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基本情報
登録情報 | データベース: EMDB / ID: EMD-12808 | |||||||||
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タイトル | Cryo-EM structure of yeast Ost6p containing oligosaccharyltransferase complex | |||||||||
![]() | Local resolution filtered map from RELION | |||||||||
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機能・相同性 | ![]() Miscellaneous transport and binding events / oligosaccharyltransferase I complex / dolichol-linked oligosaccharide biosynthetic process / oligosaccharyltransferase complex / dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / protein N-linked glycosylation via asparagine / protein N-linked glycosylation / glycosyltransferase activity / protein-disulfide reductase activity ...Miscellaneous transport and binding events / oligosaccharyltransferase I complex / dolichol-linked oligosaccharide biosynthetic process / oligosaccharyltransferase complex / dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / protein N-linked glycosylation via asparagine / protein N-linked glycosylation / glycosyltransferase activity / protein-disulfide reductase activity / Neutrophil degranulation / post-translational protein modification / nuclear envelope / protein-macromolecule adaptor activity / endoplasmic reticulum membrane / structural molecule activity / endoplasmic reticulum / mitochondrion / metal ion binding 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.46 Å | |||||||||
![]() | Wild R / Neuhaus JD / Eyring J / Irobalieva RN / Kowal J / Lin CW / Locher KP / Aebi M | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Functional analysis of Ost3p and Ost6p containing yeast oligosaccharyltransferases. 要旨: The oligosaccharyltransferase (OST) is the central enzyme in the N-glycosylation pathway. It transfers a defined oligosaccharide from a lipid-linker onto the asparagine side chain of proteins. The ...The oligosaccharyltransferase (OST) is the central enzyme in the N-glycosylation pathway. It transfers a defined oligosaccharide from a lipid-linker onto the asparagine side chain of proteins. The yeast OST consists of eight subunits and exists in two catalytically distinct isoforms that differ in one subunit, Ost3p or Ost6p. The cryo-electron microscopy structure of the Ost6p containing complex was found to be highly similar to the Ost3p containing OST. OST enzymes with altered Ost3p/Ost6p subunits were generated and functionally analyzed. The three C-terminal transmembrane helices were responsible for the higher turnover-rate of the Ost3p vs. the Ost6p containing enzyme in vitro and the more severe hypoglycosylation in Ost3p lacking strains in vivo. Glycosylation of specific OST target sites required the N-terminal thioredoxin domain of Ost3p or Ost6p. This Ost3p/Ost6p dependence was glycosylation site but not protein specific. We concluded that the Ost3p/Ost6p subunits modulate the catalytic activity of OST and provide additional specificity for OST substrate recognition. | |||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
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マップデータ | ![]() | 132.8 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 26.3 KB 26.3 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 17.6 KB | 表示 | ![]() |
画像 | ![]() | 153.8 KB | ||
マスクデータ | ![]() | 216 MB | ![]() | |
その他 | ![]() ![]() | 16.2 MB 156.2 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Local resolution filtered map from RELION | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.84 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-マスク #1
ファイル | ![]() | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Postprocessed map from RELION
ファイル | emd_12808_additional_1.map | ||||||||||||
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注釈 | Postprocessed map from RELION | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Gaussian filtered map containing the Ost6p luminal domain
ファイル | emd_12808_additional_2.map | ||||||||||||
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注釈 | Gaussian filtered map containing the Ost6p luminal domain | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Ost6p containing yeast oligosaccharyltransferase complex
+超分子 #1: Ost6p containing yeast oligosaccharyltransferase complex
+分子 #1: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase su...
+分子 #2: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase su...
+分子 #3: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase su...
+分子 #4: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase su...
+分子 #5: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase su...
+分子 #6: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase su...
+分子 #7: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase su...
+分子 #8: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase su...
+分子 #9: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase su...
+分子 #12: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
+分子 #13: 2-acetamido-2-deoxy-beta-D-glucopyranose
+分子 #14: Digitonin
+分子 #15: MAGNESIUM ION
+分子 #16: Dolichylphosphate
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 5 mg/mL |
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緩衝液 | pH: 7.5 |
グリッド | モデル: Quantifoil R1.2/1.3 / 材質: COPPER / メッシュ: 300 |
凍結 | 凍結剤: ETHANE-PROPANE |
詳細 | This sample was monodisperse |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 67.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | C2レンズ絞り径: 2.7 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |