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- EMDB-36973: CryoEM structure of LonC protease S582A open hexamer with lysozyme -
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Open data
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Basic information
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Title | CryoEM structure of LonC protease S582A open hexamer with lysozyme | |||||||||
![]() | CryoEM map of LonC S582A open hexamer with AGS | |||||||||
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![]() | Lon proteases / chaperone / open hexamer / lysozyme | |||||||||
Function / homology | ![]() endopeptidase La / ATP-dependent peptidase activity / protein catabolic process / serine-type endopeptidase activity / proteolysis / ATP binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.23 Å | |||||||||
![]() | Li M / Hsieh K / Liu H / Zhang S / Gao Y / Gong Q / Zhang K / Chang C / Li S | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Bifurcated assembly pathway and dual function of a Lon-like protease revealed by cryo-EM Analysis Authors: Li M / Liu H / Hsieh KY / Zhang S / Gao Y / Gong Q / Zhang K / Chang CI / Li S | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 136.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.8 KB 16.8 KB | Display Display | ![]() |
Images | ![]() | 46 KB | ||
Filedesc metadata | ![]() | 5.7 KB | ||
Others | ![]() ![]() ![]() | 69.3 MB 134 MB 134 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 14.5 KB | Display | |
Data in CIF | ![]() | 17.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8k93MC ![]() 8k8vC ![]() 8k8wC ![]() 8k8xC ![]() 8k8yC ![]() 8k8zC ![]() 8k90C ![]() 8k91C ![]() 8k92C ![]() 8k94C ![]() 8k95C ![]() 8k96C ![]() 8k97C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | CryoEM map of LonC S582A open hexamer with AGS | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.82 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: CryoEM map of LonC S582A open hexamer with AGS
File | emd_36973_additional_1.map | ||||||||||||
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Annotation | CryoEM map of LonC S582A open hexamer with AGS | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: CryoEM map of LonC S582A open hexamer with AGS
File | emd_36973_half_map_1.map | ||||||||||||
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Annotation | CryoEM map of LonC S582A open hexamer with AGS | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: CryoEM map of LonC S582A open hexamer with AGS
File | emd_36973_half_map_2.map | ||||||||||||
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Annotation | CryoEM map of LonC S582A open hexamer with AGS | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : CryoEM structure of LonC protease S582A open hexamer with lysozyme
Entire | Name: CryoEM structure of LonC protease S582A open hexamer with lysozyme |
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Components |
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-Supramolecule #1: CryoEM structure of LonC protease S582A open hexamer with lysozyme
Supramolecule | Name: CryoEM structure of LonC protease S582A open hexamer with lysozyme type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 480 KDa |
-Macromolecule #1: Endopeptidase La
Macromolecule | Name: Endopeptidase La / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO / EC number: endopeptidase La |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 80.542352 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MRLSYEALEW RTPIENSTEP VSLPPPPPFF GQERAREALE LAIRGGFHAY LVGPPSLGKH EALLAYLSTQ SVETPPDLLY VPLSERKVA VLTLPSGQEI HLAEAVEGLL LEVNRLDELF RQGSFLREKT QLEARFKEAR EQQLEALRRE AQEAGFALST N GERLELTG ...String: MRLSYEALEW RTPIENSTEP VSLPPPPPFF GQERAREALE LAIRGGFHAY LVGPPSLGKH EALLAYLSTQ SVETPPDLLY VPLSERKVA VLTLPSGQEI HLAEAVEGLL LEVNRLDELF RQGSFLREKT QLEARFKEAR EQQLEALRRE AQEAGFALST N GERLELTG PGPVPAELSA RLEEVTLGSL AASAELEVAL RRLRRDWALH YLNNRFEPLF QRFPQARAYL EALRARLARY AE TGEPLDP AQWRPNLLTS SSSGTPPPIV YEPYATAPRL FGRLDYLVDR GVWSTNVSLI RPGAVHRAQG GYLILDALSL KRE GTWEAF KRALRNGQVE PVTEPQAPAG LEVEPFPIQM QVILVGTPEA FEGLEEDPAF SELFRIRAEF SPTLPASPEN CTAL GGWLL AQGFQLTQGG LTRLYDEARR MAEQRDRMDA RLVEIRALAE EAAVLGGGLL TAESVEQAIA AREHRSFLSE EEFLR AVQE GVIRLRTTGR AVGEVNSLVV VEAAPYWGRP ARLTARAAPG RDHLISIDRE AGLGGQIFHK AVLTLAGYLR SRYIEH GSL PVTISLAFEQ NYVSIEGDAA GLAELVAALS AIGNLPLRQD LAVTGAVDQT GKVLAVGAIN AKVEGFFRVC KALGLSG TQ GVILPEANLA NLTLRAEVLE AVRAGQFHIY AVETAEQALE ILAGARMEGF RGLQEKIRAG LEAFARLEEG HDKEDREK L AAALEHHHHH H UniProtKB: endopeptidase La |
-Macromolecule #2: Monothiophosphate
Macromolecule | Name: Monothiophosphate / type: ligand / ID: 2 / Number of copies: 4 / Formula: TS6 |
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Molecular weight | Theoretical: 114.061 Da |
Chemical component information | ![]() ChemComp-TS6: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1.0 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.7 µm / Nominal defocus min: 1.3 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |