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- PDB-2uv8: Crystal structure of yeast fatty acid synthase with stalled acyl ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2uv8 | ||||||
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Title | Crystal structure of yeast fatty acid synthase with stalled acyl carrier protein at 3.1 angstrom resolution | ||||||
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![]() | TRANSFERASE / FATTY ACID BIOSYNTHESIS / MALONYL/PALMITOYL TRANSFERASE / PHOSPHOPANTETHEINE / FATTY ACID SYNTHASE / OXIDOREDUCTASE / LIPID SYNTHESIS / SUBSTRATE SHUTTLING / ACYL CARRIER PROTEIN / KETOACYL REDUCTASE / ACETYL TRANSFERASE / ENOYL REDUCTASE / PHOSPHORYLATION / KETOACYL SYNTHASE / FATTY ACID SYNTHESIS / MULTIFUNCTIONAL ENZYME / NAD / NADP / LYASE / YEAST / HYDROLASE / DEHYDRATASE | ||||||
Function / homology | ![]() : / fatty-acyl-CoA synthase system / fatty-acyl-CoA synthase activity / fatty acid synthase complex / [acyl-carrier-protein] S-acetyltransferase / palmitoyltransferase activity / (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity / [acyl-carrier-protein] S-acetyltransferase activity / fatty acyl-[ACP] hydrolase activity / oleoyl-[acyl-carrier-protein] hydrolase ...: / fatty-acyl-CoA synthase system / fatty-acyl-CoA synthase activity / fatty acid synthase complex / [acyl-carrier-protein] S-acetyltransferase / palmitoyltransferase activity / (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity / [acyl-carrier-protein] S-acetyltransferase activity / fatty acyl-[ACP] hydrolase activity / oleoyl-[acyl-carrier-protein] hydrolase / : / holo-[acyl-carrier-protein] synthase activity / [acyl-carrier-protein] S-malonyltransferase / [acyl-carrier-protein] S-malonyltransferase activity / 3-hydroxyacyl-[acyl-carrier-protein] dehydratase / beta-ketoacyl-[acyl-carrier-protein] synthase I / 3-oxoacyl-[acyl-carrier-protein] reductase / 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity / enoyl-[acyl-carrier-protein] reductase (NADH) / long-chain fatty acid biosynthetic process / fatty acid synthase activity / enoyl-[acyl-carrier-protein] reductase (NADH) activity / 3-oxoacyl-[acyl-carrier-protein] synthase activity / lipid droplet / magnesium ion binding / mitochondrion / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Leibundgut, M. / Jenni, S. / Frick, C. / Ban, N. | ||||||
![]() | ![]() Title: Structural Basis for Substrate Delivery by Acyl Carrier Protein in the Yeast Fatty Acid Synthase Authors: Leibundgut, M. / Jenni, S. / Frick, C. / Ban, N. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 2.1 MB | Display | ![]() |
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PDB format | ![]() | 1.7 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.8 MB | Display | |
Data in XML | ![]() | 404 KB | Display | |
Data in CIF | ![]() | 535.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2uvb ![]() 2uvc S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 207393.547 Da / Num. of mol.: 3 / Source method: isolated from a natural source Details: PHOSPHOPANTETHEINE PROSTHETIC GROUP COVALENTLY LINKED TO SERINE 180 OF CHAIN A, B, C Source: (natural) ![]() ![]() #2: Protein | Mass: 228940.375 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.33 Å3/Da / Density % sol: 71.38 % |
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Crystal grow | pH: 7.1 / Details: pH 7.10 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9999 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→12 Å / Num. obs: 341077 / % possible obs: 89.7 % / Observed criterion σ(I): -3 / Redundancy: 5.8 % / Biso Wilson estimate: 73.9 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 12.9 |
Reflection shell | Resolution: 3.1→3.27 Å / Rmerge(I) obs: 0.63 / Mean I/σ(I) obs: 2.4 / % possible all: 99.3 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRIES 2UVB AND 2UVC Resolution: 3.1→12 Å
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Displacement parameters | Biso mean: 72.9 Å2 | ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→12 Å
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