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- EMDB-8149: Cryo-EM structure of a full archaeal ribosomal translation initia... -

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Basic information

Entry
Database: EMDB / ID: EMD-8149
TitleCryo-EM structure of a full archaeal ribosomal translation initiation complex in the P-IN conformation
Map dataNone
Sample
  • Complex: 30S archaeal translation initiation complex
    • Complex: 30S archaeal translation initiation complex
      • RNA: x 1 types
      • Protein or peptide: x 28 types
    • Complex: 30S archaeal translation initiation complex
      • Protein or peptide: x 5 types
    • Complex: 30S archaeal translation initiation complex
      • RNA: x 1 types
    • Complex: 30S archaeal translation initiation complex
      • RNA: x 1 types
  • Ligand: x 4 types
Function / homology
Function and homology information


translation reinitiation / protein-synthesizing GTPase / formation of cytoplasmic translation initiation complex / formation of translation preinitiation complex / ribonuclease P activity / tRNA 5'-leader removal / translation elongation factor activity / translational initiation / translation initiation factor activity / cytosolic ribosome ...translation reinitiation / protein-synthesizing GTPase / formation of cytoplasmic translation initiation complex / formation of translation preinitiation complex / ribonuclease P activity / tRNA 5'-leader removal / translation elongation factor activity / translational initiation / translation initiation factor activity / cytosolic ribosome / ribosome binding / ribosome biogenesis / small ribosomal subunit / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / GTPase activity / GTP binding / RNA binding / zinc ion binding / metal ion binding / cytoplasm
Similarity search - Function
Translation factor SUI1 homolog, archaea / Archaeal/bacterial translation initiation factor SUI1 / Translation initiation factor 2, alpha subunit, archaeal / Translation initiation factor 2, beta subunit / Ribosomal protein S27ae / Translation initiation factor 2, gamma subunit / : / Ribosomal protein S9, archaeal / Ribosomal protein S13, archaeal / Ribosomal protein S17, archaeal ...Translation factor SUI1 homolog, archaea / Archaeal/bacterial translation initiation factor SUI1 / Translation initiation factor 2, alpha subunit, archaeal / Translation initiation factor 2, beta subunit / Ribosomal protein S27ae / Translation initiation factor 2, gamma subunit / : / Ribosomal protein S9, archaeal / Ribosomal protein S13, archaeal / Ribosomal protein S17, archaeal / Ribosomal protein S6e, archaeal / Ribosomal protein S4, archaeal / Ribosomal protein S12, archaea / Ribosomal protein S3, archaeal / 30S ribosomal protein S3Ae / Ribosomal protein S7, archaeal / Ribosomal protein S19e, archaeal / Ribosomal protein S11, archaeal / Ribosomal protein S2, archaeal / Ribosomal protein S14, type Z, archaeal / Ribosomal protein S8e, archaeal / SUI1 domain superfamily / Translation initiation factor SUI1 / Translation initiation factor SUI1 family profile. / SUI1 domain / Translation initiation factor IF2/IF5 / Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / IF2a, S1-like domain / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / Eukaryotic translation initiation factor 2 alpha subunit / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, C terminal / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / Ribosomal protein L7Ae, archaea / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / Ribosomal protein S19e, conserved site / S1 domain profile. / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S17e, conserved site / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19A/S15e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal S17 / Ribosomal protein S19e signature. / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / Ribosomal protein S4e, N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein S8e / Ribosomal protein S4, KOW domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / RS4NT (NUC023) domain / Ribosomal protein S27 / Ribosomal S3Ae family / Ribosomal protein S17e signature. / Ribosomal protein S28e / Ribosomal family S4e / Ribosomal S13/S15 N-terminal domain
Similarity search - Domain/homology
Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein eL8 / Protein translation factor SUI1 homolog / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS17 ...Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein eL8 / Protein translation factor SUI1 homolog / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS32 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS24 / Small ribosomal subunit protein eS31 / Small ribosomal subunit protein eS27 / Translation initiation factor 2 subunit beta / Translation initiation factor 2 subunit alpha / Translation initiation factor 2 subunit gamma / Small ribosomal subunit protein eS19 / Translation initiation factor 1A
Similarity search - Component
Biological speciesPyrococcus abyssi GE5 (archaea) / Escherichia coli (E. coli) / Pyrococcus (archaea)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.34 Å
AuthorsCoureux P-D / Schmitt E / Mechulam Y
CitationJournal: Nat Commun / Year: 2016
Title: Cryo-EM study of start codon selection during archaeal translation initiation.
Authors: Pierre-Damien Coureux / Christine Lazennec-Schurdevin / Auriane Monestier / Eric Larquet / Lionel Cladière / Bruno P Klaholz / Emmanuelle Schmitt / Yves Mechulam /
Abstract: Eukaryotic and archaeal translation initiation complexes have a common structural core comprising e/aIF1, e/aIF1A, the ternary complex (TC, e/aIF2-GTP-Met-tRNA) and mRNA bound to the small ribosomal ...Eukaryotic and archaeal translation initiation complexes have a common structural core comprising e/aIF1, e/aIF1A, the ternary complex (TC, e/aIF2-GTP-Met-tRNA) and mRNA bound to the small ribosomal subunit. e/aIF2 plays a crucial role in this process but how this factor controls start codon selection remains unclear. Here, we present cryo-EM structures of the full archaeal 30S initiation complex showing two conformational states of the TC. In the first state, the TC is bound to the ribosome in a relaxed conformation with the tRNA oriented out of the P site. In the second state, the tRNA is accommodated within the peptidyl (P) site and the TC becomes constrained. This constraint is compensated by codon/anticodon base pairing, whereas in the absence of a start codon, aIF2 contributes to swing out the tRNA. This spring force concept highlights a mechanism of codon/anticodon probing by the initiator tRNA directly assisted by aIF2.
History
DepositionApr 13, 2016-
Header (metadata) releaseDec 7, 2016-
Map releaseDec 7, 2016-
UpdateDec 11, 2019-
Current statusDec 11, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.015
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.015
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5jbh
  • Surface level: 0.015
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8149.png

Downloads & links

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Map

FileDownload / File: emd_8149.map.gz / Format: CCP4 / Size: 160.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationNone
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.12 Å/pix.
x 348 pix.
= 389.76 Å		1.12 Å/pix.
x 348 pix.
= 389.76 Å		1.12 Å/pix.
x 348 pix.
= 389.76 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.12 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.015 / Movie #1: 0.015
Minimum - Maximum-0.11587982 - 0.18483302
Average (Standard dev.)0.0010275262 (±0.008826057)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions348348348
Spacing348348348
CellA=B=C: 389.76 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.121.121.12
M x/y/z348348348
origin x/y/z0.0000.0000.000
length x/y/z389.760389.760389.760
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS348348348
D min/max/mean-0.1160.1850.001

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Supplemental data

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Sample components

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Entire : 30S archaeal translation initiation complex

EntireName: 30S archaeal translation initiation complex
Components
  • Complex: 30S archaeal translation initiation complex
    • Complex: 30S archaeal translation initiation complex
      • RNA: 16S ribosomal RNA
      • Protein or peptide: 30S ribosomal protein uS3
      • Protein or peptide: 50S ribosomal protein uL30
      • Protein or peptide: 30S ribosomal protein uS10
      • Protein or peptide: 30S ribosomal protein uS13
      • Protein or peptide: 30S ribosomal protein uS14
      • Protein or peptide: 30S ribosomal protein eS17
      • Protein or peptide: 30S ribosomal protein uS19
      • Protein or peptide: 30S ribosomal protein eS19
      • Protein or peptide: 30S ribosomal protein eS28
      • Protein or peptide: 30S ribosomal protein eS27
      • Protein or peptide: 30S ribosomal protein uS7
      • Protein or peptide: 30S ribosomal protein uS9
      • Protein or peptide: 30S ribosomal protein uS11
      • Protein or peptide: 30S ribosomal protein uS12
      • Protein or peptide: 30S ribosomal protein uS15
      • Protein or peptide: 30S ribosomal protein uS17
      • Protein or peptide: 30S ribosomal protein uS3
      • Protein or peptide: 30S ribosomal protein uS2
      • Protein or peptide: 30S ribosomal protein eS24
      • Protein or peptide: 30S ribosomal protein eS27
      • Protein or peptide: 30S ribosomal protein uS4
      • Protein or peptide: 30S ribosomal protein eS4
      • Protein or peptide: 30S ribosomal protein uS5
      • Protein or peptide: 30S ribosomal protein eS6
      • Protein or peptide: 30S ribosomal protein uS8
      • Protein or peptide: 30S ribosomal protein eS8
      • Protein or peptide: 30S ribosomal protein SXRibosome
      • Protein or peptide: 30S ribosomal protein eL41
    • Complex: 30S archaeal translation initiation complex
      • Protein or peptide: aIF1
      • Protein or peptide: aIF1A
      • Protein or peptide: aIF2-gamma
      • Protein or peptide: aIF2-beta
      • Protein or peptide: aIF2-alpha
    • Complex: 30S archaeal translation initiation complex
      • RNA: initiator Met-tRNA fMet from E. coli (A1U72 variant)
    • Complex: 30S archaeal translation initiation complex
      • RNA: mRNAMessenger RNA
  • Ligand: METHIONINE
  • Ligand: MAGNESIUM ION
  • Ligand: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
  • Ligand: ZINC ION

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Supramolecule #1: 30S archaeal translation initiation complex

SupramoleculeName: 30S archaeal translation initiation complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
Molecular weightExperimental: 982 KDa

