+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-0523 | |||||||||
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Title | Yeast Ctf19 complex | |||||||||
Map data | Biological protomer from reconstruction of real space-subtracted images | |||||||||
Sample |
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Keywords | Kinetochore / CELL CYCLE | |||||||||
Function / homology | Function and homology information negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / COMA complex / maintenance of meiotic sister chromatid cohesion / Mis6-Sim4 complex / meiotic sister chromatid segregation / centromere complex assembly / establishment of meiotic sister chromatid cohesion / ascospore formation / attachment of spindle microtubules to kinetochore / CENP-A containing chromatin assembly ...negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / COMA complex / maintenance of meiotic sister chromatid cohesion / Mis6-Sim4 complex / meiotic sister chromatid segregation / centromere complex assembly / establishment of meiotic sister chromatid cohesion / ascospore formation / attachment of spindle microtubules to kinetochore / CENP-A containing chromatin assembly / outer kinetochore / protein localization to chromosome, centromeric region / establishment of mitotic sister chromatid cohesion / kinetochore assembly / protein localization to kinetochore / spindle pole body / mitotic spindle assembly checkpoint signaling / DNA replication initiation / meiotic cell cycle / chromosome segregation / kinetochore / cell division / structural molecule activity / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) / Saccharomyces cerevisiae S288c (yeast) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.25 Å | |||||||||
Authors | Hinshaw SM / Harrison SC | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Elife / Year: 2019 Title: The structure of the Ctf19c/CCAN from budding yeast. Authors: Stephen M Hinshaw / Stephen C Harrison / Abstract: Eukaryotic kinetochores connect spindlemicrotubules to chromosomal centromeres. A group of proteins called the Ctf19 complex (Ctf19c) in yeast and the constitutive centromere associated network (CCAN) ...Eukaryotic kinetochores connect spindlemicrotubules to chromosomal centromeres. A group of proteins called the Ctf19 complex (Ctf19c) in yeast and the constitutive centromere associated network (CCAN) in other organisms creates the foundation of a kinetochore. The Ctf19c/CCAN influences the timing of kinetochore assembly, sets its location by associating with a specialized nucleosome containing the histone H3 variant Cse4/CENP-A, and determines the organization of the microtubule attachment apparatus. We present here the structure of a reconstituted 13-subunit Ctf19c determined by cryo-electron microscopy at ~4 Å resolution. The structure accounts for known and inferred contacts with the Cse4 nucleosome and for an observed assembly hierarchy. We describe its implications for establishment of kinetochores and for their regulation by kinases throughout the cell cycle. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_0523.map.gz | 6.6 MB | EMDB map data format | |
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Header (meta data) | emd-0523-v30.xml emd-0523.xml | 27.8 KB 27.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_0523_fsc.xml | 10.9 KB | Display | FSC data file |
Images | emd_0523.png | 58.3 KB | ||
Filedesc metadata | emd-0523.cif.gz | 8.2 KB | ||
Others | emd_0523_additional.map.gz | 97.5 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-0523 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-0523 | HTTPS FTP |
-Validation report
Summary document | emd_0523_validation.pdf.gz | 382.3 KB | Display | EMDB validaton report |
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Full document | emd_0523_full_validation.pdf.gz | 381.9 KB | Display | |
Data in XML | emd_0523_validation.xml.gz | 11.9 KB | Display | |
Data in CIF | emd_0523_validation.cif.gz | 15.9 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-0523 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-0523 | HTTPS FTP |
-Related structure data
Related structure data | 6nuwMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_0523.map.gz / Format: CCP4 / Size: 107.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Biological protomer from reconstruction of real space-subtracted images | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.23 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Additional map: Dimeric assembly. Twofold symmetry enforced during reconstruction.
File | emd_0523_additional.map | ||||||||||||
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Annotation | Dimeric assembly. Twofold symmetry enforced during reconstruction. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Ctf19 complex
+Supramolecule #1: Ctf19 complex
+Macromolecule #1: Inner kinetochore subunit IML3
+Macromolecule #2: Inner kinetochore subunit MCM21
+Macromolecule #3: Inner kinetochore subunit CTF19
+Macromolecule #4: Inner kinetochore subunit CHL4
+Macromolecule #5: Inner kinetochore subunit OKP1
+Macromolecule #6: Inner kinetochore subunit NKP1
+Macromolecule #7: Inner kinetochore subunit AME1
+Macromolecule #8: Inner kinetochore subunit CTF3,Inner kinetochore subunit CTF3
+Macromolecule #9: Inner kinetochore subunit NKP2
+Macromolecule #10: Inner kinetochore subunit Mcm22
+Macromolecule #11: Inner kinetochore subunit Mcm16
+Macromolecule #12: Unknown (unassigned)
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8.5 |
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Grid | Details: unspecified |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI POLARA 300 |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 47.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Tecnai Polara / Image courtesy: FEI Company |