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Yorodumi- PDB-1lvj: STRUCTURE OF TAR RNA COMPLEXED WITH A TAT-TAR INTERACTION NANOMOL... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1lvj | ||||||
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Title | STRUCTURE OF TAR RNA COMPLEXED WITH A TAT-TAR INTERACTION NANOMOLAR INHIBITOR THAT WAS IDENTIFIED BY COMPUTATIONAL SCREENING | ||||||
Components | HIV-1 Trans Activating Region RNA | ||||||
Keywords | RNA / Transcriptional Activation / Complex (RNA-drug) | ||||||
Function / homology | Chem-PMZ / RNA / RNA (> 10) Function and homology information | ||||||
Biological species | Human immunodeficiency virus 1 | ||||||
Method | SOLUTION NMR / simulated annealing starting from random coordinates | ||||||
Authors | Du, Z. / Lind, K.E. / James, T.L. | ||||||
Citation | Journal: CHEM.BIOL. / Year: 2002 Title: Structure of TAR RNA complexed with a Tat-TAR interaction nanomolar inhibitor that was identified by computational screening Authors: Du, Z. / Lind, K.E. / James, T.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1lvj.cif.gz | 250.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1lvj.ent.gz | 206.3 KB | Display | PDB format |
PDBx/mmJSON format | 1lvj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lv/1lvj ftp://data.pdbj.org/pub/pdb/validation_reports/lv/1lvj | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 9957.942 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human immunodeficiency virus 1 / Genus: Lentivirus / Description: IN Vitro Transcription |
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#2: Chemical | ChemComp-PMZ / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D NOESY, 3D 13C-seperated NOESY, 3D 15N-seperated NOESY |
-Sample preparation
Details | Contents: 10 mM sodium phosphate (pH 6.5), 20mM sodium Chloride Solvent system: 90% H2O, 10% D2O or 100% H2O |
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Sample conditions | Ionic strength: 30 mM / pH: 6.5 / Pressure: ambient / Temperature: 308 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing starting from random coordinates Software ordinal: 1 Details: The structure was determined by multidimentional heteronuclear NMR spectroscopy on a unlabeled, a 15N,13C-labeled sample and three type-specific (A,G or C) labeled samples. | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: least violations and lowest energy Conformers calculated total number: 12 / Conformers submitted total number: 12 |