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Supramolecule #2: 30S archaeal translation initiation complex

SupramoleculeName: 30S archaeal translation initiation complex / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#29
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)

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Supramolecule #3: 30S archaeal translation initiation complex

SupramoleculeName: 30S archaeal translation initiation complex / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #31, #33-#36
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #4: 30S archaeal translation initiation complex

SupramoleculeName: 30S archaeal translation initiation complex / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #32
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: synthetic construct (others)

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Supramolecule #5: 30S archaeal translation initiation complex

SupramoleculeName: 30S archaeal translation initiation complex / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #30
Source (natural)Organism: Pyrococcus (archaea)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #1: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 1
Details: 16S model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 492.800781 KDa
SequenceString: GUACUCCCUU UAAUUCCGGU UGAUCCUGCC GGAGGCCACU GCUAUGGGGG UCCGACUAAG CCAUGCGAGU CAAGGGGGCG UCCCUUCUG GGACGCCACC GGCGGACGGC UCAGUAACAC GUCGGUAACC UACCCUCGGG AGGGGGAUAA CCCCGGGAAA C UGGGGCUA ...String:
GUACUCCCUU UAAUUCCGGU UGAUCCUGCC GGAGGCCACU GCUAUGGGGG UCCGACUAAG CCAUGCGAGU CAAGGGGGCG UCCCUUCUG GGACGCCACC GGCGGACGGC UCAGUAACAC GUCGGUAACC UACCCUCGGG AGGGGGAUAA CCCCGGGAAA C UGGGGCUA AUCCCCCAUA GGCCUGGGGU ACUGGAAGGU CCCCAGGCCG AAAGGGAGCC GUAAGGCCCG CCCGAGGAUG CG GCGGCGG CCGAUUAGGU AGUUGGUGGG GUAACGGCCC ACCAAGCCGA AGAUCGGUAC GGGCCGUGAG AGCGGGAGCC CGG AGAUGG ACACUGAGAC ACGGGUCCAG GCCCUACGGG GCGCAGCAGG CGCGAAACCU CCGCAAUGCG GGAAACCGCG ACGG GGGGA CCCCCAGUGC CGUGCCUCUG GCACGGCUUU UCCGGAGUGU AAAAAGCUCC GGGAAUAAGG GCUGGGCAAG GCCGG UGGC AGCCGCCGCG GUAAUACCGG CGGCCCGAGU GGUGGCCACU AUUAUUGGGC CUAAAGCGGC CGUAGCCGGG CCCGUA AGU CCCUGGCGAA AUCCCACGGC UCAACCGUGG GGCUCGCUGG GGAUACUGCG GGCCUUGGGA CCGGGAGAGG CCGGGGG UA CCCCCGGGGU AGGGGUGAAA UCCUAUAAUC CCGGGGGGAC CGCCAGUGGC GAAGGCGCCC GGCUGGAACG GGUCCGAC G GUGAGCGCGG AAGGCCAGGG GAGCGAACCG GAUUAGAUAC CCGGGUAGUC CUGGCUGUAA AGGAUGCGGG CUAGGUGUC GGGCGAGCUU CGAGCUCGCC CGGUGCCGUA GGGAAGCCGU UAAGCCCGCC GCCUGGGGAG UACGGCCGCA AGGCUGAAAC UUAAAGGAA UUGGCGGGGG AGCACUACAA GGGGUGGAGC GUGCGGUUUA AUUGGAUUCA ACGCCGGGAA CCUCACCGGG G GCGACGGC AGGAUGAAGG CCAGGCUGAA GGUCUUGCCG GACGCGCCGA GAGGAGGUGC AUGGCCGCCG UCAGCUCGUA CC GUGAGGC GUCCACUUAA GUGUGGUAAC GAGCGAGACC CGCGCCCCCA GUUGCCAGUC CCUCCCGCUC GGGAGGGAGG CAC UCUGGG GGGACUGCCG GCGAUAAGCC GGAGGAAGGG GCGGGCGACG GUAGGUCAGU AUGCCCCGAA ACCCCCGGGC UACA CGCGC GCUACAAUGG GCGGGACAAU GGGACCCGAC CUCGAAAGGG GAAGGGAAUC CCCUAAACCC GCCCUCAGUU CGGAU CGCG GGCUGCAACU CGCCCGCGUG AAGCUGGAAU CCCUAGUACC CGCGUGUCAU CAUCGCGCGG CGAAUACGUC CCUGCU CCU UGCACACACC GCCCGUCACU CCACCCGAGC GGAGUCCGGG UGAGGCCUGA UCUCCUUCGG GAGGUCAGGU CGAGCCC GG GCUCCGUGAG GGGGGAGAAG UCGUAACAAG GUAACCGUAG GGGAACCUAC GGCUCGAUCA CCUCCUAUCG CCGGAA

