+Open data
-Basic information
Entry | Database: PDB / ID: 1dgf | ||||||
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Title | HUMAN ERYTHROCYTE CATALASE | ||||||
Components | CATALASE | ||||||
Keywords | OXIDOREDUCTASE / CATALASE / HEME / NADPH / HYDROGEN PEROXIDE | ||||||
Function / homology | Function and homology information response to phenylpropanoid / aminoacylase activity / catalase complex / hemoglobin metabolic process / response to inactivity / cellular detoxification of hydrogen peroxide / response to ozone / oxidoreductase activity, acting on peroxide as acceptor / response to L-ascorbic acid / catalase ...response to phenylpropanoid / aminoacylase activity / catalase complex / hemoglobin metabolic process / response to inactivity / cellular detoxification of hydrogen peroxide / response to ozone / oxidoreductase activity, acting on peroxide as acceptor / response to L-ascorbic acid / catalase / UV protection / response to fatty acid / response to light intensity / catalase activity / response to vitamin A / peroxisomal membrane / ureteric bud development / triglyceride metabolic process / Detoxification of Reactive Oxygen Species / antioxidant activity / peroxisomal matrix / positive regulation of cell division / response to vitamin E / response to hyperoxia / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / response to cadmium ion / cholesterol metabolic process / response to activity / response to reactive oxygen species / hydrogen peroxide catabolic process / Peroxisomal protein import / response to lead ion / response to insulin / response to hydrogen peroxide / aerobic respiration / cellular response to growth factor stimulus / osteoblast differentiation / response to estradiol / peroxisome / NADP binding / secretory granule lumen / response to ethanol / ficolin-1-rich granule lumen / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / response to hypoxia / response to xenobiotic stimulus / intracellular membrane-bounded organelle / focal adhesion / heme binding / Neutrophil degranulation / negative regulation of apoptotic process / enzyme binding / protein homodimerization activity / protein-containing complex / mitochondrion / extracellular exosome / extracellular region / identical protein binding / membrane / metal ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.5 Å | ||||||
Authors | Putnam, C.D. / Arvai, A.S. / Bourne, Y. / Tainer, J.A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2000 Title: Active and inhibited human catalase structures: ligand and NADPH binding and catalytic mechanism. Authors: Putnam, C.D. / Arvai, A.S. / Bourne, Y. / Tainer, J.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dgf.cif.gz | 454.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dgf.ent.gz | 367.1 KB | Display | PDB format |
PDBx/mmJSON format | 1dgf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1dgf_validation.pdf.gz | 2.5 MB | Display | wwPDB validaton report |
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Full document | 1dgf_full_validation.pdf.gz | 2.6 MB | Display | |
Data in XML | 1dgf_validation.xml.gz | 92.8 KB | Display | |
Data in CIF | 1dgf_validation.cif.gz | 141.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dg/1dgf ftp://data.pdbj.org/pub/pdb/validation_reports/dg/1dgf | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 56626.359 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell: ERYTHROCYTE / References: UniProt: P04040, catalase #2: Chemical | ChemComp-ACT / #3: Chemical | ChemComp-HEM / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.05 Å3/Da / Density % sol: 59.72 % | ||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 6.5 - 8.0% PEG4000, PROTEIN AT 40 MG/ML IN 50MM TRISCL, PH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 200 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL7-1 / Wavelength: 1.08 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Mar 6, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→30 Å / Num. all: 401102 / Num. obs: 401102 / % possible obs: 90.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 2.8 % / Biso Wilson estimate: 14.2 Å2 / Rsym value: 0.045 / Net I/σ(I): 20 |
Reflection shell | Resolution: 1.5→1.55 Å / Redundancy: 2.8 % / Rsym value: 0.279 / % possible all: 82.2 |
Reflection | *PLUS Num. measured all: 1131638 / Rmerge(I) obs: 0.045 |
Reflection shell | *PLUS % possible obs: 82.2 % / Rmerge(I) obs: 0.279 / Mean I/σ(I) obs: 2.9 |
-Processing
Software |
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Refinement | Resolution: 1.5→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: ENGH & HUBER Details: Bond Angles and Lengths to the Heme Iron from both the TYR358 ligand and the Heme Nitrogens were unconstrained
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Displacement parameters | Biso mean: 15.35 Å2
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Refinement step | Cycle: LAST / Resolution: 1.5→20 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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