National Natural Science Foundation of China (NSFC)
81520108019
中国
National Natural Science Foundation of China (NSFC)
813300237
中国
Ministry of Science and Technology (MoST, China)
2017YFC0840300
中国
引用
ジャーナル: Science / 年: 2020 タイトル: Structure of the RNA-dependent RNA polymerase from COVID-19 virus. 著者: Yan Gao / Liming Yan / Yucen Huang / Fengjiang Liu / Yao Zhao / Lin Cao / Tao Wang / Qianqian Sun / Zhenhua Ming / Lianqi Zhang / Ji Ge / Litao Zheng / Ying Zhang / Haofeng Wang / Yan Zhu / ...著者: Yan Gao / Liming Yan / Yucen Huang / Fengjiang Liu / Yao Zhao / Lin Cao / Tao Wang / Qianqian Sun / Zhenhua Ming / Lianqi Zhang / Ji Ge / Litao Zheng / Ying Zhang / Haofeng Wang / Yan Zhu / Chen Zhu / Tianyu Hu / Tian Hua / Bing Zhang / Xiuna Yang / Jun Li / Haitao Yang / Zhijie Liu / Wenqing Xu / Luke W Guddat / Quan Wang / Zhiyong Lou / Zihe Rao / 要旨: A novel coronavirus [severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2)] outbreak has caused a global coronavirus disease 2019 (COVID-19) pandemic, resulting in tens of thousands of ...A novel coronavirus [severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2)] outbreak has caused a global coronavirus disease 2019 (COVID-19) pandemic, resulting in tens of thousands of infections and thousands of deaths worldwide. The RNA-dependent RNA polymerase [(RdRp), also named nsp12] is the central component of coronaviral replication and transcription machinery, and it appears to be a primary target for the antiviral drug remdesivir. We report the cryo-electron microscopy structure of COVID-19 virus full-length nsp12 in complex with cofactors nsp7 and nsp8 at 2.9-angstrom resolution. In addition to the conserved architecture of the polymerase core of the viral polymerase family, nsp12 possesses a newly identified β-hairpin domain at its N terminus. A comparative analysis model shows how remdesivir binds to this polymerase. The structure provides a basis for the design of new antiviral therapeutics that target viral RdRp.
全体 : Structure of SARS-Cov-2 RNA-dependent RNA polymerase in complex w...
全体
名称: Structure of SARS-Cov-2 RNA-dependent RNA polymerase in complex with cofactors in reduced condition
要素
複合体: Structure of SARS-Cov-2 RNA-dependent RNA polymerase in complex with cofactors in reduced condition
タンパク質・ペプチド: RNA-directed RNA polymerase
タンパク質・ペプチド: Non-structural protein 7
タンパク質・ペプチド: Non-structural protein 8
リガンド: ZINC ION
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超分子 #1: Structure of SARS-Cov-2 RNA-dependent RNA polymerase in complex w...
超分子
名称: Structure of SARS-Cov-2 RNA-dependent RNA polymerase in complex with cofactors in reduced condition タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#3 詳細: full-length 2019-nCoV nsp12 (residues S1-Q932) was incubated with nsp7 (residues S1-Q83) and nsp8 (A1-Q198), and the complex was then purified
由来(天然)
生物種: Severe acute respiratory syndrome coronavirus 2 (ウイルス)