Protein of unknown function DUF2612 / Protein of unknown function (DUF2612) / Phage protein Gp138 N-terminal domain / Phage protein Gp138 N-terminal domain / Phage spike trimer / Phage spike trimer / Baseplate protein J-like / Baseplate J-like protein / Vgr protein, OB-fold domain superfamily 類似検索 - ドメイン・相同性
Baseplate protein J-like domain-containing protein / Phage protein / Tail fiber protein / Phage protein / Baseplate protein / Putative structural protein / Structural protein / Phage protein / Phage protein 類似検索 - 構成要素
National Institutes of Health/National Cancer Institute (NIH/NCI)
GM140733
米国
引用
ジャーナル: Nat Commun / 年: 2023 タイトル: High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217. 著者: Fenglin Li / Chun-Feng David Hou / Ravi K Lokareddy / Ruoyu Yang / Francesca Forti / Federica Briani / Gino Cingolani / 要旨: E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after ...E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4.