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- PDB-8k94: CryoEM structure of LonC protease S582A open pentamer with lysozyme -
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Open data
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Basic information
Entry | Database: PDB / ID: 8k94 | ||||||
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Title | CryoEM structure of LonC protease S582A open pentamer with lysozyme | ||||||
![]() | Endopeptidase La | ||||||
![]() | CHAPERONE / Lon proteases / open pentamer / lysozyme | ||||||
Function / homology | ![]() endopeptidase La / ATP-dependent peptidase activity / protein catabolic process / serine-type endopeptidase activity / proteolysis / ATP binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.4 Å | ||||||
![]() | Li, M. / Hsieh, K. / Liu, H. / Zhang, S. / Gao, Y. / Gong, Q. / Zhang, K. / Chang, C. / Li, S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Bifurcated assembly pathway and dual function of a Lon-like protease revealed by cryo-EM Analysis Authors: Li, M. / Liu, H. / Hsieh, K.Y. / Zhang, S. / Gao, Y. / Gong, Q. / Zhang, K. / Chang, C.I. / Li, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 592.1 KB | Display | ![]() |
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PDB format | ![]() | 498 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 99.4 KB | Display | |
Data in CIF | ![]() | 150.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 36974MC ![]() 8k8vC ![]() 8k8wC ![]() 8k8xC ![]() 8k8yC ![]() 8k8zC ![]() 8k90C ![]() 8k91C ![]() 8k92C ![]() 8k93C ![]() 8k95C ![]() 8k96C ![]() 8k97C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 80542.352 Da / Num. of mol.: 5 / Mutation: S582A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: CryoEM structure of LonC protease S582A open pentamer with lysozyme, AGS Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Value: 0.4 MDa / Experimental value: YES |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.5 |
Specimen | Conc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2700 nm / Nominal defocus min: 1300 nm |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
EM software |
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CTF correction | Type: NONE | ||||||||||||||||||||||||
Particle selection | Num. of particles selected: 951053 | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 76024 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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