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Macromolecule #30: mRNA

MacromoleculeName: mRNA / type: rna / ID: 30 / Number of copies: 1
Source (natural)Organism: Pyrococcus (archaea)
Molecular weightTheoretical: 8.394025 KDa
SequenceString:
AUUUGGAGGU GAUUUAAAUG CCAAAG

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Macromolecule #32: initiator Met-tRNA fMet from E. coli (A1U72 variant)

MacromoleculeName: initiator Met-tRNA fMet from E. coli (A1U72 variant) / type: rna / ID: 32
Details: tRNA model from Sulfolobus solfataricus (PDB code 3V11) used for fitting
Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.488629 KDa
SequenceString:
GCGGGGUGGA GCAGCCUGG(H2U) AGCUCGUCGG G(OMC)UCAUAACC CGAAGAUCGU CGG(5MU)UCAAAU CCGGCCCC C GCUACCA

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Macromolecule #2: 30S ribosomal protein uS3

MacromoleculeName: 30S ribosomal protein uS3 / type: protein_or_peptide / ID: 2
Details: 30S ribosomal protein uS3 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 23.464352 KDa
SequenceString: MAIERYFIRE AVREMLIDEF LEKELRRAGY GGLDIKKTPL GTKVIIFAAN PGYVIGRGGR RIRELTRILE KQFGLENPQI EVEEIKNPY LNAKVQAVRL AQALERGIHF RRAAYAALRA IMNNGARGVE IRLSGKLTGE RAKSIRFYQG YLAKVGNPAE T LVSKGYAQ ...String:
MAIERYFIRE AVREMLIDEF LEKELRRAGY GGLDIKKTPL GTKVIIFAAN PGYVIGRGGR RIRELTRILE KQFGLENPQI EVEEIKNPY LNAKVQAVRL AQALERGIHF RRAAYAALRA IMNNGARGVE IRLSGKLTGE RAKSIRFYQG YLAKVGNPAE T LVSKGYAQ ALLKLGVIGV KVAIMPPGAR LPDEIEIIEK PVEEEVVSNE AE

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Macromolecule #3: 50S ribosomal protein uL30

MacromoleculeName: 50S ribosomal protein uL30 / type: protein_or_peptide / ID: 3
Details: 50S ribosomal protein L7AE model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 13.414664 KDa
SequenceString:
MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL VIIAEDVDPE EIVAHLPPLC EEKEIPYIYV PSKKELGAA AGIEVAAASV AIIEPGKARD LVEEIAMKVK ELMK

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Macromolecule #4: 30S ribosomal protein uS10

MacromoleculeName: 30S ribosomal protein uS10 / type: protein_or_peptide / ID: 4
Details: 30S ribosomal protein uS10 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 11.769689 KDa
SequenceString:
MQKARIKIAS TNVRSLDEVA NQIKQIAERT GVRMSGPIPL PTKRIRITTR KSPDGEGSAT FDRWELRVHK RLIDIEADER AMRQIMRIR VPEDVTIEIE LIS

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Macromolecule #5: 30S ribosomal protein uS13

MacromoleculeName: 30S ribosomal protein uS13 / type: protein_or_peptide / ID: 5
Details: 30S ribosomal protein uS13 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 16.948904 KDa
SequenceString:
MANFRHIVRV AGVDLDGNKQ LRWALTAIKG IGINFATMVC RVAGLDPFMK AGYLTDEQVK KIEEILADPV AHGIPRWAVN RPKDYETGR DLHLITAKLD MAIREDIMRL RRIRAYRGIR HELGLPVRGQ RTRSNFRRGQ TVGVSRKKK

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Macromolecule #6: 30S ribosomal protein uS14

MacromoleculeName: 30S ribosomal protein uS14 / type: protein_or_peptide / ID: 6
Details: 30S ribosomal protein uS14 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 6.634046 KDa
SequenceString:
MAKADYNKRK PRKFGKGARR CIRCGQYGPI IRIQGLMLCR HCFREVAPKL GFRKYE

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Macromolecule #7: 30S ribosomal protein eS17

MacromoleculeName: 30S ribosomal protein eS17 / type: protein_or_peptide / ID: 7
Details: 30S ribosomal protein eS17 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 7.942465 KDa
SequenceString:
MGKIRQGFIK RVARELVNKY PNEFTTDFEH NKKKVQELTN VTSKKIRNRI AGYVTKLVRM KMEGKIL

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Macromolecule #8: 30S ribosomal protein uS19

MacromoleculeName: 30S ribosomal protein uS19 / type: protein_or_peptide / ID: 8
Details: 30S ribosomal protein uS19 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 15.357399 KDa
SequenceString:
MARKEFRYRG YTLEQLMNMS LEELARLFPA RQRRSLKRGL TPEQKKLLRK IRLAKKGKYK KPIRTHCRDM IILPEMVGLT IYVHNGKEF VPVEIKPEMI GHYLGEFAPT RKKVEHGAPG VGATRSSMFV AVK

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Macromolecule #9: 30S ribosomal protein eS19

MacromoleculeName: 30S ribosomal protein eS19 / type: protein_or_peptide / ID: 9
Details: 30S ribosomal protein eS19 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 17.394236 KDa
SequenceString:
MATVYDVPGD LLVERVAQRL KEIPEIKPPE WAPFVKTGRH KERLPEQEDW WYYRVASILR RVYLDGPVGI ERLRTYYGGR KNRGHAPEK FYKAGGSIIR KALQQLEAAG FVEKVPGKGR VITPKGRSFL DKIATELKKE LEEIIPELKK Y

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Macromolecule #10: 30S ribosomal protein eS28

MacromoleculeName: 30S ribosomal protein eS28 / type: protein_or_peptide / ID: 10
Details: 30S ribosomal protein eS28 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 8.116332 KDa
SequenceString:
MAEDEGYPAE VIEIIGRTGT TGDVTQVKVR ILEGRDKGRV IRRNVRGPVR IGDILILRET EREAREIKSR R

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Macromolecule #11: 30S ribosomal protein eS27

MacromoleculeName: 30S ribosomal protein eS27 / type: protein_or_peptide / ID: 11
Details: 30S ribosomal protein eS27 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 5.864958 KDa
SequenceString:
MGQKWKLYEI KDGKVIRKNK FCPRCGPGVF MADHGDRWAC GKCGYTEWKK

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Macromolecule #12: 30S ribosomal protein uS7

MacromoleculeName: 30S ribosomal protein uS7 / type: protein_or_peptide / ID: 12
Details: 30S ribosomal protein uS5 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 24.701791 KDa
SequenceString: MAKPLNERFF IPHEIKVMGR WSTEDVEVRD PSLKPYINLE PRLLPHTHGR HAKKHFGKAN VHIVERLINK IMRSGGSHYK VAGHFMRRE HRSLNSKKVK AYEVVKEAFK IIEQRTGKNP IQVLVWAIEN AAPREDTTSV MFGGIRYHVA VDISPMRRLD V ALRNIALG ...String:
MAKPLNERFF IPHEIKVMGR WSTEDVEVRD PSLKPYINLE PRLLPHTHGR HAKKHFGKAN VHIVERLINK IMRSGGSHYK VAGHFMRRE HRSLNSKKVK AYEVVKEAFK IIEQRTGKNP IQVLVWAIEN AAPREDTTSV MFGGIRYHVA VDISPMRRLD V ALRNIALG ASAKCYRNKM SFAEALAEEI ILAANKDPKS YAYSKKLEIE RIAESSR

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Macromolecule #13: 30S ribosomal protein uS9

MacromoleculeName: 30S ribosomal protein uS9 / type: protein_or_peptide / ID: 13
Details: 30S ribosomal protein uS9 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 15.317932 KDa
SequenceString:
MRIIQTTGKR KTAIARAVIR EGKGRVRING KPVEIIEPEI ARFTILEPLI LAGEEIWNSV DIDVKVEGGG FMGQAEAARM AIARALVEW TGDMSLKEKF MKYDRTMLVG DPRRTEPHKP NRSTKGPRAK RQKSYR

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Macromolecule #14: 30S ribosomal protein uS11

MacromoleculeName: 30S ribosomal protein uS11 / type: protein_or_peptide / ID: 14
Details: 30S ribosomal protein uS11 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 14.75695 KDa
SequenceString:
MSEEQVNIKK KEKWGIAHIY SSFNNTIIHI TDITGAETIS RWSGGMVVKA DRDEPSPYAA MLAARRAAEE ALEKGIVGVH IRVRAPGGS KSKTPGPGAQ AAIRALARAG LKIGRVEDVT PIPHDGTRPK GGRRGRRV

+
Macromolecule #15: 30S ribosomal protein uS12

MacromoleculeName: 30S ribosomal protein uS12 / type: protein_or_peptide / ID: 15
Details: 30S ribosomal protein uS12 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 16.477621 KDa
SequenceString:
MPGKKAPNGE FAGRKLKLKR KKFRWSDIRY KRRVLRLKEK SDPLEGAPQA RGIVLEKIAV EAKQPNSGMR KAVRVQLIKN GKVVTAFCP GDGAIKFIDE HDEVIIEGIG GPKGGSMGDI PGIRYKVVKV NRVSLKELVK GRKEKPRR

+
Macromolecule #16: 30S ribosomal protein uS15

MacromoleculeName: 30S ribosomal protein uS15 / type: protein_or_peptide / ID: 16
Details: 30S ribosomal protein uS15 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 18.640025 KDa
SequenceString:
MARMHARKRG KSGSKRPPRT APPIWVEYTV EEIENLVVKL RKEGYSTAMI GTILRDQYGI PSVKLFKDPD NPNRNLTITR ILEKHGLAP EIPEDLMFLI RRAVNLRKHL EQHPKDLHSM RGLQLIESKI RRLVKYYKRK GKLPKNWRYD PETAKLLVR

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Macromolecule #17: 30S ribosomal protein uS17

MacromoleculeName: 30S ribosomal protein uS17 / type: protein_or_peptide / ID: 17
Details: 30S ribosomal protein uS17 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 13.296521 KDa
SequenceString:
MMRDIGLRVQ PPAEKCDDPK CPWHGNLKIH GRVFEGIVVS DKPRKTVTVE RQYYFYLNKY ERYELRRSKI HAHNPPCINA KVGDKVLIA ETRPLSKTKH FVVVAVLERA EERR

+
Macromolecule #18: 30S ribosomal protein uS3

MacromoleculeName: 30S ribosomal protein uS3 / type: protein_or_peptide / ID: 18
Details: 30S ribosomal protein uS3 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 22.984107 KDa
SequenceString: MAAKRATTTR DKWKLKQWYI IYAPDFFGGV EVGLTPADDP EKVLNRVVEV TLKDVTGDFT KSHVKLYFQV YDVKGQNAYT KFKGMKLAR SYIRSLVRRK TTRIDGIFNI TTKDGYKLRV MAMAIAMRRI QTSQERAIRK IMQEIIYKKA EELNFKDFVL E SVNGKIAA ...String:
MAAKRATTTR DKWKLKQWYI IYAPDFFGGV EVGLTPADDP EKVLNRVVEV TLKDVTGDFT KSHVKLYFQV YDVKGQNAYT KFKGMKLAR SYIRSLVRRK TTRIDGIFNI TTKDGYKLRV MAMAIAMRRI QTSQERAIRK IMQEIIYKKA EELNFKDFVL E SVNGKIAA EIAKEAKKIY PLRKAEIRKI KVLEEPQVIA

+
Macromolecule #19: 30S ribosomal protein uS2

MacromoleculeName: 30S ribosomal protein uS2 / type: protein_or_peptide / ID: 19
Details: 30S ribosomal protein uS2 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 23.039861 KDa
SequenceString: MADEYLVPLD QYLAAGVHIG TQQKTKDMKK FIYRVRQDGL YVLDVRKTDE RLKVAGKFLA KFEPQSILAV SVRLYGQKPV KKFGEVTGA RAIPGRFLPG TMTNPAVKNF FEPDVLIVTD PRADHQAMRE AVEIGIPIVA LVDTENLLSY VDLAIPTNNK G RKALALIY ...String:
MADEYLVPLD QYLAAGVHIG TQQKTKDMKK FIYRVRQDGL YVLDVRKTDE RLKVAGKFLA KFEPQSILAV SVRLYGQKPV KKFGEVTGA RAIPGRFLPG TMTNPAVKNF FEPDVLIVTD PRADHQAMRE AVEIGIPIVA LVDTENLLSY VDLAIPTNNK G RKALALIY WILAREILYN RGEIQSREDF KIPVEEFEMK IVRR

+
Macromolecule #20: 30S ribosomal protein eS24

MacromoleculeName: 30S ribosomal protein eS24 / type: protein_or_peptide / ID: 20
Details: 30S ribosomal protein eS24 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 11.698564 KDa
SequenceString:
MEIRIKEIKE NKLIGRKEIY FEIYHPGEPT PSRKDVKGKL VAMLDLNPET TVIQYIRSYF GSYISKGYAK AYDSKERMLY IEPEYILIR DGLIEKKEGE

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Macromolecule #21: 30S ribosomal protein eS27

MacromoleculeName: 30S ribosomal protein eS27 / type: protein_or_peptide / ID: 21
Details: 30S ribosomal protein eS27 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 6.888388 KDa
SequenceString:
MAKPIIPMPR SRFLRVKCID CGNEQIVFSH PATKVRCLIC GATLVEPTGG KGIVKAKILE VLE

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Macromolecule #22: 30S ribosomal protein uS4

MacromoleculeName: 30S ribosomal protein uS4 / type: protein_or_peptide / ID: 22
Details: 30S ribosomal protein uS4 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 21.38191 KDa
SequenceString:
MGDPKRQRKK YETPPHPWIK ERLDRERVLM DKYELKNKKE LWKHETQLKN FRRRARRLLA ARGKQAEIER EQLLARLKRL GLLPEDAVL DDVLSLTIED ILERRLQTIV YKKGLARTMR QARQLIVHGH IEVNGQIIRS PSYLVLKEEE DTITYARTSP F ANPQHPER MMIEKAKQGG EA

+
Macromolecule #23: 30S ribosomal protein eS4

MacromoleculeName: 30S ribosomal protein eS4 / type: protein_or_peptide / ID: 23
Details: 30S ribosomal protein eS4 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 28.197023 KDa
SequenceString: MARKGPKRHL KRLAAPTSWY IERKAYKWAV RPRPGPHNMR TSIPLLYIVR DYLGYAKTAR EARKILNEGK FLVDGRVRKD YKFPVGIMD VVSIPETGEH YRVLPNRIGK LILHPISEDE AFIKPLRIRN KRMIKGARVQ LNFHDGTNHI VSIAEKDNYF T SYTVLMKV ...String:
MARKGPKRHL KRLAAPTSWY IERKAYKWAV RPRPGPHNMR TSIPLLYIVR DYLGYAKTAR EARKILNEGK FLVDGRVRKD YKFPVGIMD VVSIPETGEH YRVLPNRIGK LILHPISEDE AFIKPLRIRN KRMIKGARVQ LNFHDGTNHI VSIAEKDNYF T SYTVLMKV PEREILEVLP FEKGAYVFVT QGKNVARKGR IVEIKRFPMG WPDVVTIEDE EGELFDTLKE YAFVVGTDKP KI SLP

+
Macromolecule #24: 30S ribosomal protein uS5

MacromoleculeName: 30S ribosomal protein uS5 / type: protein_or_peptide / ID: 24
Details: 30S ribosomal protein uS5 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 26.590891 KDa
SequenceString: MSQEWKEYAK RVLDEWEPKT KLGMMVKEGQ ITDIHEIFRR GYQIKEPEII DVLLPEVNAR ENQEVLDIAL TVRMTDSGRR VRFRVLAAV GNRDGYVGLG IGHGKEVGIA IRKAINYAKL NIIEIKRGCG SWECRCRRPH SVPFAVEGKE GSVRVRLIPG P RGLGLVIG ...String:
MSQEWKEYAK RVLDEWEPKT KLGMMVKEGQ ITDIHEIFRR GYQIKEPEII DVLLPEVNAR ENQEVLDIAL TVRMTDSGRR VRFRVLAAV GNRDGYVGLG IGHGKEVGIA IRKAINYAKL NIIEIKRGCG SWECRCRRPH SVPFAVEGKE GSVRVRLIPG P RGLGLVIG DVGKKILRLA GVQDVWSQTF GETRTTVNFA KAVFNALYNT NRVAISPEMI ERYGIVVGRA MPTTFTLE

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Macromolecule #25: 30S ribosomal protein eS6

MacromoleculeName: 30S ribosomal protein eS6 / type: protein_or_peptide / ID: 25
Details: 30S ribosomal protein eS6 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 14.00233 KDa
SequenceString:
MATFKLVISD PKSGIAKQVE ITGAETEKLI GKRIGDQIPA KELNINLNEL FGKEFPEDVK LEIRGGTDKD GFPMRPDIHG PRRVRVLLS KGPGFRPKEK GERRKKTVRG NTISPEIVQV NVKLVY

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Macromolecule #26: 30S ribosomal protein uS8

MacromoleculeName: 30S ribosomal protein uS8 / type: protein_or_peptide / ID: 26
Details: 30S ribosomal protein uS8 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 14.684174 KDa
SequenceString:
MTLLDPLANA LSHITNSERV GKREVYIKPA SKLIGEVLRV MQKYGYIGEF EFIDDGRAGV YRVQLLGRIN KAGAIKPRFP VKVSEFEKW EKRFLPAFEF GILIVSTSQG VMSHKEAIEK GIGGRLIAYV Y

+
Macromolecule #27: 30S ribosomal protein eS8

MacromoleculeName: 30S ribosomal protein eS8 / type: protein_or_peptide / ID: 27
Details: 30S ribosomal protein eS8 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 14.293733 KDa
SequenceString:
MAIWQGRSLK KPSGGRIVLA RKKRKRELGR EPSNTRVAEQ DKRKIIRTYG GNRKVRLTAA AYANVFDKSG KGRKVRIIRV IENPANRQF ARRNIITKGA IIETEIGKAK VTSRPGQDGV VNAILLEE

+
Macromolecule #28: 30S ribosomal protein SX

MacromoleculeName: 30S ribosomal protein SX / type: protein_or_peptide / ID: 28
Details: 30S ribosomal protein SX model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 4.868993 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #29: 30S ribosomal protein eL41

MacromoleculeName: 30S ribosomal protein eL41 / type: protein_or_peptide / ID: 29
Details: 30S ribosomal protein eL41 model from Pyrococcus furiosus (PDB code 4V6U) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 3.050834 KDa
SequenceString:
MRWKWIKKRI RRLKRQRKKE RG

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Macromolecule #31: aIF1

MacromoleculeName: aIF1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 11.621787 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MPEICPRCGL PKELCVCEEI AKEEQKIKIY VTKRRFGKLM TIIEGFDTSV IDLKELAKKL KDICACGGTV KDNTIELQGD HRKKVAEEL VKMGFSRDSI EIR

+
Macromolecule #33: aIF1A

MacromoleculeName: aIF1A / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 13.078265 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MPKKERKVEG DEVIRVPLPE GNQLFGVVEQ ALGAGWMDVR CEDGKIRRCR IPGKLRRRVW IRVGDLVIVQ PWPVQSDKRG DIVYRYTQT QVDWLLRKGK ITQEFLTGGS LLVE

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Macromolecule #34: aIF2-gamma

MacromoleculeName: aIF2-gamma / type: protein_or_peptide / ID: 34
Details: aIF2-gamma model from Sulfolobus solfataricus (PDB code 3V11) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 45.84923 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAWPKVQPEV NIGVVGHVDH GKTTLVQAIT GIWTSKHSEE LKRGMTIKLG YAETNIGVCE SCKKPEAYVT EPSCKSCGSD DEPKFLRRI SFIDAPGHEV LMATMLSGAA LMDGAILVVA ANEPFPQPQT REHFVALGII GVKNLIIVQN KVDVVSKEEA L SQYRQIKQ ...String:
MAWPKVQPEV NIGVVGHVDH GKTTLVQAIT GIWTSKHSEE LKRGMTIKLG YAETNIGVCE SCKKPEAYVT EPSCKSCGSD DEPKFLRRI SFIDAPGHEV LMATMLSGAA LMDGAILVVA ANEPFPQPQT REHFVALGII GVKNLIIVQN KVDVVSKEEA L SQYRQIKQ FTKGTWAENV PIIPVSALHK INIDSLIEGI EEYIKTPYRD LSQKPVMLVI RSFDVNKPGT QFNELKGGVI GG SIIQGLF KVDQEIKVLP GLRVEKQGKV SYEPIFTKIS SIRFGDEEFK EAKPGGLVAI GTYLDPSLTK ADNLLGSIIT LAD AEVPVL WNIRIKYNLL ERVVGAKEML KVDPIRAKET LMLSVGSSTT LGIVTSVKKD EIEVELRRPV AVWSNNIRTV ISRQ IAGRW RMIGWGLVEI

+
Macromolecule #35: aIF2-beta

MacromoleculeName: aIF2-beta / type: protein_or_peptide / ID: 35
Details: aIF2-beta model from Sulfolobus solfataricus (PDB code 3V11) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 15.94274 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MSSEKEYVEM LDRLYSKLPE KGRKEGTQSL PNMIILNIGN TTIIRNFAEY CDRIRREDKI CMKYLLKELA APGNVDDKGE LVIQGKFSS QVINTLMERF LKAYVECSTC KSLDTILKKE KKSWYIVCLA CGAQTPVKPL

+
Macromolecule #36: aIF2-alpha

MacromoleculeName: aIF2-alpha / type: protein_or_peptide / ID: 36
Details: aIF2-alpha model from Sulfolobus solfataricus (PDB code 3V11) used for fitting
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pyrococcus abyssi GE5 (archaea)
Molecular weightTheoretical: 30.432355 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MIYSRSKLPS EGEILIATVK QVFDYGSYVS LDEYGGLQAF LPWSEVSSKW VKNIRDVLKE NRKVIVKVIR VDRRKGTVDV SLKKVTDDE RRKKNLQWKK IQRLDKILEL VSQKLKLSEK DAWEQVAWKL EAKYGDPITA IEKAVKEGEK ILIDAGVPEI W VKPLLEEA ...String:
MIYSRSKLPS EGEILIATVK QVFDYGSYVS LDEYGGLQAF LPWSEVSSKW VKNIRDVLKE NRKVIVKVIR VDRRKGTVDV SLKKVTDDE RRKKNLQWKK IQRLDKILEL VSQKLKLSEK DAWEQVAWKL EAKYGDPITA IEKAVKEGEK ILIDAGVPEI W VKPLLEEA SKHAEERKVK MSGLITVRTN EPLGVEKIKE VISKALENIE QDYESLLNIK IYTIGAPRYR VDVVGTNPKE AS EALNQII SNLIKIGKEE NVDISVVKK

+
Macromolecule #37: METHIONINE

MacromoleculeName: METHIONINE / type: ligand / ID: 37 / Number of copies: 1 / Formula: MET
Molecular weightTheoretical: 149.211 Da
Chemical component information

ChemComp-MET:
METHIONINE / Methionine

+
Macromolecule #38: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 38 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #39: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER

MacromoleculeName: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / type: ligand / ID: 39 / Number of copies: 1 / Formula: GNP
Molecular weightTheoretical: 522.196 Da
Chemical component information

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

+
Macromolecule #40: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 40 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 44.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING
Final reconstructionResolution.type: BY AUTHOR / Resolution: 5.34 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 12600
FSC plot (resolution estimation)

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