Biotechnology and Biological Sciences Research Council (BBSRC)
BB/N002954/1
英国
Medical Research Council (MRC, United Kingdom)
MC_PC_171136
英国
引用
ジャーナル: Nucleic Acids Res / 年: 2015 タイトル: Structural and functional characterization of a cell cycle associated HDAC1/2 complex reveals the structural basis for complex assembly and nucleosome targeting. 著者: Toshimasa Itoh / Louise Fairall / Frederick W Muskett / Charles P Milano / Peter J Watson / Nadia Arnaudo / Almutasem Saleh / Christopher J Millard / Mohammed El-Mezgueldi / Fabrizio Martino / John W R Schwabe / 要旨: Recent proteomic studies have identified a novel histone deacetylase complex that is upregulated during mitosis and is associated with cyclin A. This complex is conserved from nematodes to man and ...Recent proteomic studies have identified a novel histone deacetylase complex that is upregulated during mitosis and is associated with cyclin A. This complex is conserved from nematodes to man and contains histone deacetylases 1 and 2, the MIDEAS corepressor protein and a protein called DNTTIP1 whose function was hitherto poorly understood. Here, we report the structures of two domains from DNTTIP1. The amino-terminal region forms a tight dimerization domain with a novel structural fold that interacts with and mediates assembly of the HDAC1:MIDEAS complex. The carboxy-terminal domain of DNTTIP1 has a structure related to the SKI/SNO/DAC domain, despite lacking obvious sequence homology. We show that this domain in DNTTIP1 mediates interaction with both DNA and nucleosomes. Thus, DNTTIP1 acts as a dimeric chromatin binding module in the HDAC1:MIDEAS corepressor complex.
全体 : Tetrameric complex of the MiDAC deacetylase complex containing HD...
全体
名称: Tetrameric complex of the MiDAC deacetylase complex containing HDAC1, the ELM2-SANT domain of MIDEAS and the dimerisation domain of DNTTIP1
要素
複合体: Tetrameric complex of the MiDAC deacetylase complex containing HDAC1, the ELM2-SANT domain of MIDEAS and the dimerisation domain of DNTTIP1
タンパク質・ペプチド: Histone deacetylase 1
タンパク質・ペプチド: Deoxynucleotidyltransferase terminal-interacting protein 1
タンパク質・ペプチド: Mitotic deacetylase-associated SANT domain protein
リガンド: ZINC ION
リガンド: POTASSIUM ION
リガンド: INOSITOL HEXAKISPHOSPHATE
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超分子 #1: Tetrameric complex of the MiDAC deacetylase complex containing HD...
超分子
名称: Tetrameric complex of the MiDAC deacetylase complex containing HDAC1, the ELM2-SANT domain of MIDEAS and the dimerisation domain of DNTTIP1 タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#3