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Open data
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Basic information
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Title | Structure of the human CCAN deltaCT complex | ||||||||||||
![]() | 3D consensus refinement | ||||||||||||
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Function / homology | ![]() positive regulation of protein localization to kinetochore / Mis6-Sim4 complex / centromere complex assembly / kinetochore organization / metaphase chromosome alignment / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() | ||||||||||||
![]() | Muir KW / Yatskevich S / Bellini D / Barford D | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome. Authors: Stanislau Yatskevich / Kyle W Muir / Dom Bellini / Ziguo Zhang / Jing Yang / Thomas Tischer / Masa Predin / Tom Dendooven / Stephen H McLaughlin / David Barford / ![]() Abstract: Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron ...Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-A reconstituted onto α-satellite DNA. CCAN forms edge-on contacts with CENP-A, and a linker DNA segment of the α-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle. | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 15.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 31.2 KB 31.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 12.1 KB | Display | ![]() |
Images | ![]() | 52.5 KB | ||
Others | ![]() ![]() ![]() ![]() | 9.9 MB 9.4 MB 10.8 MB 116.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7pknMC ![]() 7pb4C ![]() 7pb8C ![]() 7piiC ![]() 7r5rC ![]() 7r5sC ![]() 7r5vC ![]() 7ywxC ![]() 7yyhC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | 3D consensus refinement | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.86 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: HIKMLN body
File | emd_13473_additional_1.map | ||||||||||||
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Annotation | HIKMLN body | ||||||||||||
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Density Histograms |
-Additional map: OPQURN body
File | emd_13473_additional_2.map | ||||||||||||
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Annotation | OPQURN body | ||||||||||||
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-Additional map: OPQURN focused refinement
File | emd_13473_additional_3.map | ||||||||||||
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Annotation | OPQURN focused refinement | ||||||||||||
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-Additional map: Combined focused map from PHENIX
File | emd_13473_additional_4.map | ||||||||||||
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Annotation | Combined focused map from PHENIX | ||||||||||||
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Sample components
+Entire : Human inner kinetochore CCAN
+Supramolecule #1: Human inner kinetochore CCAN
+Macromolecule #1: Centromere protein H
+Macromolecule #2: Centromere protein I
+Macromolecule #3: Centromere protein K
+Macromolecule #4: Centromere protein L
+Macromolecule #5: Centromere protein M
+Macromolecule #6: Centromere protein N
+Macromolecule #7: Centromere protein O
+Macromolecule #8: Centromere protein P
+Macromolecule #9: Centromere protein Q
+Macromolecule #10: Centromere protein U
+Macromolecule #11: Centromere protein R
+Macromolecule #12: water
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |