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Yorodumi- PDB-6wdt: Enterovirus D68 in complex with human monoclonal antibody EV68-228 -
+Open data
-Basic information
Entry | Database: PDB / ID: 6wdt | |||||||||||||||
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Title | Enterovirus D68 in complex with human monoclonal antibody EV68-228 | |||||||||||||||
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Keywords | VIRUS/IMMUNE SYSTEM / virus / enterovirus / antibody / Structural Genomics / Center for Structural Genomics of Infectious Diseases / CSGID / VIRUS-IMMUNE SYSTEM complex | |||||||||||||||
Function / homology | Function and homology information cysteine-type peptidase activity / picornain 2A / symbiont-mediated suppression of host mRNA export from nucleus / symbiont genome entry into host cell via pore formation in plasma membrane / picornain 3C / T=pseudo3 icosahedral viral capsid / host cell cytoplasmic vesicle membrane / cytoplasmic vesicle membrane / endocytosis involved in viral entry into host cell / viral capsid ...cysteine-type peptidase activity / picornain 2A / symbiont-mediated suppression of host mRNA export from nucleus / symbiont genome entry into host cell via pore formation in plasma membrane / picornain 3C / T=pseudo3 icosahedral viral capsid / host cell cytoplasmic vesicle membrane / cytoplasmic vesicle membrane / endocytosis involved in viral entry into host cell / viral capsid / nucleoside-triphosphate phosphatase / protein complex oligomerization / monoatomic ion channel activity / symbiont-mediated suppression of host gene expression / host cell cytoplasm / RNA helicase activity / symbiont entry into host cell / induction by virus of host autophagy / RNA-directed RNA polymerase / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / DNA-templated transcription / virion attachment to host cell / structural molecule activity / ATP hydrolysis activity / proteolysis / RNA binding / ATP binding / metal ion binding / cytoplasm Similarity search - Function | |||||||||||||||
Biological species | Enterovirus D68 Homo sapiens (human) | |||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||||||||
Authors | Fu, J. / Vogt, M.R. / Klose, T. / Crowe, J.E. / Rossmann, M.G. / Kuhn, R.J. / Center for Structural Genomics of Infectious Diseases (CSGID) | |||||||||||||||
Funding support | United States, 4items
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Citation | Journal: Sci Immunol / Year: 2020 Title: Human antibodies neutralize enterovirus D68 and protect against infection and paralytic disease. Authors: Matthew R Vogt / Jianing Fu / Nurgun Kose / Lauren E Williamson / Robin Bombardi / Ian Setliff / Ivelin S Georgiev / Thomas Klose / Michael G Rossmann / Yury A Bochkov / James E Gern / ...Authors: Matthew R Vogt / Jianing Fu / Nurgun Kose / Lauren E Williamson / Robin Bombardi / Ian Setliff / Ivelin S Georgiev / Thomas Klose / Michael G Rossmann / Yury A Bochkov / James E Gern / Richard J Kuhn / James E Crowe / Abstract: Enterovirus D68 (EV-D68) causes outbreaks of respiratory illness, and there is increasing evidence that it causes outbreaks of acute flaccid myelitis (AFM). There are no licensed therapies to prevent ...Enterovirus D68 (EV-D68) causes outbreaks of respiratory illness, and there is increasing evidence that it causes outbreaks of acute flaccid myelitis (AFM). There are no licensed therapies to prevent or treat EV-D68 infection or AFM disease. We isolated a panel of EV-D68-reactive human monoclonal antibodies that recognize diverse antigenic variants from participants with prior infection. One potently neutralizing cross-reactive antibody, EV68-228, protected mice from respiratory and neurologic disease when given either before or after infection. Cryo-electron microscopy studies revealed that EV68-228 and another potently neutralizing antibody (EV68-159) bound around the fivefold or threefold axes of symmetry on virion particles, respectively. The structures suggest diverse mechanisms of action by these antibodies. The high potency and effectiveness observed in vivo suggest that antibodies are a mechanistic correlate of protection against AFM disease and are candidates for clinical use in humans with EV-D68 infection. | |||||||||||||||
History |
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-Structure visualization
Movie |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6wdt.cif.gz | 188 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6wdt.ent.gz | 153.7 KB | Display | PDB format |
PDBx/mmJSON format | 6wdt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6wdt_validation.pdf.gz | 378.2 KB | Display | wwPDB validaton report |
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Full document | 6wdt_full_validation.pdf.gz | 390.8 KB | Display | |
Data in XML | 6wdt_validation.xml.gz | 21.2 KB | Display | |
Data in CIF | 6wdt_validation.cif.gz | 32.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wd/6wdt ftp://data.pdbj.org/pub/pdb/validation_reports/wd/6wdt | HTTPS FTP |
-Related structure data
Related structure data | 21648MC 6wdsC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
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Symmetry | Point symmetry: (Schoenflies symbol: I (icosahedral)) |
-Components
-Viral protein ... , 4 types, 4 molecules ABCD
#1: Protein | Mass: 32920.309 Da / Num. of mol.: 1 / Fragment: UNP residues 565-861 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterovirus D68 / Strain: US/MO/14-18047 / Cell (production host): spindle / Cell line (production host): RD / Production host: Homo sapiens (human) / Tissue (production host): muscle / References: UniProt: A0A097BW12 |
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#2: Protein | Mass: 27567.135 Da / Num. of mol.: 1 / Fragment: UNP residues 70-317 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterovirus D68 / Strain: US/MO/14-18047 / Cell (production host): spindle / Cell line (production host): RD / Production host: Homo sapiens (human) / Tissue (production host): muscle / References: UniProt: A0A0A7X639, UniProt: A0A097BW12*PLUS |
#3: Protein | Mass: 27112.814 Da / Num. of mol.: 1 / Fragment: UNP residues 318-564 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterovirus D68 / Strain: US/MO/14-18047 / Cell (production host): spindle / Cell line (production host): RD / Production host: Homo sapiens (human) / Tissue (production host): muscle / References: UniProt: A0A097BW12 |
#4: Protein | Mass: 7336.960 Da / Num. of mol.: 1 / Fragment: UNP residues 2-69 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterovirus D68 / Strain: US/MO/14-18047 / Cell (production host): spindle / Cell line (production host): RD / Production host: Homo sapiens (human) / Tissue (production host): muscle / References: UniProt: A0A126D252, UniProt: J9Z449*PLUS |
-Antibody , 2 types, 2 molecules HL
#5: Antibody | Mass: 14037.602 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster) |
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#6: Antibody | Mass: 11354.635 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster) |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
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Molecular weight | Experimental value: NO | ||||||||||||||||||||||||
Source (natural) |
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Source (recombinant) |
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Details of virus | Empty: NO / Enveloped: NO / Isolate: STRAIN / Type: VIRION | ||||||||||||||||||||||||
Buffer solution | pH: 8 | ||||||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||
Specimen support | Grid material: COPPER / Grid mesh size: 400 divisions/in. | ||||||||||||||||||||||||
Vitrification | Instrument: GATAN CRYOPLUNGE 3 / Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Cs: 2.7 mm / C2 aperture diameter: 100 µm / Alignment procedure: ZEMLIN TABLEAU |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Average exposure time: 2.6 sec. / Electron dose: 44.5 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Num. of grids imaged: 1 / Num. of real images: 462 |
EM imaging optics | Energyfilter name: GIF Bioquantum / Energyfilter slit width: 20 eV |
-Processing
Software | Name: PHENIX / Version: 1.16_3549: / Classification: refinement | ||||||||||||||||||||||||||||||
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EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||
Particle selection | Num. of particles selected: 27390 | ||||||||||||||||||||||||||||||
Symmetry | Point symmetry: I (icosahedral) | ||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 20194 / Algorithm: FOURIER SPACE / Symmetry type: POINT | ||||||||||||||||||||||||||||||
Atomic model building | Protocol: RIGID BODY FIT / Space: REAL | ||||||||||||||||||||||||||||||
Atomic model building | PDB-ID: 4WM8 | ||||||||||||||||||||||||||||||
Refine LS restraints |
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