EMDB-0955: Cryo-EM structure of 90S small subunit preribosomes in transition...

Basic information

• Entry: Database: EMDB / ID: EMD-0955
• Title: Cryo-EM structure of 90S small subunit preribosomes in transition states (State C1)

Sample

• Complex: 90S pre-ribosome (Dhr1-depleted, state C1)
  • RNA: x 3 types
  • Protein or peptide: x 51 types
• Ligand: x 3 types

• Keywords: ribosome assembly / 90S to pre-40S transition / cryo-EM / Dhr1 / RIBOSOME

Function / homology

Function:

rRNA cytidine N-acetyltransferase activity / Noc4p-Nop14p complex / rRNA acetylation involved in maturation of SSU-rRNA / tRNA N-acetyltransferase activity / t-UTP complex / rRNA small subunit pseudouridine methyltransferase Nep1 / tRNA acetylation / RNA fragment catabolic process / rRNA 2'-O-methylation / Pwp2p-containing subcomplex of 90S preribosome / histone H2AQ104 methyltransferase activity / nuclear microtubule / Mpp10 complex / snoRNA guided rRNA 2'-O-methylation / rRNA (pseudouridine) methyltransferase activity / rRNA modification / regulation of rRNA processing / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / septum digestion after cytokinesis / tRNA re-export from nucleus / snRNA binding / box C/D sno(s)RNA 3'-end processing / regulation of transcription by RNA polymerase I / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA methyltransferase activity / rDNA heterochromatin / positive regulation of rRNA processing / single-stranded telomeric DNA binding / box C/D methylation guide snoRNP complex / tRNA export from nucleus / U4/U6 snRNP / rRNA base methylation / rRNA primary transcript binding / SUMOylation of RNA binding proteins / sno(s)RNA-containing ribonucleoprotein complex / small nuclear ribonucleoprotein complex / protein localization to nucleolus / U4 snRNA binding / mTORC1-mediated signalling / O-methyltransferase activity / Protein hydroxylation / rRNA methylation / U3 snoRNA binding / mRNA modification / U4 snRNP / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / poly(A)+ mRNA export from nucleus / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / preribosome, small subunit precursor / Ribosomal scanning and start codon recognition / snoRNA binding / precatalytic spliceosome / establishment of cell polarity / positive regulation of transcription by RNA polymerase I / Major pathway of rRNA processing in the nucleolus and cytosol / spliceosomal complex assembly / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / 90S preribosome / regulation of translational fidelity / ribosomal subunit export from nucleus / RNA processing / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / vesicle-mediated transport / U4/U6 x U5 tri-snRNP complex / enzyme activator activity / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / RNA endonuclease activity / nuclear periphery / Transferases; Transferring one-carbon groups; Methyltransferases / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / translational initiation / spliceosomal complex / maintenance of translational fidelity / mRNA splicing, via spliceosome / rRNA processing / ribosomal small subunit biogenesis / protein transport / peroxisome / ribosomal small subunit assembly / cytosolic small ribosomal subunit / cytoplasmic translation / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / GTPase activity / mRNA binding / GTP binding / nucleolus / ATP hydrolysis activity

Domain/homology:

Apoptosis-antagonizing transcription factor, C-terminal / AATF leucine zipper-containing domain / Protein AATF/Bfr2 / Apoptosis-antagonizing transcription factor, C-terminal / Apoptosis antagonizing transcription factor / NUC153 / Nucleolar protein 10/Enp2 / NUC153 domain / U3 small nucleolar RNA-associated protein 8 / : / Utp8, N-terminal beta propeller / Utp8, C-terminal / U3 small nucleolar RNA-associated protein 20, N-terminal / U3 snoRNA associated / Nucleolar complex protein 4 / U3 small nucleolar RNA-associated protein 20, C-terminal / : / U3 small nucleolar RNA-associated protein 20, N-terminal / U3 snoRNA associated / U3 small nucleolar RNA-associated protein 20, C-terminal / Possible tRNA binding domain / RNA cytidine acetyltransferase NAT10 / Possible tRNA binding domain / Helicase domain / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / TmcA/NAT10/Kre33 / Helicase / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / GNAT acetyltransferase 2 / Nucleolar protein 14 / Nop14-like family / U3 small nucleolar RNA-associated protein 6 / U3 small nucleolar RNA-associated protein 6 / Small-subunit processome, Utp11 / Sof1-like protein / BING4, C-terminal domain / Fcf2 pre-rRNA processing, C-terminal / rRNA-processing protein Fcf1, PIN domain / Fcf2/DNTTIP2 / WD repeat-containing protein WDR46/Utp7 / : / : / Utp11 protein / Sof1-like domain / BING4CT (NUC141) domain / Fcf2 pre-rRNA processing / BING4CT (NUC141) domain / rRNA-processing protein Fcf1/Utp23 / : / Fcf1 / Small-subunit processome, Utp14 / U3 small nucleolar RNA-associated protein 15, C-terminal / Utp14 protein / UTP15 C terminal / Sas10 C-terminal domain / Sas10 C-terminal domain / Nucleolar protein 58/56, N-terminal / U3 small nucleolar RNA-associated protein 18 / NOP5NT (NUC127) domain / : / BP28, C-terminal domain / RNA 3'-terminal phosphate cyclase-like, conserved site / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosome biogenesis protein Bms1, N-terminal / U3 small nucleolar RNA-associated protein 10 / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 10 / RNA 3'-terminal phosphate cyclase signature. / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 13, C-terminal / Periodic tryptophan protein 2 / Utp13 specific WD40 associated domain / Small-subunit processome, Utp12 / Dip2/Utp12 Family / Ribosomal RNA-processing protein Rrp9-like / Sas10/Utp3/C1D / Small-subunit processome, Utp21 / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / RNA 3'-terminal phosphate cyclase type 2 / Sas10/Utp3/C1D family / Mpp10 protein / Utp21 specific WD40 associated putative domain / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / EMG1/NEP1 methyltransferase / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase, insert domain / RNA 3'-terminal phosphate cyclase domain / RNA 3'-terminal phosphate cyclase, insert domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase (RTC), insert domain / Nucleolar protein Nop56/Nop58 / CCAAT-binding factor / CBF/Mak21 family / Bystin / Bystin / rRNA 2'-O-methyltransferase fibrillarin-like / Fibrillarin, conserved site / Fibrillarin / Fibrillarin signature.

Component:

Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / rRNA 2'-O-methyltransferase fibrillarin / Periodic tryptophan protein 2 / Small ribosomal subunit protein uS7 / U3 small nucleolar ribonucleoprotein protein IMP3 / Protein SOF1 / U3 small nucleolar RNA-associated protein 11 / U3 small nucleolar RNA-associated protein 20 / Essential nuclear protein 1 / U3 small nucleolar RNA-associated protein 9 / 13 kDa ribonucleoprotein-associated protein / U3 small nucleolar RNA-associated protein 7 / U3 small nucleolar ribonucleoprotein protein LCP5 / U3 small nucleolar RNA-associated protein 18 / U3 small nucleolar RNA-associated protein 10 / U3 small nucleolar RNA-associated protein MPP10 / Ribosome biogenesis protein ENP2 / U3 small nucleolar RNA-associated protein 8 / RNA cytidine acetyltransferase / U3 small nucleolar ribonucleoprotein protein IMP4 / U3 small nucleolar RNA-associated protein 6 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 15 / U3 small nucleolar RNA-associated protein 14 / rRNA-processing protein FCF1 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / Ribosomal RNA-processing protein 9 / Nucleolar complex protein 4 / Protein BFR2 / U3 small nucleolar RNA-associated protein 4 / RNA 3'-terminal phosphate cyclase-like protein / U3 small nucleolar RNA-associated protein 16 / Ribosome biogenesis protein BMS1 / rRNA-processing protein FCF2 / Something about silencing protein 10 / U3 small nucleolar RNA-associated protein 12 / Nucleolar protein 56 / Nucleolar protein 58 / Small ribosomal subunit protein eS28A / Nucleolar complex protein 14

• Biological species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) / Saccharomyces cerevisiae S288C (yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 4.8 Å
• Authors: Du Y / Ye K

Funding support

China, 2 items

Organization	Grant number	Country
National Natural Science Foundation of China (NSFC)	91940302, 91540201, 31430024, 31325007	China
Chinese Academy of Sciences	XDB08010203	China

• Citation: Journal: Science / Year: 2020; Title: Cryo-EM structure of 90 small ribosomal subunit precursors in transition states.; Authors: Yifei Du / Weidong An / Xing Zhu / Qi Sun / Jia Qi / Keqiong Ye / ; Abstract: The 90 preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40 ribosome. Here, we gained insight into this transition by determining cryo-electron microscopy structures of intermediates in the path from the 90 to the pre-40 The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5' external transcribed spacer (5' ETS) is degraded within 90, driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90 through helicase Mtr4 and is primed to digest the 3' end of the 5' ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5' ETS degradation in 90 progression.

History

Deposition	Jan 14, 2020	-
Header (metadata) release	Sep 16, 2020	-
Map release	Sep 16, 2020	-
Update	Mar 27, 2024	-
Current status	Mar 27, 2024	Processing site: PDBj / Status: Released

Map

• File: Download / File: emd_0955.map.gz / Format: CCP4 / Size: 343 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.334 Å

Density

Contour Level	By AUTHOR: 0.022 / Movie #1: 0.022
Minimum - Maximum	-0.04739722 - 0.09379589
Average (Standard dev.)	0.00042653875 (±0.0032622444)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 448 448 448 Spacing 448 448 448
Cell	A=B=C: 597.632 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.334	1.334	1.334
M x/y/z	448	448	448
origin x/y/z	0.000	0.000	0.000
length x/y/z	597.632	597.632	597.632
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	448	448	448
D min/max/mean	-0.047	0.094	0.000

Supplemental data

Sample components

Entire : 90S pre-ribosome (Dhr1-depleted, state C1)

• Entire: Name: 90S pre-ribosome (Dhr1-depleted, state C1)

Components

• Complex: 90S pre-ribosome (Dhr1-depleted, state C1)
  • RNA: U3 snoRNA
  • RNA: 5' ETS
  • RNA: 18S pre-rRNA
  • Protein or peptide: 40S ribosomal protein S4-A
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S6-A
  • Protein or peptide: 40S ribosomal protein S8-A
  • Protein or peptide: 40S ribosomal protein S9-A
  • Protein or peptide: 40S ribosomal protein S11-A
  • Protein or peptide: 40S ribosomal protein S16-A
  • Protein or peptide: 40S ribosomal protein S23-A
  • Protein or peptide: 40S ribosomal protein S24-A
  • Protein or peptide: 40S ribosomal protein S28-A
  • Protein or peptide: rRNA 2'-O-methyltransferase fibrillarin
  • Protein or peptide: Nucleolar protein 56
  • Protein or peptide: Nucleolar protein 58
  • Protein or peptide: Ribosomal RNA-processing protein 9
  • Protein or peptide: 13 kDa ribonucleoprotein-associated protein
  • Protein or peptide: U3 small nucleolar RNA-associated protein 4
  • Protein or peptide: U3 small nucleolar RNA-associated protein 5
  • Protein or peptide: U3 small nucleolar RNA-associated protein 8
  • Protein or peptide: U3 small nucleolar RNA-associated protein 9
  • Protein or peptide: U3 small nucleolar RNA-associated protein 10
  • Protein or peptide: U3 small nucleolar RNA-associated protein 15
  • Protein or peptide: NET1-associated nuclear protein 1
  • Protein or peptide: Periodic tryptophan protein 2
  • Protein or peptide: U3 small nucleolar RNA-associated protein 12
  • Protein or peptide: U3 small nucleolar RNA-associated protein 13
  • Protein or peptide: U3 small nucleolar RNA-associated protein 18
  • Protein or peptide: U3 small nucleolar RNA-associated protein 21
  • Protein or peptide: U3 small nucleolar RNA-associated protein 6
  • Protein or peptide: Bud site selection protein 21
  • Protein or peptide: U3 small nucleolar RNA-associated protein 7
  • Protein or peptide: U3 small nucleolar RNA-associated protein 11
  • Protein or peptide: U3 small nucleolar RNA-associated protein MPP10
  • Protein or peptide: U3 small nucleolar ribonucleoprotein protein IMP3
  • Protein or peptide: U3 small nucleolar ribonucleoprotein protein IMP4
  • Protein or peptide: Something about silencing protein 10
  • Protein or peptide: Protein SOF1
  • Protein or peptide: rRNA-processing protein FCF2
  • Protein or peptide: rRNA-processing protein FCF1
  • Protein or peptide: Ribosome biogenesis protein ENP2
  • Protein or peptide: U3 small nucleolar ribonucleoprotein protein LCP5
  • Protein or peptide: Ribosomal RNA small subunit methyltransferase NEP1
  • Protein or peptide: Ribosome biogenesis protein BMS1
  • Protein or peptide: RNA 3'-terminal phosphate cyclase-like protein
  • Protein or peptide: RNA cytidine acetyltransferase
  • Protein or peptide: Nucleolar complex protein 14
  • Protein or peptide: Nucleolar complex protein 4
  • Protein or peptide: U3 small nucleolar RNA-associated protein 20
  • Protein or peptide: U3 small nucleolar RNA-associated protein 14
  • Protein or peptide: Essential nuclear protein 1
  • Protein or peptide: Protein BFR2
  • Protein or peptide: Unassigned helices
• Ligand: ZINC ION
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION

Supramolecule #1: 90S pre-ribosome (Dhr1-depleted, state C1)

• Supramolecule: Name: 90S pre-ribosome (Dhr1-depleted, state C1) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#54
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 5 MDa

Macromolecule #1: U3 snoRNA

• Macromolecule: Name: U3 snoRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 106.503258 KDa

Sequence

String:

GUCGACGUAC UUCAUAGGAU CAUUUCUAUA GGAAUCGUCA CUCUUUGACU CUUCAAAAGA GCCACUGAAU CCAACUUGGU UGAUGAGUC CCAUAACCUU UGUACCCCAG AGUGAGAAAC CGAAAUUGAA UCUAAAUUAG CUUGGUCCGC AAUCCUUAGC G GUUCGGCC AUCUAUAAUU UUGAAUAAAA AUUUUGCUUU GCCGUUGCAU UUGUAGUUUU UUCCUUUGGA AGUAAUUACA AU AUUUUAU GGCGCGAUGA UCUUGACCCA UCCUAUGUAC UUCUUUUUUG AAGGGAUAGG GCUCUAUGGG UGGGUACAAA UGG CAGUCU GACAAGU

Macromolecule #2: 5' ETS

• Macromolecule: Name: 5' ETS / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 225.543094 KDa

Sequence

String:

AUGCGAAAGC AGUUGAAGAC AAGUUCGAAA AGAGUUUGGA AACGAAUUCG AGUAGGCUUG UCGUUCGUUA UGUUUUUGUA AAUGGCCUC GUCAAACGGU GGAGAGAGUC GCUAGGUGAU CGUCAGAUCU GCCUAGUCUC UAUACAGCGU GUUUAAUUGA C AUGGGUUG AUGCGUAUUG AGAGAUACAA UUUGGGAAGA AAUUCCCAGA GUGUGUUUCU UUUGCGUUUA ACCUGAACAG UC UCAUCGU GGGCAUCUUG CGAUUCCAUU GGUGAGCAGC GAAGGAUUUG GUGGAUUACU AGCUAAUAGC AAUCUAUUUC AAA GAAUUC AAACUUGGGG GAAUGCCUUG UUGAAUAGCC GGUCGCAAGA CUGUGAUUCU UCAAGUGUAA CCUCCUCUCA AAUC AGCGA UAUCAAACGU ACCAUUCCGU GAAACACCGG GGUAUCUGUU UGGUGGAACC UGAUUAGAGG AAACUCAAAG AGUGC UAUG GUAUGGUGAC GGAGUGCGCU GGUCAAGAGU GUAAAAGCUU UUUGAACAGA GAGCAUUUCC GGCAGCAGAG AGACCU GAA AAAGCAAUUU UUCUGGAAUU UCAGCUGUUU CCAAACUCAA UAAGUAUCUU CUAGCAAGAG GGAAUAGGUG GGAAAAA AA AAAAGAGAUU UCGGUUUCUU UCUUUUUUAC UGCUUGUUGC UUCUUCUUUU AAGAUAGU

Macromolecule #3: 18S pre-rRNA

• Macromolecule: Name: 18S pre-rRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 582.381688 KDa

Sequence

String:

AAGAUAGUUA UCUGGUUGAU CCUGCCAGUA GUCAUAUGCU UGUCUCAAAG AUUAAGCCAU GCAUGUCUAA GUAUAAGCAA UUUAUACAG UGAAACUGCG AAUGGCUCAU UAAAUCAGUU AUCGUUUAUU UGAUAGUUCC UUUACUACAU GGUAUAACUG U GGUAAUUC UAGAGCUAAU ACAUGCUUAA AAUCUCGACC CUUUGGAAGA GAUGUAUUUA UUAGAUAAAA AAUCAAUGUC UU CGGACUC UUUGAUGAUU CAUAAUAACU UUUCGAAUCG CAUGGCCUUG UGCUGGCGAU GGUUCAUUCA AAUUUCUGCC CUA UCAACU UUCGAUGGUA GGAUAGUGGC CUACCAUGGU UUCAACGGGU AACGGGGAAU AAGGGUUCGA UUCCGGAGAG GGAG CCUGA GAAACGGCUA CCACAUCCAA GGAAGGCAGC AGGCGCGCAA AUUACCCAAU CCUAAUUCAG GGAGGUAGUG ACAAU AAAU AACGAUACAG GGCCCAUUCG GGUCUUGUAA UUGGAAUGAG UACAAUGUAA AUACCUUAAC GAGGAACAAU UGGAGG GCA AGUCUGGUGC CAGCAGCCGC GGUAAUUCCA GCUCCAAUAG CGUAUAUUAA AGUUGUUGCA GUUAAAAAGC UCGUAGU UG AACUUUGGGC CCGGUUGGCC GGUCCGAUUU UUUCGUGUAC UGGAUUUCCA ACGGGGCCUU UCCUUCUGGC UAACCUUG A GUCCUUGUGG CUCUUGGCGA ACCAGGACUU UUACUUUGAA AAAAUUAGAG UGUUCAAAGC AGGCGUAUUG CUCGAAUAU AUUAGCAUGG AAUAAUAGAA UAGGACGUUU GGUUCUAUUU UGUUGGUUUC UAGGACCAUC GUAAUGAUUA AUAGGGACGG UCGGGGGCA UCAGUAUUCA AUUGUCAGAG GUGAAAUUCU UGGAUUUAUU GAAGACUAAC UACUGCGAAA GCAUUUGCCA A GGACGUUU UCAUUAAUCA AGAACGAAAG UUAGGGGAUC GAAGAUGAUC AGAUACCGUC GUAGUCUUAA CCAUAAACUA UG CCGACUA GGGAUCGGGU GGUGUUUUUU UAAUGACCCA CUCGGCACCU UACGAGAAAU CAAAGUCUUU GGGUUCUGGG GGG AGUAUG GUCGCAAGGC UGAAACUUAA AGGAAUUGAC GGAAGGGCAC CACCAGGAGU GGAGCCUGCG GCUUAAUUUG ACUC AACAC GGGGAAACUC ACCAGGUCCA GACACAAUAA GGAUUGACAG AUUGAGAGCU CUUUCUUGAU UUUGUGGGUG GUGGU GCAU GGCCGUUCUU AGUUGGUGGA GUGAUUUGUC UGCUUAAUUG CGAUAACGAA CGAGACCUUA ACCUACUAAA UAGUGG UGC UAGCAUUUGC UGGUUAUCCA CUUCUUAGAG GGACUAUCGG UUUCAAGCCG AUGGAAGUUU GAGGCAAUAA CAGGUCU GU GAUGCCCUUA GACGUUCUGG GCCGCACGCG CGCUACACUG ACGGAGCCAG CGAGUCUAAC CUUGGCCGAG AGGUCUUG G UAAUCUUGUG AAACUCCGUC GUGCUGGGGA UAGAGCAUUG UAAUUAUUGC UCUUCAACGA GGAAUUCCUA GUAAGCGCA AGUCAUCAGC UUGCGUUGAU UACGUCCCUG CCCUUUGUAC ACACCGCCCG UCGCUAGUAC CGAUUGAAUG GCUUAGUGAG GCCUCAGGA UCUGCUUAGA GAAGGGGGCA ACUCCAUCUC AGAGCGGAGA AUUUGGACAA ACUUGGUCAU UUAGAGGAAC U AAAAGUCG UAACAAGGUU UCCGUAGGUG AACCUGCGGA AGGAUCAUUA

Macromolecule #4: 40S ribosomal protein S4-A

• Macromolecule: Name: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 29.46933 KDa

Sequence

String:

MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ITDFIKFDAG KLVYVTGGRN LGRIGTIVHK ERHDGGFDLV HIKDSLDNTF VTRLNNVFVI GEQGKPYISL PK GKGIKLS IAEERDRRRA QQGL

UniProtKB: Small ribosomal subunit protein eS4A

Macromolecule #5: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.0726 KDa

Sequence

String:

MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV NQAIALLTIG AREAAFRNIK TIAETLAEEL INAAKGSSTS YAIKKKDELE RVAKSNR

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #6: 40S ribosomal protein S6-A

• Macromolecule: Name: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.054486 KDa

Sequence

String:

MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK RRASSLKA

UniProtKB: Small ribosomal subunit protein eS6A

Macromolecule #7: 40S ribosomal protein S8-A

• Macromolecule: Name: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.537803 KDa

Sequence

String:

MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

UniProtKB: Small ribosomal subunit protein eS8A

Macromolecule #8: 40S ribosomal protein S9-A

• Macromolecule: Name: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 22.487893 KDa

Sequence

String:

MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR PGRVARRNAA RKAEASGEAA DEADEADEE

UniProtKB: Small ribosomal subunit protein uS4A

Macromolecule #9: 40S ribosomal protein S11-A

• Macromolecule: Name: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 17.785934 KDa

Sequence

String:

MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTKM HRTIVIRRA YLHYIPKYNR YEKRHKNVPV HVSPAFRVQV GDIVTVGQCR PISKTVRFNV VKVSAAAGKA NKQFAKF

UniProtKB: Small ribosomal subunit protein uS17A

Macromolecule #10: 40S ribosomal protein S16-A

• Macromolecule: Name: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.87749 KDa

Sequence

String:

MSAVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVE PEILRFKVYE PLLLVGLDKF SNIDIRVRVT GGGHVSQVYA IRQAIAKGL VAYHQKYVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGKGARSRFQ KSYR

UniProtKB: Small ribosomal subunit protein uS9A

Macromolecule #11: 40S ribosomal protein S23-A

• Macromolecule: Name: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 16.073896 KDa

Sequence

String:

MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK GIVLEKLGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

UniProtKB: Small ribosomal subunit protein uS12A

Macromolecule #12: 40S ribosomal protein S24-A

• Macromolecule: Name: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 15.362848 KDa

Sequence

String:

MSDAVTIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEVYKA EKDAVSVFGF RTQFGGGKSV GFGLVYNSVA EAKKFEPTY RLVRYGLAEK VEKASRQQRK QKKNRDKKIF GTGKRLAKKV ARRNAD

