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- EMDB-1418: Binding of a neutralizing antibody to dengue virus alters the arr... -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-1418 | |||||||||
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Title | Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins | |||||||||
![]() | This is a map of Fab 1A1D-2 complexed with Dengue virus | |||||||||
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Function / homology | ![]() immunoglobulin complex, circulating / immunoglobulin receptor binding / complement activation, classical pathway / antigen binding / antibacterial humoral response / viral nucleocapsid / clathrin-dependent endocytosis of virus by host cell / blood microparticle / host cell endoplasmic reticulum membrane / protein dimerization activity ...immunoglobulin complex, circulating / immunoglobulin receptor binding / complement activation, classical pathway / antigen binding / antibacterial humoral response / viral nucleocapsid / clathrin-dependent endocytosis of virus by host cell / blood microparticle / host cell endoplasmic reticulum membrane / protein dimerization activity / symbiont-mediated suppression of host innate immune response / virus-mediated perturbation of host defense response / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / virion membrane / extracellular exosome / extracellular region / membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 24.0 Å | |||||||||
![]() | Lok SM | |||||||||
![]() | ![]() Title: Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins. Authors: Shee-Mei Lok / Victor Kostyuchenko / Grant E Nybakken / Heather A Holdaway / Anthony J Battisti / Soila Sukupolvi-Petty / Dagmar Sedlak / Daved H Fremont / Paul R Chipman / John T Roehrig / ...Authors: Shee-Mei Lok / Victor Kostyuchenko / Grant E Nybakken / Heather A Holdaway / Anthony J Battisti / Soila Sukupolvi-Petty / Dagmar Sedlak / Daved H Fremont / Paul R Chipman / John T Roehrig / Michael S Diamond / Richard J Kuhn / Michael G Rossmann / ![]() Abstract: The monoclonal antibody 1A1D-2 has been shown to strongly neutralize dengue virus serotypes 1, 2 and 3, primarily by inhibiting attachment to host cells. A crystal structure of its antigen binding ...The monoclonal antibody 1A1D-2 has been shown to strongly neutralize dengue virus serotypes 1, 2 and 3, primarily by inhibiting attachment to host cells. A crystal structure of its antigen binding fragment (Fab) complexed with domain III of the viral envelope glycoprotein, E, showed that the epitope would be partially occluded in the known structure of the mature dengue virus. Nevertheless, antibody could bind to the virus at 37 degrees C, suggesting that the virus is in dynamic motion making hidden epitopes briefly available. A cryo-electron microscope image reconstruction of the virus:Fab complex showed large changes in the organization of the E protein that exposed the epitopes on two of the three E molecules in each of the 60 icosahedral asymmetric units of the virus. The changes in the structure of the viral surface are presumably responsible for inhibiting attachment to cells. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 59.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.7 KB 11.7 KB | Display Display | ![]() |
Images | ![]() | 118.9 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2r6pMC ![]() 2r29C ![]() 2r69C M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | This is a map of Fab 1A1D-2 complexed with Dengue virus | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.74 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Fab Fragment of MAb 1A1D-2 complexed with Dengue 2 virus
Entire | Name: Fab Fragment of MAb 1A1D-2 complexed with Dengue 2 virus |
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Components |
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-Supramolecule #1000: Fab Fragment of MAb 1A1D-2 complexed with Dengue 2 virus
Supramolecule | Name: Fab Fragment of MAb 1A1D-2 complexed with Dengue 2 virus type: sample / ID: 1000 Oligomeric state: The Fab molecule binds to two of the three E proteins in the icosahedral assymmetric unit Number unique components: 2 |
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-Supramolecule #1: Dengue virus 2
Supramolecule | Name: Dengue virus 2 / type: virus / ID: 1 / Name.synonym: DENV / NCBI-ID: 11060 / Sci species name: Dengue virus 2 / Virus type: VIRION / Virus isolate: SEROTYPE / Virus enveloped: Yes / Virus empty: No / Syn species name: DENV |
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Host (natural) | Organism: ![]() |
Virus shell | Shell ID: 1 / Name: envelope / Diameter: 240 Å / T number (triangulation number): 3 |
-Macromolecule #1: Fab fragment of MAb 1A1D-2
Macromolecule | Name: Fab fragment of MAb 1A1D-2 / type: protein_or_peptide / ID: 1 / Name.synonym: 1A1D-2 / Number of copies: 2 / Oligomeric state: heterodimer / Recombinant expression: No |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Experimental: 50 MDa / Theoretical: 50 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.6 mg/mL |
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Buffer | pH: 7.6 / Details: 12 mM Tris-HCl, 120 mM NaCl, 1 mM EDTA |
Grid | Details: 400 mesh copper grid |
Vitrification | Cryogen name: ETHANE / Instrument: HOMEMADE PLUNGER Details: Vitrification instrument: Guillotine-style plunge freezeing device Method: A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine ...Method: A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope. |
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Electron microscopy
Microscope | FEI/PHILIPS CM200T |
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Temperature | Average: 98 K |
Alignment procedure | Legacy - Astigmatism: live FFT |
Details | low dose |
Date | Mar 21, 2007 |
Image recording | Category: CCD / Film or detector model: KODAK SO-163 FILM / Digitization - Sampling interval: 7 µm / Average electron dose: 24 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Calibrated magnification: 51040 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.0 mm / Nominal defocus max: 3.37 µm / Nominal defocus min: 2.27 µm / Nominal magnification: 50000 |
Sample stage | Specimen holder: Eucentric / Specimen holder model: GATAN LIQUID NITROGEN |
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Image processing
CTF correction | Details: each particle |
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Final reconstruction | Applied symmetry - Point group: I (icosahedral) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 24.0 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: spider,XMIPP / Number images used: 2885 |
-Atomic model buiding 1
Initial model | PDB ID: Chain - Chain ID - 0: 2 / Chain - Chain ID - 1: R / Chain - Chain ID - 2: 2 / Chain - Chain ID - 3: 9 |
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Software | Name: EMFIT |
Details | Protocol: Rigid body |
Refinement | Protocol: RIGID BODY FIT |
Output model | ![]() PDB-2r6p: |