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Open data
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Basic information
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Title | Structure of Mycobacterium tuberculosis Fatty Acid Synthase - I | |||||||||
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![]() | Fatty Acid Synthesis / Tuberculosis / BIOSYNTHETIC PROTEIN | |||||||||
Function / homology | ![]() fatty acid synthase complex / beta-ketoacyl-[acyl-carrier-protein] synthase I / enoyl-[acyl-carrier-protein] reductase (NADH) activity / 3-oxoacyl-[acyl-carrier-protein] synthase activity / fatty acid biosynthetic process / hydrolase activity Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||
![]() | Elad N / Baron S | |||||||||
![]() | ![]() Title: Structure of Type-I Mycobacterium tuberculosis fatty acid synthase at 3.3 Å resolution. Authors: Nadav Elad / Szilvia Baron / Yoav Peleg / Shira Albeck / Jacob Grunwald / Gal Raviv / Zippora Shakked / Oren Zimhony / Ron Diskin / ![]() Abstract: Tuberculosis (TB) is a devastating and rapidly spreading disease caused by Mycobacterium tuberculosis (Mtb). Therapy requires prolonged treatment with a combination of multiple agents and ...Tuberculosis (TB) is a devastating and rapidly spreading disease caused by Mycobacterium tuberculosis (Mtb). Therapy requires prolonged treatment with a combination of multiple agents and interruptions in the treatment regimen result in emergence and spread of multi-drug resistant (MDR) Mtb strains. MDR Mtb poses a significant global health problem, calling for urgent development of novel drugs to combat TB. Here, we report the 3.3 Å resolution structure of the ~2 MDa type-I fatty acid synthase (FAS-I) from Mtb, determined by single particle cryo-EM. Mtb FAS-I is an essential enzymatic complex that contributes to the virulence of Mtb, and thus a prime target for anti-TB drugs. The structural information for Mtb FAS-I we have obtained enables computer-based drug discovery approaches, and the resolution achieved by cryo-EM is sufficient for elucidating inhibition mechanisms by putative small molecular weight inhibitors. | |||||||||
History |
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Structure visualization
Structure viewer | EM map: ![]() ![]() ![]() |
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Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 144.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 17 KB 17 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 15 KB | Display | ![]() |
Images | ![]() | 236.6 KB | ||
Masks | ![]() | 244.1 MB | ![]() | |
Filedesc metadata | ![]() | 7.1 KB | ||
Others | ![]() ![]() | 194.3 MB 194.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 507.9 KB | Display | ![]() |
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Full document | ![]() | 507.1 KB | Display | |
Data in XML | ![]() | 20 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6gjcMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.054 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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-Half map: #1
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-Half map: #2
File | emd_0011_half_map_2.map | ||||||||||||
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Sample components
-Entire : Fatty Acid Synthase - I
Entire | Name: Fatty Acid Synthase - I |
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Components |
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-Supramolecule #1: Fatty Acid Synthase - I
Supramolecule | Name: Fatty Acid Synthase - I / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Fatty acid synthase
