ジャーナル: Proc Natl Acad Sci U S A / 年: 2008 タイトル: Crystal structure of CD155 and electron microscopic studies of its complexes with polioviruses. 著者: Ping Zhang / Steffen Mueller / Marc C Morais / Carol M Bator / Valorie D Bowman / Susan Hafenstein / Eckard Wimmer / Michael G Rossmann / 要旨: When poliovirus (PV) recognizes its receptor, CD155, the virus changes from a 160S to a 135S particle before releasing its genome into the cytoplasm. CD155 is a transmembrane protein with 3 Ig-like ...When poliovirus (PV) recognizes its receptor, CD155, the virus changes from a 160S to a 135S particle before releasing its genome into the cytoplasm. CD155 is a transmembrane protein with 3 Ig-like extracellular domains, D1-D3, where D1 is recognized by the virus. The crystal structure of D1D2 has been determined to 3.5-A resolution and fitted into approximately 8.5-A resolution cryoelectron microscopy reconstructions of the virus-receptor complexes for the 3 PV serotypes. These structures show that, compared with human rhinoviruses, the virus-receptor interactions for PVs have a greater dependence on hydrophobic interactions, as might be required for a virus that can inhabit environments of different pH. The pocket factor was shown to remain in the virus during the first recognition stage. The present structures, when combined with earlier mutational investigations, show that in the subsequent entry stage the receptor moves further into the canyon when at a physiological temperature, thereby expelling the pocket factor and separating the viral subunits to form 135S particles. These results provide a detailed analysis of how a nonenveloped virus can enter its host cell.
#200 - 2016年8月 正二十面体型ウイルスの準対称性 (Quasisymmetry in Icosahedral Viruses) 類似性 (1)
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マップ
ファイル
ダウンロード / ファイル: emd_1562.map.gz / 形式: CCP4 / 大きさ: 38.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
CryoEM reconstructions of PV3 complexed with deglycosylated CD155
ボクセルのサイズ
X=Y=Z: 2.64 Å
密度
表面レベル
登録者による: 7.55 / ムービー #1: 6
最小 - 最大
-8.472036360000001 - 35.176879880000001
平均 (標準偏差)
1.77997804 (±5.76821709)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-108
-108
-108
サイズ
217
217
217
Spacing
217
217
217
セル
A=B=C: 572.88 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
2.64
2.64
2.64
M x/y/z
217
217
217
origin x/y/z
0.000
0.000
0.000
length x/y/z
572.880
572.880
572.880
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-81
-81
-81
NX/NY/NZ
160
160
160
MAP C/R/S
1
2
3
start NC/NR/NS
-108
-108
-108
NC/NR/NS
217
217
217
D min/max/mean
-8.472
35.177
1.780
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添付データ
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試料の構成要素
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全体 : CD155-PV3 complex
全体
名称: CD155-PV3 complex
要素
試料: CD155-PV3 complex
ウイルス: Human poliovirus 3 (ポリオウイルス)
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超分子 #1000: CD155-PV3 complex
超分子
名称: CD155-PV3 complex / タイプ: sample / ID: 1000 集合状態: 60 copies of CD155 bind to icosahedral protein shell of a PV3 particle Number unique components: 2
分子量
理論値: 11.1 MDa
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超分子 #1: Human poliovirus 3
超分子
名称: Human poliovirus 3 / タイプ: virus / ID: 1 / Name.synonym: poliovirus type 3 / NCBI-ID: 12086 / 生物種: Human poliovirus 3 / ウイルスタイプ: VIRION / ウイルス・単離状態: SEROTYPE / ウイルス・エンベロープ: No / ウイルス・中空状態: No / Syn species name: poliovirus type 3
宿主
生物種: Homo sapiens (ヒト) / 別称: VERTEBRATES
分子量
理論値: 8.5 MDa
ウイルス殻
Shell ID: 1 / 直径: 310 Å
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実験情報
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構造解析
手法
ネガティブ染色法, クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
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試料調製
濃度
2 mg/mL
緩衝液
pH: 7.5 / 詳細: 10mM Tris-HCl, 20mM NaCl
染色
タイプ: NEGATIVE 詳細: A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine ...詳細: A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.
想定した対称性 - 点群: I (正20面体型対称) / 解像度のタイプ: BY AUTHOR / 解像度: 9.0 Å / 解像度の算出法: FSC 0.5 CUT-OFF 詳細: Final map includes data to 9.0 Ang resolution (fsc 0.5 cut-off), magnification of final map standardized to a map calculated from PV3 atomic coordinates (PDB accession no 1PVC) resulting in ...詳細: Final map includes data to 9.0 Ang resolution (fsc 0.5 cut-off), magnification of final map standardized to a map calculated from PV3 atomic coordinates (PDB accession no 1PVC) resulting in final pixel separation of 2.64 Ang