contain tubulin polymerization-promoting protein (TPPP)-like domains, TLP1 and TLP2, bind to high curvature regions of the microtubule. TLP2, which contains two TPPP-like domains, is an unusually long protein that wraps laterally around half a microtubule and forms the bridge between the two microtubules. Moreover, we found that the conserved protein SPEF1 binds to both microtubule seams and crosslinked the two microtubules. In vitro, human SPEF1 binds to the microtubule seam as visualized by cryoelectron tomography and subtomogram averaging. Single-molecule microtubule dynamics assays indicate that SPEF1 stabilizes microtubules in vitro. Together, these data show that the proteins in the tip CP maintain stable microtubule structures and play important roles in maintaining the integrity of the axoneme.
External links	Curr Biol / PubMed:40651469 / PubMed Central
Methods	EM (subtomogram averaging) / EM (single particle)
Resolution	3.61 - 25.2 Å
Structure data	49760 9ntm EMDB-49760, PDB-9ntm: SPEF1 bound to 14-pf microtubule Method: EM (subtomogram averaging) / Resolution: 7.1 Å 49872 9nw3 EMDB-49872, PDB-9nw3: Ciliary tip central pair Method: EM (single particle) / Resolution: 3.7 Å EMDB-49903: SPEF1 bound to 13-PF microtubule Method: EM (subtomogram averaging) / Resolution: 8.1 Å 70819 9ot2 EMDB-70819, PDB-9ot2: 13PF microtubule symmetry expansion Method: EM (subtomogram averaging) / Resolution: 5.8 Å EMDB-70821: Tip CP C1 Wild Type Method: EM (subtomogram averaging) / Resolution: 19.48 Å EMDB-70823: Tip CP C2 WT Method: EM (subtomogram averaging) / Resolution: 21.98 Å EMDB-70829: SPEF1 KO Tip CP C1 Method: EM (subtomogram averaging) / Resolution: 25.2 Å EMDB-70830: SPEF1 KO Tip CP C2 Method: EM (subtomogram averaging) / Resolution: 23.8 Å EMDB-71226: Refinement C1 PF2-3-4 Method: EM (single particle) / Resolution: 4.1 Å EMDB-71227: Refinement C1 PF13-1-2 Method: EM (single particle) / Resolution: 3.9 Å EMDB-71228: Refinement C1 PF4-5-6 Method: EM (single particle) / Resolution: 4.36 Å EMDB-71229: Refinement C1 PF6-7-8 Method: EM (single particle) / Resolution: 4.36 Å EMDB-71230: Refinement C1 PF8-9-10 Method: EM (single particle) / Resolution: 4.19 Å EMDB-71231: Refinement C1 PF10-11-12-13 Method: EM (single particle) / Resolution: 4.0 Å EMDB-71232: Refinement C2 PF13-1-2 Method: EM (single particle) / Resolution: 3.61 Å EMDB-71233: Refinement C2 PF2-3-4 Method: EM (single particle) / Resolution: 3.68 Å EMDB-71234: Refinement C2 PF4-5-6 Method: EM (single particle) / Resolution: 3.74 Å EMDB-71235: Refinement C2 PF6-7-8 Method: EM (single particle) / Resolution: 3.73 Å EMDB-71236: Refinement C2 PF8-9-10 Method: EM (single particle) / Resolution: 3.72 Å EMDB-71237: Refinement C2 PF10-11-12-13 Method: EM (single particle) / Resolution: 3.74 Å
Chemicals	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM ChemComp-TA1: TAXOL / medication, chemotherapy*YM
Source	homo sapiens (human) recombinant vesicular stomatitis indiana virus rvsv-g/gfp bos taurus (domestic cattle) tetrahymena thermophila cu428 (eukaryote) Tetrahymena thermophila (eukaryote)
Keywords	PROTEIN BINDING / microtubules / spef1 / seam / cilia / central pair / STRUCTURAL PROTEIN / microtubule