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-基本情報
登録情報 | データベース: PDB / ID: 6bjc | ||||||||||||||||||
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タイトル | TPX2_mini decorated GMPCPP-microtubule | ||||||||||||||||||
要素 |
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キーワード | CELL CYCLE / mitosis / GanGTP / nucleation | ||||||||||||||||||
機能・相同性 | 機能・相同性情報 negative regulation of microtubule depolymerization / axon hillock / microtubule nucleation / importin-alpha family protein binding / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Carboxyterminal post-translational modifications of tubulin ...negative regulation of microtubule depolymerization / axon hillock / microtubule nucleation / importin-alpha family protein binding / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Carboxyterminal post-translational modifications of tubulin / RHOH GTPase cycle / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / PKR-mediated signaling / The role of GTSE1 in G2/M progression after G2 checkpoint / Aggrephagy / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Separation of Sister Chromatids / RHO GTPases activate IQGAPs / RHO GTPases Activate Formins / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / Recruitment of NuMA to mitotic centrosomes / COPI-mediated anterograde transport / intercellular bridge / activation of protein kinase activity / mitotic spindle assembly / regulation of mitotic spindle organization / AURKA Activation by TPX2 / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / mitotic spindle / structural constituent of cytoskeleton / spindle / spindle pole / microtubule cytoskeleton organization / microtubule cytoskeleton / mitotic cell cycle / Regulation of TP53 Activity through Phosphorylation / microtubule / molecular adaptor activity / cell division / GTPase activity / GTP binding / apoptotic process / protein kinase binding / nucleoplasm / nucleus / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | Homo sapiens (ヒト) Sus scrofa (ブタ) | ||||||||||||||||||
手法 | 電子顕微鏡法 / らせん対称体再構成法 / クライオ電子顕微鏡法 / 解像度: 3.3 Å | ||||||||||||||||||
データ登録者 | Zhang, R. / Nogales, E. | ||||||||||||||||||
資金援助 | 米国, 英国, 5件
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引用 | ジャーナル: Elife / 年: 2017 タイトル: Structural insight into TPX2-stimulated microtubule assembly. 著者: Rui Zhang / Johanna Roostalu / Thomas Surrey / Eva Nogales / 要旨: During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2. The ...During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2. The molecular mechanism of how TPX2 stimulates MT assembly remains unknown because structural information about the interaction of TPX2 with MTs is lacking. Here, we determine the cryo-electron microscopy structure of a central region of TPX2 bound to the MT surface. TPX2 uses two flexibly linked elements ('ridge' and 'wedge') in a novel interaction mode to simultaneously bind across longitudinal and lateral tubulin interfaces. These MT-interacting elements overlap with the binding site of importins on TPX2. Fluorescence microscopy-based in vitro reconstitution assays reveal that this interaction mode is critical for MT binding and facilitates MT nucleation. Together, our results suggest a molecular mechanism of how the Ran-GTP gradient can regulate TPX2-dependent MT formation. | ||||||||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6bjc.cif.gz | 1 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6bjc.ent.gz | 822.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6bjc.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 6bjc_validation.pdf.gz | 1.6 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 6bjc_full_validation.pdf.gz | 1.6 MB | 表示 | |
XML形式データ | 6bjc_validation.xml.gz | 130.6 KB | 表示 | |
CIF形式データ | 6bjc_validation.cif.gz | 197.6 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/bj/6bjc ftp://data.pdbj.org/pub/pdb/validation_reports/bj/6bjc | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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非結晶学的対称性 (NCS) | NCSドメイン:
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