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基本情報
登録情報 | データベース: EMDB / ID: EMD-0821 | |||||||||
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タイトル | PSI-LHCI Supercomplex from Physcometrella patens | |||||||||
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機能・相同性 | ![]() photosystem I antenna complex / response to low light intensity stimulus / pigment binding / response to high light intensity / photosynthesis, light harvesting / plastoglobule / photosynthesis, light harvesting in photosystem I / : / photosystem I reaction center / photosystem I ...photosystem I antenna complex / response to low light intensity stimulus / pigment binding / response to high light intensity / photosynthesis, light harvesting / plastoglobule / photosynthesis, light harvesting in photosystem I / : / photosystem I reaction center / photosystem I / photosystem I / photosystem II / chlorophyll binding / photosynthetic electron transport in photosystem I / chloroplast thylakoid membrane / response to light stimulus / photosynthesis / response to cold / chloroplast / 4 iron, 4 sulfur cluster binding / membrane => GO:0016020 / electron transfer activity / oxidoreductase activity / serine-type endopeptidase activity / magnesium ion binding / protein homodimerization activity / metal ion binding 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / 解像度: 3.23 Å | |||||||||
![]() | Zhao L / Yan QJ / Qin XC | |||||||||
![]() | ![]() タイトル: Antenna arrangement and energy-transfer pathways of PSI-LHCI from the moss Physcomitrella patens. 著者: Qiujing Yan / Liang Zhao / Wenda Wang / Xiong Pi / Guangye Han / Jie Wang / Lingpeng Cheng / Yi-Kun He / Tingyun Kuang / Xiaochun Qin / Sen-Fang Sui / Jian-Ren Shen / ![]() ![]() 要旨: Plants harvest light energy utilized for photosynthesis by light-harvesting complex I and II (LHCI and LHCII) surrounding photosystem I and II (PSI and PSII), respectively. During the evolution of ...Plants harvest light energy utilized for photosynthesis by light-harvesting complex I and II (LHCI and LHCII) surrounding photosystem I and II (PSI and PSII), respectively. During the evolution of green plants, moss is at an evolutionarily intermediate position from aquatic photosynthetic organisms to land plants, being the first photosynthetic organisms that landed. Here, we report the structure of the PSI-LHCI supercomplex from the moss Physcomitrella patens (Pp) at 3.23 Å resolution solved by cryo-electron microscopy. Our structure revealed that four Lhca subunits are associated with the PSI core in an order of Lhca1-Lhca5-Lhca2-Lhca3. This number is much decreased from 8 to 10, the number of subunits in most green algal PSI-LHCI, but the same as those of land plants. Although Pp PSI-LHCI has a similar structure as PSI-LHCI of land plants, it has Lhca5, instead of Lhca4, in the second position of Lhca, and several differences were found in the arrangement of chlorophylls among green algal, moss, and land plant PSI-LHCI. One chlorophyll, PsaF-Chl 305, which is found in the moss PSI-LHCI, is located at the gap region between the two middle Lhca subunits and the PSI core, and therefore may make the excitation energy transfer from LHCI to the core more efficient than that of land plants. On the other hand, energy-transfer paths at the two side Lhca subunits are relatively conserved. These results provide a structural basis for unravelling the mechanisms of light-energy harvesting and transfer in the moss PSI-LHCI, as well as important clues on the changes of PSI-LHCI after landing. | |||||||||
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画像 | ![]() | 48 KB | ||
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「今月の分子」の関連する項目 |
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ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.061 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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試料の構成要素
+全体 : PSI-LHCI
+超分子 #1: PSI-LHCI
+分子 #1: Photosystem I P700 chlorophyll a apoprotein A1
+分子 #2: Photosystem I P700 chlorophyll a apoprotein A2
+分子 #3: Photosystem I iron-sulfur center
+分子 #4: Predicted protein PsaD
+分子 #5: PsaE
+分子 #6: PSI-F
+分子 #7: Predicted protein PsaG
+分子 #8: PsaH photosystem I reaction center subunit
+分子 #9: Photosystem I reaction center subunit VIII
+分子 #10: Photosystem I reaction center subunit IX
+分子 #11: PsaK
+分子 #12: PSI subunit V
+分子 #13: Photosystem I reaction center subunit XII
+分子 #14: Chlorophyll a-b binding protein, chloroplastic
+分子 #15: Chlorophyll a-b binding protein, chloroplastic
+分子 #16: Chlorophyll a-b binding protein, chloroplastic
+分子 #17: Chlorophyll a-b binding protein, chloroplastic
+分子 #18: CHLOROPHYLL A
+分子 #19: PHYLLOQUINONE
+分子 #20: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #21: BETA-CAROTENE
+分子 #22: IRON/SULFUR CLUSTER
+分子 #23: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #24: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #25: CHLOROPHYLL B
+分子 #26: (3R,3'R,6S)-4,5-DIDEHYDRO-5,6-DIHYDRO-BETA,BETA-CAROTENE-3,3'-DIOL
+分子 #27: (3S,5R,6S,3'S,5'R,6'S)-5,6,5',6'-DIEPOXY-5,6,5',6'- TETRAHYDRO-BE...
-実験情報
-構造解析
![]() | 単粒子再構成法 |
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試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |