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- EMDB-13144: Cryo-EM structure of Pdr5 from Saccharomyces cerevisiae in inward... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-13144 | |||||||||
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Title | Cryo-EM structure of Pdr5 from Saccharomyces cerevisiae in inward-facing conformation with ADP/ATP and rhodamine 6G | |||||||||
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Function / homology | ![]() intracellular monoatomic cation homeostasis / xenobiotic detoxification by transmembrane export across the plasma membrane / ABC-type xenobiotic transporter activity / response to cycloheximide / cell periphery / response to xenobiotic stimulus / response to antibiotic / ATP hydrolysis activity / mitochondrion / ATP binding ...intracellular monoatomic cation homeostasis / xenobiotic detoxification by transmembrane export across the plasma membrane / ABC-type xenobiotic transporter activity / response to cycloheximide / cell periphery / response to xenobiotic stimulus / response to antibiotic / ATP hydrolysis activity / mitochondrion / ATP binding / identical protein binding / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.13 Å | |||||||||
![]() | Szewczak-Harris A / Wagner M / Du D / Schmitt L / Luisi BF | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and efflux mechanism of the yeast pleiotropic drug resistance transporter Pdr5. Authors: Andrzej Harris / Manuel Wagner / Dijun Du / Stefanie Raschka / Lea-Marie Nentwig / Holger Gohlke / Sander H J Smits / Ben F Luisi / Lutz Schmitt / ![]() ![]() ![]() Abstract: Pdr5, a member of the extensive ABC transporter superfamily, is representative of a clinically relevant subgroup involved in pleiotropic drug resistance. Pdr5 and its homologues drive drug efflux ...Pdr5, a member of the extensive ABC transporter superfamily, is representative of a clinically relevant subgroup involved in pleiotropic drug resistance. Pdr5 and its homologues drive drug efflux through uncoupled hydrolysis of nucleotides, enabling organisms such as baker's yeast and pathogenic fungi to survive in the presence of chemically diverse antifungal agents. Here, we present the molecular structure of Pdr5 solved with single particle cryo-EM, revealing details of an ATP-driven conformational cycle, which mechanically drives drug translocation through an amphipathic channel, and a clamping switch within a conserved linker loop that acts as a nucleotide sensor. One half of the transporter remains nearly invariant throughout the cycle, while its partner undergoes changes that are transmitted across inter-domain interfaces to support a peristaltic motion of the pumped molecule. The efflux model proposed here rationalises the pleiotropic impact of Pdr5 and opens new avenues for the development of effective antifungal compounds. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 500.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.4 KB 15.4 KB | Display Display | ![]() |
Images | ![]() | 49.1 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7p05MC ![]() 7p03C ![]() 7p04C ![]() 7p06C M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.652 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Cryo-EM structure of Pdr5 from Saccharomyces cerevisiae in inward...
Entire | Name: Cryo-EM structure of Pdr5 from Saccharomyces cerevisiae in inward-facing conformation with ADP/ATP and rhodamine 6G |
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Components |
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-Supramolecule #1: Cryo-EM structure of Pdr5 from Saccharomyces cerevisiae in inward...
