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Entry | Database: PDB / ID: 6bjc | ||||||||||||||||||
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Title | TPX2_mini decorated GMPCPP-microtubule | ||||||||||||||||||
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![]() | CELL CYCLE / mitosis / GanGTP / nucleation | ||||||||||||||||||
Function / homology | ![]() microtubule nucleation / negative regulation of microtubule depolymerization / axon hillock / importin-alpha family protein binding / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Carboxyterminal post-translational modifications of tubulin ...microtubule nucleation / negative regulation of microtubule depolymerization / axon hillock / importin-alpha family protein binding / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Hedgehog 'off' state / Cilium Assembly / Intraflagellar transport / COPI-dependent Golgi-to-ER retrograde traffic / Carboxyterminal post-translational modifications of tubulin / RHOH GTPase cycle / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / PKR-mediated signaling / The role of GTSE1 in G2/M progression after G2 checkpoint / Aggrephagy / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Separation of Sister Chromatids / RHO GTPases activate IQGAPs / RHO GTPases Activate Formins / Recruitment of NuMA to mitotic centrosomes / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / COPI-mediated anterograde transport / intercellular bridge / activation of protein kinase activity / mitotic spindle assembly / regulation of mitotic spindle organization / AURKA Activation by TPX2 / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / structural constituent of cytoskeleton / mitotic spindle / spindle pole / microtubule cytoskeleton organization / spindle / microtubule cytoskeleton / mitotic cell cycle / Regulation of TP53 Activity through Phosphorylation / microtubule / molecular adaptor activity / cell division / GTPase activity / GTP binding / protein kinase binding / apoptotic process / nucleoplasm / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function | ||||||||||||||||||
Biological species | ![]() ![]() ![]() | ||||||||||||||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.3 Å | ||||||||||||||||||
![]() | Zhang, R. / Nogales, E. | ||||||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Structural insight into TPX2-stimulated microtubule assembly. Authors: Rui Zhang / Johanna Roostalu / Thomas Surrey / Eva Nogales / ![]() ![]() Abstract: During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2. The ...During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2. The molecular mechanism of how TPX2 stimulates MT assembly remains unknown because structural information about the interaction of TPX2 with MTs is lacking. Here, we determine the cryo-electron microscopy structure of a central region of TPX2 bound to the MT surface. TPX2 uses two flexibly linked elements ('ridge' and 'wedge') in a novel interaction mode to simultaneously bind across longitudinal and lateral tubulin interfaces. These MT-interacting elements overlap with the binding site of importins on TPX2. Fluorescence microscopy-based in vitro reconstitution assays reveal that this interaction mode is critical for MT binding and facilitates MT nucleation. Together, our results suggest a molecular mechanism of how the Ran-GTP gradient can regulate TPX2-dependent MT formation. | ||||||||||||||||||
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-Related structure data
Related structure data | ![]() 7101MC ![]() 7102C M: map data used to model this data C: citing same article ( |
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Noncrystallographic symmetry (NCS) | NCS domain:
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