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TitleStructure of a human 48 translational initiation complex.
Journal, issue, pagesScience, Vol. 369, Issue 6508, Page 1220-1227, Year 2020
Publish dateSep 4, 2020
AuthorsJailson Brito Querido / Masaaki Sokabe / Sebastian Kraatz / Yuliya Gordiyenko / J Mark Skehel / Christopher S Fraser / V Ramakrishnan /
PubMed AbstractA key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to ...A key step in translational initiation is the recruitment of the 43 preinitiation complex by the cap-binding complex [eukaryotic initiation factor 4F (eIF4F)] at the 5' end of messenger RNA (mRNA) to form the 48 initiation complex (i.e., the 48). The 48 then scans along the mRNA to locate a start codon. To understand the mechanisms involved, we used cryo-electron microscopy to determine the structure of a reconstituted human 48 The structure reveals insights into early events of translation initiation complex assembly, as well as how eIF4F interacts with subunits of eIF3 near the mRNA exit channel in the 43 The location of eIF4F is consistent with a slotting model of mRNA recruitment and suggests that downstream mRNA is unwound at least in part by being "pulled" through the 40 subunit during scanning.
External linksScience / PubMed:32883864 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 6.0 Å
Structure data

10769

6ybd

EMDB-10769, PDB-6ybd:
Structure of a human 48S translational initiation complex - eIF3
Method: EM (single particle) / Resolution: 3.3 Å

10772

6ybs

EMDB-10772, PDB-6ybs:
Structure of a human 48S translational initiation complex - head
Method: EM (single particle) / Resolution: 3.1 Å

10773

6ybt

EMDB-10773, PDB-6ybt:
Structure of a human 48S translational initiation complex - eIF3bgi
Method: EM (single particle) / Resolution: 6.0 Å

10774

6ybv

EMDB-10774, PDB-6ybv:
Structure of a human 48S translational initiation complex - eIF2-TC
Method: EM (single particle) / Resolution: 3.8 Å

10775

6ybw

EMDB-10775, PDB-6ybw:
Structure of a human 48S translational initiation complex - 40S body
Method: EM (single particle) / Resolution: 3.1 Å

11302

6zmw

EMDB-11302, PDB-6zmw:
Structure of a human 48S translational initiation complex
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • Human (human)
KeywordsTRANSLATION / eIF3 / ribosome / initiation complex

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