Supramolecule | Name: Cryo-EM structure of Pdr5 from Saccharomyces cerevisiae in inward-facing conformation with ADP/ATP and rhodamine 6G type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() Location in cell: cell membrane |
-Macromolecule #1: Pleiotropic ABC efflux transporter of multiple drugs
Macromolecule | Name: Pleiotropic ABC efflux transporter of multiple drugs / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 170.61725 KDa |
Sequence | String: MPEAKLNNNV NDVTSYSSAS SSTENAADLH NYNGFDEHTE ARIQKLARTL TAQSMQNSTQ SAPNKSDAQS IFSSGVEGVN PIFSDPEAP GYDPKLDPNS ENFSSAAWVK NMAHLSAADP DFYKPYSLGC AWKNLSASGA SADVAYQSTV VNIPYKILKS G LRKFQRSK ...String: MPEAKLNNNV NDVTSYSSAS SSTENAADLH NYNGFDEHTE ARIQKLARTL TAQSMQNSTQ SAPNKSDAQS IFSSGVEGVN PIFSDPEAP GYDPKLDPNS ENFSSAAWVK NMAHLSAADP DFYKPYSLGC AWKNLSASGA SADVAYQSTV VNIPYKILKS G LRKFQRSK ETNTFQILKP MDGCLNPGEL LVVLGRPGSG CTTLLKSISS NTHGFDLGAD TKISYSGYSG DDIKKHFRGE VV YNAEADV HLPHLTVFET LVTVARLKTP QNRIKGVDRE SYANHLAEVA MATYGLSHTR NTKVGNDIVR GVSGGERKRV SIA EVSICG SKFQCWDNAT RGLDSATALE FIRALKTQAD ISNTSATVAI YQCSQDAYDL FNKVCVLDDG YQIYYGPADK AKKY FEDMG YVCPSRQTTA DFLTSVTSPS ERTLNKDMLK KGIHIPQTPK EMNDYWVKSP NYKELMKEVD QRLLNDDEAS REAIK EAHI AKQSKRARPS SPYTVSYMMQ VKYLLIRNMW RLRNNIGFTL FMILGNCSMA LILGSMFFKI MKKGDTSTFY FRGSAM FFA ILFNAFSSLL EIFSLYEARP ITEKHRTYSL YHPSADAFAS VLSEIPSKLI IAVCFNIIFY FLVDFRRNGG VFFFYLL IN IVAVFSMSHL FRCVGSLTKT LSEAMVPASM LLLALSMYTG FAIPKKKILR WSKWIWYINP LAYLFESLLI NEFHGIKF P CAEYVPRGPA YANISSTESV CTVVGAVPGQ DYVLGDDFIR GTYQYYHKDK WRGFGIGMAY VVFFFFVYLF LCEYNEGAK QKGEILVFPR SIVKRMKKRG VLTEKNANDP ENVGERSDLS SDRKMLQESS EEESDTYGEI GLSKSEAIFH WRNLCYEVQI KAETRRILN NVDGWVKPGT LTALMGASGA GKTTLLDCLA ERVTMGVITG DILVNGIPRD KSFPRSIGYC QQQDLHLKTA T VRESLRFS AYLRQPAEVS IEEKNRYVEE VIKILEMEKY ADAVVGVAGE GLNVEQRKRL TIGVELTAKP KLLVFLDEPT SG LDSQTAW SICQLMKKLA NHGQAILCTI HQPSAILMQE FDRLLFMQRG GKTVYFGDLG EGCKTMIDYF ESHGAHKCPA DAN PAEWML EVVGAAPGSH ANQDYYEVWR NSEEYRAVQS ELDWMERELP KKGSITAAED KHEFSQSIIY QTKLVSIRLF QQYW RSPDY LWSKFILTIF NQLFIGFTFF KAGTSLQGLQ NQMLAVFMFT VIFNPILQQY LPSFVQQRDL YEARERPSRT FSWIS FIFA QIFVEVPWNI LAGTIAYFIY YYPIGFYSNA SAAGQLHERG ALFWLFSCAF YVYVGSMGLL VISFNQVAES AANLAS LLF TMSLSFCGVM TTPSAMPRFW IFMYRVSPLT YFIQALLAVG VANVDVKCAD YELLEFTPPS GMTCGQYMEP YLQLAKT GY LTDENATDTC SFCQISTTND YLANVNSFYS ERWRNYGIFI CYIAFNYIAG VFFYWLARVP KKNGKLSKK |
-Macromolecule #2: ADENOSINE-5'-TRIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 2 / Number of copies: 1 / Formula: ATP |
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Molecular weight | Theoretical: 507.181 Da |
Chemical component information | ![]() ChemComp-ATP: |
-Macromolecule #3: ADENOSINE-5'-DIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 3 / Number of copies: 1 / Formula: ADP |
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Molecular weight | Theoretical: 427.201 Da |
Chemical component information | ![]() ChemComp-ADP: |
-Macromolecule #4: RHODAMINE 6G
Macromolecule | Name: RHODAMINE 6G / type: ligand / ID: 4 / Number of copies: 1 / Formula: RHQ |
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Molecular weight | Theoretical: 443.557 Da |
Chemical component information | ![]() ChemComp-R6G: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 2.5 mg/mL | |||||||||
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Buffer | pH: 7.8 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE | |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average exposure time: 1.31 sec. / Average electron dose: 47.73 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.7000000000000001 µm / Nominal magnification: 130000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: HELIUM |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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Output model | ![]() PDB-7p05: |