Macromolecule | Name: Fatty acid synthase / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO / EC number: beta-ketoacyl-[acyl-carrier-protein] synthase I |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 329.35675 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MKKWSHPQFE KGGSDYKDDD DKPICTIHEH DRVSADRGGD SPHTTHALVD RLMAGEPYAV AFGGQGSAWL ETLEELVSAT GIETELATL VGEAELLLDP VTDELIVVRP IGFEPLQWVR ALAAEDPVPS DKHLTSAAVS VPGVLLTQIA ATRALARQGM D LVATPPVA ...String: MKKWSHPQFE KGGSDYKDDD DKPICTIHEH DRVSADRGGD SPHTTHALVD RLMAGEPYAV AFGGQGSAWL ETLEELVSAT GIETELATL VGEAELLLDP VTDELIVVRP IGFEPLQWVR ALAAEDPVPS DKHLTSAAVS VPGVLLTQIA ATRALARQGM D LVATPPVA MAGHSQGVLA VEALKAGGAR DVELFALAQL IGAAGTLVAR RRGISVLGDR PPMVSVTNAD PERIGRLLDE FA QDVRTVL PPVLSIRNGR RAVVITGTPE QLSRFELYCR QISEKEEADR KNKVRGGDVF SPVFEPVQVE VGFHTPRLSD GID IVAGWA EKAGLDVALA RELADAILIR KVDWVDEITR VHAAGARWIL DLGPGDILTR LTAPVIRGLG IGIVPAATRG GQRN LFTVG ATPEVARAWS SYAPTVVRLP DGRVKLSTKF TRLTGRSPIL LAGMTPTTVD AKIVAAAANA GHWAELAGGG QVTEE IFGN RIEQMAGLLE PGRTYQFNAL FLDPYLWKLQ VGGKRLVQKA RQSGAAIDGV VISAGIPDLD EAVELIDELG DIGISH VVF KPGTIEQIRS VIRIATEVPT KPVIMHVEGG RAGGHHSWED LDDLLLATYS ELRSRANITV CVGGGIGTPR RAAEYLS GR WAQAYGFPLM PIDGILVGTA AMATKESTTS PSVKRMLVDT QGTDQWISAG KAQGGMASSR SQLGADIHEI DNSASRCG R LLDEVAGDAE AVAERRDEII AAMAKTAKPY FGDVADMTYL QWLRRYVELA IGEGNSTADT ASVGSPWLAD TWRDRFEQM LQRAEARLHP QDFGPIQTLF TDAGLLDNPQ QAIAALLARY PDAETVQLHP ADVPFFVTLC KTLGKPVNFV PVIDQDVRRW WRSDSLWQA HDARYDADAV CIIPGTASVA GITRMDEPVG ELLDRFEQAA IDEVLGAGVE PKDVASRRLG RADVAGPLAV V LDAPDVRW AGRTVTNPVH RIADPAEWQV HDGPENPRAT HSSTGARLQT HGDDVALSVP VSGTWVDIRF TLPANTVDGG TP VIATEDA TSAMRTVLAI AAGVDSPEFL PAVANGTATL TVDWHPERVA DHTGVTATFG EPLAPSLTNV PDALVGPCWP AVF AAIGSA VTDTGEPVVE GLLSLVHLDH AARVVGQLPT VPAQLTVTAT AANATDTDMG RVVPVSVVVT GADGAVIATL EERF AILGR TGSAELADPA RAGGAVSANA TDTPRRRRRD VTITAPVDMR PFAVVSGDHN PIHTDRAAAL LAGLESPIVH GMWLS AAAQ HAVTATDGQA RPPARLVGWT ARFLGMVRPG DEVDFRVERV GIDQGAEIVD VAARVGSDLV MSASARLAAP KTVYAF PGQ GIQHKGMGME VRARSKAARK VWDTADKFTR DTLGFSVLHV VRDNPTSIIA SGVHYHHPDG VLYLTQFTQV AMATVAA AQ VAEMREQGAF VEGAIACGHS VGEYTALACV TGIYQLEALL EMVFHRGSKM HDIVPRDELG RSNYRLAAIR PSQIDLDD A DVPAFVAGIA ESTGEFLEIV NFNLRGSQYA IAGTVRGLEA LEAEVERRRE LTGGRRSFIL VPGIDVPFHS RVLRVGVAE FRRSLDRVMP RDADPDLIIG RYIPNLVPRL FTLDRDFIQE IRDLVPAEPL DEILADYDTW LRERPREMAR TVFIELLAWQ FASPVRWIE TQDLLFIEEA AGGLGVERFV EIGVKSSPTV AGLATNTLKL PEYAHSTVEV LNAERDAAVL FATDTDPEPE P EEDEPVAE SPAPDVVSEA APVAPAASSA GPRPDDLVFD AADATLALIA LSAKMRIDQI EELDSIESIT DGASSRRNQL LV DLGSELN LGAIDGAAES DLAGLRSQVT KLARTYKPYG PVLSDAINDQ LRTVLGPSGK RPGAIAERVK KTWELGEGWA KHV TVEVAL GTREGSSVRG GAMGHLHEGA LADAASVDKV IDAAVASVAA RQGVSVALPS AGSGGGATID AAALSEFTDQ ITGR EGVLA SAARLVLGQL GLDDPVNALP AAPDSELIDL VTAELGADWP RLVAPVFDPK KAVVFDDRWA SAREDLVKLW LTDEG DIDA DWPRLAERFE GAGHVVATQA TWWQGKSLAA GRQIHASLYG RIAAGAENPE PGRYGGEVAV VTGASKGSIA ASVVAR LLD GGATVIATTS KLDEERLAFY RTLYRDHARY GAALWLVAAN MASYSDVDAL VEWIGTEQTE SLGPQSIHIK DAQTPTL LF PFAAPRVVGD LSEAGSRAEM EMKVLLWAVQ RLIGGLSTIG AERDIASRLH VVLPGSPNRG MFGGDGAYGE AKSALDAV V SRWHAESSWA ARVSLAHALI GWTRGTGLMG HNDAIVAAVE EAGVTTYSTD EMAALLLDLC DAESKVAAAR SPIKADLTG GLAEANLDMA ELAAKAREQM SAAAAVDEDA EAPGAIAALP SPPRGFTPAP PPQWDDLDVD PADLVVIVGG AEIGPYGSSR TRFEMEVEN ELSAAGVLEL AWTTGLIRWE DDPQPGWYDT ESGEMVDESE LVQRYHDAVV QRVGIREFVD DGAIDPDHAS P LLVSVFLE KDFAFVVSSE ADARAFVEFD PEHTVIRPVP DSTDWQVIRK AGTEIRVPRK TKLSRVVGGQ IPTGFDPTVW GI SADMAGS IDRLAVWNMV ATVDAFLSSG FSPAEVMRYV HPSLVANTQG TGMGGGTSMQ TMYHGNLLGR NKPNDIFQEV LPN IIAAHV VQSYVGSYGA MIHPVAACAT AAVSVEEGVD KIRLGKAQLV VAGGLDDLTL EGIIGFGDMA ATADTSMMCG RGIH DSKFS RPNDRRRLGF VEAQGGGTIL LARGDLALRM GLPVLAVVAF AQSFGDGVHT SIPAPGLGAL GAGRGGKDSP LARAL AKLG VAADDVAVIS KHDTSTLAND PNETELHERL ADALGRSEGA PLFVVSQKSL TGHAKGGAAV FQMMGLCQIL RDGVIP PNR SLDCVDDELA GSAHFVWVRD TLRLGGKFPL KAGMLTSLGF GHVSGLVALV HPQAFIASLD PAQRADYQRR ADARLLA GQ RRLASAIAGG APMYQRPGDR RFDHHAPERP QEASMLLNPA ARLGDGEAYI UniProtKB: Fatty acid synthase |
-Macromolecule #2: FLAVIN MONONUCLEOTIDE
Macromolecule | Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 2 / Number of copies: 6 / Formula: FMN |
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Molecular weight | Theoretical: 456.344 Da |
Chemical component information | ![]() ChemComp-FMN: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.2 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 4.49 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Refinement | Protocol: FLEXIBLE FIT |
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Output model | ![]() PDB-6gjc: |