UniProtKB: Small ribosomal subunit protein eS24A

Macromolecule #13: 40S ribosomal protein S28-A

• Macromolecule: Name: 40S ribosomal protein S28-A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 7.605847 KDa

Sequence

String:

MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE NDILVLMESE REARRLR

UniProtKB: Small ribosomal subunit protein eS28A

Macromolecule #14: rRNA 2'-O-methyltransferase fibrillarin

• Macromolecule: Name: rRNA 2'-O-methyltransferase fibrillarin / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO; EC number: Transferases; Transferring one-carbon groups; Methyltransferases
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 34.525418 KDa

Sequence

String:

MSFRPGSRGG SRGGSRGGFG GRGGSRGGAR GGSRGGFGGR GGSRGGARGG SRGGFGGRGG SRGGARGGSR GGRGGAAGGA RGGAKVVIE PHRHAGVYIA RGKEDLLVTK NMAPGESVYG EKRISVEEPS KEDGVPPTKV EYRVWNPFRS KLAAGIMGGL D ELFIAPGK KVLYLGAASG TSVSHVSDVV GPEGVVYAVE FSHRPGRELI SMAKKRPNII PIIEDARHPQ KYRMLIGMVD CV FADVAQP DQARIIALNS HMFLKDQGGV VISIKANCID STVDAETVFA REVQKLREER IKPLEQLTLE PYERDHCIVV GRY MRSGLK K

UniProtKB: rRNA 2'-O-methyltransferase fibrillarin

Macromolecule #15: Nucleolar protein 56

• Macromolecule: Name: Nucleolar protein 56 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 56.961152 KDa

Sequence

String:

MAPIEYLLFE EPTGYAVFKV KLQQDDIGSR LKEVQEQIND FGAFTKLIEL VSFAPFKGAA EALENANDIS EGLVSESLKA ILDLNLPKA SSKKKNITLA ISDKNLGPSI KEEFPYVDCI SNELAQDLIR GVRLHGEKLF KGLQSGDLER AQLGLGHAYS R AKVKFSVQ KNDNHIIQAI ALLDQLDKDI NTFAMRVKEW YGWHFPELAK LVPDNYTFAK LVLFIKDKAS LNDDSLHDLA AL LNEDSGI AQRVIDNARI SMGQDISETD MENVCVFAQR VASLADYRRQ LYDYLCEKMH TVAPNLSELI GEVIGARLIS HAG SLTNLS KQAASTVQIL GAEKALFRAL KTKGNTPKYG LIYHSGFISK ASAKNKGRIS RYLANKCSMA SRIDNYSEEP SNVF GSVLK KQVEQRLEFY NTGKPTLKNE LAIQEAMELY NKDKPAAEVE ETKEKESSKK RKLEDDDEEK KEKKEKKSKK EKKEK KEKK DKKEKKDKKE KKDKKKKSKD

UniProtKB: Nucleolar protein 56

Macromolecule #16: Nucleolar protein 58

• Macromolecule: Name: Nucleolar protein 58 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 57.060344 KDa

Sequence

String:

MAYVLTETSA GYALLKASDK KIYKSSSLIQ DLDSSDKVLK EFKIAAFSKF NSAANALEEA NSIIEGKVSS QLEKLLEEIK KDKKSTLIV SETKLANAIN KLGLNFNVVS DAVTLDIYRA IKEYLPELLP GMSDNDLSKM SLGLAHSIGR HKLKFSADKV D VMIIQAIA LLDDLDKELN TYAMRCKEWY GWHFPELAKI VTDSVAYARI ILTMGIRSKA SETDLSEILP EEIEERVKTA AE VSMGTEI TQTDLDNINA LAEQIVEFAA YREQLSNYLS ARMKAIAPNL TQLVGELVGA RLIAHSGSLI SLAKSPASTI QIL GAEKAL FRALKTKHDT PKYGLLYHAS LVGQATGKNK GKIARVLAAK AAVSLRYDAL AEDRDDSGDI GLESRAKVEN RLSQ LEGRD LRTTPKVVRE AKKVEMTEAR AYNADADTAK AASDSESDSD DEEEEKKEKK EKKRKRDDDE DSKDSKKAKK EKKDK KEKK EKKEKKEKKE KKEKKEKKSK KEKKEKK

UniProtKB: Nucleolar protein 58

Macromolecule #17: Ribosomal RNA-processing protein 9

• Macromolecule: Name: Ribosomal RNA-processing protein 9 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 65.146969 KDa

Sequence

String:

MSDVTQQKKR KRSKGEVNPS KPTVDEEITD PSSNEDEQLE VSDEEDALES EEEFEGENPA DKRRRLAKQY LENLKSEAND ILTDNRNAE EKDLNNLKER TIDEYNNFDA GDLDKDIIAS RLKEDVAEQQ GRVFRYFGDK LLISEAKQSF TRVGENNLTC I SCFQPVLN KYTFEESSNG DKNKGRLFAY TVSKDLQLTK YDITDFSKRP KKLKYAKGGA KYIPTSKHEY ENTTEGHYDE IL TVAASPD GKYVVTGGRD RKLIVWSTES LSPVKVIPTK DRRGEVLSLA FRKNSDQLYA SCADFKIRTY SINQFSQLEI LYG HHDIVE DISALAMERC VTVGARDRTA MLWKIPDETR LTFRGGDEPQ KLLRRWMKEN AKEGEDGEVK YPDESEAPLF FCEG SIDVV SMVDDFHFIT GSDNGNICLW SLAKKKPIFT ERIAHGILPE PSFNDISGET DEELRKRQLQ GKKLLQPFWI TSLYA IPYS NVFISGSWSG SLKVWKISDN LRSFELLGEL SGAKGVVTKI QVVESGKHGK EKFRILASIA KEHRLGRWIA NVSGAR NGI YSAVIDQTGF

UniProtKB: Ribosomal RNA-processing protein 9

Macromolecule #18: 13 kDa ribonucleoprotein-associated protein

• Macromolecule: Name: 13 kDa ribonucleoprotein-associated protein / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 13.582855 KDa

Sequence

String:

MSAPNPKAFP LADAALTQQI LDVVQQAANL RQLKKGANEA TKTLNRGISE FIIMAADCEP IEILLHLPLL CEDKNVPYVF VPSRVALGR ACGVSRPVIA ASITTNDASA IKTQIYAVKD KIETLLI

UniProtKB: 13 kDa ribonucleoprotein-associated protein

Macromolecule #19: U3 small nucleolar RNA-associated protein 4

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 4 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 87.909242 KDa

Sequence

String:

MSSSLLSVLK EKSRSLKIRN KPVKMTSQER MIVHRCRFVD FTPATITSLA FSHKSNINKL TPSDLRLAIG RSNGNIEIWN PRNNWFQEM VIEGGKDRSI EGLCWSNVNG ESLRLFSIGG STVVTEWDLA TGLPLRNYDC NSGVIWSISI NDSQDKLSVG C DNGTVVLI DISGGPGVLE HDTILMRQEA RVLTLAWKKD DFVIGGCSDG RIRIWSAQKN DENMGRLLHT MKVDKAKKES TL VWSVIYL PRTDQIASGD STGSIKFWDF QFATLNQSFK AHDADVLCLT TDTDNNYVFS AGVDRKIFQF SQNTNKSQKN NRW VNSSNR LLHGNDIRAI CAYQSKGADF LVSGGVEKTL VINSLTSFSN GNYRKMPTVE PYSKNVLVNK EQRLVVSWSE STVK IWTMG TDSSTEQNYK LVCKLTLKDD QNISTCSLSP DGQVLVVGRP STTKVFHLQP VGNKLKVTKL DNDLLLRTST KLVKF IDNS KIVICSCEDD VFIVDLESEE DEKPQEVELL EVTSTKSSIK VPYINRINHL EVDQNIAVIS RGCGVVDILD LKARIS KPL ARLNNFITAV HINTSRKSVV VITADNKIYE FNMNLNSEAE NEDSESVLTQ WSKNNTDNLP KEWKTLKENC VGIFSDI EN SSRLWFWGAT WISRIDFDVD FPINKRRKQK KRTHEGLTIT DESNFMNDEE DDEDDDIDME ISENLNVLLN QGNKIKST D VQRNEESSGH FFFTDKYKPL LFVDLISSNE LAIIERNPLT FHSKQKAFIQ PKLVF

UniProtKB: U3 small nucleolar RNA-associated protein 4

Macromolecule #20: U3 small nucleolar RNA-associated protein 5

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 5 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 72.079445 KDa

Sequence

String:

MDSPVLQSAY DPSGQYLCYV TVALDKQRVG VQPTQRATSS GVDTVWNENF LYLEDSKLKV TCLKWVNLAS SDTVAIILGM NNGEIWLYS VLANEVTYKF TTGNSYEIKD IDLMGNQLWC IDSSDAFYQF DLLQFKLLQH FRINNCVQLN KLTIVPAGDS V AQLLVASH SISLIDIEEK KVVMTFPGHV SPVSTLQVIT NEFFISGAEG DRFLNVYDIH SGMTKCVLVA ESDIKELSHS GQ ADSIAVT TEDGSLEIFV DPLVSSSTKK RGNKSKKSSK KIQIVSKDGR KVPIYNAFIN KDLLNVSWLQ NATMPYFKNL QWR EIPNEY TVEISLNWNN KNKSADRDLH GKDLASATNY VEGNARVTSG DNFKHVDDAI KSWERELTSL EQEQAKPPQA NELL TETFG DKLESSTVAR ISGKKTNLKG SNLKTATTTG TVTVILSQAL QSNDHSLLET VLNNRDERVI RDTIFRLKPA LAVIL LERL AERIARQTHR QGPLNVWVKW CLIIHGGYLV SIPNLMSTLS SLHSTLKRRS DLLPRLLALD ARLDCTINKF KTLNYE AGD IHSSEPVVEE DEDDVEYNEE LDDAGLIEDG EESYGSEEEE EGDSDNEEEQ KHTSSKQDGR LETEQSDGEE EAGYSDV EM E

UniProtKB: U3 small nucleolar RNA-associated protein 5

Macromolecule #21: U3 small nucleolar RNA-associated protein 8

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 8 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 80.269648 KDa

Sequence

String:

MPSLSQPFRL ATLPKIASLS NFSLQADYVQ VADGTFNEST NNITLGISGS SISQYIINPT PKLTFDYPIP STNIITACNA EKGQANIDG NIEASTDDEA NNEKTINTQK KRNVEIWAFG LMVNKGNYTL NVITKALEDT TDTSNDHLSE SDIDNKAYTG S DEFLSQYK IKAKAKVMSI KIDTKNSLVI AILQNGLIEI FDFKLTLLHS FDISYDNLKY AKWFTENGTE YVFVLCPLQD DK VCYKLLE LTDCGSGESS PIKELSSTII EGFSFENSKL CYQFGKLYKL NQGKIYIYSL PHCQLQQVIE FPMVDKLSPG DDL ISFQPV SVNRVLLTVN NVIYLLDLLH CSTLSQRELT HVKTFQLLKS AVINSEKSHN SKTIAIGIST KNGPNPTSSL EIIN IDVGT NTLKDSLGKS FQVGNNDSSV ILKPLFDDKD INDKRVKCND VSGDSSVPVL HCNEVIEKLS ALQDNDITSF DDIFF KELK IKEEHYTEKD RYISDPGFLN KVLDLIFGKF SGNDYPKTLT FLLTHPLFPL SRTRNLLSLL RDQPRLFKQA IVTCPN LPL NELLEELFSI RNRELLLDIS FRILQDFTRD SIKQEMKKLS KLDVQNFIEF ITSGGEDSSP ECFNPSQSTQ LFQLLSL VL DSIGLFSLEG ALLENLTLYI DKQVEIAERN TELWNLIDTK GFQHGFASST FDNGTSQKRA LPTYTMEYLD I

UniProtKB: U3 small nucleolar RNA-associated protein 8

Macromolecule #22: U3 small nucleolar RNA-associated protein 9

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 9 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 65.347254 KDa

Sequence

String:

MGSSLDLVAS FSHDSTRFAF QASVAQKNNV DIYPLNETKD YVVNSSLVSH IDYETNDMKV SDVIFFGWCS DLIDTQSSNI KRKLDEDEG TGESSEQRCE NFFVNGFPDG RIVVYSSNGK DIVNIIKNKK EILGADTDES DIWILDSDKV VKKLQYNNSK P LKTFTLVD GKDDEIVHFQ ILHQNGTLLV CIITKQMVYI VDPSKRRPST KYSFEISDAV ACEFSSDGKY LLIANNEELI AY DLKEDSK LIQSWPVQVK TLKTLDDLIM ALTTDGKINN YKIGEADKVC SIVVNEDLEI IDFTPINSKQ QVLISWLNVN EPN FESISL KEIETQGYIT INKNEKNNAD EADQKKLEEK EEEAQPEVQH EKKETETKIN KKVSKSDQVE IANILSSHLE ANST EILDD LMSGSWTEPE IKKFILTKIN TVDHLSKIFL TISKSITQNP WNEENLLPLW LKWLLTLKSG ELNSIKDKHT KKNCK HLKS ALRSSEEILP VLLGIQGRLE MLRRQAKLRE DLAQLSMQEG EDDEIEVIEH SNVISNPLQD QASPVEKLEP DSIVYA NGE SDEFVDASEY KD

UniProtKB: U3 small nucleolar RNA-associated protein 9

Macromolecule #23: U3 small nucleolar RNA-associated protein 10

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 10 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 200.298984 KDa

Sequence

String:

MSSLSDQLAQ VASNNATVAL DRKRRQKLHS ASLIYNSKTA ATQDYDFIFE NASKALEELS QIEPKFAIFS RTLFSESSIS LDRNVQTKE EIKDLDNAIN AYLLLASSKW YLAPTLHATE WLVRRFQIHV KNTEMLLLST LNYYQTPVFK RILSIIKLPP L FNCLSNFV RSEKPPTALT MIKLFNDMDF LKLYTSYLDQ CIKHNATYTN QLLFTTCCFI NVVAFNSNND EKLNQLVPIL LE ISAKLLA SKSKDCQIAA HTILVVFATA LPLKKTIILA AMETILSNLD AKEAKHSALL TICKLFQTLK GQGNVDQLPS KIF KLFDSK FDTVSILTFL DKEDKPVCDK FITSYTRSIA RYDRSKLNII LSLLKKIRLE RYEVRLIITD LIYLSEILED KSQL VELFE YFISINEDLV LKCLKSLGLT GELFEIRLTT SLFTNADVNT DIVKQLSDPV ETTKKDTASF QTFLDKHSEL INTTN VSML TETGERYKKV LSLFTEAIGK GYKASSFLTS FFTTLESRIT FLLRVTISPA APTALKLISL NNIAKYINSI EKEVNI FTL VPCLICALRD ASIKVRTGVK KILSLIAKRP STKHYFLSDK LYGENVTIPM LNPKDSEAWL SGFLNEYVTE NYDISRI LT PKRNEKVFLM FWANQALLIP SPYAKTVLLD NLNKSPTYAS SYSSLFEEFI SHYLENRSSW EKSCIANKTN FEHFERSL V NLVSPKEKQS FMIDFVLSAL NSDYEQLANI AAERLISIFA SLNNAQKLKI VQNIVDSSSN VESSYDTVGV LQSLPLDSD IFVSILNQNS ISNEMDQTDF SKRRRRRSST SKNAFLKEEV SQLAELHLRK LTIILEALDK VRNVGSEKLL FTLLSLLSDL ETLDQDGGL PVLYAQETLI SCTLNTITYL KEHGCTELTN VRADILVSAI RNSASPQVQN KLLLVIGSLA TLSSEVILHS V MPIFTFMG AHSIRQDDEF TTKVVERTIL TVVPALIKNS KGNEKEEMEF LLLSFTTALQ HVPRHRRVKL FSTLIKTLDP VK ALGSFLF LIAQQYSSAL VNFKIGEARI LIEFIKALLV DLHVNEELSG LNDLLDIIKL LTSSKSSSEK KKSLESRVLF SNG VLNFSE SEFLTFMNNT FEFINKITEE TDQDYYDVRR NLRLKVYSVL LDETSDKKLI RNIREEFGTL LEGVLFFINS VELT FSCIT SQENEEASDS ETSLSDHTTE IKEILFKVLG NVLQILPVDE FVNAVLPLLS TSTNEDIRYH LTLVIGSKFE LEGSE AIPI VNNVMKVLLD RMPLESKSVV ISQVILNTMT ALVSKYGKKL EGSILTQALT LATEKVSSDM TEVKISSLAL ITNCVQ VLG VKSIAFYPKI VPPSIKLFDA SLADSSNPLK EQLQVAILLL FAGLIKRIPS FLMSNILDVL HVIYFSREVD SSIRLSV IS LIIENIDLKE VLKVLFRIWS TEIATSNDTV AVSLFLSTLE STVENIDKKS ATSQSPIFFK LLLSLFEFRS ISSFDNNT I SRIEASVHEI SNSYVLKMND KVFRPLFVIL VRWAFDGEGV TNAGITETER LLAFFKFFNK LQENLRGIIT SYFTYLLEP VDMLLKRFIS KDMENVNLRR LVINSLTSSL KFDRDEYWKS TSRFELISVS LVNQLSNIEN SIGKYLVKAI GALASNNSGV DEHNQILNK LIVEHMKASC SSNEKLWAIR AMKLIYSKIG ESWLVLLPQL VPVIAELLED DDEEIEREVR TGLVKVVENV L GEPFDRYL D

UniProtKB: U3 small nucleolar RNA-associated protein 10

Macromolecule #24: U3 small nucleolar RNA-associated protein 15

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 15 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 57.765289 KDa

Sequence

String:

MSTARPRIIT SKAPLLPQQT TPEQRYWRQY TSAQLVKEHN SVTHISFNPQ HPHDFAVTSS TRVQIFSSRT RQVIKTFSRF KDVVYSASF RSDGKLLCAG DATGLVSVYD SYNPRTILLS INASTHPTHV TKFHTQDNKI LATASDDRVT RLWDISNAYE P QLELTGAT DYVRTLSFIP AAPHLVATGS YDGLIRLYDT RSSGSTPIYS LNHDQPVENV IAVSPTQIVS CGGNNFKVWD LT SNKKLYE RGNFNKAVTC LDYVENFDSP MQSALIASSL DGHVKVFDPL DNFQVKFGWK FSGPVLSCAV SPSTAQGNRH LVA GLSSGL LAIRTKKKEK RSSDKENAPA SFNKNAKSNN FQRMMRGSEY QGDQEHIIHN DKVRSQRRMR AFERNINQFK WSEA LDNAF VPGMAKELTL TVLQELRKRG KVRVALYGRD ESTLEPLLNW CLKGIEDVRS ASIVADWVAV VLELYGNTLE SSPVL QELM IDLKTKVRHE IHKSKEAQRI EGMLQLLTS

UniProtKB: U3 small nucleolar RNA-associated protein 15

Macromolecule #25: NET1-associated nuclear protein 1

• Macromolecule: Name: NET1-associated nuclear protein 1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 101.341734 KDa

Sequence

String:

MTQSLGIEQY KLSVVSGGKP ALNNLSSVTG NKNIARLSQD QRNYIIPFNN QIKVYSVETR QCVKTLKFAN NSLLSGIFLQ EEENNESIV KILLGDITVP QQEDAHLITV FTNNGHVIVL NYKGKLVESP KHFKISLADE KLANVFHSEG NYRILTTFKD P SQKAHNSL QSYRLYALTF DDAKKQFEVA HQAEWHNVIL SNISSNGKLL AHMCKDVSTK DHEHKSISVV SLFDDSVNLS FP LGSILSS QTQSLSYNTR YVSSMAIDNM GQQLAVGFAS GVISIVSLAD LQIRLLKWHI DSVLSLSFSH DGSYLLSGGW EKV MSLWQL ETNSQQFLPR LNGIIIDCQV LGPQGNYYSL ILQMTENNSN SDYQFLLLNA SDLTSKLSIN GPLPVFNSTI KHIQ QPISA MNTKNSNSIT SLNHSKKKQS RKLIKSRRQD FTTNVEINPI NKNLYFPHIS AVQIFDFYKN EQVNYQYLTS GVNNS MGKV RFELNLQDPI ITDLKFTKDG QWMITYEIEY PPNDLLSSKD LTHILKFWTK NDNETNWNLK TKVINPHGIS VPITKI LPS PRSVNNSQGC LTADNNGGLK FWSFDSHESN WCLKKISLPN FNHFSNSVSL AWSQDGSLIF HGFDDKLQIL DFDTFKK FE SLENTKTVSE FTLDSEIQTV KLINDTNLIV ATRTTLNAIN LLRGQVINSF DLYPFVNGVY KNGHMDRLIT CDERTGNI A LVINQQLTDL DGVPTINYKS RIIIFDSDLS TKLGNFTHHE YISWIGWNYD TDFIFLDIES TLGVVGTTVN TQLSDEVNN EGILDGLVSN TITTSASNSD IFAEQLHKLS SRGKKSDTRD KNTNDNDEDE EDIALEFING EKKDKLVNMN SFTSMFDNIQ NVQMDTFFD RVMKVLT

UniProtKB: NET1-associated nuclear protein 1

Macromolecule #26: Periodic tryptophan protein 2

• Macromolecule: Name: Periodic tryptophan protein 2 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 104.097039 KDa

Sequence

String:

MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF EYEHRKNIAA IDLNKQGTLL ISIDEDGRAI LVNFKARNV LHHFNFKEKC SAVKFSPDGR LFALASGRFL QIWKTPDVNK DRQFAPFVRH RVHAGHFQDI TSLTWSQDSR F ILTTSKDL SAKIWSVDSE EKNLAATTFN GHRDYVMGAF FSHDQEKIYT VSKDGAVFVW EFTKRPSDDD DNESEDDDKQ EE VDISKYS WRITKKHFFY ANQAKVKCVT FHPATRLLAV GFTSGEFRLY DLPDFTLIQQ LSMGQNPVNT VSVNQTGEWL AFG SSKLGQ LLVYEWQSES YILKQQGHFD STNSLAYSPD GSRVVTASED GKIKVWDITS GFCLATFEEH TSSVTAVQFA KRGQ VMFSS SLDGTVRAWD LIRYRNFRTF TGTERIQFNC LAVDPSGEVV CAGSLDNFDI HVWSVQTGQL LDALSGHEGP VSCLS FSQE NSVLASASWD KTIRIWSIFG RSQQVEPIEV YSDVLALSMR PDGKEVAVST LKGQISIFNI EDAKQVGNID CRKDII SGR FNQDRFTAKN SERSKFFTTI HYSFDGMAIV AGGNNNSICL YDVPNEVLLK RFIVSRNMAL NGTLEFLNSK KMTEAGS LD LIDDAGENSD LEDRIDNSLP GSQRGGDLST RKMRPEVRVT SVQFSPTANA FAAASTEGLL IYSTNDTILF DPFDLDVD V TPHSTVEALR EKQFLNALVM AFRLNEEYLI NKVYEAIPIK EIPLVASNIP AIYLPRILKF IGDFAIESQH IEFNLIWIK ALLSASGGYI NEHKYLFSTA MRSIQRFIVR VAKEVVNTTT DNKYTYRFLV STDGSMEDGA ADDDEVLLKD DADEDNEENE ENDVVMESD DEEGWIGFNG KDNKLPLSNE NDSSDEEENE KELP

