+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-10769 | ||||||||||||
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タイトル | Structure of a human 48S translational initiation complex - eIF3 | ||||||||||||
マップデータ | |||||||||||||
試料 |
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キーワード | eIF3 / ribosome / translation / initiation complex | ||||||||||||
機能・相同性 | 機能・相同性情報 positive regulation of mRNA binding / viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / IRES-dependent viral translational initiation / multi-eIF complex / formation of cytoplasmic translation initiation complex / eukaryotic translation initiation factor 3 complex ...positive regulation of mRNA binding / viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / translation reinitiation / IRES-dependent viral translational initiation / multi-eIF complex / formation of cytoplasmic translation initiation complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / cytoplasmic translational initiation / mRNA cap binding / eukaryotic 48S preinitiation complex / negative regulation of RNA splicing / metal-dependent deubiquitinase activity / neural crest cell differentiation / regulation of translational initiation / positive regulation of ubiquitin-protein transferase activity / rRNA modification in the nucleus and cytosol / Formation of the ternary complex, and subsequently, the 43S complex / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / laminin receptor activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / negative regulation of ubiquitin protein ligase activity / Ribosomal scanning and start codon recognition / Translation initiation complex formation / fibroblast growth factor binding / SARS-CoV-1 modulates host translation machinery / Peptide chain elongation / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / Selenocysteine synthesis / Formation of a pool of free 40S subunits / Eukaryotic Translation Termination / ubiquitin ligase inhibitor activity / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of ubiquitin-dependent protein catabolic process / Protein methylation / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / translation regulator activity / Mitotic Prometaphase / cytosolic ribosome / laminin binding / EML4 and NUDC in mitotic spindle formation / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rough endoplasmic reticulum / antiviral innate immune response / negative regulation of translational initiation / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / translation initiation factor binding / Resolution of Sister Chromatid Cohesion / translation initiation factor activity / erythrocyte differentiation / maturation of SSU-rRNA / neural tube closure / small-subunit processome / mRNA 3'-UTR binding / positive regulation of translation / translational initiation / RHO GTPases Activate Formins / PML body / negative regulation of ERK1 and ERK2 cascade / RMTs methylate histone arginines / receptor tyrosine kinase binding / fibrillar center / mRNA 5'-UTR binding / Regulation of expression of SLITs and ROBOs / rRNA processing / Separation of Sister Chromatids / metallopeptidase activity / virus receptor activity / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / SARS-CoV-2 modulates host translation machinery / cytosolic small ribosomal subunit / ubiquitinyl hydrolase 1 / microtubule / cysteine-type deubiquitinase activity / cytoplasmic translation / cell differentiation / postsynaptic density / protein stabilization / ribosome / structural constituent of ribosome / cadherin binding / translation / ribonucleoprotein complex / focal adhesion 類似検索 - 分子機能 | ||||||||||||
生物種 | Homo sapiens (ヒト) | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.3 Å | ||||||||||||
データ登録者 | Brito Querido J / Sokabe M | ||||||||||||
資金援助 | 英国, 米国, 3件
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引用 | ジャーナル: Science / 年: 2020 タイトル: Structure of a human 48 translational initiation complex. 著者: Jailson Brito Querido / Masaaki Sokabe / Sebastian Kraatz / Yuliya Gordiyenko / J Mark Skehel / Christopher S Fraser / V Ramakrishnan / 要旨: A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to ...A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to form the 48 initiation complex (i.e., the 48). The 48 then scans along the mRNA to locate a start codon. To understand the mechanisms involved, we used cryo-electron microscopy to determine the structure of a reconstituted human 48 The structure reveals insights into early events of translation initiation complex assembly, as well as how eIF4F interacts with subunits of eIF3 near the mRNA exit channel in the 43 The location of eIF4F is consistent with a slotting model of mRNA recruitment and suggests that downstream mRNA is unwound at least in part by being "pulled" through the 40 subunit during scanning. | ||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_10769.map.gz | 11.4 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-10769-v30.xml emd-10769.xml | 37.9 KB 37.9 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_10769_fsc.xml | 17.7 KB | 表示 | FSCデータファイル |
画像 | emd_10769.png | 56 KB | ||
マスクデータ | emd_10769_msk_1.map | 83.7 MB | マスクマップ | |
Filedesc metadata | emd-10769.cif.gz | 10 KB | ||
その他 | emd_10769_additional.map.gz emd_10769_half_map_1.map.gz emd_10769_half_map_2.map.gz | 64.3 MB 64.6 MB 64.6 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-10769 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-10769 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_10769_validation.pdf.gz | 422.2 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_10769_full_validation.pdf.gz | 421.4 KB | 表示 | |
XML形式データ | emd_10769_validation.xml.gz | 18.9 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10769 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10769 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_10769.map.gz / 形式: CCP4 / 大きさ: 83.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.074 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-マスク #1
ファイル | emd_10769_msk_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-追加マップ: #1
ファイル | emd_10769_additional.map | ||||||||||||
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_10769_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #2
ファイル | emd_10769_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : 48S initiation complex
+超分子 #1: 48S initiation complex
+分子 #1: Eukaryotic translation initiation factor 3 subunit M
+分子 #2: Eukaryotic translation initiation factor 3 subunit F
+分子 #3: Eukaryotic translation initiation factor 3 subunit A
+分子 #4: Eukaryotic translation initiation factor 3 subunit E
+分子 #5: Eukaryotic translation initiation factor 3 subunit C
+分子 #6: Eukaryotic translation initiation factor 3 subunit H
+分子 #7: 40S ribosomal protein S7
+分子 #8: 40S ribosomal protein S27
+分子 #9: 40S ribosomal protein S21
+分子 #10: 40S ribosomal protein S17
+分子 #11: 40S ribosomal protein S2
+分子 #12: 40S ribosomal protein S3a
+分子 #13: 40S ribosomal protein SA
+分子 #14: 40S ribosomal protein S26
+分子 #15: 40S ribosomal protein S14
+分子 #16: 40S ribosomal protein S13
+分子 #17: Eukaryotic translation initiation factor 3 subunit D
+分子 #18: Eukaryotic translation initiation factor 3 subunit K
+分子 #19: Eukaryotic translation initiation factor 3 subunit L
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: INTEGRATING / 平均露光時間: 1.0 sec. / 平均電子線量: 107.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |