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- PDB-1r4u: URATE OXIDASE FROM ASPERGILLUS FLAVUS COMPLEXED WITH ITS INHIBITO... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1r4u | ||||||
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Title | URATE OXIDASE FROM ASPERGILLUS FLAVUS COMPLEXED WITH ITS INHIBITOR OXONIC ACID | ||||||
![]() | Uricase | ||||||
![]() | OXIDOREDUCTASE / URIC ACID DEGRADATION / DIMERIC BARREL / TUNNEL-SHAPED PROTEIN | ||||||
Function / homology | ![]() purine nucleobase catabolic process / urate oxidase activity / factor-independent urate hydroxylase / urate catabolic process / peroxisome Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Retailleau, P. / Colloc'h, N. / Prange, T. | ||||||
![]() | ![]() Title: Complexed and ligand-free high-resolution structures of urate oxidase (Uox) from Aspergillus flavus: a reassignment of the active-site binding mode. Authors: Retailleau, P. / Colloc'h, N. / Vivares, D. / Bonnete, F. / Castro, B. / El-Hajji, M. / Mornon, J.P. / Monard, G. / Prange, T. #1: ![]() Title: Crystal Structure of the Protein Drug Urate Oxidase-Inhibitor Complex at 2.05 A Resolution Authors: Colloc'h, N. / El Hajji, M. / Bachet, B. / L'Hermite, G. / Schiltz, M. / Prange, T. / Castro, B. / Mornon, J.-P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.2 KB | Display | ![]() |
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PDB format | ![]() | 57 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1r4sC ![]() 1r51C ![]() 1r56C ![]() 1uox C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34199.586 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() References: UniProt: Q00511, factor-independent urate hydroxylase |
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#2: Chemical | ChemComp-OXC / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.78 Å3/Da / Density % sol: 55.3 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 8.5MG/ML PROTEIN, 0.2MG/ML OXONIC ACID, 5-7% W/V PEG 8000, 100MM TRIS/HCL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 283 K | |||||||||
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Diffraction source | Source: ![]() ![]() | |||||||||
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE | |||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 1.65→20 Å / Num. obs: 48706 / % possible obs: 99.1 % / Redundancy: 8.2 % / Rmerge(I) obs: 0.064 / Net I/σ(I): 13 | |||||||||
Reflection shell | Resolution: 1.65→1.69 Å / Rmerge(I) obs: 0.375 / Mean I/σ(I) obs: 2.9 / % possible all: 95.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1UOX ![]() 1uox Resolution: 1.65→20 Å
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Refinement step | Cycle: LAST / Resolution: 1.65→20 Å
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Refine LS restraints |
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