UniProtKB: Periodic tryptophan protein 2

Macromolecule #27: U3 small nucleolar RNA-associated protein 12

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 12 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 106.481133 KDa

Sequence

String:

MVKSYQRFEQ AAAFGVIASN ANCVWIPASS GNSNGSGPGQ LITSALEDVN IWDIKTGDLV SKLSDGLPPG ASDARGAKPA ECTYLEAHK DTDLLAVGYA DGVIKVWDLM SKTVLLNFNG HKAAITLLQF DGTGTRLISG SKDSNIIVWD LVGEVGLYKL R SHKDSITG FWCQGEDWLI STSKDGMIKL WDLKTHQCIE THIAHTGECW GLAVKDDLLI TTGTDSQVKI WKLDIENDKM GG KLTEMGI FEKQSKQRGL KIEFITNSSD KTSFFYIQNA DKTIETFRIR KEEEIARGLK KREKRLKEKG LTEEEIAKSI KES YSSFIL HPFQTIRSLY KIKSASWTTV SSSKLELVLT TSSNTIEYYS IPYEKRDPTS PAPLKTHTIE LQGQRTDVRS IDIS DDNKL LATASNGSLK IWNIKTHKCI RTFECGYALT CKFLPGGLLV ILGTRNGELQ LFDLASSSLL DTIEDAHDAA IWSLD LTSD GKRLVTGSAD KTVKFWDFKV ENSLVPGTKN KFLPVLKLHH DTTLELTDDI LCVRVSPDDR YLAISLLDNT VKVFFL DSM KFYLSLYGHK LPVLSIDISF DSKMIITSSA DKNIKIWGLD FGDCHKSLFA HQDSIMNVKF LPQSHNFFSC SKDAVVK YW DGEKFECIQK LYAHQSEVWA LAVATDGGFV VSSSHDHSIR IWEETEDQVF LEEEKEKELE EQYEDTLLTS LEEGNGDD A FKADASGEGV EDEASGVHKQ TLESLKAGER LMEALDLGIA EIEGLEAYNR DMKLWQRKKL GEAPIKPQGN AVLIAVNKT PEQYIMDTLL RIRMSQLEDA LMVMPFSYVL KFLKFIDTVM QNKTLLHSHL PLICKNLFFI IKFNHKELVS QKNEELKLQI NRVKTELRS ALKSTEDDLG FNVQGLKFVK QQWNLRHNYE FVDEYDQQEK ESNSARKRVF GTVI

UniProtKB: U3 small nucleolar RNA-associated protein 12

Macromolecule #28: U3 small nucleolar RNA-associated protein 13

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 13 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 91.132562 KDa

Sequence

String:

MDLKTSYKGI SLNPIYAGSS AVATVSENGK ILATPVLDEI NIIDLTPGSR KILHKISNED EQEITALKLT PDGQYLTYVS QAQLLKIFH LKTGKVVRSM KISSPSYILD ADSTSTLLAV GGTDGSIIVV DIENGYITHS FKGHGGTISS LKFYGQLNSK I WLLASGDT NGMVKVWDLV KRKCLHTLQE HTSAVRGLDI IEVPDNDEPS LNLLSGGRDD IINLWDFNMK KKCKLLKTLP VN QQVESCG FLKDGDGKRI IYTAGGDAIF QLIDSESGSV LKRTNKPIEE LFIIGVLPIL SNSQMFLVLS DQTLQLINVE EDL KNDEDT IQVTSSIAGN HGIIADMRYV GPELNKLALA TNSPSLRIIP VPDLSGPEAS LPLDVEIYEG HEDLLNSLDA TEDG LWIAT ASKDNTAIVW RYNENSCKFD IYAKYIGHSA AVTAVGLPNI VSKGYPEFLL TASNDLTIKK WIIPKPTASM DVQII KVSE YTRHAHEKDI NALSVSPNDS IFATASYDKT CKIWNLENGE LEATLANHKR GLWDVSFCQY DKLLATSSGD KTVKIW SLD TFSVMKTLEG HTNAVQRCSF INKQKQLISC GADGLIKIWD CSSGECLKTL DGHNNRLWAL STMNDGDMIV SADADGV FQ FWKDCTEQEI EEEQEKAKLQ VEQEQSLQNY MSKGDWTNAF LLAMTLDHPM RLFNVLKRAL GESRSRQDTE EGKIEVIF N EELDQAISIL NDEQLILLMK RCRDWNTNAK THTIAQRTIR CILMHHNIAK LSEIPGMVKI VDAIIPYTQR HFTRVDNLV EQSYILDYAL VEMDKLF

UniProtKB: U3 small nucleolar RNA-associated protein 13

Macromolecule #29: U3 small nucleolar RNA-associated protein 18

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 18 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 66.49425 KDa

Sequence

String:

MTMATTAMNV SVPPPDEEEQ LLAKFVFGDT TDLQENLAKF NADFIFNEQE MDVEDQEDEG SESDNSEEDE AQNGELDHVN NDQLFFVDD GGNEDSQDKN EDTMDVDDED DSSSDDYSED SEEAAWIDSD DEKIKVPILV TNKTKKLRTS YNESKINGVH Y INRLRSQF EKIYPRPKWV DDESDSELDD EEDDEEEGSN NVINGDINAL TKILSTTYNY KDTLSNSKLL PPKKLDIVRL KD ANASHPS HSAIQSLSFH PSKPLLLTGG YDKTLRIYHI DGKTNHLVTS LHLVGSPIQT CTFYTSLSNQ NQQNIFTAGR RRY MHSWDL SLENLTHSQT AKIEKFSRLY GHESTQRSFE NFKVAHLQNS QTNSVHGIVL LQGNNGWINI LHSTSGLWLM GCKI EGVIT DFCIDYQPIS RGKFRTILIA VNAYGEVWEF DLNKNGHVIR RWKDQGGVGI TKIQVGGGTT TTCPALQISK IKQNR WLAV GSESGFVNLY DRNNAMTSST PTPVAALDQL TTTISNLQFS PDGQILCMAS RAVKDALRLV HLPSCSVFSN WPTSGT PLG KVTSVAFSPS GGLLAVGNEQ GKVRLWKLNH Y

UniProtKB: U3 small nucleolar RNA-associated protein 18

Macromolecule #30: U3 small nucleolar RNA-associated protein 21

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 21 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 104.927844 KDa

Sequence

String:

MSIDLKKRKV EEDVRSRGKN SKIFSPFRII GNVSNGVPFA TGTLGSTFYI VTCVGKTFQI YDANTLHLLF VSEKETPSSI VALSAHFHY VYAAYENKVG IYKRGIEEHL LELETDANVE HLCIFGDYLC ASTDDNSIFI YKKSDPQDKY PSEFYTKLTV T EIQGGEIV SLQHLATYLN KLTVVTKSNV LLFNVRTGKL VFTSNEFPDQ ITTAEPAPVL DIIALGTVTG EVIMFNMRKG KR IRTIKIP QSRISSLSFR TDGSSHLSVG TSSGDLIFYD LDRRSRIHVL KNIHRESYGG VTQATFLNGQ PIIVTSGGDN SLK EYVFDP SLSQGSGDVV VQPPRYLRSR GGHSQPPSYI AFADSQSHFM LSASKDRSLW SFSLRKDAQS QEMSQRLHKK QDGG RVGGS TIKSKFPEIV ALAIENARIG EWENIITAHK DEKFARTWDM RNKRVGRWTF DTTDDGFVKS VAMSQCGNFG FIGSS NGSI TIYNMQSGIL RKKYKLHKRA VTGISLDGMN RKMVSCGLDG IVGFYDFNKS TLLGKLKLDA PITAMVYHRS SDLFAL ALD DLSIVVIDAV TQRVVRQLWG HSNRITAFDF SPEGRWIVSA SLDSTIRTWD LPTGGCIDGI IVDNVATNVK FSPNGDL LA TTHVTGNGIC IWTNRAQFKT VSTRTIDESE FARMALPSTS VRGNDSMLSG ALESNGGEDL NDIDFNTYTS LEQIDKEL L TLSIGPRSKM NTLLHLDVIR KRSKPKEAPK KSEKLPFFLQ LSGEKVGDEA SVREGIAHET PEEIHRRDQE AQKKLDAEE QMNKFKVTGR LGFESHFTKQ LREGSQSKDY SSLLATLINF SPAAVDLEIR SLNSFEPFDE IVWFIDALTQ GLKSNKNFEL YETFMSLLF KAHGDVIHAN NKNQDIASAL QNWEDVHKKE DRLDDLVKFC MGVAAFVTTA

UniProtKB: U3 small nucleolar RNA-associated protein 21

Macromolecule #31: U3 small nucleolar RNA-associated protein 6

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 6 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 52.495277 KDa

Sequence

String:

MSKTRYYLEQ CIPEMDDLVE KGLFTKNEVS LIMKKRTDFE HRLNSRGSSI NDYIKYINYE SNVNKLRAKR CKRILQVKKT NSLSDWSIQ QRIGFIYQRG TNKFPQDLKF WAMYLNYMKA RGNQTSYKKI HNIYNQLLKL HPTNVDIWIS CAKYEYEVHA N FKSCRNIF QNGLRFNPDV PKLWYEYVKF ELNFITKLIN RRKVMGLINE REQELDMQNE QKNNQAPDEE KSHLQVPSTG DS MKDKLNE LPEADISVLG NAETNPALRG DIALTIFDVC MKTLGKHYIN KHKGYYAISD SKMNIELNKE TLNYLFSESL RYI KLFDEF LDLERDYLIN HVLQFWKNDM YDLSLRKDLP ELYLKTVMID ITLNIRYMPV EKLDIDQLQL SVKKYFAYIS KLDS ASVKS LKNEYRSYLQ DNYLKKMNAE DDPRYKILDL IISKL

UniProtKB: U3 small nucleolar RNA-associated protein 6

Macromolecule #32: Bud site selection protein 21

• Macromolecule: Name: Bud site selection protein 21 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 24.431016 KDa

Sequence

String:

MSNGHVKFDA DESQASASAV TDRQDDVLVI SKKDKEVHSS SDEESDDDDA PQEEGLHSGK SEVESQITQR EEAIRLEQSQ LRSKRRKQN ELYAKQKKSV NETEVTDEVI AELPEELLKN IDQKDEGSTQ YSSSRHVTFD KLDESDENEE ALAKAIKTKK R KTLKNLRK DSVKRGKFRV QLLSTTQDSK TLPPKKESSI IRSKDRWLNR KALNKG

UniProtKB: U3 small nucleolar RNA-associated protein 16

Macromolecule #33: U3 small nucleolar RNA-associated protein 7

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 7 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 62.41857 KDa

Sequence

String:

MGHKKNGHRR QIKERENQNK FERSTYTNNA KNNHTQTKDK KLRAGLKKID EQYKKAVSSA AATDYLLPES NGYLEPENEL EKTFKVQQS EIKSSVDVST ANKALDLSLK EFGPYHIKYA KNGTHLLITG RKGHVASMDW RKGQLRAELF LNETCHSATY L QNEQYFAV AQKKYTFIYD HEGTELHRLK QHIEARHLDF LPYHYLLVTA GETGWLKYHD VSTGQLVSEL RTKAGPTMAM AQ NPWNAVM HLGHSNGTVS LWSPSMPEPL VKLLSARGPV NSIAIDRSGY YMATTGADRS MKIWDIRNFK QLHSVESLPT PGT NVSISD TGLLALSRGP HVTLWKDALK LSGDSKPCFG SMGGNPHRNT PYMSHLFAGN KVENLGFVPF EDLLGVGHQT GITN LIVPG AGEANYDALE LNPFETKKQR QEQEVRTLLN KLPADTITLD PNSIGSVDKR SSTIRLNAKD LAQTTMDANN KAKTN SDIP DVKPDVKGKN SGLRSFLRKK TQNVIDERKL RVQKQLDKEK NIRKRNHQIK QGLISEDHKD VIEEALSRFG

UniProtKB: U3 small nucleolar RNA-associated protein 7

Macromolecule #34: U3 small nucleolar RNA-associated protein 11

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 11 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 29.806348 KDa

Sequence

String:

MAKLVHDVQK KQHRERSQLT SRSRYGFLEK HKDYVKRAQD FHRKQSTLKV LREKAKERNP DEYYHAMHSR KTDAKGLLIS SRHGDEEDE SLSMDQVKLL KTQDSNYVRT LRQIELKKLE KGAKQLMFKS SGNHTIFVDS REKMNEFTPE KFFNTTSEMV N RSENRLTK DQLAQDISNN RNASSIMPKE SLDKKKLKKF KQVKQHLQRE TQLKQVQQRM DAQRELLKKG SKKKIVDSSG KI SFKWKKQ RKR

UniProtKB: U3 small nucleolar RNA-associated protein 11

Macromolecule #35: U3 small nucleolar RNA-associated protein MPP10

• Macromolecule: Name: U3 small nucleolar RNA-associated protein MPP10 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 67.042492 KDa

Sequence

String:

MSELFGVLKS NAGRIILKDP SATSKDVKAY IDSVINTCKN GSITKKAELD EITVDGLDAN QVWWQVKLVL DSIDGDLIQG IQELKDVVT PSHNLSDGST LNSSSGEESE LEEAESVFKE KQMLSADVSE IEEQSNDSLS ENDEEPSMDD EKTSAEAARE E FAEEKRIS SGQDERHSSP DPYGINDKFF DLEKFNRDTL AAEDSNEASE GSEDEDIDYF QDMPSDDEEE EAIYYEDFFD KP TKEPVKK HSDVKDPKED EELDEEEHDS AMDKVKLDLF ADEEDEPNAE GVGEASDKNL SSFEKQQIEI RKQIEQLENE AVA EKKWSL KGEVKAKDRP EDALLTEELE FDRTAKPVPV ITSEVTESLE DMIRRRIQDS NFDDLQRRTL LDITRKSQRP QFEL SDVKS SKSLAEIYED DYTRAEDESA LSEELQKAHS EISELYANLV YKLDVLSSVH FVPKPASTSL EIRVETPTIS MEDAQ PLYM SNASSLAPQE IYNVGKAEKD GEIRLKNGVA MSKEELTRED KNRLRRALKR KRSKANLPNV NKRSKRNDVV DTLSKA KNI TVINQKGEKK DVSGKTKKSR SGPDSTNIKL

UniProtKB: U3 small nucleolar RNA-associated protein MPP10

Macromolecule #36: U3 small nucleolar ribonucleoprotein protein IMP3

• Macromolecule: Name: U3 small nucleolar ribonucleoprotein protein IMP3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.928529 KDa

Sequence

String:

MVRKLKHHEQ KLLKKVDFLE WKQDQGHRDT QVMRTYHIQN REDYHKYNRI CGDIRRLANK LSLLPPTDPF RRKHEQLLLD KLYAMGVLT TKSKISDLEN KVTVSAICRR RLPVIMHRLK MAETIQDAVK FIEQGHVRVG PNLINDPAYL VTRNMEDYVT W VDNSKIKK TLLRYRNQID DFDFS

UniProtKB: U3 small nucleolar ribonucleoprotein protein IMP3

Macromolecule #37: U3 small nucleolar ribonucleoprotein protein IMP4

• Macromolecule: Name: U3 small nucleolar ribonucleoprotein protein IMP4 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 33.536168 KDa

Sequence

String:

MLRRQARERR EYLYRKAQEL QDSQLQQKRQ IIKQALAQGK PLPKELAEDE SLQKDFRYDQ SLKESEEADD LQVDDEYAAT SGIMDPRII VTTSRDPSTR LSQFAKEIKL LFPNAVRLNR GNYVMPNLVD ACKKSGTTDL VVLHEHRGVP TSLTISHFPH G PTAQFSLH NVVMRHDIIN AGNQSEVNPH LIFDNFTTAL GKRVVCILKH LFNAGPKKDS ERVITFANRG DFISVRQHVY VR TREGVEI AEVGPRFEMR LFELRLGTLE NKDADVEWQL RRFIRTANKK DYL

UniProtKB: U3 small nucleolar ribonucleoprotein protein IMP4

Macromolecule #38: Something about silencing protein 10

• Macromolecule: Name: Something about silencing protein 10 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 70.364398 KDa

Sequence

String:

MVRKGSNRTK TSEVGDEINP YGLNEVDDFA SKREKVLLGQ STFGDSNKDD DHSLLEDEDE EEVLAMDEDD ESIDEREDEE EEEEEELDG AAAYKKIFGR NLETDQLPEE DEENGMLDNE NAWGSTKGEY YGADDLDDDE AAKEIEKEAL RQQKKHLEEL N MNDYLDEE EEEEWVKSAK EFDMGEFKNS TKQADTKTSI TDILNMDDEA RDNYLRTMFP EFAPLSKEFT ELAPKFDELK KS EENEFNK LKLIALGSYL GTISCYYSIL LHELHNNEDF TSMKGHPVME KILTTKEIWR QASELPSSFD VNEGDGSESE ETA NIEAFN EKKLNELQNS EDSDAEDGGK QKQEIDEEER ESDEEEEEED VDIDDFEEYV AQSRLHSKPK TSSMPEADDF IESE IADVD AQDKKARRRT LRFYTSKIDQ QENKKTDRFK GDDDIPYKER LFERQQRLLD EARKRGMHDN NGADLDDKDY GSEDE AVSR SINTQGENDY YQQVQRGKQD KKISRKEAHK NAVIAAREGK LAELAENVSG DGKRAINYQI LKNKGLTPKR NKDNRN SRV KKRKKYQKAQ KKLKSVRAVY SGGQSGVYEG EKTGIKKGLT RSVKFKN

UniProtKB: Something about silencing protein 10

Macromolecule #39: Protein SOF1

• Macromolecule: Name: Protein SOF1 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 56.888918 KDa

Sequence

String:

MKIKTIKRSA DDYVPVKSTQ ESQMPRNLNP ELHPFERARE YTKALNATKL ERMFAKPFVG QLGYGHRDGV YAIAKNYGSL NKLATGSAD GVIKYWNMST REEFVSFKAH YGLVTGLCVT QPRFHDKKPD LKSQNFMLSC SDDKTVKLWS INVDDYSNKN S SDNDSVTN EEGLIRTFDG ESAFQGIDSH RENSTFATGG AKIHLWDVNR LKPVSDLSWG ADNITSLKFN QNETDILAST GS DNSIVLY DLRTNSPTQK IVQTMRTNAI CWNPMEAFNF VTANEDHNAY YYDMRNLSRS LNVFKDHVSA VMDVDFSPTG DEI VTGSYD KSIRIYKTNH GHSREIYHTK RMQHVFQVKY SMDSKYIISG SDDGNVRLWR SKAWERSNVK TTREKNKLEY DEKL KERFR HMPEIKRISR HRHVPQVIKK AQEIKNIELS SIKRREANER RTRKDMPYIS ERKKQIVGTV HKYEDSGRDR KRRKE DDKR DTQEK

UniProtKB: Protein SOF1

Macromolecule #40: rRNA-processing protein FCF2

• Macromolecule: Name: rRNA-processing protein FCF2 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 25.68924 KDa

Sequence

String:

MDQSVEDLFG ALRDASASLE VKNSAKEQVS LQQEDVLQIG NNDDEVEIES KFQEIETNLK KLPKLETGFD ALANKKKKKN VLPSVETED KRKPNKSDKN DNDWFTLPKP DDNMRREVQR DLLLIKHRAA LDPKRHYKKQ RWEVPERFAI GTIIEDKSEF Y SSRMNRKE RKSTILETLM GDEASNKYFK RKYNEIQEKS TSGRKAHYKK MKEMRKKRR

UniProtKB: rRNA-processing protein FCF2

Macromolecule #41: rRNA-processing protein FCF1

• Macromolecule: Name: rRNA-processing protein FCF1 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 21.650729 KDa

Sequence

String:

MGKAKKTRKF GLVKRTLNTK KDQRLKKNQE NIKTKEDPEL TRNIPQVSSA LFFQYNQAIK PPYQVLIDTN FINFSIQKKV DIVRGMMDC LLAKCNPLIT DCVMAELEKL GPKYRIALKL ARDPRIKRLS CSHKGTYADD CLVHRVLQHK CYIVATNDAG L KQRIRKIP GIPLMSVGGH AYVIEKLPDV F

UniProtKB: rRNA-processing protein FCF1

Macromolecule #42: Ribosome biogenesis protein ENP2

• Macromolecule: Name: Ribosome biogenesis protein ENP2 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 81.864172 KDa

Sequence

String:

MVLKSTSAND VSVYQVSGTN VSRSLPDWIA KKRKRQLKND LEYQNRVELI QDFEFSEASN KIKVSRDGQY CMATGTYKPQ IHVYDFANL SLKFDRHTDA ENVDFTILSD DWTKSVHLQN DRSIQFQNKG GLHYTTRIPK FGRSLVYNKV NCDLYVGASG N ELYRLNLE KGRFLNPFKL DTEGVNHVSI NEVNGLLAAG TETNVVEFWD PRSRSRVSKL YLENNIDNRP FQVTTTSFRN DG LTFACGT SNGYSYIYDL RTSEPSIIKD QGYGFDIKKI IWLDNVGTEN KIVTCDKRIA KIWDRLDGKA YASMEPSVDI NDI EHVPGT GMFFTANESI PMHTYYIPSL GPSPRWCSFL DSITEELEEK PSDTVYSNYR FITRDDVKKL NLTHLVGSRV LRAY MHGFF INTELYDKVS LIANPDAYKD EREREIRRRI EKERESRIRS SGAVQKPKIK VNKTLVDKLS QKRGDKVAGK VLTDD RFKE MFEDEEFQVD EDDYDFKQLN PVKSIKETEE GAAKRIRALT AAEESDEERI AMKDGRGHYD YEDEESDEEE SDDETN QKS NKEELSEKDL RKMEKQKALI ERRKKEKEQS ERFMNEMKAG TSTSTQRDES AHVTFGEQVG ELLEVENGKK SNESILR RN QRGEAELTFI PQRKSKKDGN YKSRRHDNSS DEEGIDENGN KKDNGRSKPR FENRRRASKN AFRGM

UniProtKB: Ribosome biogenesis protein ENP2

Macromolecule #43: U3 small nucleolar ribonucleoprotein protein LCP5

• Macromolecule: Name: U3 small nucleolar ribonucleoprotein protein LCP5 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 40.864391 KDa

Sequence

String:

MSELNALLKD INGSLTATSE SLERLSGIYS NSATDEIPES NQLHEHLFYD AKKPAEKVSL LSLKNGSMLG YINSLLMLIG NRLDDECKD PSAMDARERS IQHRVVLERG VKPLEKKLAY QLDKLTRAYV KMEKEYKDAE KRALEKSTLV NHSGNDDSED D ESSEDEIA YRPNTSGIIN TNKKSSAYRV EETAKQENGE ENDDNETGVY KPPKITAVLP PQQTHFEDRF DAREHKDRSN KS RMQAMEE YIRESSDQPD WSASIGADIV NHGRGGIKSL RDTEKERRVT SFEEDNFTRL NITNKAEKRK QKQRERNARM NVI GGEDFG IFSSKRKLED STSRRGAKKT RSAWDRAQRR L

UniProtKB: U3 small nucleolar ribonucleoprotein protein LCP5

Macromolecule #44: Ribosomal RNA small subunit methyltransferase NEP1

• Macromolecule: Name: Ribosomal RNA small subunit methyltransferase NEP1 / type: protein_or_peptide / ID: 44 / Number of copies: 2 / Enantiomer: LEVO; EC number: rRNA small subunit pseudouridine methyltransferase Nep1
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 27.936461 KDa

Sequence

String:

MVEDSRVRDA LKGGDQKALP ASLVPQAPPV LTSKDKITKR MIVVLAMASL ETHKISSNGP GGDKYVLLNC DDHQGLLKKM GRDISEARP DITHQCLLTL LDSPINKAGK LQVYIQTSRG ILIEVNPTVR IPRTFKRFSG LMVQLLHKLS IRSVNSEEKL L KVIKNPIT DHLPTKCRKV TLSFDAPVIR VQDYIEKLDD DESICVFVGA MARGKDNFAD EYVDEKVGLS NYPLSASVAC SK FCHGAED AWNIL

UniProtKB: Ribosomal RNA small subunit methyltransferase NEP1

Macromolecule #45: Ribosome biogenesis protein BMS1

• Macromolecule: Name: Ribosome biogenesis protein BMS1 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 135.792281 KDa

Sequence

String:

MEQSNKQHRK AKEKNTAKKK LHTQGHNAKA FAVAAPGKMA RTMQRSSDVN ERKLHVPMVD RTPEDDPPPF IVAVVGPPGT GKTTLIRSL VRRMTKSTLN DIQGPITVVS GKHRRLTFLE CPADDLNAMI DIAKIADLVL LLIDGNFGFE METMEFLNIA Q HHGMPRVL GVATHLDLFK SQSTLRASKK RLKHRFWTEV YQGAKLFYLS GVINGRYPDR EILNLSRFIS VMKFRPLKWR NE HPYMLAD RFTDLTHPEL IETQGLQIDR KVAIYGYLHG TPLPSAPGTR VHIAGVGDFS VAQIEKLPDP CPTPFYQQKL DDF EREKMK EEAKANGEIT TASTTRRRKR LDDKDKLIYA PMSDVGGVLM DKDAVYIDIG KKNEEPSFVP GQERGEGEKL MTGL QSVEQ SIAEKFDGVG LQLFSNGTEL HEVADHEGMD VESGEESIED DEGKSKGRTS LRKPRIYGKP VQEEDADIDN LPSDE EPYT NDDDVQDSEP RMVEIDFNNT GEQGAEKLAL ETDSEFEESE DEFSWERTAA NKLKKTESKK RTWNIGKLIY MDNISP EEC IRRWRGEDDD SKDESDIEED VDDDFFRKKD GTVTKEGNKD HAVDLEKFVP YFDTFEKLAK KWKSVDAIKE RFLGAGI LG NDNKTKSDSN EGGEELYGDF EDLEDGNPSE QAEDNSDKES EDEDENEDTN GDDDNSFTNF DAEEKKDLTM EQEREMNA A KKEKLRAQFE IEEGENFKED DENNEYDTWY ELQKAKISKQ LEINNIEYQE MTPEQRQRIE GFKAGSYVRI VFEKVPMEF VKNFNPKFPI VMGGLLPTEI KFGIVKARLR RHRWHKKILK TNDPLVLSLG WRRFQTLPIY TTTDSRTRTR MLKYTPEHTY CNAAFYGPL CSPNTPFCGV QIVANSDTGN GFRIAATGIV EEIDVNIEIV KKLKLVGFPY KIFKNTAFIK DMFSSAMEVA R FEGAQIKT VSGIRGEIKR ALSKPEGHYR AAFEDKILMS DIVILRSWYP VRVKKFYNPV TSLLLKEKTE WKGLRLTGQI RA AMNLETP SNPDSAYHKI ERVERHFNGL KVPKAVQKEL PFKSQIHQMK PQKKKTYMAK RAVVLGGDEK KARSFIQKVL TIS KAKDSK RKEQKASQRK ERLKKLAKME EEKSQRDKEK KKEYFAQNGK RTTMGGDDES RPRKMRR

UniProtKB: Ribosome biogenesis protein BMS1

Macromolecule #46: RNA 3'-terminal phosphate cyclase-like protein

• Macromolecule: Name: RNA 3'-terminal phosphate cyclase-like protein / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 40.220559 KDa

Sequence

String:

MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHI CPSSKPVGYF VEPMLYLAPF SKKKFSILFK GITASHNDAG IEAIKWGLMP VMEKFGVREC ALHTLKRGSP P LGGGEVHL VVDSLIAQPI TMHEIDRPII SSITGVAYST RVSPSLVNRM IDGAKKVLKN LQCEVNITAD VWRGENSGKS PG WGITLVA QSKQKGWSYF AEDIGDAGSI PEELGEKVAC QLLEEISKSA AVGRNQLPLA IVYMVIGKED IGRLRINKEQ IDE RFIILL RDIKKIFNTE VFLKPVDEAD NEDMIATIKG IGFTNTSKKI A

UniProtKB: RNA 3'-terminal phosphate cyclase-like protein

Macromolecule #47: RNA cytidine acetyltransferase

• Macromolecule: Name: RNA cytidine acetyltransferase / type: protein_or_peptide / ID: 47 / Number of copies: 2 / Enantiomer: LEVO; EC number: Transferases; Acyltransferases; Transferring groups other than aminoacyl groups
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 119.509445 KDa

Sequence

String:

MAKKAIDSRI PSLIRNGVQT KQRSIFVIVG DRARNQLPNL HYLMMSADLK MNKSVLWAYK KKLLGFTSHR KKRENKIKKE IKRGTREVN EMDPFESFIS NQNIRYVYYK ESEKILGNTY GMCILQDFEA LTPNLLARTI ETVEGGGIVV ILLKSMSSLK Q LYTMTMDV HARYRTEAHG DVVARFNERF ILSLGSNPNC LVVDDELNVL PLSGAKNVKP LPPKEDDELP PKQLELQELK ES LEDVQPA GSLVSLSKTV NQAHAILSFI DAISEKTLNF TVALTAGRGR GKSAALGISI AAAVSHGYSN IFVTSPSPEN LKT LFEFIF KGFDALGYQE HIDYDIIQST NPDFNKAIVR VDIKRDHRQT IQYIVPQDHQ VLGQAELVVI DEAAAIPLPI VKNL LGPYL VFMASTINGY EGTGRSLSLK LIQQLRNQNN TSGRESTQTA VVSRDNKEKD SHLHSQSRQL REISLDEPIR YAPGD PIEK WLNKLLCLDV TLIKNPRFAT RGTPHPSQCN LFVVNRDTLF SYHPVSENFL EKMMALYVSS HYKNSPNDLQ LMSDAP AHK LFVLLPPIDP KDGGRIPDPL CVIQIALEGE ISKESVRNSL SRGQRAGGDL IPWLISQQFQ DEEFASLSGA RIVRIAT NP EYASMGYGSR AIELLRDYFE GKFTDMSEDV RPKDYSIKRV SDKELAKTNL LKDDVKLRDA KTLPPLLLKL SEQPPHYL H YLGVSYGLTQ SLHKFWKNNS FVPVYLRQTA NDLTGEHTCV MLNVLEGRES NWLVEFAKDF RKRFLSLLSY DFHKFTAVQ ALSVIESSKK AQDLSDDEKH DNKELTRTHL DDIFSPFDLK RLDSYSNNLL DYHVIGDMIP MLALLYFGDK MGDSVKLSSV QSAILLAIG LQRKNIDTIA KELNLPSNQT IAMFAKIMRK MSQYFRQLLS QSIEETLPNI KDDAIAEMDG EEIKNYNAAE A LDQMEEDL EEAGSEAVQA MREKQKELIN SLNLDKYAIN DNSEEWAESQ KSLEIAAKAK GVVSLKTGKK RTTEKAEDIY RQ EMKAMKK PRKSKKAAN

UniProtKB: RNA cytidine acetyltransferase

Macromolecule #48: Nucleolar complex protein 14

• Macromolecule: Name: Nucleolar complex protein 14 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 94.463195 KDa

Sequence

String:

MAGSQLKNLK AALKARGLTG QTNVKSKNKK NSKRQAKEYD REEKKKAIAE IREEFNPFEI KAARNKRRDG LPSKTADRIA VGKPGISKQ IGEEQRKRAF EARKMMKNKR GGVIDKRFGE RDKLLTEEEK MLERFTRERQ SQSKRNANLF NLEDDEDDGD M FGDGLTHL GQSLSLEDEL ANDEEDFLAS KRFNEDDAEL QQPQRKKTKA EVMKEVIAKS KFYKQERQKA QGIMEDQIDN LD DNFEDVM SELMMTQPKK NPMEPKTDLD KEYDIKVKEL QLDKRAAPSD RTKTEEEKNA EAEEKKRELE QQRLDRMNGM IEL EEGEER GVEDLDDGFW ENEEDYEDDN DGIADSDDDI KFEDQGRDEG FSQILKKKNI SISCPRTHDA LLDQVKKLDL DDHP KIVKN IIKAYQPKLA EGNKEKLGKF TAVLLRHIIF LSNQNYLKNV QSFKRTQNAL ISILKSLSEK YNRELSEECR DYINE MQAR YKKNHFDALS NGDLVFFSII GILFSTSDQY HLVITPALIL MSQFLEQIKF NSLKRIAFGA VLVRIVSQYQ RISKRY IPE VVYFFQKILL TFIVEKENQE KPLDFENIRL DSYELGLPLD VDFTKKRSTI IPLHTLSTMD TEAHPVDQCV SVLLNVM ES LDATISTVWK SLPAFNEIIL PIQQLLSAYT SKYSDFEKPR NILNKVEKLT KFTEHIPLAL QNHKPVSIPT HAPKYEEN F NPDKKSYDPD RTRSEINKMK AQLKKERKFT MKEIRKDAKF EARQRIEEKN KESSDYHAKM AHIVNTINTE EGAEKNKYE RERKLRGGKK

UniProtKB: Nucleolar complex protein 14

Macromolecule #49: Nucleolar complex protein 4

• Macromolecule: Name: Nucleolar complex protein 4 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 63.707844 KDa

Sequence

String:

MVLLISEIKD IAKRLTAAGD RKQYNSIIKL INELVIPENV TQLEEDETEK NLRFLVMSLF QIFRKLFSRG DLTLPSSKKS TLEKEQFVN WCRKVYEAFK TKLLAIISDI PFETSLGLDS LDVYLQLAEL ESTHFASEKG APFFPNKTFR KLIIALWSSN M GEIEDVKS SGASENLIIV EFTEKYYTKF ADIQYYFQSE FNQLLEDPAY QDLLLKNVGK WLALVNHDKH CSSVDADLEI FV PNPPQAI ENESKFKSNF EKNWLSLLNG QLSLQQYKSI LLILHKRIIP HFHTPTKLMD FLTDSYNLQS SNKNAGVVPI LAL NGLFEL MKRFNLEYPN FYMKLYQIIN PDLMHVKYRA RFFRLMDVFL SSTHLSAHLV ASFIKKLARL TLESPPSAIV TVIP FIYNL IRKHPNCMIM LHNPAFISNP FQTPDQVANL KTLKENYVDP FDVHESDPEL THALDSSLWE LASLMEHYHP NVATL AKIF AQPFKKLSYN MEDFLDWNYD SLLNAESSRK LKTLPTLEFE AFTNVFDNED GDSEASSQGN VYLPGVAW

UniProtKB: Nucleolar complex protein 4

Macromolecule #50: U3 small nucleolar RNA-associated protein 20

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 20 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 287.9155 KDa

Sequence

String:

