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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-13193 | |||||||||
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Title | Structure of human ASCT1 transporter | |||||||||
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![]() | Solute carrier / membrane protein / amino acid transport / TRANSPORT PROTEIN | |||||||||
Function / homology | ![]() L-threonine transmembrane transporter activity / L-alanine transport / L-serine transport / threonine transport / hydroxyproline transport / L-hydroxyproline transmembrane transporter activity / L-serine import across plasma membrane / L-proline transmembrane transporter activity / L-cystine transmembrane transporter activity / L-cystine transport ...L-threonine transmembrane transporter activity / L-alanine transport / L-serine transport / threonine transport / hydroxyproline transport / L-hydroxyproline transmembrane transporter activity / L-serine import across plasma membrane / L-proline transmembrane transporter activity / L-cystine transmembrane transporter activity / L-cystine transport / glutamine transport / L-glutamine transmembrane transporter activity / L-serine transmembrane transporter activity / L-alanine transmembrane transporter activity / L-alanine import across plasma membrane / proline transport / L-glutamate transmembrane transport / amino acid transmembrane transporter activity / L-aspartate transmembrane transporter activity / Amino acid transport across the plasma membrane / L-aspartate import across plasma membrane / neutral L-amino acid transmembrane transporter activity / symporter activity / intermediate filament / chloride channel activity / amino acid transport / plasma membrane => GO:0005886 / transport across blood-brain barrier / synaptic transmission, glutamatergic / cognition / melanosome / membrane => GO:0016020 / centrosome / neuronal cell body / synapse / dendrite / cell surface / extracellular exosome / membrane / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.2 Å | |||||||||
![]() | Stetsenko A / Stehantsev P | |||||||||
![]() | ![]() Title: A structural view onto disease-linked mutations in the human neutral amino acid exchanger ASCT1. Authors: Pavlo Stehantsev / Artem Stetsenko / Mariia Nemchinova / Nanda Gowtham Aduri / Siewert J Marrink / Cornelius Gati / Albert Guskov / ![]() ![]() ![]() Abstract: The ASCT1 transporter of the SLC1 family is largely involved in equilibration of neutral amino acids' pools across the plasma membrane and plays a prominent role in the transport of both L- and D- ...The ASCT1 transporter of the SLC1 family is largely involved in equilibration of neutral amino acids' pools across the plasma membrane and plays a prominent role in the transport of both L- and D-isomers of serine, essential for the normal functioning of the central nervous system in mammals. A number of mutations in ASCT1 (E256K, G381R, R457W) have been linked to severe neurodevelopmental disorders, however in the absence of ASCT1 structure it is hard to understand their impact on substrate transport. To ameliorate that we have determined a cryo-EM structure of human ASCT1 at 4.2 Å resolution and performed functional transport assays and molecular dynamics simulations, which revealed that given mutations lead to the diminished transport capability of ASCT1 caused by instability of transporter and impeded transport cycle. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 59.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 8.9 KB 8.9 KB | Display Display | ![]() |
Images | ![]() | 84.4 KB | ||
Filedesc metadata | ![]() | 4.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 410.8 KB | Display | ![]() |
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Full document | ![]() | 410.3 KB | Display | |
Data in XML | ![]() | 6.9 KB | Display | |
Data in CIF | ![]() | 7.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7p4iMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.8521 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : trimeric ASCT1 transporter
Entire | Name: trimeric ASCT1 transporter |
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Components |
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-Supramolecule #1: trimeric ASCT1 transporter
Supramolecule | Name: trimeric ASCT1 transporter / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 167 KDa |
-Macromolecule #1: Neutral amino acid transporter A
Macromolecule | Name: Neutral amino acid transporter A / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 55.76666 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MEKSNETNGY LDSAQAGPAA GPGAPGTAAG RARRCAGFLR RQALVLLTVS GVLAGAGLGA ALRGLSLSRT QVTYLAFPGE MLLRMLRMI ILPLVVCSLV SGAASLDASC LGRLGGIAVA YFGLTTLSAS ALAVALAFII KPGSGAQTLQ SSDLGLEDSG P PPVPKETV ...String: MEKSNETNGY LDSAQAGPAA GPGAPGTAAG RARRCAGFLR RQALVLLTVS GVLAGAGLGA ALRGLSLSRT QVTYLAFPGE MLLRMLRMI ILPLVVCSLV SGAASLDASC LGRLGGIAVA YFGLTTLSAS ALAVALAFII KPGSGAQTLQ SSDLGLEDSG P PPVPKETV DSFLDLARNL FPSNLVVAAF RTYATDYKVV TQNSSSGNVT HEKIPIGTEI EGMNILGLVL FALVLGVALK KL GSEGEDL IRFFNSLNEA TMVLVSWIMW YVPVGIMFLV GSKIVEMKDI IVLVTSLGKY IFASILGHVI HGGIVLPLIY FVF TRKNPF RFLLGLLAPF ATAFATCSSS ATLPSMMKCI EENNGVDKRI SRFILPIGAT VNMDGAAIFQ CVAAVFIAQL NNVE LNAGQ IFTILVTATA SSVGAAGVPA GGVLTIAIIL EAIGLPTHDL PLILAVDWIV DRTTTVVNVE GDALGAGILH HLNQK ATKK GEQELAEVKV EAIPNCKSEE ETSPLVTHQN PAGPVASAPE LESKESVL UniProtKB: Neutral amino acid transporter A |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: OTHER |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 134344 |
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |