複合体: Complex consisting of actin-filament nucleator, Arp2/3 complex associated with nucleation promoting factor Dip1 and first four actin subunits from the pointed end of the nucleated actin-filament
タンパク質・ペプチド: x 10種
リガンド: x 3種
機能・相同性
機能・相同性情報
protein localization to actin cortical patch / Regulation of actin dynamics for phagocytic cup formation / RHO GTPases Activate WASPs and WAVEs / Clathrin-mediated endocytosis / actin cortical patch organization / Neutrophil degranulation / medial cortex / actin cortical patch localization / cell cortex of cell tip / positive regulation of Arp2/3 complex-mediated actin nucleation ...protein localization to actin cortical patch / Regulation of actin dynamics for phagocytic cup formation / RHO GTPases Activate WASPs and WAVEs / Clathrin-mediated endocytosis / actin cortical patch organization / Neutrophil degranulation / medial cortex / actin cortical patch localization / cell cortex of cell tip / positive regulation of Arp2/3 complex-mediated actin nucleation / actin cortical patch assembly / Arp2/3 protein complex / Arp2/3 complex-mediated actin nucleation / Arp2/3 complex binding / cell tip / actin cortical patch / regulation of actin filament polymerization / cell septum / mating projection tip / cortical actin cytoskeleton organization / cytoskeletal motor activator activity / establishment or maintenance of cell polarity / tropomyosin binding / mesenchyme migration / cell division site / troponin I binding / myosin heavy chain binding / filamentous actin / actin filament bundle / skeletal muscle thin filament assembly / striated muscle thin filament / actin filament bundle assembly / mitotic cytokinesis / skeletal muscle myofibril / actin monomer binding / skeletal muscle fiber development / stress fiber / titin binding / actin filament polymerization / filopodium / actin filament / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / structural constituent of cytoskeleton / actin filament binding / endocytosis / calcium-dependent protein binding / lamellipodium / cell cortex / cell body / toxin activity / hydrolase activity / protein domain specific binding / calcium ion binding / positive regulation of gene expression / magnesium ion binding / ATP binding / identical protein binding / nucleus / cytoplasm / cytosol 類似検索 - 分子機能
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01GM127440, R01GM092917, S10OD012272
米国
引用
ジャーナル: Nat Struct Mol Biol / 年: 2020 タイトル: Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state. 著者: Mohammed Shaaban / Saikat Chowdhury / Brad J Nolen / 要旨: Arp2/3 complex, a crucial actin filament nucleator, undergoes structural rearrangements during activation by nucleation-promoting factors (NPFs). However, the conformational pathway leading to the ...Arp2/3 complex, a crucial actin filament nucleator, undergoes structural rearrangements during activation by nucleation-promoting factors (NPFs). However, the conformational pathway leading to the nucleation-competent state is unclear due to lack of high-resolution structures of the activated state. Here we report a ~3.9 Å resolution cryo-EM structure of activated Schizosaccharomyces pombe Arp2/3 complex bound to the S. pombe NPF Dip1 and attached to the end of the nucleated actin filament. The structure reveals global and local conformational changes that allow the two actin-related proteins in Arp2/3 complex to mimic a filamentous actin dimer and template nucleation. Activation occurs through a clamp-twisting mechanism, in which Dip1 forces two core subunits in Arp2/3 complex to pivot around one another, shifting half of the complex into a new activated position. By showing how Dip1 stimulates activation, the structure reveals how NPFs can activate Arp2/3 complex in diverse cellular processes.
全体 : Complex consisting of actin-filament nucleator, Arp2/3 complex as...
全体
名称: Complex consisting of actin-filament nucleator, Arp2/3 complex associated with nucleation promoting factor Dip1 and first four actin subunits from the pointed end of the nucleated actin-filament
要素
複合体: Complex consisting of actin-filament nucleator, Arp2/3 complex associated with nucleation promoting factor Dip1 and first four actin subunits from the pointed end of the nucleated actin-filament
タンパク質・ペプチド: Actin-related protein 3
タンパク質・ペプチド: Actin-related protein 2
タンパク質・ペプチド: Actin-related protein 2/3 complex subunit 1
タンパク質・ペプチド: Actin-related protein 2/3 complex subunit 2
タンパク質・ペプチド: Actin-related protein 2/3 complex subunit 3
タンパク質・ペプチド: Actin-related protein 2/3 complex subunit 4
タンパク質・ペプチド: Actin-related protein 2/3 complex subunit 5
タンパク質・ペプチド: Protein dip1
タンパク質・ペプチド: Actin, alpha skeletal muscle
タンパク質・ペプチド: Phalloidin
リガンド: MAGNESIUM ION
リガンド: ADENOSINE-5'-TRIPHOSPHATE
リガンド: ADENOSINE-5'-DIPHOSPHATE
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超分子 #1: Complex consisting of actin-filament nucleator, Arp2/3 complex as...
超分子
名称: Complex consisting of actin-filament nucleator, Arp2/3 complex associated with nucleation promoting factor Dip1 and first four actin subunits from the pointed end of the nucleated actin-filament タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#10
選択した数: 3500000 詳細: Particles were picked using Laplacian Gaussian auto-picking.
CTF補正
ソフトウェア - 名称: Gctf (ver. 1.06) 詳細: Particles were CTF corrected during projection matching and back projection.
初期モデル
#0 - モデルのタイプ: INSILICO MODEL #0 - in silico モデル: Ab-initio initial model was determined using CryoSPARCv2. #1 - モデルのタイプ: INSILICO MODEL #1 - in silico モデル: Ab-initio initial model was determined using CryoSPARCv2.