MAKQRQTTKS SKRYRYSSFK ARIDDLKIEP ARNLEKRVHD YVESSHFLAS FDQWKEINLS AKFTEFAAEI EHDVQTLPQI LYHDKKIFN SLVSFINFHD EFSLQPLLDL LAQFCHDLGP DFLKFYEEAI KTLINLLDAA IEFESSNVFE WGFNCLAYIF K YLSKFLVK KLVLTCDLLI PLLSHSKEYL SRFSAEALSF LVRKCPVSNL REFVRSVFEK LEGDDEQTNL YEGLLILFTE SM TSTQETL HSKAKAIMSV LLHEALTKSS PERSVSLLSD IWMNISKYAS IESLLPVYEV MYQDFNDSLD ATNIDRILKV LTT IVFSES GRKIPDWNKI TILIERIMSQ SENCASLSQD KVAFLFALFI RNSDVKTLTL FHQKLFNYAL TNISDCFLEF FQFA LRLSY ERVFSFNGLK FLQLFLKKNW QSQGKKIALF FLEVDDKPEL QKVREVNFPE EFILSIRDFF VTAEINDSND LFEIY WRAI IFKYSKLQNT EIIIPLLERI FSTFASPDNF TKDMVGTLLK IYRKEDDASG NNLLKTILDN YENYKESLNF LRGWNK LVS NLHPSESLKG LMSHYPSLLL SLTDNFMLPD GKIRYETLEL MKTLMILQGM QVPDLLSSCM VIEEIPLTLQ NARDLTI RI KNVGAEFGKT KTDKLVSSFF LKYLFGLLTV RFSPVWTGVF DTLPNVYTKD EALVWKLVLS FIKLPDENQN LDYYQPLL E DGANKVLWDS SVVRLRDTID TFSHIWSKYS TQNTSIISTT IERRGNTTYP ILIRNQALKV MLSIPQVAEN HFVDIAPFV YNDFKTYKDE EDMENERVIT GSWTEVDRNV FLKTLSKFKN IKNVYSATEL HDHLMVLLGS RNTDVQKLAL DALLAYKNPT LNKYRDNLK NLLDDTLFKD EITTFLTENG SQSIKAEDEK VVMPYVLRIF FGRAQVPPTS GQKRSRKIAV ISVLPNFKKP Y INDFLSLA SERLDYNYFF GNSHQINSSK ATLKTIRRMT GFVNIVNSTL SVLRTNFPLH TNSVLQPLIY SIAMAYYVLD TE STEEVHL RKMASNLRQQ GLKCLSSVFE FVGNTFDWST SMEDIYAVVV KPRISHFSDE NLQQPSSLLR LFLYWAHNPS LYQ FLYYDE FATATALMDT ISNQHVKEAV IGPIIEAADS IIRNPVNDDH YVDLVTLICT SCLKILPSLY VKLSDSNSIS TFLN LLVSI TEMGFIQDDH VRSRLISSLI SILKGKLKKL QENDTQKILK ILKLIVFNYN CSWSDIEELY TTISSLFKTF DERNL RVSL TELFIELGRK VPELESISKL VADLNSYSSS RMHEYDFPRI LSTFKGLIED GYKSYSELEW LPLLFTFLHF INNKEE LAL RTNASHAIMK FIDFINEKPN LNEASKSISM LKDILLPNIR IGLRDSLEEV QSEYVSVLSY MVKNTKYFTD FEDMAIL LY NGDEEADFFT NVNHIQLHRR QRAIKRLGEH AHQLKDNSIS HYLIPMIEHY VFSDDERYRN IGNETQIAIG GLAQHMSW N QYKALLRRYI SMLKTKPNQM KQAVQLIVQL SVPLRETLRI VRDGAESKLT LSKFPSNLDE PSNFIKQELY PTLSKILGT RDDETIIERM PIAEALVNIV LGLTNDDITN FLPSILTNIC QVLRSKSEEL RDAVRVTLGK ISIILGAEYL VFVIKELMAT LKRGSQIHV LSYTVHYILK SMHGVLKHSD LDTSSSMIVK IIMENIFGFA GEEKDSENYH TKVKEIKSNK SYDAGEILAS N ISLTEFGT LLSPVKALLM VRINLRNQNK LSELLRRYLL GLNHNSDSES ESILKFCHQL FQESEMSNSP QIPKKKVKDQ VD EKEDFFL VNLESKSYTI NSNSLLLNST LQKFALDLLR NVITRHRSFL TVSHLEGFIP FLRDSLLSEN EGVVISTLRI LIT LIRLDF SDESSEIFKN CARKVLNIIK VSPSTSSELC QMGLKFLSAF IRHTDSTLKD TALSYVLGRV LPDLNEPSRQ GLAF NFLKA LVSKHIMLPE LYDIADTTRE IMVTNHSKEI RDVSRSVYYQ FLMEYDQSKG RLEKQFKFMV DNLQYPTESG RQSVM ELIN LIITKANPAL LSKLSSSFFL ALVNVSFNDD APRCREMASV LISTMLPKLE NKDLEIVEKY IAAWLKQVDN ASFLNL GLR TYKVYLKSIG FEHTIELDEL AIKRIRYILS DTSVGSEHQW DLVYSALNTF SSYMEATESV YKHGFKDIWD GIITCLL YP HSWVRQSAAN LVHQLIANKD KLEISLTNLE IQTIATRILH QLGAPSIPEN LANVSIKTLV NISILWKEQR TPFIMDVS K QTGEDLKYTT AIDYMVTRIG GIIRSDEHRM DSFMSKKACI QLLALLVQVL DEDEVIAEGE KILLPLYGYL ETYYSRAVD EEQEELRTLS NECLKILEDK LQVSDFTKIY TAVKQTVLER RKERRSKRAI LAVNAPQISA DKKLRKHARS REKRKHEKDE NGYYQRRNK RKRA

UniProtKB: U3 small nucleolar RNA-associated protein 20

Macromolecule #51: U3 small nucleolar RNA-associated protein 14

• Macromolecule: Name: U3 small nucleolar RNA-associated protein 14 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 103.18975 KDa

Sequence

String:

MAKKKSKSRS KSSRRVLDAL QLAEREINGE FDNSSDNDKR HDARRNGTVV NLLKRSKGDT NSDEDDIDSE SFEDEELNSD EALGSDDDY DILNSKFSQT IRDKKENANY QEEEDEGGYT SIDEEDLMPL SQVWDMDEKT AQSNGNDDED ASPQLKLQDT D ISSESSSS EESESESEDD EEEEDPFDEI SEDEEDIELN TITSKLIDET KSKAPKRLDT YGSGEANEYV LPSANAASGA SG KLSLTDM MNVIDDRQVI ENANLLKGKS STYEVPLPQR IQQRHDRKAA YEISRQEVSK WNDIVQQNRR ADHLIFPLNK PTE HNHASA FTRTQDVPQT ELQEKVDQVL QESNLANPEK DSKFEELSTA KMTPEEMRKR TTEMRLMREL MFREERKARR LKKI KSKTY RKIKKKELMK NRELAAVSSD EDNEDHDIAR AKERMTLKHK TNSKWAKDMI KHGMTNDAET REEMEEMLRQ GERLK AKML DRNSDDEEDG RVQTLSDVEN EEKENIDSEA LKSKLGKTGV MNMAFMKNGE AREREANKET LRQLRAVENG DDIKLF ESD EEETNGENIQ INKGRRIYTP GSLESNKDMN ELNDHTRKEN KVDESRSLEN RLRAKNSGQS KNARTNAEGA IIVEEES DG EPLQDGQNNQ QDEEAKDVNP WLANESDEEH TVKKQSSKVN VIDKDSSKNV KAMNKMEKAE LKQKKKKKGK SNDDEDLL L TADDSTRLKI VDPYGGSDDE QGDNVFMFKQ QDVIAEAFAG DDVVAEFQEE KKRVIDDEDD KEVDTTLPGW GEWAGAGSK PKNKKRKFIK KVKGVVNKDK RRDKNLQNVI INEKVNKKNL KYQSSAVPFP FENREQYERS LRMPIGQEWT SRASHQELIK PRIMTKPGQ VIDPLKAPFK

UniProtKB: U3 small nucleolar RNA-associated protein 14

Macromolecule #52: Essential nuclear protein 1

• Macromolecule: Name: Essential nuclear protein 1 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 55.207422 KDa

Sequence

String:

MARASSTKAR KQRHDPLLKD LDAAQGTLKK INKKKLAQND AANHDAANEE DGYIDSKASR KILQLAKEQQ DEIEGEELAE SERNKQFEA RFTTMSYDDE DEDEDEDEEA FGEDISDFEP EGDYKEEEEI VEIDEEDAAM FEQYFKKSDD FNSLSGSYNL A DKIMASIR EKESQVEDMQ DDEPLANEQN TSRGNISSGL KSGEGVALPE KVIKAYTTVG SILKTWTHGK LPKLFKVIPS LR NWQDVIY VTNPEEWSPH VVYEATKLFV SNLTAKESQK FINLILLERF RDNIETSEDH SLNYHIYRAV KKSLYKPSAF FKG FLFPLV ETGCNVREAT IAGSVLAKVS VPALHSSAAL SYLLRLPFSP PTTVFIKILL DKKYALPYQT VDDCVYYFMR FRIL DDGSN GEDATRVLPV IWHKAFLTFA QRYKNDITQD QRDFLLETVR QRGHKDIGPE IRRELLAGAS REFVDPQEAN DDLMI DVN

UniProtKB: Essential nuclear protein 1

Macromolecule #53: Protein BFR2

• Macromolecule: Name: Protein BFR2 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast); Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 61.285039 KDa

Sequence

String:

MEKSLADQIS DIAIKPVNKD FDIEDEENAS LFQHNEKNGE SDLSDYGNSN TEETKKAHYL EVEKSKLRAE KGLELNDPKY TGVKGSRQA LYEEVSENED EEEEEEEEEE KEEDALSFRT DSEDEEVEID EEESDADGGE TEEAQQKRHA LSKLIQQETK Q AINKLSQS VQRDASKGYS ILQQTKLFDN IIDLRIKLQK AVIAANKLPL TTESWEEAKM DDSEETKRLL KENEKLFNNL FN RLINFRI KFQLGDHITQ NEEVAKHKLS KKRSLKELYQ ETNSLDSELK EYRTAVLNKW STKVSSASGN AALSSNKFKA INL PADVQV ENQLSDMSRL MKRTKLNRRN ITPLYFQKDC ANGRLPELIS PVVKDSVDDN ENSDDGLDIP KNYDPRRKDN NAID ITENP YVFDDEDFYR VLLNDLIDKK ISNAHNSESA AITITSTNAR SNNKLKKNID TKASKGRKLN YSVQDPIANY EAPIT SGYK WSDDQIDEFF AGLLGQRVNF NENEDEEQHA RIENDEELEA VKNDDIQIFG

UniProtKB: Protein BFR2

Macromolecule #54: Unassigned helices

• Macromolecule: Name: Unassigned helices / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: ATCC 204508 / S288c
• Molecular weight: Theoretical: 29.549291 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #55: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 55 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #56: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 56 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #57: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 57 / Number of copies: 1 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 2.0 mg/mL

Buffer

pH: 7.4
Component:

Concentration	Formula	Name
100.0 mM	CH3COOK	potassium acetate
20.0 mM	HEPES-K	HEPES-K

• Grid: Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 10 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 45 sec. / Pretreatment - Atmosphere: OTHER
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Frames/image: 1-32 / Number grids imaged: 10 / Number real images: 18028 / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.5 µm
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 382298

Startup model

Type of model: EMDB MAP
EMDB ID:

6695

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 4.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 9421
• Initial angle assignment: Type: RANDOM ASSIGNMENT / Software - Name: RELION
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION
• Final 3D classification: Number classes: 8 / Software - Name: RELION

Atomic model buiding 1

Initial model

PDB ID:

6lqr

Chain - Source name: PDB / Chain - Initial model type: experimental model

Output model

PDB-6lqv:
Cryo-EM structure of 90S small subunit preribosomes in transition states (State C1)