+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-36967 | |||||||||
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Title | CryoEM of LonC open pentamer, apo state | |||||||||
Map data | CryoEM map of LonC open pentamer,apo state | |||||||||
Sample |
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Keywords | Lon proteases / chaperone / open pentamer | |||||||||
Function / homology | Function and homology information endopeptidase La / ATP-dependent peptidase activity / protein catabolic process / serine-type endopeptidase activity / proteolysis / ATP binding Similarity search - Function | |||||||||
Biological species | Meiothermus taiwanensis (bacteria) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.53 Å | |||||||||
Authors | Li M / Hsieh K / Liu H / Zhang S / Gao Y / Gong Q / Zhang K / Chang C / Li S | |||||||||
Funding support | China, 1 items
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Citation | Journal: Fundam Res / Year: 2024 Title: Bifurcated assembly pathway and dual function of a Lon-like protease revealed by cryo-EM Analysis Authors: Li M / Liu H / Hsieh KY / Zhang S / Gao Y / Gong Q / Zhang K / Chang CI / Li S | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_36967.map.gz | 136.6 MB | EMDB map data format | |
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Header (meta data) | emd-36967-v30.xml emd-36967.xml | 16.3 KB 16.3 KB | Display Display | EMDB header |
Images | emd_36967.png | 42.7 KB | ||
Filedesc metadata | emd-36967.cif.gz | 5.6 KB | ||
Others | emd_36967_additional_1.map.gz emd_36967_half_map_1.map.gz emd_36967_half_map_2.map.gz | 70.5 MB 134.2 MB 134.2 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-36967 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-36967 | HTTPS FTP |
-Validation report
Summary document | emd_36967_validation.pdf.gz | 1.1 MB | Display | EMDB validaton report |
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Full document | emd_36967_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | emd_36967_validation.xml.gz | 14.3 KB | Display | |
Data in CIF | emd_36967_validation.cif.gz | 16.9 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36967 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-36967 | HTTPS FTP |
-Related structure data
Related structure data | 8k8xMC 8k8vC 8k8wC 8k8yC 8k8zC 8k90C 8k91C 8k92C 8k93C 8k94C 8k95C 8k96C 8k97C M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_36967.map.gz / Format: CCP4 / Size: 144.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | CryoEM map of LonC open pentamer,apo state | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.82 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: CryoEM map of LonC open pentamer,apo state
File | emd_36967_additional_1.map | ||||||||||||
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Annotation | CryoEM map of LonC open pentamer,apo state | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: CryoEM map of LonC open pentamer,apo state
File | emd_36967_half_map_1.map | ||||||||||||
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Annotation | CryoEM map of LonC open pentamer,apo state | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: CryoEM map of LonC open pentamer,apo state
File | emd_36967_half_map_2.map | ||||||||||||
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Annotation | CryoEM map of LonC open pentamer,apo state | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : CryoEM structure of LonC protease hepatmer, apo state
Entire | Name: CryoEM structure of LonC protease hepatmer, apo state |
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Components |
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-Supramolecule #1: CryoEM structure of LonC protease hepatmer, apo state
Supramolecule | Name: CryoEM structure of LonC protease hepatmer, apo state / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Meiothermus taiwanensis (bacteria) |
Molecular weight | Theoretical: 400 KDa |
-Macromolecule #1: endopeptidase La
Macromolecule | Name: endopeptidase La / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO |
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Source (natural) | Organism: Meiothermus taiwanensis (bacteria) |
Molecular weight | Theoretical: 80.558352 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MRLSYEALEW RTPIENSTEP VSLPPPPPFF GQERAREALE LAIRGGFHAY LVGPPSLGKH EALLAYLSTQ SVETPPDLLY VPLSERKVA VLTLPSGQEI HLAEAVEGLL LEVNRLDELF RQGSFLREKT QLEARFKEAR EQQLEALRRE AQEAGFALST N GERLELTG ...String: MRLSYEALEW RTPIENSTEP VSLPPPPPFF GQERAREALE LAIRGGFHAY LVGPPSLGKH EALLAYLSTQ SVETPPDLLY VPLSERKVA VLTLPSGQEI HLAEAVEGLL LEVNRLDELF RQGSFLREKT QLEARFKEAR EQQLEALRRE AQEAGFALST N GERLELTG PGPVPAELSA RLEEVTLGSL AASAELEVAL RRLRRDWALH YLNNRFEPLF QRFPQARAYL EALRARLARY AE TGEPLDP AQWRPNLLTS SSSGTPPPIV YEPYATAPRL FGRLDYLVDR GVWSTNVSLI RPGAVHRAQG GYLILDALSL KRE GTWEAF KRALRNGQVE PVTEPQAPAG LEVEPFPIQM QVILVGTPEA FEGLEEDPAF SELFRIRAEF SPTLPASPEN CTAL GGWLL AQGFQLTQGG LTRLYDEARR MAEQRDRMDA RLVEIRALAE EAAVLGGGLL TAESVEQAIA AREHRSFLSE EEFLR AVQE GVIRLRTTGR AVGEVNSLVV VEAAPYWGRP ARLTARAAPG RDHLISIDRE AGLGGQIFHK AVLTLAGYLR SRYIEH GSL PVTISLAFEQ NYVSIEGDSA GLAELVAALS AIGNLPLRQD LAVTGAVDQT GKVLAVGAIN AKVEGFFRVC KALGLSG TQ GVILPEANLA NLTLRAEVLE AVRAGQFHIY AVETAEQALE ILAGARMEGF RGLQEKIRAG LEAFARLEEG HDKEDREK L AAALEHHHHH H UniProtKB: endopeptidase La |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 1.0 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.7 µm / Nominal defocus min: 1.3 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |