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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | CryoEM map of LonC protease heptamer (heated at 343K) | |||||||||
![]() | CryoEM map of LonC protease (Heat at 343 K) | |||||||||
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![]() | Lon proteases / chaperone / hepatmer / high temperature | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.65 Å | |||||||||
![]() | Li M / Hsieh K / Liu H / Zhang S / Gao Y / Gong Q / Zhang K / Chang C / Li S | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Bifurcated assembly pathway and dual function of a Lon-like protease revealed by cryo-EM Analysis Authors: Li M / Liu H / Hsieh KY / Zhang S / Gao Y / Gong Q / Zhang K / Chang CI / Li S | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 136.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.5 KB 15.5 KB | Display Display | ![]() |
Images | ![]() | 51.8 KB | ||
Filedesc metadata | ![]() | 5.2 KB | ||
Others | ![]() ![]() ![]() | 69.6 MB 133.3 MB 133.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 782.6 KB | Display | ![]() |
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Full document | ![]() | 782.2 KB | Display | |
Data in XML | ![]() | 14.6 KB | Display | |
Data in CIF | ![]() | 17.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8k8vC ![]() 8k8wC ![]() 8k8xC ![]() 8k8yC ![]() 8k8zC ![]() 8k90C ![]() 8k91C ![]() 8k92C ![]() 8k93C ![]() 8k94C ![]() 8k95C ![]() 8k96C ![]() 8k97C C: citing same article ( |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | CryoEM map of LonC protease (Heat at 343 K) | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.2 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: CryoEM map of LonC protease (Heat at 343 K)
File | emd_36979_additional_1.map | ||||||||||||
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Annotation | CryoEM map of LonC protease (Heat at 343 K) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: CryoEM map of LonC protease (Heat at 343 K)
File | emd_36979_half_map_1.map | ||||||||||||
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Annotation | CryoEM map of LonC protease (Heat at 343 K) | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: CryoEM map of LonC protease (Heat at 343 K)
File | emd_36979_half_map_2.map | ||||||||||||
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Annotation | CryoEM map of LonC protease (Heat at 343 K) | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : CryoEM structure of LonC protease hepatmer, heated at 343K
Entire | Name: CryoEM structure of LonC protease hepatmer, heated at 343K |
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Components |
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-Supramolecule #1: CryoEM structure of LonC protease hepatmer, heated at 343K
Supramolecule | Name: CryoEM structure of LonC protease hepatmer, heated at 343K type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 560 KDa |
-Macromolecule #1: LonC heptamer (heated)
Macromolecule | Name: LonC heptamer (heated) / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO |
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Sequence | String: MRLSYEALEW RTPIENSTEP VSLPPPPPFF GQERAREALE LAIRGGFHAY LVGPPSLGKH EALLAYLSTQ SVETPPDLLY VPLSERKVAV LTLPSGQEIH LAEAVEGLLL EVNRLDELFR QGSFLREKTQ LEARFKEARE QQLEALRREA QEAGFALSTN GERLELTGPG ...String: MRLSYEALEW RTPIENSTEP VSLPPPPPFF GQERAREALE LAIRGGFHAY LVGPPSLGKH EALLAYLSTQ SVETPPDLLY VPLSERKVAV LTLPSGQEIH LAEAVEGLLL EVNRLDELFR QGSFLREKTQ LEARFKEARE QQLEALRREA QEAGFALSTN GERLELTGPG PVPAELSARL EEVTLGSLAA SAELEVALRR LRRDWALHYL NNRFEPLFQR FPQARAYLEA LRARLARYAE TGEPLDPAQW RPNLLTSSSS GTPPPIVYEP YATAPRLFGR LDYLVDRGVW STNVSLIRPG AVHRAQGGYL ILDALSLKRE GTWEAFKRAL RNGQVEPVTE PQAPAGLEVE PFPIQMQVIL VGTPEAFEGL EEDPAFSELF RIRAEFSPTL PASPENCTAL GGWLLAQGFQ LTQGGLTRLY DEARRMAEQR DRMDARLVEI RALAEEAAVL GGGLLTAESV EQAIAAREHR SFLSEEEFLR AVQEGVIRLR TTGRAVGEVN SLVVVEAAPY WGRPARLTAR AAPGRDHLIS IDREAGLGGQ IFHKAVLTLA GYLRSRYIEH GSLPVTISLA FEQNYVSIEG DSAGLAELVA ALSAIGNLPL RQDLAVTGAV DQTGKVLAVG AINAKVEGFF RVCKALGLSG TQGVILPEAN LANLTLRAEV LEAVRAGQFH IYAVETAEQA LEILAGARME GFRGLQEKIR AGLEAFARLE EGHDKEDREK LAAALEHHHH HH |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1.0 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS GLACIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 2.0 µm |