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データを開く
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基本情報
登録情報 | データベース: EMDB / ID: EMD-21050 | |||||||||
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タイトル | Cryo-EM structure of an active human histone pre-mRNA 3'-end processing machinery at 3.2 Angstrom resolution | |||||||||
![]() | active human histone pre-mRNA 3'-end processing machinery | |||||||||
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![]() | endonuclease / active CPSF73 / U7 snRNP / 3'-end processing / RIBONUCLEASE / RNA BINDING PROTEIN-RNA complex | |||||||||
機能・相同性 | ![]() histone mRNA metabolic process / mRNA 3'-end processing by stem-loop binding and cleavage / co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway / 5'-3' RNA exonuclease activity / nuclear stress granule / Processing of Intronless Pre-mRNAs / regulation of chromatin organization / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding ...histone mRNA metabolic process / mRNA 3'-end processing by stem-loop binding and cleavage / co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway / 5'-3' RNA exonuclease activity / nuclear stress granule / Processing of Intronless Pre-mRNAs / regulation of chromatin organization / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / mRNA cleavage and polyadenylation specificity factor complex / histone pre-mRNA 3'end processing complex / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / protein methylation / U12-type spliceosomal complex / mRNA 3'-end processing / methylosome / 7-methylguanosine cap hypermethylation / U1 snRNP binding / mRNA 3'-end processing / pICln-Sm protein complex / Transport of Mature mRNA Derived from an Intronless Transcript / 加水分解酵素; エステル加水分解酵素; 3'-リン酸モノエステル産生エンドリボヌクレアーゼ / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / P granule / telomerase holoenzyme complex / U2-type spliceosomal complex / U2-type precatalytic spliceosome / commitment complex / telomerase RNA binding / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / RNA Polymerase II Transcription Termination / U1 snRNP / U2-type prespliceosome / : / termination of RNA polymerase II transcription / precatalytic spliceosome / spliceosomal complex assembly / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / bicellular tight junction / positive regulation of G1/S transition of mitotic cell cycle / U5 snRNP / spliceosomal snRNP assembly / Cajal body / U4/U6 x U5 tri-snRNP complex / RNA endonuclease activity / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / negative regulation of protein binding / spliceosomal complex / mRNA processing / mRNA splicing, via spliceosome / snRNP Assembly / SARS-CoV-2 modulates host translation machinery / postsynapse / cytoskeleton / nuclear body / cell adhesion / ribonucleoprotein complex / glutamatergic synapse / enzyme binding / RNA binding / nucleoplasm / membrane / nucleus / metal ion binding / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å | |||||||||
![]() | Sun Y / Zhang Y / Walz T / Tong L | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structure of an active human histone pre-mRNA 3'-end processing machinery. 著者: Yadong Sun / Yixiao Zhang / Wei Shen Aik / Xiao-Cui Yang / William F Marzluff / Thomas Walz / Zbigniew Dominski / Liang Tong / ![]() 要旨: The 3'-end processing machinery for metazoan replication-dependent histone precursor messenger RNAs (pre-mRNAs) contains the U7 small nuclear ribonucleoprotein and shares the key cleavage module with ...The 3'-end processing machinery for metazoan replication-dependent histone precursor messenger RNAs (pre-mRNAs) contains the U7 small nuclear ribonucleoprotein and shares the key cleavage module with the canonical cleavage and polyadenylation machinery. We reconstituted an active human histone pre-mRNA processing machinery using 13 recombinant proteins and two RNAs and determined its structure by cryo-electron microscopy. The overall structure is highly asymmetrical and resembles an amphora with one long handle. We captured the pre-mRNA in the active site of the endonuclease, the 73-kilodalton subunit of the cleavage and polyadenylation specificity factor, poised for cleavage. The endonuclease and the entire cleavage module undergo extensive rearrangements for activation, triggered through the recognition of the duplex between the authentic pre-mRNA and U7 small nuclear RNA (snRNA). Our study also has notable implications for understanding canonical and snRNA 3'-end processing. | |||||||||
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構造の表示
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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マップデータ | ![]() | 133.2 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 27.2 KB 27.2 KB | 表示 表示 | ![]() |
画像 | ![]() | 138.9 KB | ||
Filedesc metadata | ![]() | 8.6 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 543.6 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 543.2 KB | 表示 | |
XML形式データ | ![]() | 6.6 KB | 表示 | |
CIF形式データ | ![]() | 7.5 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | active human histone pre-mRNA 3'-end processing machinery | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.07 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
+全体 : The core of metazoan replication-dependent histone pre-mRNA 3'-en...
+超分子 #1: The core of metazoan replication-dependent histone pre-mRNA 3'-en...
+分子 #1: Small nuclear ribonucleoprotein Sm D3
+分子 #2: Small nuclear ribonucleoprotein-associated proteins B and B'
+分子 #3: Small nuclear ribonucleoprotein F
+分子 #4: Small nuclear ribonucleoprotein E
+分子 #5: Small nuclear ribonucleoprotein G
+分子 #6: U7 snRNA-associated Sm-like protein LSm10
+分子 #7: U7 snRNA-associated Sm-like protein LSm11
+分子 #8: Cleavage and polyadenylation specificity factor subunit 3
+分子 #9: Cleavage and polyadenylation specificity factor subunit 2
+分子 #10: Symplekin
+分子 #11: U7 snRNA
+分子 #12: modified H2a pre-mRNA
+分子 #13: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 0.3 mg/mL |
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緩衝液 | pH: 7.5 / 構成要素 - 濃度: 100.0 mM / 構成要素 - 式: NaCl / 構成要素 - 名称: sodium chloride |
グリッド | 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 詳細: unspecified |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 295 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | #0 - Image recording ID: 1 #0 - フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) #0 - 検出モード: COUNTING / #0 - 平均電子線量: 70.0 e/Å2 / #1 - Image recording ID: 2 #1 - フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) #1 - 平均電子線量: 73.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | C2レンズ絞り径: 100.0 µm 最大 デフォーカス(補正後): 2.8000000000000003 µm 最小 デフォーカス(補正後): 0.9 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.2 µm / 倍率(公称値): 22500 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |